Experiment: R2A_PIPES pH 4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dgoD and OKGIIK_12515 are separated by 2 nucleotides OKGIIK_12515 and OKGIIK_12520 overlap by 4 nucleotides OKGIIK_12520 and OKGIIK_12525 are separated by 49 nucleotides
OKGIIK_12510: dgoD - galactonate dehydratase, at 2,693,769 to 2,694,917
dgoD
OKGIIK_12515: OKGIIK_12515 - 3-oxoacyl-ACP reductase, at 2,694,920 to 2,695,684
_12515
OKGIIK_12520: OKGIIK_12520 - Gluconolaconase, at 2,695,681 to 2,696,586
_12520
OKGIIK_12525: OKGIIK_12525 - D-galactose transporter GalP, at 2,696,636 to 2,698,027
_12525
Position (kb)
2694
2695
2696 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6
7 at 2693.920 kb on - strand, within dgoD at 2693.970 kb on + strand, within dgoD at 2693.970 kb on + strand, within dgoD at 2693.970 kb on + strand, within dgoD at 2693.971 kb on - strand, within dgoD at 2693.971 kb on - strand, within dgoD at 2693.985 kb on + strand, within dgoD at 2693.986 kb on - strand, within dgoD at 2694.118 kb on - strand, within dgoD at 2694.118 kb on - strand, within dgoD at 2694.118 kb on - strand, within dgoD at 2694.225 kb on + strand, within dgoD at 2694.225 kb on + strand, within dgoD at 2694.226 kb on - strand, within dgoD at 2694.226 kb on - strand, within dgoD at 2694.226 kb on - strand, within dgoD at 2694.226 kb on - strand, within dgoD at 2694.278 kb on - strand, within dgoD at 2694.375 kb on + strand, within dgoD at 2694.376 kb on - strand, within dgoD at 2694.376 kb on - strand, within dgoD at 2694.480 kb on + strand, within dgoD at 2694.480 kb on + strand, within dgoD at 2694.481 kb on - strand, within dgoD at 2694.481 kb on - strand, within dgoD at 2694.489 kb on + strand, within dgoD at 2694.506 kb on - strand, within dgoD at 2694.600 kb on + strand, within dgoD at 2694.600 kb on + strand, within dgoD at 2694.601 kb on - strand, within dgoD at 2694.601 kb on - strand, within dgoD at 2694.601 kb on - strand, within dgoD at 2694.601 kb on - strand, within dgoD at 2694.601 kb on - strand, within dgoD at 2694.601 kb on - strand, within dgoD at 2694.601 kb on - strand, within dgoD at 2694.601 kb on - strand, within dgoD at 2694.601 kb on - strand, within dgoD at 2694.717 kb on + strand, within dgoD at 2694.717 kb on + strand, within dgoD at 2694.717 kb on + strand, within dgoD at 2694.717 kb on + strand, within dgoD at 2694.718 kb on - strand, within dgoD at 2694.744 kb on + strand, within dgoD at 2694.744 kb on + strand, within dgoD at 2694.745 kb on - strand, within dgoD at 2694.745 kb on - strand, within dgoD at 2694.745 kb on - strand, within dgoD at 2694.745 kb on - strand, within dgoD at 2694.745 kb on - strand, within dgoD at 2694.763 kb on + strand, within dgoD at 2694.763 kb on + strand, within dgoD at 2694.763 kb on + strand, within dgoD at 2694.764 kb on - strand, within dgoD at 2694.764 kb on - strand, within dgoD at 2694.764 kb on - strand, within dgoD at 2694.764 kb on - strand, within dgoD at 2694.764 kb on - strand, within dgoD at 2694.772 kb on - strand, within dgoD at 2694.834 kb on + strand at 2694.835 kb on - strand at 2694.835 kb on - strand at 2694.835 kb on - strand at 2694.835 kb on - strand at 2694.835 kb on - strand at 2694.938 kb on + strand at 2694.939 kb on - strand at 2694.939 kb on - strand at 2695.221 kb on + strand, within OKGIIK_12515 at 2695.221 kb on + strand, within OKGIIK_12515 at 2695.221 kb on + strand, within OKGIIK_12515 at 2695.221 kb on + strand, within OKGIIK_12515 at 2695.221 kb on + strand, within OKGIIK_12515 at 2695.221 kb on + strand, within OKGIIK_12515 at 2695.221 kb on + strand, within OKGIIK_12515 at 2695.221 kb on + strand, within OKGIIK_12515 at 2695.222 kb on - strand, within OKGIIK_12515 at 2695.222 kb on - strand, within OKGIIK_12515 at 2695.222 kb on - strand, within OKGIIK_12515 at 2695.222 kb on - strand, within OKGIIK_12515 at 2695.222 kb on - strand, within OKGIIK_12515 at 2695.222 kb on - strand, within OKGIIK_12515 at 2695.222 kb on - strand, within OKGIIK_12515 at 2695.222 kb on - strand, within OKGIIK_12515 at 2695.222 kb on - strand, within OKGIIK_12515 at 2695.222 kb on - strand, within OKGIIK_12515 at 2695.292 kb on + strand, within OKGIIK_12515 at 2695.292 kb on + strand, within OKGIIK_12515 at 2695.292 kb on + strand, within OKGIIK_12515 at 2695.292 kb on + strand, within OKGIIK_12515 at 2695.293 kb on - strand, within OKGIIK_12515 at 2695.293 kb on - strand, within OKGIIK_12515 at 2695.293 kb on - strand, within OKGIIK_12515 at 2695.293 kb on - strand, within OKGIIK_12515 at 2695.293 kb on - strand, within OKGIIK_12515 at 2695.293 kb on - strand, within OKGIIK_12515 at 2695.293 kb on - strand, within OKGIIK_12515 at 2695.293 kb on - strand, within OKGIIK_12515 at 2695.400 kb on + strand, within OKGIIK_12515 at 2695.400 kb on + strand, within OKGIIK_12515 at 2695.400 kb on + strand, within OKGIIK_12515 at 2695.400 kb on + strand, within OKGIIK_12515 at 2695.400 kb on + strand, within OKGIIK_12515 at 2695.400 kb on + strand, within OKGIIK_12515 at 2695.400 kb on + strand, within OKGIIK_12515 at 2695.401 kb on - strand, within OKGIIK_12515 at 2695.401 kb on - strand, within OKGIIK_12515 at 2695.401 kb on - strand, within OKGIIK_12515 at 2695.401 kb on - strand, within OKGIIK_12515 at 2695.401 kb on - strand, within OKGIIK_12515 at 2695.401 kb on - strand, within OKGIIK_12515 at 2695.646 kb on - strand at 2695.680 kb on + strand at 2695.680 kb on + strand at 2695.681 kb on - strand at 2695.681 kb on - strand at 2695.765 kb on + strand at 2695.765 kb on + strand at 2695.765 kb on + strand at 2695.893 kb on - strand, within OKGIIK_12520 at 2695.893 kb on - strand, within OKGIIK_12520 at 2695.893 kb on - strand, within OKGIIK_12520 at 2695.953 kb on + strand, within OKGIIK_12520 at 2695.954 kb on - strand, within OKGIIK_12520 at 2695.954 kb on - strand, within OKGIIK_12520 at 2695.954 kb on - strand, within OKGIIK_12520 at 2696.077 kb on + strand, within OKGIIK_12520 at 2696.077 kb on + strand, within OKGIIK_12520 at 2696.078 kb on - strand, within OKGIIK_12520 at 2696.166 kb on + strand, within OKGIIK_12520 at 2696.166 kb on + strand, within OKGIIK_12520 at 2696.166 kb on + strand, within OKGIIK_12520 at 2696.167 kb on - strand, within OKGIIK_12520 at 2696.167 kb on - strand, within OKGIIK_12520 at 2696.167 kb on - strand, within OKGIIK_12520 at 2696.167 kb on - strand, within OKGIIK_12520 at 2696.167 kb on - strand, within OKGIIK_12520 at 2696.167 kb on - strand, within OKGIIK_12520 at 2696.173 kb on + strand, within OKGIIK_12520 at 2696.173 kb on + strand, within OKGIIK_12520 at 2696.173 kb on + strand, within OKGIIK_12520 at 2696.173 kb on + strand, within OKGIIK_12520 at 2696.173 kb on + strand, within OKGIIK_12520 at 2696.173 kb on + strand, within OKGIIK_12520 at 2696.174 kb on - strand, within OKGIIK_12520 at 2696.174 kb on - strand, within OKGIIK_12520 at 2696.174 kb on - strand, within OKGIIK_12520 at 2696.174 kb on - strand, within OKGIIK_12520 at 2696.174 kb on - strand, within OKGIIK_12520 at 2696.176 kb on + strand, within OKGIIK_12520 at 2696.177 kb on - strand, within OKGIIK_12520 at 2696.215 kb on + strand, within OKGIIK_12520 at 2696.215 kb on + strand, within OKGIIK_12520 at 2696.347 kb on + strand, within OKGIIK_12520 at 2696.347 kb on + strand, within OKGIIK_12520 at 2696.347 kb on + strand, within OKGIIK_12520 at 2696.347 kb on + strand, within OKGIIK_12520 at 2696.348 kb on - strand, within OKGIIK_12520 at 2696.348 kb on - strand, within OKGIIK_12520 at 2696.348 kb on - strand, within OKGIIK_12520 at 2696.348 kb on - strand, within OKGIIK_12520 at 2696.440 kb on + strand, within OKGIIK_12520 at 2696.440 kb on + strand, within OKGIIK_12520 at 2696.440 kb on + strand, within OKGIIK_12520 at 2696.440 kb on + strand, within OKGIIK_12520 at 2696.441 kb on - strand, within OKGIIK_12520 at 2696.441 kb on - strand, within OKGIIK_12520 at 2696.441 kb on - strand, within OKGIIK_12520 at 2696.441 kb on - strand, within OKGIIK_12520 at 2696.441 kb on - strand, within OKGIIK_12520 at 2696.441 kb on - strand, within OKGIIK_12520 at 2696.441 kb on - strand, within OKGIIK_12520 at 2696.584 kb on + strand at 2696.584 kb on + strand at 2696.584 kb on + strand at 2696.584 kb on + strand at 2696.584 kb on + strand at 2696.584 kb on + strand at 2696.584 kb on + strand at 2696.585 kb on - strand at 2696.585 kb on - strand at 2696.585 kb on - strand at 2696.585 kb on - strand at 2696.610 kb on + strand at 2696.617 kb on + strand at 2696.635 kb on + strand at 2696.635 kb on + strand at 2696.636 kb on - strand at 2696.636 kb on - strand at 2696.636 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES pH 4 remove 2,693,920 - dgoD OKGIIK_12510 0.13 -0.7 2,693,970 + dgoD OKGIIK_12510 0.17 -0.7 2,693,970 + dgoD OKGIIK_12510 0.17 +0.7 2,693,970 + dgoD OKGIIK_12510 0.17 -0.7 2,693,971 - dgoD OKGIIK_12510 0.18 -0.3 2,693,971 - dgoD OKGIIK_12510 0.18 +1.5 2,693,985 + dgoD OKGIIK_12510 0.19 +0.2 2,693,986 - dgoD OKGIIK_12510 0.19 -1.8 2,694,118 - dgoD OKGIIK_12510 0.30 +0.1 2,694,118 - dgoD OKGIIK_12510 0.30 +0.0 2,694,118 - dgoD OKGIIK_12510 0.30 -0.8 2,694,225 + dgoD OKGIIK_12510 0.40 +1.5 2,694,225 + dgoD OKGIIK_12510 0.40 -1.0 2,694,226 - dgoD OKGIIK_12510 0.40 -2.5 2,694,226 - dgoD OKGIIK_12510 0.40 -2.4 2,694,226 - dgoD OKGIIK_12510 0.40 +0.1 2,694,226 - dgoD OKGIIK_12510 0.40 +1.9 2,694,278 - dgoD OKGIIK_12510 0.44 -0.1 2,694,375 + dgoD OKGIIK_12510 0.53 +0.3 2,694,376 - dgoD OKGIIK_12510 0.53 -0.7 2,694,376 - dgoD OKGIIK_12510 0.53 +0.1 2,694,480 + dgoD OKGIIK_12510 0.62 +0.6 2,694,480 + dgoD OKGIIK_12510 0.62 +1.5 2,694,481 - dgoD OKGIIK_12510 0.62 +0.5 2,694,481 - dgoD OKGIIK_12510 0.62 -0.4 2,694,489 + dgoD OKGIIK_12510 0.63 +0.3 2,694,506 - dgoD OKGIIK_12510 0.64 +1.8 2,694,600 + dgoD OKGIIK_12510 0.72 +0.4 2,694,600 + dgoD OKGIIK_12510 0.72 -1.3 2,694,601 - dgoD OKGIIK_12510 0.72 +0.3 2,694,601 - dgoD OKGIIK_12510 0.72 +0.1 2,694,601 - dgoD OKGIIK_12510 0.72 -1.1 2,694,601 - dgoD OKGIIK_12510 0.72 +0.3 2,694,601 - dgoD OKGIIK_12510 0.72 -0.1 2,694,601 - dgoD OKGIIK_12510 0.72 -0.1 2,694,601 - dgoD OKGIIK_12510 0.72 -1.1 2,694,601 - dgoD OKGIIK_12510 0.72 +1.8 2,694,601 - dgoD OKGIIK_12510 0.72 +0.5 2,694,717 + dgoD OKGIIK_12510 0.83 -0.9 2,694,717 + dgoD OKGIIK_12510 0.83 +1.5 2,694,717 + dgoD OKGIIK_12510 0.83 -0.2 2,694,717 + dgoD OKGIIK_12510 0.83 +0.3 2,694,718 - dgoD OKGIIK_12510 0.83 -0.4 2,694,744 + dgoD OKGIIK_12510 0.85 +0.1 2,694,744 + dgoD OKGIIK_12510 0.85 +1.5 2,694,745 - dgoD OKGIIK_12510 0.85 -1.8 2,694,745 - dgoD OKGIIK_12510 0.85 -0.3 2,694,745 - dgoD OKGIIK_12510 0.85 +0.9 2,694,745 - dgoD OKGIIK_12510 0.85 +1.3 2,694,745 - dgoD OKGIIK_12510 0.85 -1.6 2,694,763 + dgoD OKGIIK_12510 0.87 +0.3 2,694,763 + dgoD OKGIIK_12510 0.87 +1.7 2,694,763 + dgoD OKGIIK_12510 0.87 -1.3 2,694,764 - dgoD OKGIIK_12510 0.87 -0.3 2,694,764 - dgoD OKGIIK_12510 0.87 -2.3 2,694,764 - dgoD OKGIIK_12510 0.87 +0.7 2,694,764 - dgoD OKGIIK_12510 0.87 +1.3 2,694,764 - dgoD OKGIIK_12510 0.87 -0.8 2,694,772 - dgoD OKGIIK_12510 0.87 +0.1 2,694,834 + -0.3 2,694,835 - +1.5 2,694,835 - +0.9 2,694,835 - +0.4 2,694,835 - +0.1 2,694,835 - -0.7 2,694,938 + +0.8 2,694,939 - +1.0 2,694,939 - -0.7 2,695,221 + OKGIIK_12515 0.39 +2.1 2,695,221 + OKGIIK_12515 0.39 +0.3 2,695,221 + OKGIIK_12515 0.39 -1.1 2,695,221 + OKGIIK_12515 0.39 -0.2 2,695,221 + OKGIIK_12515 0.39 -0.1 2,695,221 + OKGIIK_12515 0.39 +0.7 2,695,221 + OKGIIK_12515 0.39 -0.5 2,695,221 + OKGIIK_12515 0.39 -0.4 2,695,222 - OKGIIK_12515 0.39 +0.3 2,695,222 - OKGIIK_12515 0.39 -0.5 2,695,222 - OKGIIK_12515 0.39 -1.1 2,695,222 - OKGIIK_12515 0.39 -0.3 2,695,222 - OKGIIK_12515 0.39 -1.0 2,695,222 - OKGIIK_12515 0.39 +0.7 2,695,222 - OKGIIK_12515 0.39 +0.5 2,695,222 - OKGIIK_12515 0.39 +0.1 2,695,222 - OKGIIK_12515 0.39 +1.9 2,695,222 - OKGIIK_12515 0.39 -1.2 2,695,292 + OKGIIK_12515 0.49 +0.9 2,695,292 + OKGIIK_12515 0.49 -2.2 2,695,292 + OKGIIK_12515 0.49 +0.7 2,695,292 + OKGIIK_12515 0.49 -0.5 2,695,293 - OKGIIK_12515 0.49 -0.1 2,695,293 - OKGIIK_12515 0.49 +1.0 2,695,293 - OKGIIK_12515 0.49 +0.5 2,695,293 - OKGIIK_12515 0.49 -1.3 2,695,293 - OKGIIK_12515 0.49 +0.4 2,695,293 - OKGIIK_12515 0.49 -0.5 2,695,293 - OKGIIK_12515 0.49 -1.2 2,695,293 - OKGIIK_12515 0.49 +1.6 2,695,400 + OKGIIK_12515 0.63 +1.0 2,695,400 + OKGIIK_12515 0.63 +0.8 2,695,400 + OKGIIK_12515 0.63 +7.5 2,695,400 + OKGIIK_12515 0.63 +0.0 2,695,400 + OKGIIK_12515 0.63 +0.3 2,695,400 + OKGIIK_12515 0.63 -0.3 2,695,400 + OKGIIK_12515 0.63 +1.3 2,695,401 - OKGIIK_12515 0.63 +0.1 2,695,401 - OKGIIK_12515 0.63 -1.5 2,695,401 - OKGIIK_12515 0.63 -0.4 2,695,401 - OKGIIK_12515 0.63 -0.3 2,695,401 - OKGIIK_12515 0.63 +0.9 2,695,401 - OKGIIK_12515 0.63 -2.1 2,695,646 - +1.1 2,695,680 + -0.9 2,695,680 + +1.0 2,695,681 - -1.8 2,695,681 - -0.5 2,695,765 + -0.3 2,695,765 + -1.6 2,695,765 + +0.6 2,695,893 - OKGIIK_12520 0.23 -1.2 2,695,893 - OKGIIK_12520 0.23 -0.9 2,695,893 - OKGIIK_12520 0.23 -0.3 2,695,953 + OKGIIK_12520 0.30 -0.1 2,695,954 - OKGIIK_12520 0.30 +0.7 2,695,954 - OKGIIK_12520 0.30 -1.3 2,695,954 - OKGIIK_12520 0.30 -0.5 2,696,077 + OKGIIK_12520 0.44 +0.7 2,696,077 + OKGIIK_12520 0.44 -0.5 2,696,078 - OKGIIK_12520 0.44 -1.1 2,696,166 + OKGIIK_12520 0.54 +0.4 2,696,166 + OKGIIK_12520 0.54 -0.5 2,696,166 + OKGIIK_12520 0.54 +0.5 2,696,167 - OKGIIK_12520 0.54 -2.2 2,696,167 - OKGIIK_12520 0.54 -1.5 2,696,167 - OKGIIK_12520 0.54 -0.6 2,696,167 - OKGIIK_12520 0.54 +1.5 2,696,167 - OKGIIK_12520 0.54 -1.1 2,696,167 - OKGIIK_12520 0.54 +0.4 2,696,173 + OKGIIK_12520 0.54 -1.8 2,696,173 + OKGIIK_12520 0.54 +0.3 2,696,173 + OKGIIK_12520 0.54 +0.4 2,696,173 + OKGIIK_12520 0.54 +0.1 2,696,173 + OKGIIK_12520 0.54 +0.5 2,696,173 + OKGIIK_12520 0.54 +2.3 2,696,174 - OKGIIK_12520 0.54 +0.7 2,696,174 - OKGIIK_12520 0.54 -0.9 2,696,174 - OKGIIK_12520 0.54 +0.2 2,696,174 - OKGIIK_12520 0.54 +2.0 2,696,174 - OKGIIK_12520 0.54 +1.4 2,696,176 + OKGIIK_12520 0.55 +0.7 2,696,177 - OKGIIK_12520 0.55 -1.5 2,696,215 + OKGIIK_12520 0.59 -0.3 2,696,215 + OKGIIK_12520 0.59 +0.5 2,696,347 + OKGIIK_12520 0.74 -0.9 2,696,347 + OKGIIK_12520 0.74 -0.1 2,696,347 + OKGIIK_12520 0.74 -0.3 2,696,347 + OKGIIK_12520 0.74 -0.3 2,696,348 - OKGIIK_12520 0.74 -1.1 2,696,348 - OKGIIK_12520 0.74 +0.0 2,696,348 - OKGIIK_12520 0.74 -2.4 2,696,348 - OKGIIK_12520 0.74 +0.4 2,696,440 + OKGIIK_12520 0.84 +1.5 2,696,440 + OKGIIK_12520 0.84 -1.9 2,696,440 + OKGIIK_12520 0.84 +0.9 2,696,440 + OKGIIK_12520 0.84 +0.9 2,696,441 - OKGIIK_12520 0.84 -0.7 2,696,441 - OKGIIK_12520 0.84 +0.6 2,696,441 - OKGIIK_12520 0.84 +1.0 2,696,441 - OKGIIK_12520 0.84 -0.2 2,696,441 - OKGIIK_12520 0.84 -0.1 2,696,441 - OKGIIK_12520 0.84 -0.9 2,696,441 - OKGIIK_12520 0.84 -0.4 2,696,584 + +1.7 2,696,584 + -0.8 2,696,584 + -1.5 2,696,584 + -1.2 2,696,584 + -0.7 2,696,584 + -1.9 2,696,584 + -1.1 2,696,585 - -0.3 2,696,585 - +1.5 2,696,585 - -0.2 2,696,585 - -0.9 2,696,610 + -0.6 2,696,617 + -0.6 2,696,635 + +0.2 2,696,635 + -1.4 2,696,636 - +0.8 2,696,636 - +0.8 2,696,636 - -0.9
Or see this region's nucleotide sequence