Experiment: RB23
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fliK and fliJ overlap by 4 nucleotides fliJ and fliI are separated by 18 nucleotides fliI and fliH overlap by 1 nucleotides fliH and fliG overlap by 8 nucleotides
HEPCGN_04050: fliK - flagellar hook length control protein FliK, at 709,840 to 710,967
fliK
HEPCGN_04055: fliJ - flagella biosynthesis chaperone FliJ, at 710,964 to 711,407
fliJ
HEPCGN_04060: fliI - flagellum-specific ATP synthase FliI, at 711,426 to 712,799
fliI
HEPCGN_04065: fliH - flagellar assembly protein FliH, at 712,799 to 713,485
fliH
HEPCGN_04070: fliG - flagellar motor switch protein FliG, at 713,478 to 714,473
fliG
Position (kb) 711
712
713 Strain fitness (log2 ratio) -2
-1
0
1
2 at 710.452 kb on - strand, within fliK at 710.598 kb on + strand, within fliK at 711.355 kb on - strand, within fliJ at 711.355 kb on - strand, within fliJ at 711.447 kb on - strand at 711.509 kb on - strand at 711.509 kb on - strand at 711.509 kb on - strand at 711.572 kb on - strand, within fliI at 711.855 kb on - strand, within fliI at 711.917 kb on + strand, within fliI at 712.153 kb on - strand, within fliI at 712.268 kb on + strand, within fliI at 712.344 kb on - strand, within fliI at 712.449 kb on + strand, within fliI at 712.450 kb on - strand, within fliI at 712.517 kb on - strand, within fliI at 712.518 kb on + strand, within fliI at 712.519 kb on - strand, within fliI at 712.519 kb on - strand, within fliI at 712.519 kb on - strand, within fliI at 712.519 kb on - strand, within fliI at 712.605 kb on + strand, within fliI at 712.800 kb on + strand at 712.800 kb on + strand at 712.800 kb on + strand at 712.801 kb on - strand at 712.801 kb on - strand at 712.889 kb on - strand, within fliH at 712.960 kb on + strand, within fliH at 712.960 kb on + strand, within fliH at 713.091 kb on - strand, within fliH at 713.146 kb on + strand, within fliH at 713.164 kb on + strand, within fliH at 713.165 kb on - strand, within fliH at 713.271 kb on + strand, within fliH at 713.320 kb on - strand, within fliH at 713.485 kb on + strand at 713.485 kb on + strand at 713.485 kb on + strand at 713.486 kb on - strand at 713.486 kb on - strand at 713.642 kb on + strand, within fliG at 713.643 kb on - strand, within fliG
Per-strain Table
Position Strand Gene LocusTag Fraction RB23 remove 710,452 - fliK HEPCGN_04050 0.54 -2.3 710,598 + fliK HEPCGN_04050 0.67 -0.1 711,355 - fliJ HEPCGN_04055 0.88 -1.0 711,355 - fliJ HEPCGN_04055 0.88 +0.6 711,447 - -0.7 711,509 - -1.0 711,509 - -0.5 711,509 - -0.1 711,572 - fliI HEPCGN_04060 0.11 -0.2 711,855 - fliI HEPCGN_04060 0.31 -0.6 711,917 + fliI HEPCGN_04060 0.36 -0.5 712,153 - fliI HEPCGN_04060 0.53 -0.6 712,268 + fliI HEPCGN_04060 0.61 +0.6 712,344 - fliI HEPCGN_04060 0.67 -0.4 712,449 + fliI HEPCGN_04060 0.74 +0.2 712,450 - fliI HEPCGN_04060 0.75 +0.4 712,517 - fliI HEPCGN_04060 0.79 -0.6 712,518 + fliI HEPCGN_04060 0.79 +0.4 712,519 - fliI HEPCGN_04060 0.80 +0.2 712,519 - fliI HEPCGN_04060 0.80 -1.0 712,519 - fliI HEPCGN_04060 0.80 +0.3 712,519 - fliI HEPCGN_04060 0.80 -1.2 712,605 + fliI HEPCGN_04060 0.86 +0.6 712,800 + -0.7 712,800 + +0.9 712,800 + +2.5 712,801 - +1.2 712,801 - -1.7 712,889 - fliH HEPCGN_04065 0.13 -0.3 712,960 + fliH HEPCGN_04065 0.23 -1.6 712,960 + fliH HEPCGN_04065 0.23 -0.0 713,091 - fliH HEPCGN_04065 0.43 +0.6 713,146 + fliH HEPCGN_04065 0.51 -0.2 713,164 + fliH HEPCGN_04065 0.53 -0.2 713,165 - fliH HEPCGN_04065 0.53 +0.3 713,271 + fliH HEPCGN_04065 0.69 +1.2 713,320 - fliH HEPCGN_04065 0.76 +0.8 713,485 + +0.1 713,485 + +0.4 713,485 + +1.1 713,486 - -1.0 713,486 - -0.1 713,642 + fliG HEPCGN_04070 0.16 +0.1 713,643 - fliG HEPCGN_04070 0.17 -0.5
Or see this region's nucleotide sequence