Strain Fitness in Escherichia coli ECOR38 around HEPCGN_22950

Experiment: RB23

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntftsK and lolA are separated by 158 nucleotideslolA and rarA are separated by 10 nucleotidesrarA and serS are separated by 90 nucleotides HEPCGN_22940: ftsK - DNA translocase FtsK, at 3,366,721 to 3,370,827 ftsK HEPCGN_22945: lolA - outer membrane lipoprotein chaperone LolA, at 3,370,986 to 3,371,597 lolA HEPCGN_22950: rarA - replication-associated recombination protein RarA, at 3,371,608 to 3,372,951 rarA HEPCGN_22955: serS - serine--tRNA ligase, at 3,373,042 to 3,374,334 serS Position (kb) 3371 3372 3373Strain fitness (log2 ratio) -2 -1 0 1 2 3at 3370.825 kb on + strandat 3370.830 kb on - strandat 3370.839 kb on + strandat 3370.970 kb on - strandat 3371.592 kb on - strandat 3371.592 kb on - strandat 3371.592 kb on - strandat 3371.641 kb on - strandat 3371.641 kb on - strandat 3371.681 kb on + strandat 3371.689 kb on + strandat 3371.690 kb on - strandat 3371.691 kb on + strandat 3371.763 kb on + strand, within rarAat 3371.763 kb on + strand, within rarAat 3371.763 kb on + strand, within rarAat 3371.764 kb on - strand, within rarAat 3371.764 kb on - strand, within rarAat 3371.847 kb on + strand, within rarAat 3371.847 kb on + strand, within rarAat 3371.848 kb on - strand, within rarAat 3371.848 kb on - strand, within rarAat 3371.848 kb on - strand, within rarAat 3371.932 kb on - strand, within rarAat 3371.995 kb on - strand, within rarAat 3371.995 kb on - strand, within rarAat 3371.995 kb on - strand, within rarAat 3372.105 kb on + strand, within rarAat 3372.114 kb on + strand, within rarAat 3372.246 kb on + strand, within rarAat 3372.246 kb on + strand, within rarAat 3372.292 kb on - strand, within rarAat 3372.314 kb on + strand, within rarAat 3372.339 kb on + strand, within rarAat 3372.355 kb on - strand, within rarAat 3372.374 kb on - strand, within rarAat 3372.396 kb on + strand, within rarAat 3372.396 kb on + strand, within rarAat 3372.397 kb on - strand, within rarAat 3372.433 kb on + strand, within rarAat 3372.473 kb on + strand, within rarAat 3372.473 kb on + strand, within rarAat 3372.473 kb on + strand, within rarAat 3372.474 kb on - strand, within rarAat 3372.474 kb on - strand, within rarAat 3372.475 kb on + strand, within rarAat 3372.475 kb on + strand, within rarAat 3372.475 kb on + strand, within rarAat 3372.476 kb on - strand, within rarAat 3372.476 kb on - strand, within rarAat 3372.655 kb on + strand, within rarAat 3372.727 kb on - strand, within rarAat 3372.743 kb on + strand, within rarAat 3372.743 kb on + strand, within rarAat 3372.743 kb on + strand, within rarAat 3372.802 kb on + strand, within rarAat 3372.850 kb on + strandat 3372.927 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RB23
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3,370,825 + +0.1
3,370,830 - +1.2
3,370,839 + +2.2
3,370,970 - -1.6
3,371,592 - +0.2
3,371,592 - -0.9
3,371,592 - -0.0
3,371,641 - +0.3
3,371,641 - +0.5
3,371,681 + +1.0
3,371,689 + +0.5
3,371,690 - +0.2
3,371,691 + -1.3
3,371,763 + rarA HEPCGN_22950 0.12 -0.2
3,371,763 + rarA HEPCGN_22950 0.12 -0.2
3,371,763 + rarA HEPCGN_22950 0.12 +1.2
3,371,764 - rarA HEPCGN_22950 0.12 +0.4
3,371,764 - rarA HEPCGN_22950 0.12 -1.5
3,371,847 + rarA HEPCGN_22950 0.18 -0.1
3,371,847 + rarA HEPCGN_22950 0.18 -0.1
3,371,848 - rarA HEPCGN_22950 0.18 -0.1
3,371,848 - rarA HEPCGN_22950 0.18 -0.1
3,371,848 - rarA HEPCGN_22950 0.18 +0.4
3,371,932 - rarA HEPCGN_22950 0.24 -0.9
3,371,995 - rarA HEPCGN_22950 0.29 -0.4
3,371,995 - rarA HEPCGN_22950 0.29 -1.3
3,371,995 - rarA HEPCGN_22950 0.29 +0.2
3,372,105 + rarA HEPCGN_22950 0.37 +1.8
3,372,114 + rarA HEPCGN_22950 0.38 -0.6
3,372,246 + rarA HEPCGN_22950 0.47 -2.6
3,372,246 + rarA HEPCGN_22950 0.47 +0.9
3,372,292 - rarA HEPCGN_22950 0.51 -1.2
3,372,314 + rarA HEPCGN_22950 0.53 +0.1
3,372,339 + rarA HEPCGN_22950 0.54 +0.2
3,372,355 - rarA HEPCGN_22950 0.56 +0.6
3,372,374 - rarA HEPCGN_22950 0.57 -0.9
3,372,396 + rarA HEPCGN_22950 0.59 +1.2
3,372,396 + rarA HEPCGN_22950 0.59 -0.3
3,372,397 - rarA HEPCGN_22950 0.59 -1.3
3,372,433 + rarA HEPCGN_22950 0.61 +0.7
3,372,473 + rarA HEPCGN_22950 0.64 -0.5
3,372,473 + rarA HEPCGN_22950 0.64 -2.4
3,372,473 + rarA HEPCGN_22950 0.64 -0.2
3,372,474 - rarA HEPCGN_22950 0.64 +0.4
3,372,474 - rarA HEPCGN_22950 0.64 -1.7
3,372,475 + rarA HEPCGN_22950 0.65 -0.4
3,372,475 + rarA HEPCGN_22950 0.65 +0.0
3,372,475 + rarA HEPCGN_22950 0.65 -0.6
3,372,476 - rarA HEPCGN_22950 0.65 -0.9
3,372,476 - rarA HEPCGN_22950 0.65 -0.8
3,372,655 + rarA HEPCGN_22950 0.78 +0.7
3,372,727 - rarA HEPCGN_22950 0.83 +2.8
3,372,743 + rarA HEPCGN_22950 0.84 +0.1
3,372,743 + rarA HEPCGN_22950 0.84 +0.0
3,372,743 + rarA HEPCGN_22950 0.84 -0.8
3,372,802 + rarA HEPCGN_22950 0.89 -0.1
3,372,850 + +0.1
3,372,927 + -0.9

Or see this region's nucleotide sequence