Experiment: RB23
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rnhA and yafS overlap by 4 nucleotides yafS and gloB are separated by 33 nucleotides gloB and mltD are separated by 209 nucleotides
HEPCGN_07910: rnhA - ribonuclease HI, at 320,743 to 321,210
rnhA
HEPCGN_07915: yafS - Uncharacterized protein YafS, at 321,207 to 321,929
yafS
HEPCGN_07920: gloB - hydroxyacylglutathione hydrolase, at 321,963 to 322,718
gloB
HEPCGN_07925: mltD - murein transglycosylase D, at 322,928 to 324,148
mltD
Position (kb) 321
322
323 Strain fitness (log2 ratio) -2
-1
0
1
2 at 320.991 kb on + strand, within rnhA at 321.222 kb on - strand at 321.326 kb on + strand, within yafS at 321.326 kb on + strand, within yafS at 321.334 kb on - strand, within yafS at 321.417 kb on + strand, within yafS at 321.468 kb on - strand, within yafS at 321.482 kb on + strand, within yafS at 321.482 kb on + strand, within yafS at 321.482 kb on + strand, within yafS at 321.483 kb on - strand, within yafS at 321.484 kb on + strand, within yafS at 321.485 kb on - strand, within yafS at 321.554 kb on + strand, within yafS at 321.635 kb on + strand, within yafS at 321.636 kb on - strand, within yafS at 321.636 kb on - strand, within yafS at 321.957 kb on + strand at 321.957 kb on + strand at 321.957 kb on + strand at 321.958 kb on - strand at 321.963 kb on - strand at 321.971 kb on + strand at 321.972 kb on - strand at 321.999 kb on + strand at 321.999 kb on + strand at 321.999 kb on + strand at 321.999 kb on + strand at 322.000 kb on - strand at 322.000 kb on - strand at 322.000 kb on - strand at 322.108 kb on + strand, within gloB at 322.186 kb on - strand, within gloB at 322.186 kb on - strand, within gloB at 322.186 kb on - strand, within gloB at 322.208 kb on + strand, within gloB at 322.209 kb on - strand, within gloB at 322.304 kb on + strand, within gloB at 322.305 kb on - strand, within gloB at 322.444 kb on + strand, within gloB at 322.461 kb on + strand, within gloB at 322.565 kb on - strand, within gloB at 322.710 kb on - strand at 322.710 kb on - strand at 322.945 kb on + strand at 323.039 kb on + strand at 323.044 kb on - strand at 323.050 kb on + strand, within mltD at 323.091 kb on - strand, within mltD at 323.092 kb on + strand, within mltD at 323.092 kb on + strand, within mltD at 323.092 kb on + strand, within mltD at 323.092 kb on + strand, within mltD at 323.092 kb on + strand, within mltD at 323.093 kb on - strand, within mltD at 323.093 kb on - strand, within mltD at 323.093 kb on - strand, within mltD at 323.093 kb on - strand, within mltD at 323.097 kb on - strand, within mltD at 323.097 kb on - strand, within mltD at 323.146 kb on - strand, within mltD at 323.148 kb on + strand, within mltD at 323.148 kb on + strand, within mltD at 323.148 kb on + strand, within mltD at 323.149 kb on - strand, within mltD at 323.149 kb on - strand, within mltD at 323.157 kb on + strand, within mltD at 323.243 kb on + strand, within mltD at 323.373 kb on - strand, within mltD at 323.373 kb on - strand, within mltD at 323.374 kb on + strand, within mltD at 323.375 kb on - strand, within mltD at 323.407 kb on + strand, within mltD at 323.477 kb on + strand, within mltD at 323.478 kb on - strand, within mltD
Per-strain Table
Position Strand Gene LocusTag Fraction RB23 remove 320,991 + rnhA HEPCGN_07910 0.53 +0.8 321,222 - -1.9 321,326 + yafS HEPCGN_07915 0.16 -0.3 321,326 + yafS HEPCGN_07915 0.16 -0.7 321,334 - yafS HEPCGN_07915 0.18 +0.2 321,417 + yafS HEPCGN_07915 0.29 +0.9 321,468 - yafS HEPCGN_07915 0.36 -0.5 321,482 + yafS HEPCGN_07915 0.38 +0.3 321,482 + yafS HEPCGN_07915 0.38 +1.8 321,482 + yafS HEPCGN_07915 0.38 +0.3 321,483 - yafS HEPCGN_07915 0.38 -0.5 321,484 + yafS HEPCGN_07915 0.38 +0.3 321,485 - yafS HEPCGN_07915 0.38 +0.3 321,554 + yafS HEPCGN_07915 0.48 -0.1 321,635 + yafS HEPCGN_07915 0.59 +0.3 321,636 - yafS HEPCGN_07915 0.59 -0.4 321,636 - yafS HEPCGN_07915 0.59 +0.3 321,957 + -0.0 321,957 + -0.5 321,957 + -0.6 321,958 - -0.1 321,963 - -1.2 321,971 + +0.3 321,972 - -0.0 321,999 + -0.1 321,999 + +0.9 321,999 + +0.3 321,999 + +0.3 322,000 - -0.0 322,000 - -0.9 322,000 - +0.4 322,108 + gloB HEPCGN_07920 0.19 +1.1 322,186 - gloB HEPCGN_07920 0.29 -1.0 322,186 - gloB HEPCGN_07920 0.29 -0.5 322,186 - gloB HEPCGN_07920 0.29 +0.3 322,208 + gloB HEPCGN_07920 0.32 -0.4 322,209 - gloB HEPCGN_07920 0.33 +0.0 322,304 + gloB HEPCGN_07920 0.45 +0.3 322,305 - gloB HEPCGN_07920 0.45 +0.5 322,444 + gloB HEPCGN_07920 0.64 +0.1 322,461 + gloB HEPCGN_07920 0.66 -0.5 322,565 - gloB HEPCGN_07920 0.80 +0.9 322,710 - +0.9 322,710 - -1.0 322,945 + +0.2 323,039 + +0.3 323,044 - +0.8 323,050 + mltD HEPCGN_07925 0.10 +0.4 323,091 - mltD HEPCGN_07925 0.13 -0.0 323,092 + mltD HEPCGN_07925 0.13 -1.7 323,092 + mltD HEPCGN_07925 0.13 +1.1 323,092 + mltD HEPCGN_07925 0.13 +0.2 323,092 + mltD HEPCGN_07925 0.13 +1.1 323,092 + mltD HEPCGN_07925 0.13 +2.5 323,093 - mltD HEPCGN_07925 0.14 +0.4 323,093 - mltD HEPCGN_07925 0.14 +0.1 323,093 - mltD HEPCGN_07925 0.14 -0.8 323,093 - mltD HEPCGN_07925 0.14 +0.3 323,097 - mltD HEPCGN_07925 0.14 -0.2 323,097 - mltD HEPCGN_07925 0.14 -0.7 323,146 - mltD HEPCGN_07925 0.18 +1.6 323,148 + mltD HEPCGN_07925 0.18 +0.5 323,148 + mltD HEPCGN_07925 0.18 -0.2 323,148 + mltD HEPCGN_07925 0.18 +0.1 323,149 - mltD HEPCGN_07925 0.18 -1.1 323,149 - mltD HEPCGN_07925 0.18 -1.1 323,157 + mltD HEPCGN_07925 0.19 +0.7 323,243 + mltD HEPCGN_07925 0.26 +1.2 323,373 - mltD HEPCGN_07925 0.36 +0.9 323,373 - mltD HEPCGN_07925 0.36 +0.2 323,374 + mltD HEPCGN_07925 0.37 +1.2 323,375 - mltD HEPCGN_07925 0.37 +0.5 323,407 + mltD HEPCGN_07925 0.39 -0.5 323,477 + mltD HEPCGN_07925 0.45 +1.1 323,478 - mltD HEPCGN_07925 0.45 +0.6
Or see this region's nucleotide sequence