Experiment: RB23
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HEPCGN_00225 and HEPCGN_00230 overlap by 4 nucleotides HEPCGN_00230 and HEPCGN_00235 overlap by 14 nucleotides HEPCGN_00235 and HEPCGN_00240 are separated by 453 nucleotides HEPCGN_00240 and HEPCGN_00245 are separated by 51 nucleotides HEPCGN_00245 and HEPCGN_00250 overlap by 4 nucleotides HEPCGN_00250 and HEPCGN_00255 are separated by 82 nucleotides
HEPCGN_00225: HEPCGN_00225 - hypothetical protein, at 14,273 to 14,815
_00225
HEPCGN_00230: HEPCGN_00230 - hypothetical protein, at 14,812 to 15,063
_00230
HEPCGN_00235: HEPCGN_00235 - XRE family transcriptional regulator, at 15,050 to 15,277
_00235
HEPCGN_00240: HEPCGN_00240 - hypothetical protein, at 15,731 to 16,030
_00240
HEPCGN_00245: HEPCGN_00245 - Phage tail protein, at 16,082 to 16,456
_00245
HEPCGN_00250: HEPCGN_00250 - hypothetical protein, at 16,453 to 16,764
_00250
HEPCGN_00255: HEPCGN_00255 - hypothetical protein, at 16,847 to 16,984
_00255
Position (kb) 15
16
17 Strain fitness (log2 ratio) -2
-1
0
1 at 14.773 kb on - strand at 14.938 kb on + strand, within HEPCGN_00230 at 14.939 kb on - strand, within HEPCGN_00230 at 15.296 kb on + strand at 15.296 kb on + strand at 15.297 kb on - strand at 15.297 kb on - strand at 15.297 kb on - strand at 15.301 kb on + strand at 15.301 kb on + strand at 15.302 kb on - strand at 15.323 kb on - strand at 15.705 kb on + strand at 15.705 kb on + strand at 15.806 kb on - strand, within HEPCGN_00240 at 15.837 kb on - strand, within HEPCGN_00240 at 15.837 kb on - strand, within HEPCGN_00240 at 15.837 kb on - strand, within HEPCGN_00240 at 16.136 kb on - strand, within HEPCGN_00245 at 16.529 kb on + strand, within HEPCGN_00250 at 16.730 kb on + strand, within HEPCGN_00250 at 16.970 kb on + strand, within HEPCGN_00255
Per-strain Table
Position Strand Gene LocusTag Fraction RB23 remove 14,773 - -2.0 14,938 + HEPCGN_00230 0.50 +0.2 14,939 - HEPCGN_00230 0.50 -1.4 15,296 + -0.8 15,296 + -0.5 15,297 - -0.6 15,297 - -0.9 15,297 - -0.1 15,301 + +0.2 15,301 + -1.0 15,302 - -0.4 15,323 - -0.2 15,705 + -0.1 15,705 + +0.9 15,806 - HEPCGN_00240 0.25 +0.6 15,837 - HEPCGN_00240 0.35 +0.0 15,837 - HEPCGN_00240 0.35 +1.3 15,837 - HEPCGN_00240 0.35 +0.4 16,136 - HEPCGN_00245 0.14 -0.3 16,529 + HEPCGN_00250 0.24 +0.3 16,730 + HEPCGN_00250 0.89 -0.1 16,970 + HEPCGN_00255 0.89 -0.1
Or see this region's nucleotide sequence