Experiment: RB23
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HEPCGN_00085 and HEPCGN_00090 are separated by 62 nucleotides HEPCGN_00090 and rusA are separated by 133 nucleotides rusA and ybcO overlap by 4 nucleotides ybcO and HEPCGN_00105 overlap by 8 nucleotides HEPCGN_00105 and ybcN overlap by 1 nucleotides ybcN and ybcM are separated by 214 nucleotides
HEPCGN_00085: HEPCGN_00085 - antitermination protein, at 5,056 to 5,433
_00085
HEPCGN_00090: HEPCGN_00090 - Orf33, orf151, orf56, orf96, rus, orf45, orf8, orf127, and nmpC genes, at 5,496 to 5,522
_00090
HEPCGN_00095: rusA - crossover junction endodeoxyribonuclease RusA, at 5,656 to 6,018
rusA
HEPCGN_00100: ybcO - Putative nuclease YbcO, at 6,015 to 6,305
ybcO
HEPCGN_00105: HEPCGN_00105 - Prophage protein NinE, at 6,298 to 6,468
_00105
HEPCGN_00110: ybcN - DNA base-flipping protein YbcN, at 6,468 to 6,923
ybcN
HEPCGN_00115: ybcM - Uncharacterized HTH-type transcriptional regulator YbcM, at 7,138 to 7,935
ybcM
Position (kb) 6
7 Strain fitness (log2 ratio) -1
0
1
2 at 5.382 kb on + strand, within HEPCGN_00085 at 5.471 kb on - strand at 5.497 kb on + strand at 5.562 kb on - strand at 5.598 kb on + strand at 5.600 kb on + strand at 5.600 kb on + strand at 5.601 kb on - strand at 5.601 kb on - strand at 5.769 kb on + strand, within rusA at 5.769 kb on + strand, within rusA at 5.770 kb on - strand, within rusA at 5.770 kb on - strand, within rusA at 5.862 kb on + strand, within rusA at 5.862 kb on + strand, within rusA at 5.863 kb on - strand, within rusA at 6.004 kb on - strand at 6.008 kb on + strand at 6.009 kb on - strand at 6.023 kb on + strand at 6.026 kb on + strand at 6.027 kb on - strand at 6.048 kb on - strand, within ybcO at 6.091 kb on + strand, within ybcO at 6.359 kb on + strand, within HEPCGN_00105 at 6.376 kb on + strand, within HEPCGN_00105 at 6.376 kb on + strand, within HEPCGN_00105 at 6.377 kb on - strand, within HEPCGN_00105 at 6.403 kb on - strand, within HEPCGN_00105 at 6.421 kb on + strand, within HEPCGN_00105 at 6.421 kb on + strand, within HEPCGN_00105 at 6.429 kb on - strand, within HEPCGN_00105 at 6.429 kb on - strand, within HEPCGN_00105 at 6.519 kb on - strand, within ybcN at 6.574 kb on + strand, within ybcN at 6.574 kb on + strand, within ybcN at 6.574 kb on + strand, within ybcN at 6.574 kb on + strand, within ybcN at 6.574 kb on + strand, within ybcN at 6.575 kb on - strand, within ybcN at 6.575 kb on - strand, within ybcN at 6.575 kb on - strand, within ybcN at 6.575 kb on - strand, within ybcN at 6.640 kb on - strand, within ybcN at 6.676 kb on + strand, within ybcN at 6.676 kb on + strand, within ybcN at 6.677 kb on - strand, within ybcN at 6.677 kb on - strand, within ybcN at 6.677 kb on - strand, within ybcN at 6.677 kb on - strand, within ybcN at 6.726 kb on - strand, within ybcN at 6.742 kb on + strand, within ybcN at 6.742 kb on + strand, within ybcN at 6.816 kb on - strand, within ybcN at 6.951 kb on - strand at 7.012 kb on - strand at 7.012 kb on - strand at 7.075 kb on + strand at 7.076 kb on - strand at 7.128 kb on + strand at 7.176 kb on + strand at 7.188 kb on + strand at 7.221 kb on + strand, within ybcM at 7.222 kb on - strand, within ybcM at 7.287 kb on + strand, within ybcM at 7.351 kb on + strand, within ybcM at 7.370 kb on + strand, within ybcM at 7.462 kb on - strand, within ybcM
Per-strain Table
Position Strand Gene LocusTag Fraction RB23 remove 5,382 + HEPCGN_00085 0.86 -0.1 5,471 - +0.7 5,497 + -1.1 5,562 - -0.1 5,598 + +0.8 5,600 + -0.8 5,600 + +0.4 5,601 - -1.3 5,601 - +0.5 5,769 + rusA HEPCGN_00095 0.31 +0.0 5,769 + rusA HEPCGN_00095 0.31 -0.7 5,770 - rusA HEPCGN_00095 0.31 -0.6 5,770 - rusA HEPCGN_00095 0.31 +1.8 5,862 + rusA HEPCGN_00095 0.57 -0.5 5,862 + rusA HEPCGN_00095 0.57 +2.2 5,863 - rusA HEPCGN_00095 0.57 +0.5 6,004 - -0.8 6,008 + -0.6 6,009 - -0.4 6,023 + +0.1 6,026 + -0.2 6,027 - -0.1 6,048 - ybcO HEPCGN_00100 0.11 -0.9 6,091 + ybcO HEPCGN_00100 0.26 -0.2 6,359 + HEPCGN_00105 0.36 +0.1 6,376 + HEPCGN_00105 0.46 -0.3 6,376 + HEPCGN_00105 0.46 +1.5 6,377 - HEPCGN_00105 0.46 -0.7 6,403 - HEPCGN_00105 0.61 +1.2 6,421 + HEPCGN_00105 0.72 -1.4 6,421 + HEPCGN_00105 0.72 +0.4 6,429 - HEPCGN_00105 0.77 -0.2 6,429 - HEPCGN_00105 0.77 -0.6 6,519 - ybcN HEPCGN_00110 0.11 -0.4 6,574 + ybcN HEPCGN_00110 0.23 +0.7 6,574 + ybcN HEPCGN_00110 0.23 +0.2 6,574 + ybcN HEPCGN_00110 0.23 -0.0 6,574 + ybcN HEPCGN_00110 0.23 +0.5 6,574 + ybcN HEPCGN_00110 0.23 -0.3 6,575 - ybcN HEPCGN_00110 0.23 -0.2 6,575 - ybcN HEPCGN_00110 0.23 -0.1 6,575 - ybcN HEPCGN_00110 0.23 +0.3 6,575 - ybcN HEPCGN_00110 0.23 -0.8 6,640 - ybcN HEPCGN_00110 0.38 +0.4 6,676 + ybcN HEPCGN_00110 0.46 +0.3 6,676 + ybcN HEPCGN_00110 0.46 +0.0 6,677 - ybcN HEPCGN_00110 0.46 +1.4 6,677 - ybcN HEPCGN_00110 0.46 -0.7 6,677 - ybcN HEPCGN_00110 0.46 +0.0 6,677 - ybcN HEPCGN_00110 0.46 +0.2 6,726 - ybcN HEPCGN_00110 0.57 -0.6 6,742 + ybcN HEPCGN_00110 0.60 +0.1 6,742 + ybcN HEPCGN_00110 0.60 +0.8 6,816 - ybcN HEPCGN_00110 0.76 +0.6 6,951 - -0.1 7,012 - +0.5 7,012 - -1.3 7,075 + -0.3 7,076 - +0.2 7,128 + -0.3 7,176 + -0.8 7,188 + -0.5 7,221 + ybcM HEPCGN_00115 0.10 +1.0 7,222 - ybcM HEPCGN_00115 0.11 +0.2 7,287 + ybcM HEPCGN_00115 0.19 -0.3 7,351 + ybcM HEPCGN_00115 0.27 +0.1 7,370 + ybcM HEPCGN_00115 0.29 -0.9 7,462 - ybcM HEPCGN_00115 0.41 +0.3
Or see this region's nucleotide sequence