Experiment: R2A_PIPES pH 4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt argD and OKGIIK_14095 are separated by 253 nucleotides OKGIIK_14095 and OKGIIK_14100 are separated by 20 nucleotides OKGIIK_14100 and OKGIIK_14105 are separated by 247 nucleotides
OKGIIK_14090: argD - aspartate aminotransferase family protein, at 3,064,117 to 3,065,517
argD
OKGIIK_14095: OKGIIK_14095 - Plasmid maintenance system killer protein, at 3,065,771 to 3,066,049
_14095
OKGIIK_14100: OKGIIK_14100 - HigA family addiction module antitoxin, at 3,066,070 to 3,066,366
_14100
OKGIIK_14105: OKGIIK_14105 - hypothetical protein, at 3,066,614 to 3,067,642
_14105
Position (kb)
3065
3066
3067 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3064.810 kb on + strand, within argD at 3064.810 kb on + strand, within argD at 3064.810 kb on + strand, within argD at 3064.811 kb on - strand, within argD at 3064.811 kb on - strand, within argD at 3064.811 kb on - strand, within argD at 3064.841 kb on - strand, within argD at 3064.841 kb on - strand, within argD at 3065.156 kb on - strand, within argD at 3065.168 kb on - strand, within argD at 3065.410 kb on + strand at 3065.410 kb on + strand at 3065.410 kb on + strand at 3065.411 kb on - strand at 3065.411 kb on - strand at 3065.411 kb on - strand at 3065.411 kb on - strand at 3065.531 kb on - strand at 3065.531 kb on - strand at 3065.744 kb on + strand at 3065.759 kb on + strand at 3065.759 kb on + strand at 3065.759 kb on + strand at 3065.764 kb on + strand at 3065.776 kb on - strand at 3065.776 kb on - strand at 3065.873 kb on + strand, within OKGIIK_14095 at 3065.873 kb on + strand, within OKGIIK_14095 at 3065.874 kb on - strand, within OKGIIK_14095 at 3065.874 kb on - strand, within OKGIIK_14095 at 3065.874 kb on - strand, within OKGIIK_14095 at 3065.874 kb on - strand, within OKGIIK_14095 at 3065.892 kb on + strand, within OKGIIK_14095 at 3066.041 kb on + strand at 3066.042 kb on - strand at 3066.042 kb on - strand at 3066.345 kb on + strand at 3066.345 kb on + strand at 3066.345 kb on + strand at 3066.392 kb on + strand at 3066.393 kb on - strand at 3066.393 kb on - strand at 3066.393 kb on - strand at 3066.393 kb on - strand at 3066.393 kb on - strand at 3066.419 kb on + strand at 3066.419 kb on + strand at 3066.419 kb on + strand at 3066.419 kb on + strand at 3066.420 kb on - strand at 3066.420 kb on - strand at 3066.420 kb on - strand at 3066.420 kb on - strand at 3066.420 kb on - strand at 3066.420 kb on - strand at 3066.420 kb on - strand at 3066.512 kb on + strand at 3066.512 kb on + strand at 3066.512 kb on + strand at 3066.512 kb on + strand at 3066.513 kb on - strand at 3066.513 kb on - strand at 3066.513 kb on - strand at 3066.513 kb on - strand at 3066.513 kb on - strand at 3066.513 kb on - strand at 3066.576 kb on + strand at 3066.576 kb on + strand at 3066.576 kb on + strand at 3066.576 kb on + strand at 3066.576 kb on + strand at 3066.576 kb on + strand at 3066.577 kb on - strand at 3066.577 kb on - strand at 3066.577 kb on - strand at 3066.577 kb on - strand at 3066.577 kb on - strand at 3066.577 kb on - strand at 3066.628 kb on + strand at 3066.628 kb on + strand at 3066.628 kb on + strand at 3066.628 kb on + strand at 3066.628 kb on + strand at 3066.628 kb on + strand at 3066.628 kb on + strand at 3066.628 kb on + strand at 3066.628 kb on + strand at 3066.628 kb on + strand at 3066.629 kb on - strand at 3066.629 kb on - strand at 3066.629 kb on - strand at 3066.629 kb on - strand at 3066.629 kb on - strand at 3066.629 kb on - strand at 3066.629 kb on - strand at 3066.629 kb on - strand at 3066.629 kb on - strand at 3066.629 kb on - strand at 3066.629 kb on - strand at 3066.629 kb on - strand at 3066.629 kb on - strand at 3066.629 kb on - strand at 3066.632 kb on + strand at 3066.632 kb on + strand at 3066.632 kb on + strand at 3066.632 kb on + strand at 3066.632 kb on + strand at 3066.632 kb on + strand at 3066.632 kb on + strand at 3066.632 kb on + strand at 3066.632 kb on + strand at 3066.632 kb on + strand at 3066.632 kb on + strand at 3066.632 kb on + strand at 3066.632 kb on + strand at 3066.632 kb on + strand at 3066.632 kb on + strand at 3066.632 kb on + strand at 3066.632 kb on + strand at 3066.632 kb on + strand at 3066.632 kb on + strand at 3066.632 kb on + strand at 3066.632 kb on + strand at 3066.632 kb on + strand at 3066.632 kb on + strand at 3066.632 kb on + strand at 3066.632 kb on + strand at 3066.632 kb on + strand at 3066.633 kb on - strand at 3066.633 kb on - strand at 3066.633 kb on - strand at 3066.633 kb on - strand at 3066.633 kb on - strand at 3066.633 kb on - strand at 3066.633 kb on - strand at 3066.633 kb on - strand at 3066.633 kb on - strand at 3066.633 kb on - strand at 3066.633 kb on - strand at 3066.633 kb on - strand at 3066.633 kb on - strand at 3066.633 kb on - strand at 3066.633 kb on - strand at 3066.633 kb on - strand at 3066.691 kb on + strand at 3066.691 kb on + strand at 3066.692 kb on - strand at 3066.692 kb on - strand at 3066.771 kb on + strand, within OKGIIK_14105 at 3066.772 kb on - strand, within OKGIIK_14105 at 3066.772 kb on - strand, within OKGIIK_14105 at 3066.850 kb on + strand, within OKGIIK_14105 at 3066.850 kb on + strand, within OKGIIK_14105 at 3066.850 kb on + strand, within OKGIIK_14105 at 3066.850 kb on + strand, within OKGIIK_14105 at 3066.850 kb on + strand, within OKGIIK_14105 at 3066.850 kb on + strand, within OKGIIK_14105 at 3066.851 kb on - strand, within OKGIIK_14105 at 3066.851 kb on - strand, within OKGIIK_14105 at 3066.851 kb on - strand, within OKGIIK_14105 at 3066.851 kb on - strand, within OKGIIK_14105 at 3066.851 kb on - strand, within OKGIIK_14105 at 3066.851 kb on - strand, within OKGIIK_14105 at 3066.871 kb on - strand, within OKGIIK_14105 at 3066.900 kb on + strand, within OKGIIK_14105 at 3066.992 kb on - strand, within OKGIIK_14105
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES pH 4 remove 3,064,810 + argD OKGIIK_14090 0.49 -2.3 3,064,810 + argD OKGIIK_14090 0.49 +1.3 3,064,810 + argD OKGIIK_14090 0.49 -0.7 3,064,811 - argD OKGIIK_14090 0.50 -0.8 3,064,811 - argD OKGIIK_14090 0.50 -2.0 3,064,811 - argD OKGIIK_14090 0.50 +0.1 3,064,841 - argD OKGIIK_14090 0.52 +1.9 3,064,841 - argD OKGIIK_14090 0.52 -0.1 3,065,156 - argD OKGIIK_14090 0.74 -2.8 3,065,168 - argD OKGIIK_14090 0.75 +0.3 3,065,410 + -0.8 3,065,410 + +0.3 3,065,410 + -2.2 3,065,411 - -1.5 3,065,411 - -0.9 3,065,411 - -1.1 3,065,411 - -0.1 3,065,531 - +1.7 3,065,531 - -0.5 3,065,744 + -1.1 3,065,759 + -0.3 3,065,759 + +0.5 3,065,759 + +0.9 3,065,764 + -0.3 3,065,776 - -1.1 3,065,776 - -0.5 3,065,873 + OKGIIK_14095 0.37 +0.3 3,065,873 + OKGIIK_14095 0.37 -0.1 3,065,874 - OKGIIK_14095 0.37 -1.5 3,065,874 - OKGIIK_14095 0.37 -1.3 3,065,874 - OKGIIK_14095 0.37 -0.3 3,065,874 - OKGIIK_14095 0.37 +0.2 3,065,892 + OKGIIK_14095 0.43 +0.6 3,066,041 + +0.5 3,066,042 - +0.5 3,066,042 - -0.1 3,066,345 + -0.1 3,066,345 + -0.9 3,066,345 + -1.1 3,066,392 + +1.3 3,066,393 - +1.1 3,066,393 - -0.2 3,066,393 - +0.8 3,066,393 - +0.5 3,066,393 - -0.5 3,066,419 + +0.0 3,066,419 + -0.4 3,066,419 + -1.1 3,066,419 + -2.1 3,066,420 - -0.7 3,066,420 - -0.1 3,066,420 - +1.3 3,066,420 - +0.9 3,066,420 - -0.1 3,066,420 - +0.8 3,066,420 - +0.2 3,066,512 + +0.3 3,066,512 + -1.1 3,066,512 + -2.3 3,066,512 + +0.1 3,066,513 - -1.9 3,066,513 - +1.3 3,066,513 - -0.5 3,066,513 - +1.3 3,066,513 - -0.9 3,066,513 - +0.3 3,066,576 + -3.1 3,066,576 + -0.3 3,066,576 + -1.5 3,066,576 + -0.2 3,066,576 + -0.1 3,066,576 + +0.9 3,066,577 - -2.8 3,066,577 - -1.8 3,066,577 - -1.2 3,066,577 - -1.3 3,066,577 - -1.2 3,066,577 - -0.1 3,066,628 + +0.8 3,066,628 + -0.7 3,066,628 + +1.5 3,066,628 + +0.9 3,066,628 + +1.6 3,066,628 + -0.5 3,066,628 + -0.7 3,066,628 + -1.1 3,066,628 + -0.8 3,066,628 + -0.4 3,066,629 - +0.2 3,066,629 - +1.4 3,066,629 - +0.4 3,066,629 - -0.4 3,066,629 - -0.3 3,066,629 - -0.3 3,066,629 - +0.5 3,066,629 - -1.3 3,066,629 - -0.3 3,066,629 - -0.9 3,066,629 - -0.6 3,066,629 - +0.3 3,066,629 - -1.6 3,066,629 - +0.3 3,066,632 + -1.2 3,066,632 + +1.5 3,066,632 + -2.2 3,066,632 + -0.9 3,066,632 + -0.1 3,066,632 + -0.1 3,066,632 + +0.4 3,066,632 + -0.7 3,066,632 + +1.3 3,066,632 + -1.2 3,066,632 + -0.7 3,066,632 + -0.8 3,066,632 + +0.8 3,066,632 + +0.4 3,066,632 + -1.3 3,066,632 + +1.1 3,066,632 + -0.1 3,066,632 + +2.5 3,066,632 + -0.1 3,066,632 + +1.1 3,066,632 + -2.5 3,066,632 + +0.8 3,066,632 + +0.5 3,066,632 + -0.7 3,066,632 + +1.0 3,066,632 + -0.4 3,066,633 - -1.9 3,066,633 - +0.5 3,066,633 - -0.7 3,066,633 - -1.6 3,066,633 - +0.0 3,066,633 - +0.5 3,066,633 - +0.5 3,066,633 - -0.9 3,066,633 - -0.7 3,066,633 - -1.8 3,066,633 - -0.3 3,066,633 - -0.7 3,066,633 - -0.4 3,066,633 - -1.3 3,066,633 - -2.0 3,066,633 - -1.8 3,066,691 + +0.5 3,066,691 + -0.9 3,066,692 - -0.3 3,066,692 - -0.1 3,066,771 + OKGIIK_14105 0.15 -0.9 3,066,772 - OKGIIK_14105 0.15 +0.3 3,066,772 - OKGIIK_14105 0.15 +0.0 3,066,850 + OKGIIK_14105 0.23 +0.9 3,066,850 + OKGIIK_14105 0.23 -1.2 3,066,850 + OKGIIK_14105 0.23 +1.3 3,066,850 + OKGIIK_14105 0.23 -0.4 3,066,850 + OKGIIK_14105 0.23 -1.6 3,066,850 + OKGIIK_14105 0.23 -1.2 3,066,851 - OKGIIK_14105 0.23 +0.7 3,066,851 - OKGIIK_14105 0.23 +1.7 3,066,851 - OKGIIK_14105 0.23 -0.3 3,066,851 - OKGIIK_14105 0.23 +0.3 3,066,851 - OKGIIK_14105 0.23 -0.9 3,066,851 - OKGIIK_14105 0.23 +0.9 3,066,871 - OKGIIK_14105 0.25 +0.4 3,066,900 + OKGIIK_14105 0.28 -0.7 3,066,992 - OKGIIK_14105 0.37 +1.2
Or see this region's nucleotide sequence