Experiment: RB23
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt srkA and yihD are separated by 76 nucleotides yihD and mobA are separated by 69 nucleotides mobA and mobB overlap by 4 nucleotides mobB and 5S_rrna are separated by 102 nucleotides 5S_rrna and 23S_rrna are separated by 93 nucleotides
HEPCGN_12810: srkA - stress response kinase SrkA, at 1,312,309 to 1,313,295
srkA
HEPCGN_12815: yihD - Protein YihD, at 1,313,372 to 1,313,641
yihD
HEPCGN_12820: mobA - molybdenum cofactor guanylyltransferase MobA, at 1,313,711 to 1,314,295
mobA
HEPCGN_12825: mobB - molybdopterin-guanine dinucleotide biosynthesis protein MobB, at 1,314,292 to 1,314,804
mobB
HEPCGN_12830: 5S_rrna - 5S ribosomal RNA, at 1,314,907 to 1,315,022
_rrna
HEPCGN_12835: 23S_rrna - 23S ribosomal RNA, at 1,315,116 to 1,318,019
_rrna
Position (kb)
1313
1314
1315 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1312.741 kb on - strand, within srkA at 1312.806 kb on + strand, within srkA at 1312.842 kb on - strand, within srkA at 1312.903 kb on - strand, within srkA at 1312.914 kb on - strand, within srkA at 1312.942 kb on - strand, within srkA at 1312.944 kb on + strand, within srkA at 1313.025 kb on + strand, within srkA at 1313.186 kb on + strand, within srkA at 1313.187 kb on - strand, within srkA at 1313.187 kb on - strand, within srkA at 1313.264 kb on - strand at 1313.264 kb on - strand at 1313.363 kb on + strand at 1313.385 kb on - strand at 1313.385 kb on - strand at 1313.403 kb on - strand, within yihD at 1313.488 kb on - strand, within yihD at 1313.614 kb on + strand, within yihD at 1313.641 kb on + strand at 1313.642 kb on - strand at 1313.644 kb on + strand at 1313.644 kb on + strand at 1313.645 kb on - strand at 1313.783 kb on - strand, within mobA at 1313.840 kb on - strand, within mobA at 1313.898 kb on + strand, within mobA at 1313.967 kb on + strand, within mobA at 1313.968 kb on - strand, within mobA at 1314.020 kb on + strand, within mobA at 1314.021 kb on - strand, within mobA at 1314.021 kb on - strand, within mobA at 1314.021 kb on - strand, within mobA at 1314.036 kb on + strand, within mobA at 1314.148 kb on - strand, within mobA at 1314.148 kb on - strand, within mobA at 1314.190 kb on + strand, within mobA at 1314.191 kb on - strand, within mobA at 1314.191 kb on - strand, within mobA at 1314.256 kb on + strand at 1314.399 kb on + strand, within mobB at 1314.400 kb on - strand, within mobB at 1314.405 kb on + strand, within mobB at 1314.406 kb on - strand, within mobB at 1314.430 kb on - strand, within mobB at 1314.445 kb on - strand, within mobB at 1314.448 kb on + strand, within mobB at 1314.448 kb on + strand, within mobB at 1314.448 kb on + strand, within mobB at 1314.449 kb on - strand, within mobB at 1314.449 kb on - strand, within mobB at 1314.449 kb on - strand, within mobB at 1314.545 kb on + strand, within mobB at 1314.672 kb on - strand, within mobB at 1314.690 kb on + strand, within mobB at 1314.690 kb on + strand, within mobB at 1314.744 kb on + strand, within mobB at 1314.745 kb on - strand, within mobB at 1314.813 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RB23 remove 1,312,741 - srkA HEPCGN_12810 0.44 -0.1 1,312,806 + srkA HEPCGN_12810 0.50 -0.3 1,312,842 - srkA HEPCGN_12810 0.54 -0.2 1,312,903 - srkA HEPCGN_12810 0.60 +0.6 1,312,914 - srkA HEPCGN_12810 0.61 +0.6 1,312,942 - srkA HEPCGN_12810 0.64 -1.3 1,312,944 + srkA HEPCGN_12810 0.64 -0.1 1,313,025 + srkA HEPCGN_12810 0.73 +1.2 1,313,186 + srkA HEPCGN_12810 0.89 -0.0 1,313,187 - srkA HEPCGN_12810 0.89 -0.7 1,313,187 - srkA HEPCGN_12810 0.89 +0.2 1,313,264 - -0.5 1,313,264 - -1.3 1,313,363 + -0.8 1,313,385 - -0.9 1,313,385 - +0.8 1,313,403 - yihD HEPCGN_12815 0.11 -0.9 1,313,488 - yihD HEPCGN_12815 0.43 -0.8 1,313,614 + yihD HEPCGN_12815 0.90 +4.2 1,313,641 + +1.2 1,313,642 - +0.1 1,313,644 + +0.7 1,313,644 + -0.1 1,313,645 - -2.4 1,313,783 - mobA HEPCGN_12820 0.12 +0.2 1,313,840 - mobA HEPCGN_12820 0.22 +0.5 1,313,898 + mobA HEPCGN_12820 0.32 -0.7 1,313,967 + mobA HEPCGN_12820 0.44 +1.8 1,313,968 - mobA HEPCGN_12820 0.44 +1.0 1,314,020 + mobA HEPCGN_12820 0.53 -0.6 1,314,021 - mobA HEPCGN_12820 0.53 +0.4 1,314,021 - mobA HEPCGN_12820 0.53 +1.2 1,314,021 - mobA HEPCGN_12820 0.53 -0.7 1,314,036 + mobA HEPCGN_12820 0.56 +0.4 1,314,148 - mobA HEPCGN_12820 0.75 -0.4 1,314,148 - mobA HEPCGN_12820 0.75 +0.3 1,314,190 + mobA HEPCGN_12820 0.82 -0.8 1,314,191 - mobA HEPCGN_12820 0.82 -0.0 1,314,191 - mobA HEPCGN_12820 0.82 -0.4 1,314,256 + -0.4 1,314,399 + mobB HEPCGN_12825 0.21 +0.2 1,314,400 - mobB HEPCGN_12825 0.21 +0.0 1,314,405 + mobB HEPCGN_12825 0.22 -0.8 1,314,406 - mobB HEPCGN_12825 0.22 -0.0 1,314,430 - mobB HEPCGN_12825 0.27 +0.6 1,314,445 - mobB HEPCGN_12825 0.30 +0.1 1,314,448 + mobB HEPCGN_12825 0.30 +0.1 1,314,448 + mobB HEPCGN_12825 0.30 -0.3 1,314,448 + mobB HEPCGN_12825 0.30 -0.2 1,314,449 - mobB HEPCGN_12825 0.31 +0.2 1,314,449 - mobB HEPCGN_12825 0.31 +0.6 1,314,449 - mobB HEPCGN_12825 0.31 +0.2 1,314,545 + mobB HEPCGN_12825 0.49 -0.1 1,314,672 - mobB HEPCGN_12825 0.74 -0.8 1,314,690 + mobB HEPCGN_12825 0.78 +1.5 1,314,690 + mobB HEPCGN_12825 0.78 -0.2 1,314,744 + mobB HEPCGN_12825 0.88 -0.1 1,314,745 - mobB HEPCGN_12825 0.88 +0.2 1,314,813 - -0.7
Or see this region's nucleotide sequence