Experiment: RB23
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt flgC and flgD are separated by 11 nucleotides flgD and flgE are separated by 24 nucleotides flgE and flgF are separated by 19 nucleotides flgF and flgG are separated by 136 nucleotides
HEPCGN_23820: flgC - flagellar basal body rod protein FlgC, at 3,553,170 to 3,553,574
flgC
HEPCGN_23825: flgD - flagellar hook assembly protein FlgD, at 3,553,586 to 3,554,281
flgD
HEPCGN_23830: flgE - flagellar hook protein FlgE, at 3,554,306 to 3,555,508
flgE
HEPCGN_23835: flgF - flagellar basal-body rod protein FlgF, at 3,555,528 to 3,556,283
flgF
HEPCGN_23840: flgG - flagellar basal-body rod protein FlgG, at 3,556,420 to 3,557,202
flgG
Position (kb)
3554
3555
3556 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3553.312 kb on + strand, within flgC at 3553.402 kb on - strand, within flgC at 3553.438 kb on - strand, within flgC at 3553.503 kb on + strand, within flgC at 3553.585 kb on + strand at 3553.585 kb on + strand at 3553.585 kb on + strand at 3553.642 kb on + strand at 3553.646 kb on - strand at 3553.785 kb on + strand, within flgD at 3554.083 kb on + strand, within flgD at 3554.121 kb on - strand, within flgD at 3554.279 kb on + strand at 3554.362 kb on + strand at 3554.393 kb on + strand at 3554.585 kb on + strand, within flgE at 3554.586 kb on - strand, within flgE at 3554.635 kb on + strand, within flgE at 3554.806 kb on + strand, within flgE at 3554.833 kb on + strand, within flgE at 3554.837 kb on + strand, within flgE at 3554.838 kb on - strand, within flgE at 3554.879 kb on - strand, within flgE at 3554.890 kb on + strand, within flgE at 3554.891 kb on - strand, within flgE at 3554.891 kb on - strand, within flgE at 3554.947 kb on + strand, within flgE at 3554.996 kb on - strand, within flgE at 3555.038 kb on - strand, within flgE at 3555.098 kb on - strand, within flgE at 3555.098 kb on - strand, within flgE at 3555.174 kb on - strand, within flgE at 3555.210 kb on - strand, within flgE at 3555.243 kb on + strand, within flgE at 3555.436 kb on + strand at 3555.440 kb on + strand at 3555.522 kb on - strand at 3555.744 kb on + strand, within flgF at 3555.745 kb on - strand, within flgF at 3555.986 kb on + strand, within flgF at 3556.285 kb on + strand at 3556.285 kb on + strand at 3556.308 kb on - strand at 3556.407 kb on + strand at 3556.408 kb on - strand at 3556.506 kb on + strand, within flgG
Per-strain Table
Position Strand Gene LocusTag Fraction RB23 remove 3,553,312 + flgC HEPCGN_23820 0.35 -0.7 3,553,402 - flgC HEPCGN_23820 0.57 +2.0 3,553,438 - flgC HEPCGN_23820 0.66 +0.2 3,553,503 + flgC HEPCGN_23820 0.82 +2.2 3,553,585 + -2.0 3,553,585 + +1.0 3,553,585 + -1.1 3,553,642 + -0.9 3,553,646 - +0.7 3,553,785 + flgD HEPCGN_23825 0.29 -0.3 3,554,083 + flgD HEPCGN_23825 0.71 +0.7 3,554,121 - flgD HEPCGN_23825 0.77 +0.8 3,554,279 + +1.5 3,554,362 + -1.2 3,554,393 + +0.4 3,554,585 + flgE HEPCGN_23830 0.23 -1.0 3,554,586 - flgE HEPCGN_23830 0.23 -0.6 3,554,635 + flgE HEPCGN_23830 0.27 +0.3 3,554,806 + flgE HEPCGN_23830 0.42 +0.1 3,554,833 + flgE HEPCGN_23830 0.44 -0.5 3,554,837 + flgE HEPCGN_23830 0.44 -1.5 3,554,838 - flgE HEPCGN_23830 0.44 -0.8 3,554,879 - flgE HEPCGN_23830 0.48 +1.1 3,554,890 + flgE HEPCGN_23830 0.49 -0.1 3,554,891 - flgE HEPCGN_23830 0.49 -0.6 3,554,891 - flgE HEPCGN_23830 0.49 +0.7 3,554,947 + flgE HEPCGN_23830 0.53 -0.5 3,554,996 - flgE HEPCGN_23830 0.57 -0.4 3,555,038 - flgE HEPCGN_23830 0.61 -0.5 3,555,098 - flgE HEPCGN_23830 0.66 -0.8 3,555,098 - flgE HEPCGN_23830 0.66 +0.6 3,555,174 - flgE HEPCGN_23830 0.72 -0.1 3,555,210 - flgE HEPCGN_23830 0.75 +0.0 3,555,243 + flgE HEPCGN_23830 0.78 +2.2 3,555,436 + -0.5 3,555,440 + -0.3 3,555,522 - -0.2 3,555,744 + flgF HEPCGN_23835 0.29 -0.7 3,555,745 - flgF HEPCGN_23835 0.29 -0.1 3,555,986 + flgF HEPCGN_23835 0.61 +0.1 3,556,285 + +0.2 3,556,285 + +0.1 3,556,308 - +1.0 3,556,407 + +0.6 3,556,408 - -0.1 3,556,506 + flgG HEPCGN_23840 0.11 -0.6
Or see this region's nucleotide sequence