Experiment: RB23
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mlaF and mlaE are separated by 7 nucleotides mlaE and mlaD are separated by 4 nucleotides mlaD and mlaC are separated by 18 nucleotides mlaC and mlaC overlap by 1 nucleotides
HEPCGN_16850: mlaF - phospholipid ABC transporter ATP-binding protein MlaF, at 2,121,923 to 2,122,732
mlaF
HEPCGN_16855: mlaE - lipid asymmetry maintenance ABC transporter permease subunit MlaE, at 2,122,740 to 2,123,522
mlaE
HEPCGN_16860: mlaD - outer membrane lipid asymmetry maintenance protein MlaD, at 2,123,527 to 2,124,078
mlaD
HEPCGN_16865: mlaC - phospholipid-binding protein MlaC, at 2,124,097 to 2,124,732
mlaC
HEPCGN_16870: mlaC - phospholipid-binding protein MlaC, at 2,124,732 to 2,125,025
mlaC
Position (kb)
2123
2124
2125 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2122.548 kb on + strand, within mlaF at 2122.549 kb on - strand, within mlaF at 2122.549 kb on - strand, within mlaF at 2122.549 kb on - strand, within mlaF at 2122.549 kb on - strand, within mlaF at 2122.552 kb on + strand, within mlaF at 2122.641 kb on + strand, within mlaF at 2122.662 kb on + strand at 2122.748 kb on - strand at 2122.748 kb on - strand at 2122.814 kb on - strand at 2122.886 kb on + strand, within mlaE at 2122.913 kb on + strand, within mlaE at 2122.962 kb on + strand, within mlaE at 2122.980 kb on + strand, within mlaE at 2122.980 kb on + strand, within mlaE at 2122.980 kb on + strand, within mlaE at 2122.981 kb on - strand, within mlaE at 2122.981 kb on - strand, within mlaE at 2122.981 kb on - strand, within mlaE at 2122.982 kb on + strand, within mlaE at 2122.982 kb on + strand, within mlaE at 2122.982 kb on + strand, within mlaE at 2123.132 kb on + strand, within mlaE at 2123.195 kb on + strand, within mlaE at 2123.204 kb on - strand, within mlaE at 2123.220 kb on - strand, within mlaE at 2123.220 kb on - strand, within mlaE at 2123.351 kb on + strand, within mlaE at 2123.352 kb on - strand, within mlaE at 2123.570 kb on + strand at 2123.627 kb on + strand, within mlaD at 2123.628 kb on - strand, within mlaD at 2123.631 kb on + strand, within mlaD at 2123.632 kb on - strand, within mlaD at 2123.632 kb on - strand, within mlaD at 2123.644 kb on + strand, within mlaD at 2123.645 kb on - strand, within mlaD at 2123.645 kb on - strand, within mlaD at 2123.645 kb on - strand, within mlaD at 2123.646 kb on + strand, within mlaD at 2123.646 kb on + strand, within mlaD at 2123.647 kb on - strand, within mlaD at 2123.759 kb on - strand, within mlaD at 2123.794 kb on + strand, within mlaD at 2123.857 kb on + strand, within mlaD at 2123.868 kb on - strand, within mlaD at 2123.989 kb on - strand, within mlaD at 2123.989 kb on - strand, within mlaD at 2124.003 kb on + strand, within mlaD at 2124.003 kb on + strand, within mlaD at 2124.004 kb on - strand, within mlaD at 2124.004 kb on - strand, within mlaD at 2124.067 kb on - strand at 2124.103 kb on - strand at 2124.110 kb on + strand at 2124.111 kb on - strand at 2124.178 kb on + strand, within mlaC at 2124.180 kb on + strand, within mlaC at 2124.181 kb on - strand, within mlaC at 2124.295 kb on + strand, within mlaC at 2124.298 kb on - strand, within mlaC at 2124.299 kb on + strand, within mlaC at 2124.300 kb on - strand, within mlaC at 2124.300 kb on - strand, within mlaC at 2124.300 kb on - strand, within mlaC at 2124.340 kb on + strand, within mlaC at 2124.341 kb on - strand, within mlaC at 2124.373 kb on + strand, within mlaC at 2124.373 kb on + strand, within mlaC at 2124.430 kb on + strand, within mlaC at 2124.431 kb on - strand, within mlaC at 2124.431 kb on - strand, within mlaC at 2124.493 kb on - strand, within mlaC at 2124.586 kb on + strand, within mlaC at 2124.612 kb on + strand, within mlaC at 2124.612 kb on + strand, within mlaC at 2124.654 kb on + strand, within mlaC at 2124.848 kb on + strand, within mlaC at 2124.961 kb on + strand, within mlaC at 2124.962 kb on - strand, within mlaC at 2124.975 kb on + strand, within mlaC at 2124.975 kb on + strand, within mlaC at 2124.976 kb on - strand, within mlaC
Per-strain Table
Position Strand Gene LocusTag Fraction RB23 remove 2,122,548 + mlaF HEPCGN_16850 0.77 -2.2 2,122,549 - mlaF HEPCGN_16850 0.77 +1.3 2,122,549 - mlaF HEPCGN_16850 0.77 -0.6 2,122,549 - mlaF HEPCGN_16850 0.77 -1.6 2,122,549 - mlaF HEPCGN_16850 0.77 -0.2 2,122,552 + mlaF HEPCGN_16850 0.78 +0.7 2,122,641 + mlaF HEPCGN_16850 0.89 -1.2 2,122,662 + -0.0 2,122,748 - -1.8 2,122,748 - -1.9 2,122,814 - -1.9 2,122,886 + mlaE HEPCGN_16855 0.19 -1.7 2,122,913 + mlaE HEPCGN_16855 0.22 -0.9 2,122,962 + mlaE HEPCGN_16855 0.28 -2.8 2,122,980 + mlaE HEPCGN_16855 0.31 -1.5 2,122,980 + mlaE HEPCGN_16855 0.31 -1.5 2,122,980 + mlaE HEPCGN_16855 0.31 -1.9 2,122,981 - mlaE HEPCGN_16855 0.31 -1.6 2,122,981 - mlaE HEPCGN_16855 0.31 -0.4 2,122,981 - mlaE HEPCGN_16855 0.31 -0.0 2,122,982 + mlaE HEPCGN_16855 0.31 -2.2 2,122,982 + mlaE HEPCGN_16855 0.31 -1.5 2,122,982 + mlaE HEPCGN_16855 0.31 +0.6 2,123,132 + mlaE HEPCGN_16855 0.50 -2.0 2,123,195 + mlaE HEPCGN_16855 0.58 -1.5 2,123,204 - mlaE HEPCGN_16855 0.59 -0.0 2,123,220 - mlaE HEPCGN_16855 0.61 -1.3 2,123,220 - mlaE HEPCGN_16855 0.61 -1.9 2,123,351 + mlaE HEPCGN_16855 0.78 -2.3 2,123,352 - mlaE HEPCGN_16855 0.78 -1.4 2,123,570 + -1.7 2,123,627 + mlaD HEPCGN_16860 0.18 -1.5 2,123,628 - mlaD HEPCGN_16860 0.18 -1.7 2,123,631 + mlaD HEPCGN_16860 0.19 -1.7 2,123,632 - mlaD HEPCGN_16860 0.19 -0.7 2,123,632 - mlaD HEPCGN_16860 0.19 -1.9 2,123,644 + mlaD HEPCGN_16860 0.21 -1.0 2,123,645 - mlaD HEPCGN_16860 0.21 -0.0 2,123,645 - mlaD HEPCGN_16860 0.21 -1.2 2,123,645 - mlaD HEPCGN_16860 0.21 -1.2 2,123,646 + mlaD HEPCGN_16860 0.22 -1.5 2,123,646 + mlaD HEPCGN_16860 0.22 -0.8 2,123,647 - mlaD HEPCGN_16860 0.22 -1.0 2,123,759 - mlaD HEPCGN_16860 0.42 -2.6 2,123,794 + mlaD HEPCGN_16860 0.48 -0.8 2,123,857 + mlaD HEPCGN_16860 0.60 -0.7 2,123,868 - mlaD HEPCGN_16860 0.62 -1.6 2,123,989 - mlaD HEPCGN_16860 0.84 -1.2 2,123,989 - mlaD HEPCGN_16860 0.84 -2.0 2,124,003 + mlaD HEPCGN_16860 0.86 -0.2 2,124,003 + mlaD HEPCGN_16860 0.86 -2.6 2,124,004 - mlaD HEPCGN_16860 0.86 -1.3 2,124,004 - mlaD HEPCGN_16860 0.86 -1.7 2,124,067 - -0.5 2,124,103 - -1.4 2,124,110 + +1.9 2,124,111 - -1.2 2,124,178 + mlaC HEPCGN_16865 0.13 -0.5 2,124,180 + mlaC HEPCGN_16865 0.13 +0.1 2,124,181 - mlaC HEPCGN_16865 0.13 -1.1 2,124,295 + mlaC HEPCGN_16865 0.31 -0.5 2,124,298 - mlaC HEPCGN_16865 0.32 -3.1 2,124,299 + mlaC HEPCGN_16865 0.32 -0.2 2,124,300 - mlaC HEPCGN_16865 0.32 -0.6 2,124,300 - mlaC HEPCGN_16865 0.32 -1.7 2,124,300 - mlaC HEPCGN_16865 0.32 -0.9 2,124,340 + mlaC HEPCGN_16865 0.38 -1.7 2,124,341 - mlaC HEPCGN_16865 0.38 +0.0 2,124,373 + mlaC HEPCGN_16865 0.43 +0.7 2,124,373 + mlaC HEPCGN_16865 0.43 -3.4 2,124,430 + mlaC HEPCGN_16865 0.52 -1.2 2,124,431 - mlaC HEPCGN_16865 0.53 -2.6 2,124,431 - mlaC HEPCGN_16865 0.53 -1.0 2,124,493 - mlaC HEPCGN_16865 0.62 -0.9 2,124,586 + mlaC HEPCGN_16865 0.77 -2.4 2,124,612 + mlaC HEPCGN_16865 0.81 -0.5 2,124,612 + mlaC HEPCGN_16865 0.81 -2.2 2,124,654 + mlaC HEPCGN_16865 0.88 -2.5 2,124,848 + mlaC HEPCGN_16870 0.39 -1.1 2,124,961 + mlaC HEPCGN_16870 0.78 -0.5 2,124,962 - mlaC HEPCGN_16870 0.78 -1.1 2,124,975 + mlaC HEPCGN_16870 0.83 -1.0 2,124,975 + mlaC HEPCGN_16870 0.83 +0.3 2,124,976 - mlaC HEPCGN_16870 0.83 +0.5
Or see this region's nucleotide sequence