Experiment: RB23
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fur and uof overlap by 8 nucleotides uof and fldA are separated by 209 nucleotides fldA and ybfE are separated by 139 nucleotides ybfE and ybfF are separated by 70 nucleotides
HEPCGN_01095: fur - ferric iron uptake transcriptional regulator, at 144,778 to 145,224
fur
HEPCGN_01100: uof - fur leader peptide, at 145,217 to 145,303
uof
HEPCGN_01105: fldA - flavodoxin FldA, at 145,513 to 146,043
fldA
HEPCGN_01110: ybfE - LexA regulated protein, at 146,183 to 146,545
ybfE
HEPCGN_01115: ybfF - esterase, at 146,616 to 147,380
ybfF
Position (kb) 146
147 Strain fitness (log2 ratio) -2
-1
0
1
2 at 145.242 kb on - strand, within uof at 145.307 kb on - strand at 145.358 kb on + strand at 145.377 kb on - strand at 145.377 kb on - strand at 145.382 kb on + strand at 145.383 kb on - strand at 145.383 kb on - strand at 145.383 kb on - strand at 145.488 kb on - strand at 146.068 kb on - strand at 146.166 kb on + strand at 146.226 kb on - strand, within ybfE at 146.257 kb on + strand, within ybfE at 146.257 kb on + strand, within ybfE at 146.257 kb on + strand, within ybfE at 146.336 kb on + strand, within ybfE at 146.366 kb on - strand, within ybfE at 146.368 kb on + strand, within ybfE at 146.371 kb on + strand, within ybfE at 146.444 kb on + strand, within ybfE at 146.444 kb on + strand, within ybfE at 146.444 kb on + strand, within ybfE at 146.445 kb on - strand, within ybfE at 146.445 kb on - strand, within ybfE at 146.452 kb on + strand, within ybfE at 146.538 kb on + strand at 146.541 kb on + strand at 146.541 kb on + strand at 146.542 kb on - strand at 146.604 kb on + strand at 146.731 kb on - strand, within ybfF at 146.922 kb on - strand, within ybfF at 147.018 kb on + strand, within ybfF at 147.018 kb on + strand, within ybfF at 147.079 kb on - strand, within ybfF at 147.087 kb on - strand, within ybfF at 147.125 kb on + strand, within ybfF at 147.126 kb on - strand, within ybfF at 147.191 kb on - strand, within ybfF at 147.234 kb on - strand, within ybfF at 147.248 kb on + strand, within ybfF at 147.248 kb on + strand, within ybfF at 147.248 kb on + strand, within ybfF at 147.248 kb on + strand, within ybfF at 147.248 kb on + strand, within ybfF at 147.249 kb on - strand, within ybfF at 147.279 kb on + strand, within ybfF at 147.336 kb on - strand at 147.416 kb on + strand at 147.416 kb on + strand at 147.417 kb on - strand at 147.419 kb on + strand at 147.419 kb on + strand at 147.419 kb on + strand at 147.419 kb on + strand at 147.420 kb on - strand at 147.420 kb on - strand at 147.453 kb on + strand at 147.454 kb on - strand at 147.511 kb on + strand at 147.518 kb on - strand at 147.518 kb on - strand at 147.520 kb on - strand at 147.520 kb on - strand at 147.543 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RB23 remove 145,242 - uof HEPCGN_01100 0.29 -1.7 145,307 - -1.6 145,358 + -0.3 145,377 - +0.4 145,377 - +0.6 145,382 + -0.5 145,383 - +0.7 145,383 - +1.4 145,383 - +1.1 145,488 - -2.7 146,068 - +0.2 146,166 + -0.4 146,226 - ybfE HEPCGN_01110 0.12 +0.3 146,257 + ybfE HEPCGN_01110 0.20 +0.4 146,257 + ybfE HEPCGN_01110 0.20 +1.9 146,257 + ybfE HEPCGN_01110 0.20 +0.1 146,336 + ybfE HEPCGN_01110 0.42 -0.2 146,366 - ybfE HEPCGN_01110 0.50 +1.1 146,368 + ybfE HEPCGN_01110 0.51 -0.5 146,371 + ybfE HEPCGN_01110 0.52 +0.7 146,444 + ybfE HEPCGN_01110 0.72 +0.6 146,444 + ybfE HEPCGN_01110 0.72 +0.0 146,444 + ybfE HEPCGN_01110 0.72 +1.6 146,445 - ybfE HEPCGN_01110 0.72 +1.1 146,445 - ybfE HEPCGN_01110 0.72 -0.2 146,452 + ybfE HEPCGN_01110 0.74 +0.2 146,538 + +0.9 146,541 + -1.1 146,541 + +0.6 146,542 - +0.9 146,604 + -0.3 146,731 - ybfF HEPCGN_01115 0.15 +0.7 146,922 - ybfF HEPCGN_01115 0.40 +0.4 147,018 + ybfF HEPCGN_01115 0.53 -0.1 147,018 + ybfF HEPCGN_01115 0.53 +0.7 147,079 - ybfF HEPCGN_01115 0.61 -0.3 147,087 - ybfF HEPCGN_01115 0.62 -0.4 147,125 + ybfF HEPCGN_01115 0.67 +0.0 147,126 - ybfF HEPCGN_01115 0.67 -0.5 147,191 - ybfF HEPCGN_01115 0.75 -0.2 147,234 - ybfF HEPCGN_01115 0.81 -0.3 147,248 + ybfF HEPCGN_01115 0.83 +1.5 147,248 + ybfF HEPCGN_01115 0.83 +1.2 147,248 + ybfF HEPCGN_01115 0.83 -0.7 147,248 + ybfF HEPCGN_01115 0.83 -0.1 147,248 + ybfF HEPCGN_01115 0.83 -0.4 147,249 - ybfF HEPCGN_01115 0.83 -0.9 147,279 + ybfF HEPCGN_01115 0.87 -0.0 147,336 - +0.6 147,416 + -0.2 147,416 + -0.1 147,417 - -1.5 147,419 + -1.7 147,419 + +1.1 147,419 + -1.1 147,419 + -0.1 147,420 - -0.3 147,420 - +0.2 147,453 + -0.5 147,454 - -0.3 147,511 + -1.1 147,518 - -0.8 147,518 - +0.7 147,520 - +0.2 147,520 - +0.7 147,543 + +0.1
Or see this region's nucleotide sequence