Experiment: RB23
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt flhB and flhA overlap by 8 nucleotides flhA and flhe overlap by 1 nucleotides flhe and argS are separated by 52 nucleotides
HEPCGN_04585: flhB - flagellar biosynthesis protein FlhB, at 797,605 to 798,753
flhB
HEPCGN_04590: flhA - flagellar biosynthesis protein FlhA, at 798,746 to 800,824
flhA
HEPCGN_04595: flhe - flagellar protein FlhE, at 800,824 to 801,216
flhe
HEPCGN_04600: argS - arginine--tRNA ligase, at 801,269 to 803,002
argS
Position (kb) 798
799
800
801 Strain fitness (log2 ratio) -2
-1
0
1
2 at 797.811 kb on + strand, within flhB at 797.811 kb on + strand, within flhB at 798.042 kb on + strand, within flhB at 798.526 kb on - strand, within flhB at 798.693 kb on + strand at 798.693 kb on + strand at 798.694 kb on - strand at 798.694 kb on - strand at 798.853 kb on - strand at 798.890 kb on - strand at 798.899 kb on - strand at 799.009 kb on + strand, within flhA at 799.009 kb on + strand, within flhA at 799.010 kb on - strand, within flhA at 799.222 kb on + strand, within flhA at 799.352 kb on - strand, within flhA at 799.352 kb on - strand, within flhA at 799.469 kb on + strand, within flhA at 799.471 kb on + strand, within flhA at 799.471 kb on + strand, within flhA at 799.472 kb on - strand, within flhA at 799.624 kb on - strand, within flhA at 799.783 kb on + strand, within flhA at 799.784 kb on - strand, within flhA at 799.903 kb on + strand, within flhA at 799.976 kb on - strand, within flhA at 800.025 kb on + strand, within flhA at 800.092 kb on - strand, within flhA at 800.221 kb on + strand, within flhA at 800.222 kb on - strand, within flhA at 800.414 kb on - strand, within flhA at 800.715 kb on + strand at 800.716 kb on - strand at 800.752 kb on - strand at 800.782 kb on + strand at 800.791 kb on + strand at 800.798 kb on - strand at 800.823 kb on - strand at 800.835 kb on - strand at 800.838 kb on - strand at 800.862 kb on - strand at 800.898 kb on + strand, within flhe at 800.910 kb on + strand, within flhe at 801.071 kb on + strand, within flhe at 801.203 kb on + strand at 801.260 kb on - strand at 801.260 kb on - strand at 801.260 kb on - strand at 801.271 kb on - strand at 801.271 kb on - strand at 801.271 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RB23 remove 797,811 + flhB HEPCGN_04585 0.18 +0.2 797,811 + flhB HEPCGN_04585 0.18 -0.8 798,042 + flhB HEPCGN_04585 0.38 -0.7 798,526 - flhB HEPCGN_04585 0.80 +0.5 798,693 + +0.4 798,693 + -0.1 798,694 - +0.1 798,694 - -1.9 798,853 - -0.5 798,890 - -0.9 798,899 - -0.2 799,009 + flhA HEPCGN_04590 0.13 +0.3 799,009 + flhA HEPCGN_04590 0.13 -1.3 799,010 - flhA HEPCGN_04590 0.13 +1.0 799,222 + flhA HEPCGN_04590 0.23 -1.8 799,352 - flhA HEPCGN_04590 0.29 -0.3 799,352 - flhA HEPCGN_04590 0.29 +0.7 799,469 + flhA HEPCGN_04590 0.35 +0.3 799,471 + flhA HEPCGN_04590 0.35 -0.0 799,471 + flhA HEPCGN_04590 0.35 +0.2 799,472 - flhA HEPCGN_04590 0.35 -0.1 799,624 - flhA HEPCGN_04590 0.42 +0.8 799,783 + flhA HEPCGN_04590 0.50 +0.9 799,784 - flhA HEPCGN_04590 0.50 -0.4 799,903 + flhA HEPCGN_04590 0.56 +1.4 799,976 - flhA HEPCGN_04590 0.59 +1.0 800,025 + flhA HEPCGN_04590 0.62 +0.6 800,092 - flhA HEPCGN_04590 0.65 +0.5 800,221 + flhA HEPCGN_04590 0.71 -1.1 800,222 - flhA HEPCGN_04590 0.71 +1.0 800,414 - flhA HEPCGN_04590 0.80 +0.2 800,715 + +0.0 800,716 - -0.5 800,752 - -0.0 800,782 + -0.6 800,791 + +0.1 800,798 - -0.2 800,823 - -1.0 800,835 - -1.0 800,838 - -0.7 800,862 - +0.1 800,898 + flhe HEPCGN_04595 0.19 +1.0 800,910 + flhe HEPCGN_04595 0.22 +0.1 801,071 + flhe HEPCGN_04595 0.63 +1.0 801,203 + -1.3 801,260 - -0.4 801,260 - +0.4 801,260 - +0.4 801,271 - -0.1 801,271 - +2.0 801,271 - -1.6
Or see this region's nucleotide sequence