Experiment: RB23
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rlmB and rnr are separated by 180 nucleotides rnr and nsrR are separated by 38 nucleotides nsrR and purA are separated by 204 nucleotides
HEPCGN_10445: rlmB - 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB, at 830,053 to 830,784
rlmB
HEPCGN_10450: rnr - ribonuclease R, at 830,965 to 833,406
rnr
HEPCGN_10455: nsrR - nitric oxide-sensing transcriptional repressor NsrR, at 833,445 to 833,870
nsrR
HEPCGN_10460: purA - adenylosuccinate synthase, at 834,075 to 835,373
purA
Position (kb) 830
831
832
833
834 Strain fitness (log2 ratio) -2
-1
0
1 at 829.999 kb on + strand at 830.080 kb on + strand at 830.080 kb on + strand at 830.181 kb on + strand, within rlmB at 830.182 kb on - strand, within rlmB at 830.182 kb on - strand, within rlmB at 830.237 kb on + strand, within rlmB at 830.244 kb on + strand, within rlmB at 830.244 kb on + strand, within rlmB at 830.245 kb on - strand, within rlmB at 830.298 kb on + strand, within rlmB at 830.299 kb on - strand, within rlmB at 830.547 kb on - strand, within rlmB at 830.686 kb on - strand, within rlmB at 830.686 kb on - strand, within rlmB at 830.686 kb on - strand, within rlmB at 830.789 kb on - strand at 830.791 kb on - strand at 830.836 kb on - strand at 830.871 kb on - strand at 830.871 kb on - strand at 831.264 kb on + strand, within rnr at 831.265 kb on - strand, within rnr at 831.362 kb on + strand, within rnr at 831.366 kb on - strand, within rnr at 831.465 kb on + strand, within rnr at 831.474 kb on + strand, within rnr at 831.474 kb on + strand, within rnr at 831.478 kb on + strand, within rnr at 831.479 kb on - strand, within rnr at 831.517 kb on + strand, within rnr at 831.518 kb on - strand, within rnr at 831.518 kb on - strand, within rnr at 831.656 kb on + strand, within rnr at 831.719 kb on + strand, within rnr at 831.838 kb on + strand, within rnr at 831.924 kb on + strand, within rnr at 832.034 kb on - strand, within rnr at 832.068 kb on + strand, within rnr at 832.164 kb on + strand, within rnr at 832.165 kb on - strand, within rnr at 832.165 kb on - strand, within rnr at 832.166 kb on + strand, within rnr at 832.174 kb on - strand, within rnr at 832.174 kb on - strand, within rnr at 832.243 kb on - strand, within rnr at 832.305 kb on - strand, within rnr at 832.350 kb on + strand, within rnr at 832.350 kb on + strand, within rnr at 832.350 kb on + strand, within rnr at 832.351 kb on - strand, within rnr at 832.351 kb on - strand, within rnr at 832.351 kb on - strand, within rnr at 832.351 kb on - strand, within rnr at 832.436 kb on + strand, within rnr at 832.447 kb on - strand, within rnr at 832.447 kb on - strand, within rnr at 832.494 kb on - strand, within rnr at 832.494 kb on - strand, within rnr at 832.497 kb on - strand, within rnr at 832.609 kb on + strand, within rnr at 832.986 kb on + strand, within rnr at 833.007 kb on + strand, within rnr at 833.008 kb on - strand, within rnr at 833.081 kb on + strand, within rnr at 833.082 kb on - strand, within rnr at 833.082 kb on - strand, within rnr at 833.094 kb on + strand, within rnr at 833.145 kb on + strand, within rnr at 833.146 kb on - strand, within rnr at 833.281 kb on + strand at 833.350 kb on - strand at 833.466 kb on - strand at 833.466 kb on - strand at 833.506 kb on + strand, within nsrR at 833.507 kb on - strand, within nsrR at 833.507 kb on - strand, within nsrR at 833.507 kb on - strand, within nsrR at 833.510 kb on + strand, within nsrR at 833.576 kb on + strand, within nsrR at 833.576 kb on + strand, within nsrR at 833.576 kb on + strand, within nsrR at 833.576 kb on + strand, within nsrR at 833.576 kb on + strand, within nsrR at 833.576 kb on + strand, within nsrR at 833.577 kb on - strand, within nsrR at 833.577 kb on - strand, within nsrR at 833.645 kb on + strand, within nsrR at 833.645 kb on + strand, within nsrR at 833.646 kb on - strand, within nsrR at 833.646 kb on - strand, within nsrR at 833.646 kb on - strand, within nsrR at 833.723 kb on + strand, within nsrR at 833.748 kb on - strand, within nsrR at 833.748 kb on - strand, within nsrR at 833.790 kb on - strand, within nsrR at 833.825 kb on - strand, within nsrR at 833.825 kb on - strand, within nsrR at 833.838 kb on + strand at 833.838 kb on + strand at 833.839 kb on - strand at 833.839 kb on - strand at 833.901 kb on + strand at 833.907 kb on + strand at 833.908 kb on - strand at 833.910 kb on - strand at 833.910 kb on - strand at 833.910 kb on - strand at 833.910 kb on - strand at 833.981 kb on + strand at 833.982 kb on - strand at 834.001 kb on + strand at 834.002 kb on - strand at 834.027 kb on - strand at 834.038 kb on - strand at 834.054 kb on + strand at 834.055 kb on - strand at 834.178 kb on + strand at 834.178 kb on + strand at 834.178 kb on + strand at 834.179 kb on - strand at 834.179 kb on - strand at 834.268 kb on - strand, within purA at 834.303 kb on - strand, within purA
Per-strain Table
Position Strand Gene LocusTag Fraction RB23 remove 829,999 + -2.5 830,080 + -0.5 830,080 + -0.4 830,181 + rlmB HEPCGN_10445 0.17 +0.0 830,182 - rlmB HEPCGN_10445 0.18 -0.1 830,182 - rlmB HEPCGN_10445 0.18 +0.5 830,237 + rlmB HEPCGN_10445 0.25 +0.6 830,244 + rlmB HEPCGN_10445 0.26 -1.2 830,244 + rlmB HEPCGN_10445 0.26 +1.4 830,245 - rlmB HEPCGN_10445 0.26 +1.0 830,298 + rlmB HEPCGN_10445 0.33 +0.6 830,299 - rlmB HEPCGN_10445 0.34 -1.1 830,547 - rlmB HEPCGN_10445 0.67 -0.1 830,686 - rlmB HEPCGN_10445 0.86 +0.5 830,686 - rlmB HEPCGN_10445 0.86 -0.1 830,686 - rlmB HEPCGN_10445 0.86 -0.8 830,789 - -1.3 830,791 - +0.8 830,836 - -0.6 830,871 - -0.1 830,871 - +0.5 831,264 + rnr HEPCGN_10450 0.12 -1.5 831,265 - rnr HEPCGN_10450 0.12 +0.5 831,362 + rnr HEPCGN_10450 0.16 +1.2 831,366 - rnr HEPCGN_10450 0.16 +0.3 831,465 + rnr HEPCGN_10450 0.20 +0.5 831,474 + rnr HEPCGN_10450 0.21 +0.1 831,474 + rnr HEPCGN_10450 0.21 -1.2 831,478 + rnr HEPCGN_10450 0.21 +0.2 831,479 - rnr HEPCGN_10450 0.21 +0.3 831,517 + rnr HEPCGN_10450 0.23 +0.5 831,518 - rnr HEPCGN_10450 0.23 +0.5 831,518 - rnr HEPCGN_10450 0.23 -0.1 831,656 + rnr HEPCGN_10450 0.28 +0.1 831,719 + rnr HEPCGN_10450 0.31 +0.1 831,838 + rnr HEPCGN_10450 0.36 +1.2 831,924 + rnr HEPCGN_10450 0.39 +0.6 832,034 - rnr HEPCGN_10450 0.44 -0.0 832,068 + rnr HEPCGN_10450 0.45 +0.3 832,164 + rnr HEPCGN_10450 0.49 +1.2 832,165 - rnr HEPCGN_10450 0.49 -0.5 832,165 - rnr HEPCGN_10450 0.49 +0.1 832,166 + rnr HEPCGN_10450 0.49 +0.7 832,174 - rnr HEPCGN_10450 0.50 -0.1 832,174 - rnr HEPCGN_10450 0.50 -0.1 832,243 - rnr HEPCGN_10450 0.52 +0.4 832,305 - rnr HEPCGN_10450 0.55 -0.4 832,350 + rnr HEPCGN_10450 0.57 -0.4 832,350 + rnr HEPCGN_10450 0.57 +0.8 832,350 + rnr HEPCGN_10450 0.57 -1.1 832,351 - rnr HEPCGN_10450 0.57 -0.6 832,351 - rnr HEPCGN_10450 0.57 -1.4 832,351 - rnr HEPCGN_10450 0.57 +0.0 832,351 - rnr HEPCGN_10450 0.57 -0.8 832,436 + rnr HEPCGN_10450 0.60 -0.0 832,447 - rnr HEPCGN_10450 0.61 -0.0 832,447 - rnr HEPCGN_10450 0.61 -0.4 832,494 - rnr HEPCGN_10450 0.63 -0.6 832,494 - rnr HEPCGN_10450 0.63 -0.0 832,497 - rnr HEPCGN_10450 0.63 +1.5 832,609 + rnr HEPCGN_10450 0.67 +0.0 832,986 + rnr HEPCGN_10450 0.83 -0.2 833,007 + rnr HEPCGN_10450 0.84 -0.2 833,008 - rnr HEPCGN_10450 0.84 +0.2 833,081 + rnr HEPCGN_10450 0.87 +0.3 833,082 - rnr HEPCGN_10450 0.87 -0.0 833,082 - rnr HEPCGN_10450 0.87 -0.0 833,094 + rnr HEPCGN_10450 0.87 -1.6 833,145 + rnr HEPCGN_10450 0.89 +0.1 833,146 - rnr HEPCGN_10450 0.89 -0.1 833,281 + -1.2 833,350 - -0.1 833,466 - -0.4 833,466 - -0.1 833,506 + nsrR HEPCGN_10455 0.14 -0.2 833,507 - nsrR HEPCGN_10455 0.15 -1.2 833,507 - nsrR HEPCGN_10455 0.15 -0.6 833,507 - nsrR HEPCGN_10455 0.15 +0.2 833,510 + nsrR HEPCGN_10455 0.15 +1.2 833,576 + nsrR HEPCGN_10455 0.31 -1.5 833,576 + nsrR HEPCGN_10455 0.31 -1.3 833,576 + nsrR HEPCGN_10455 0.31 -0.0 833,576 + nsrR HEPCGN_10455 0.31 -0.1 833,576 + nsrR HEPCGN_10455 0.31 -0.4 833,576 + nsrR HEPCGN_10455 0.31 +0.6 833,577 - nsrR HEPCGN_10455 0.31 -0.8 833,577 - nsrR HEPCGN_10455 0.31 +0.2 833,645 + nsrR HEPCGN_10455 0.47 -0.2 833,645 + nsrR HEPCGN_10455 0.47 +0.3 833,646 - nsrR HEPCGN_10455 0.47 +0.9 833,646 - nsrR HEPCGN_10455 0.47 -0.5 833,646 - nsrR HEPCGN_10455 0.47 +0.0 833,723 + nsrR HEPCGN_10455 0.65 -0.0 833,748 - nsrR HEPCGN_10455 0.71 +0.5 833,748 - nsrR HEPCGN_10455 0.71 -0.9 833,790 - nsrR HEPCGN_10455 0.81 +0.4 833,825 - nsrR HEPCGN_10455 0.89 -0.8 833,825 - nsrR HEPCGN_10455 0.89 +0.1 833,838 + -0.3 833,838 + +0.6 833,839 - -1.2 833,839 - -0.3 833,901 + -0.6 833,907 + -0.6 833,908 - +0.2 833,910 - +0.2 833,910 - +0.6 833,910 - +0.0 833,910 - +0.6 833,981 + -0.1 833,982 - -1.0 834,001 + -1.7 834,002 - -0.1 834,027 - +0.4 834,038 - +0.8 834,054 + +1.0 834,055 - +0.6 834,178 + -0.3 834,178 + +1.0 834,178 + +0.6 834,179 - -1.2 834,179 - +1.2 834,268 - purA HEPCGN_10460 0.15 +0.6 834,303 - purA HEPCGN_10460 0.18 -0.4
Or see this region's nucleotide sequence