Experiment: R2A_PIPES pH 4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thrC and leuA are separated by 473 nucleotides leuA and ilvE overlap by 4 nucleotides ilvE and leuC overlap by 4 nucleotides
OKGIIK_13570: thrC - threonine synthase, at 2,940,431 to 2,941,747
thrC
OKGIIK_13575: leuA - 2-isopropylmalate synthase, at 2,942,221 to 2,943,876
leuA
OKGIIK_13580: ilvE - branched-chain amino acid transaminase, at 2,943,873 to 2,944,874
ilvE
OKGIIK_13585: leuC - 3-isopropylmalate dehydratase large subunit, at 2,944,871 to 2,946,283
leuC
Position (kb)
2942
2943
2944 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2941.400 kb on + strand, within thrC at 2941.746 kb on - strand at 2941.746 kb on - strand at 2941.746 kb on - strand at 2941.746 kb on - strand at 2941.746 kb on - strand at 2941.746 kb on - strand at 2941.780 kb on + strand at 2941.780 kb on + strand at 2941.780 kb on + strand at 2941.781 kb on - strand at 2941.781 kb on - strand at 2941.781 kb on - strand at 2941.781 kb on - strand at 2941.782 kb on + strand at 2941.782 kb on + strand at 2941.782 kb on + strand at 2941.782 kb on + strand at 2941.782 kb on + strand at 2941.782 kb on + strand at 2941.782 kb on + strand at 2941.783 kb on - strand at 2941.783 kb on - strand at 2941.783 kb on - strand at 2941.783 kb on - strand at 2941.812 kb on + strand at 2941.812 kb on + strand at 2941.812 kb on + strand at 2941.960 kb on - strand at 2941.960 kb on - strand at 2941.960 kb on - strand at 2942.061 kb on + strand at 2942.061 kb on + strand at 2942.061 kb on + strand at 2942.061 kb on + strand at 2942.061 kb on + strand at 2942.061 kb on + strand at 2942.061 kb on + strand at 2942.062 kb on - strand at 2942.062 kb on - strand at 2942.062 kb on - strand at 2942.062 kb on - strand at 2942.062 kb on - strand at 2942.062 kb on - strand at 2942.062 kb on - strand at 2942.065 kb on - strand at 2942.065 kb on - strand at 2942.065 kb on - strand at 2942.073 kb on - strand at 2942.073 kb on - strand at 2942.073 kb on - strand at 2942.078 kb on + strand at 2942.079 kb on - strand at 2942.085 kb on + strand at 2942.085 kb on + strand at 2942.085 kb on + strand at 2942.086 kb on - strand at 2942.086 kb on - strand at 2942.086 kb on - strand at 2942.086 kb on - strand at 2942.086 kb on - strand at 2942.086 kb on - strand at 2942.088 kb on + strand at 2942.089 kb on - strand at 2942.089 kb on - strand at 2942.126 kb on - strand at 2942.126 kb on - strand at 2942.130 kb on - strand at 2942.130 kb on - strand at 2942.130 kb on - strand at 2942.267 kb on + strand at 2942.268 kb on - strand at 2942.788 kb on + strand, within leuA at 2942.788 kb on + strand, within leuA at 2942.789 kb on - strand, within leuA at 2942.824 kb on + strand, within leuA at 2942.824 kb on + strand, within leuA at 2942.825 kb on - strand, within leuA at 2942.825 kb on - strand, within leuA at 2942.825 kb on - strand, within leuA at 2943.067 kb on + strand, within leuA at 2943.067 kb on + strand, within leuA at 2943.199 kb on + strand, within leuA at 2943.199 kb on + strand, within leuA at 2943.200 kb on - strand, within leuA at 2943.200 kb on - strand, within leuA at 2943.200 kb on - strand, within leuA at 2943.200 kb on - strand, within leuA at 2943.200 kb on - strand, within leuA at 2943.200 kb on - strand, within leuA at 2943.200 kb on - strand, within leuA at 2943.958 kb on - strand at 2943.958 kb on - strand at 2943.958 kb on - strand at 2943.990 kb on + strand, within ilvE at 2943.990 kb on + strand, within ilvE at 2943.990 kb on + strand, within ilvE at 2943.990 kb on + strand, within ilvE at 2943.990 kb on + strand, within ilvE at 2943.990 kb on + strand, within ilvE at 2943.991 kb on - strand, within ilvE at 2943.991 kb on - strand, within ilvE at 2943.991 kb on - strand, within ilvE at 2943.991 kb on - strand, within ilvE at 2943.991 kb on - strand, within ilvE at 2944.014 kb on + strand, within ilvE at 2944.014 kb on + strand, within ilvE at 2944.015 kb on - strand, within ilvE at 2944.015 kb on - strand, within ilvE at 2944.015 kb on - strand, within ilvE at 2944.149 kb on + strand, within ilvE at 2944.149 kb on + strand, within ilvE at 2944.149 kb on + strand, within ilvE at 2944.149 kb on + strand, within ilvE at 2944.149 kb on + strand, within ilvE at 2944.149 kb on + strand, within ilvE at 2944.149 kb on + strand, within ilvE at 2944.150 kb on - strand, within ilvE at 2944.150 kb on - strand, within ilvE at 2944.150 kb on - strand, within ilvE at 2944.150 kb on - strand, within ilvE at 2944.150 kb on - strand, within ilvE at 2944.150 kb on - strand, within ilvE at 2944.150 kb on - strand, within ilvE at 2944.150 kb on - strand, within ilvE at 2944.150 kb on - strand, within ilvE at 2944.251 kb on + strand, within ilvE at 2944.251 kb on + strand, within ilvE at 2944.251 kb on + strand, within ilvE at 2944.251 kb on + strand, within ilvE at 2944.251 kb on + strand, within ilvE at 2944.251 kb on + strand, within ilvE at 2944.252 kb on - strand, within ilvE at 2944.252 kb on - strand, within ilvE at 2944.353 kb on + strand, within ilvE at 2944.353 kb on + strand, within ilvE at 2944.353 kb on + strand, within ilvE at 2944.353 kb on + strand, within ilvE at 2944.353 kb on + strand, within ilvE at 2944.353 kb on + strand, within ilvE at 2944.354 kb on - strand, within ilvE at 2944.354 kb on - strand, within ilvE at 2944.354 kb on - strand, within ilvE at 2944.354 kb on - strand, within ilvE at 2944.354 kb on - strand, within ilvE at 2944.354 kb on - strand, within ilvE at 2944.354 kb on - strand, within ilvE at 2944.354 kb on - strand, within ilvE at 2944.602 kb on + strand, within ilvE at 2944.603 kb on - strand, within ilvE
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES pH 4 remove 2,941,400 + thrC OKGIIK_13570 0.74 -1.1 2,941,746 - +1.0 2,941,746 - -2.1 2,941,746 - -0.1 2,941,746 - +0.4 2,941,746 - -0.2 2,941,746 - -0.1 2,941,780 + -0.6 2,941,780 + -0.6 2,941,780 + -1.1 2,941,781 - -1.5 2,941,781 - +0.3 2,941,781 - +0.1 2,941,781 - -1.8 2,941,782 + -1.6 2,941,782 + -1.7 2,941,782 + -0.6 2,941,782 + -0.3 2,941,782 + +0.2 2,941,782 + +1.1 2,941,782 + -0.2 2,941,783 - -0.6 2,941,783 - +0.2 2,941,783 - -1.2 2,941,783 - +0.2 2,941,812 + -0.3 2,941,812 + +0.2 2,941,812 + -0.3 2,941,960 - +0.2 2,941,960 - -0.6 2,941,960 - -0.1 2,942,061 + +0.7 2,942,061 + -0.5 2,942,061 + -0.1 2,942,061 + -2.0 2,942,061 + -0.9 2,942,061 + +0.5 2,942,061 + +0.1 2,942,062 - +0.4 2,942,062 - +1.2 2,942,062 - +0.2 2,942,062 - +1.2 2,942,062 - +0.2 2,942,062 - -0.9 2,942,062 - -0.9 2,942,065 - +0.9 2,942,065 - +0.9 2,942,065 - -0.1 2,942,073 - -0.9 2,942,073 - -0.5 2,942,073 - -1.3 2,942,078 + +0.2 2,942,079 - +1.2 2,942,085 + +1.0 2,942,085 + -0.3 2,942,085 + -0.7 2,942,086 - +0.2 2,942,086 - -0.1 2,942,086 - -1.5 2,942,086 - -0.6 2,942,086 - +1.0 2,942,086 - -0.7 2,942,088 + +0.3 2,942,089 - -0.1 2,942,089 - +0.3 2,942,126 - -0.3 2,942,126 - +0.8 2,942,130 - -1.6 2,942,130 - +0.9 2,942,130 - +0.4 2,942,267 + +0.4 2,942,268 - -0.9 2,942,788 + leuA OKGIIK_13575 0.34 -0.3 2,942,788 + leuA OKGIIK_13575 0.34 +0.8 2,942,789 - leuA OKGIIK_13575 0.34 -1.7 2,942,824 + leuA OKGIIK_13575 0.36 -1.0 2,942,824 + leuA OKGIIK_13575 0.36 +0.3 2,942,825 - leuA OKGIIK_13575 0.36 +0.6 2,942,825 - leuA OKGIIK_13575 0.36 -0.2 2,942,825 - leuA OKGIIK_13575 0.36 +0.2 2,943,067 + leuA OKGIIK_13575 0.51 +0.7 2,943,067 + leuA OKGIIK_13575 0.51 +0.4 2,943,199 + leuA OKGIIK_13575 0.59 +1.2 2,943,199 + leuA OKGIIK_13575 0.59 +0.2 2,943,200 - leuA OKGIIK_13575 0.59 -0.5 2,943,200 - leuA OKGIIK_13575 0.59 +1.6 2,943,200 - leuA OKGIIK_13575 0.59 -1.3 2,943,200 - leuA OKGIIK_13575 0.59 -0.1 2,943,200 - leuA OKGIIK_13575 0.59 -0.8 2,943,200 - leuA OKGIIK_13575 0.59 -0.7 2,943,200 - leuA OKGIIK_13575 0.59 +2.6 2,943,958 - -0.1 2,943,958 - -0.9 2,943,958 - +0.2 2,943,990 + ilvE OKGIIK_13580 0.12 +1.0 2,943,990 + ilvE OKGIIK_13580 0.12 -0.8 2,943,990 + ilvE OKGIIK_13580 0.12 -0.9 2,943,990 + ilvE OKGIIK_13580 0.12 -0.7 2,943,990 + ilvE OKGIIK_13580 0.12 +0.2 2,943,990 + ilvE OKGIIK_13580 0.12 -1.1 2,943,991 - ilvE OKGIIK_13580 0.12 -1.3 2,943,991 - ilvE OKGIIK_13580 0.12 -1.9 2,943,991 - ilvE OKGIIK_13580 0.12 +1.8 2,943,991 - ilvE OKGIIK_13580 0.12 +1.8 2,943,991 - ilvE OKGIIK_13580 0.12 +0.0 2,944,014 + ilvE OKGIIK_13580 0.14 -1.8 2,944,014 + ilvE OKGIIK_13580 0.14 -1.5 2,944,015 - ilvE OKGIIK_13580 0.14 -1.8 2,944,015 - ilvE OKGIIK_13580 0.14 -0.3 2,944,015 - ilvE OKGIIK_13580 0.14 -2.1 2,944,149 + ilvE OKGIIK_13580 0.28 -0.5 2,944,149 + ilvE OKGIIK_13580 0.28 -0.3 2,944,149 + ilvE OKGIIK_13580 0.28 +0.9 2,944,149 + ilvE OKGIIK_13580 0.28 -0.9 2,944,149 + ilvE OKGIIK_13580 0.28 +1.1 2,944,149 + ilvE OKGIIK_13580 0.28 -0.1 2,944,149 + ilvE OKGIIK_13580 0.28 -0.6 2,944,150 - ilvE OKGIIK_13580 0.28 -2.1 2,944,150 - ilvE OKGIIK_13580 0.28 +0.5 2,944,150 - ilvE OKGIIK_13580 0.28 -0.8 2,944,150 - ilvE OKGIIK_13580 0.28 +0.2 2,944,150 - ilvE OKGIIK_13580 0.28 -1.2 2,944,150 - ilvE OKGIIK_13580 0.28 -2.4 2,944,150 - ilvE OKGIIK_13580 0.28 -0.3 2,944,150 - ilvE OKGIIK_13580 0.28 -0.2 2,944,150 - ilvE OKGIIK_13580 0.28 -2.9 2,944,251 + ilvE OKGIIK_13580 0.38 +0.3 2,944,251 + ilvE OKGIIK_13580 0.38 -0.1 2,944,251 + ilvE OKGIIK_13580 0.38 -0.4 2,944,251 + ilvE OKGIIK_13580 0.38 -1.1 2,944,251 + ilvE OKGIIK_13580 0.38 -0.1 2,944,251 + ilvE OKGIIK_13580 0.38 -0.3 2,944,252 - ilvE OKGIIK_13580 0.38 -0.2 2,944,252 - ilvE OKGIIK_13580 0.38 +0.2 2,944,353 + ilvE OKGIIK_13580 0.48 +0.8 2,944,353 + ilvE OKGIIK_13580 0.48 -0.3 2,944,353 + ilvE OKGIIK_13580 0.48 +0.2 2,944,353 + ilvE OKGIIK_13580 0.48 +0.1 2,944,353 + ilvE OKGIIK_13580 0.48 +0.4 2,944,353 + ilvE OKGIIK_13580 0.48 -0.9 2,944,354 - ilvE OKGIIK_13580 0.48 +0.2 2,944,354 - ilvE OKGIIK_13580 0.48 +0.7 2,944,354 - ilvE OKGIIK_13580 0.48 +0.5 2,944,354 - ilvE OKGIIK_13580 0.48 +0.0 2,944,354 - ilvE OKGIIK_13580 0.48 -0.1 2,944,354 - ilvE OKGIIK_13580 0.48 -2.3 2,944,354 - ilvE OKGIIK_13580 0.48 -0.3 2,944,354 - ilvE OKGIIK_13580 0.48 -0.1 2,944,602 + ilvE OKGIIK_13580 0.73 -2.3 2,944,603 - ilvE OKGIIK_13580 0.73 -1.3
Or see this region's nucleotide sequence