Experiment: R2A_PIPES pH 4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt prfA and ppk2 are separated by 106 nucleotides ppk2 and OKGIIK_10620 overlap by 4 nucleotides OKGIIK_10620 and OKGIIK_10625 are separated by 13 nucleotides OKGIIK_10625 and OKGIIK_10630 overlap by 4 nucleotides
OKGIIK_10610: prfA - peptide chain release factor 1, at 2,276,877 to 2,277,956
prfA
OKGIIK_10615: ppk2 - polyphosphate kinase 2, at 2,278,063 to 2,278,827
ppk2
OKGIIK_10620: OKGIIK_10620 - acylphosphatase, at 2,278,824 to 2,279,090
_10620
OKGIIK_10625: OKGIIK_10625 - Thioredoxin domain-containing protein, at 2,279,104 to 2,279,589
_10625
OKGIIK_10630: OKGIIK_10630 - UPF0761 membrane protein RhoFW510R10_01415, at 2,279,586 to 2,280,863
_10630
Position (kb)
2278
2279 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6
7 at 2277.982 kb on - strand at 2278.285 kb on + strand, within ppk2 at 2278.285 kb on + strand, within ppk2 at 2278.285 kb on + strand, within ppk2 at 2278.285 kb on + strand, within ppk2 at 2278.285 kb on + strand, within ppk2 at 2278.285 kb on + strand, within ppk2 at 2278.286 kb on - strand, within ppk2 at 2278.286 kb on - strand, within ppk2 at 2278.297 kb on + strand, within ppk2 at 2278.297 kb on + strand, within ppk2 at 2278.297 kb on + strand, within ppk2 at 2278.297 kb on + strand, within ppk2 at 2278.297 kb on + strand, within ppk2 at 2278.298 kb on - strand, within ppk2 at 2278.298 kb on - strand, within ppk2 at 2278.351 kb on + strand, within ppk2 at 2278.351 kb on + strand, within ppk2 at 2278.351 kb on + strand, within ppk2 at 2278.352 kb on - strand, within ppk2 at 2278.352 kb on - strand, within ppk2 at 2278.352 kb on - strand, within ppk2 at 2278.403 kb on - strand, within ppk2 at 2278.474 kb on + strand, within ppk2 at 2278.474 kb on + strand, within ppk2 at 2278.474 kb on + strand, within ppk2 at 2278.474 kb on + strand, within ppk2 at 2278.474 kb on + strand, within ppk2 at 2278.474 kb on + strand, within ppk2 at 2278.474 kb on + strand, within ppk2 at 2278.474 kb on + strand, within ppk2 at 2278.474 kb on + strand, within ppk2 at 2278.474 kb on + strand, within ppk2 at 2278.475 kb on - strand, within ppk2 at 2278.475 kb on - strand, within ppk2 at 2278.475 kb on - strand, within ppk2 at 2278.475 kb on - strand, within ppk2 at 2278.475 kb on - strand, within ppk2 at 2278.475 kb on - strand, within ppk2 at 2278.475 kb on - strand, within ppk2 at 2278.475 kb on - strand, within ppk2 at 2278.543 kb on + strand, within ppk2 at 2278.543 kb on + strand, within ppk2 at 2278.543 kb on + strand, within ppk2 at 2278.543 kb on + strand, within ppk2 at 2278.585 kb on + strand, within ppk2 at 2278.585 kb on + strand, within ppk2 at 2278.585 kb on + strand, within ppk2 at 2278.586 kb on - strand, within ppk2 at 2278.586 kb on - strand, within ppk2 at 2278.586 kb on - strand, within ppk2 at 2278.748 kb on + strand, within ppk2 at 2278.749 kb on - strand, within ppk2 at 2278.818 kb on + strand at 2278.818 kb on + strand at 2278.818 kb on + strand at 2278.818 kb on + strand at 2278.819 kb on - strand at 2278.819 kb on - strand at 2278.826 kb on - strand at 2279.041 kb on + strand, within OKGIIK_10620 at 2279.042 kb on - strand, within OKGIIK_10620 at 2279.098 kb on + strand at 2279.098 kb on + strand at 2279.099 kb on - strand at 2279.099 kb on - strand at 2279.099 kb on - strand at 2279.099 kb on - strand at 2279.157 kb on + strand, within OKGIIK_10625 at 2279.157 kb on + strand, within OKGIIK_10625 at 2279.157 kb on + strand, within OKGIIK_10625 at 2279.157 kb on + strand, within OKGIIK_10625 at 2279.157 kb on + strand, within OKGIIK_10625 at 2279.157 kb on + strand, within OKGIIK_10625 at 2279.157 kb on + strand, within OKGIIK_10625 at 2279.157 kb on + strand, within OKGIIK_10625 at 2279.158 kb on - strand, within OKGIIK_10625 at 2279.158 kb on - strand, within OKGIIK_10625 at 2279.158 kb on - strand, within OKGIIK_10625 at 2279.158 kb on - strand, within OKGIIK_10625 at 2279.158 kb on - strand, within OKGIIK_10625 at 2279.158 kb on - strand, within OKGIIK_10625 at 2279.158 kb on - strand, within OKGIIK_10625 at 2279.158 kb on - strand, within OKGIIK_10625 at 2279.158 kb on - strand, within OKGIIK_10625 at 2279.158 kb on - strand, within OKGIIK_10625 at 2279.158 kb on - strand, within OKGIIK_10625 at 2279.158 kb on - strand, within OKGIIK_10625 at 2279.192 kb on + strand, within OKGIIK_10625 at 2279.192 kb on + strand, within OKGIIK_10625 at 2279.192 kb on + strand, within OKGIIK_10625 at 2279.192 kb on + strand, within OKGIIK_10625 at 2279.192 kb on + strand, within OKGIIK_10625 at 2279.192 kb on + strand, within OKGIIK_10625 at 2279.192 kb on + strand, within OKGIIK_10625 at 2279.193 kb on - strand, within OKGIIK_10625 at 2279.193 kb on - strand, within OKGIIK_10625 at 2279.193 kb on - strand, within OKGIIK_10625 at 2279.193 kb on - strand, within OKGIIK_10625 at 2279.193 kb on - strand, within OKGIIK_10625 at 2279.193 kb on - strand, within OKGIIK_10625 at 2279.193 kb on - strand, within OKGIIK_10625 at 2279.193 kb on - strand, within OKGIIK_10625 at 2279.321 kb on + strand, within OKGIIK_10625 at 2279.321 kb on + strand, within OKGIIK_10625 at 2279.417 kb on + strand, within OKGIIK_10625 at 2279.417 kb on + strand, within OKGIIK_10625 at 2279.417 kb on + strand, within OKGIIK_10625 at 2279.417 kb on + strand, within OKGIIK_10625 at 2279.417 kb on + strand, within OKGIIK_10625 at 2279.417 kb on + strand, within OKGIIK_10625 at 2279.417 kb on + strand, within OKGIIK_10625 at 2279.418 kb on - strand, within OKGIIK_10625 at 2279.418 kb on - strand, within OKGIIK_10625 at 2279.418 kb on - strand, within OKGIIK_10625 at 2279.418 kb on - strand, within OKGIIK_10625 at 2279.418 kb on - strand, within OKGIIK_10625 at 2279.635 kb on + strand at 2279.635 kb on + strand at 2279.635 kb on + strand at 2279.635 kb on + strand at 2279.635 kb on + strand at 2279.636 kb on - strand at 2279.636 kb on - strand at 2279.636 kb on - strand at 2279.782 kb on + strand, within OKGIIK_10630 at 2279.782 kb on + strand, within OKGIIK_10630 at 2279.782 kb on + strand, within OKGIIK_10630
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES pH 4 remove 2,277,982 - +0.0 2,278,285 + ppk2 OKGIIK_10615 0.29 +1.0 2,278,285 + ppk2 OKGIIK_10615 0.29 +0.3 2,278,285 + ppk2 OKGIIK_10615 0.29 +0.0 2,278,285 + ppk2 OKGIIK_10615 0.29 +0.0 2,278,285 + ppk2 OKGIIK_10615 0.29 +0.8 2,278,285 + ppk2 OKGIIK_10615 0.29 -0.2 2,278,286 - ppk2 OKGIIK_10615 0.29 +0.8 2,278,286 - ppk2 OKGIIK_10615 0.29 -0.7 2,278,297 + ppk2 OKGIIK_10615 0.31 -0.5 2,278,297 + ppk2 OKGIIK_10615 0.31 +0.3 2,278,297 + ppk2 OKGIIK_10615 0.31 +0.9 2,278,297 + ppk2 OKGIIK_10615 0.31 +1.0 2,278,297 + ppk2 OKGIIK_10615 0.31 +2.7 2,278,298 - ppk2 OKGIIK_10615 0.31 -1.2 2,278,298 - ppk2 OKGIIK_10615 0.31 -0.5 2,278,351 + ppk2 OKGIIK_10615 0.38 -1.1 2,278,351 + ppk2 OKGIIK_10615 0.38 -0.8 2,278,351 + ppk2 OKGIIK_10615 0.38 +0.2 2,278,352 - ppk2 OKGIIK_10615 0.38 +0.3 2,278,352 - ppk2 OKGIIK_10615 0.38 -0.5 2,278,352 - ppk2 OKGIIK_10615 0.38 +1.1 2,278,403 - ppk2 OKGIIK_10615 0.44 -1.0 2,278,474 + ppk2 OKGIIK_10615 0.54 -0.2 2,278,474 + ppk2 OKGIIK_10615 0.54 +0.1 2,278,474 + ppk2 OKGIIK_10615 0.54 +0.3 2,278,474 + ppk2 OKGIIK_10615 0.54 +0.0 2,278,474 + ppk2 OKGIIK_10615 0.54 +0.5 2,278,474 + ppk2 OKGIIK_10615 0.54 +0.3 2,278,474 + ppk2 OKGIIK_10615 0.54 +0.3 2,278,474 + ppk2 OKGIIK_10615 0.54 +0.2 2,278,474 + ppk2 OKGIIK_10615 0.54 +0.3 2,278,474 + ppk2 OKGIIK_10615 0.54 +1.0 2,278,475 - ppk2 OKGIIK_10615 0.54 +2.3 2,278,475 - ppk2 OKGIIK_10615 0.54 -0.7 2,278,475 - ppk2 OKGIIK_10615 0.54 +0.3 2,278,475 - ppk2 OKGIIK_10615 0.54 +1.9 2,278,475 - ppk2 OKGIIK_10615 0.54 -0.5 2,278,475 - ppk2 OKGIIK_10615 0.54 +0.0 2,278,475 - ppk2 OKGIIK_10615 0.54 -1.3 2,278,475 - ppk2 OKGIIK_10615 0.54 -0.2 2,278,543 + ppk2 OKGIIK_10615 0.63 +0.5 2,278,543 + ppk2 OKGIIK_10615 0.63 -0.6 2,278,543 + ppk2 OKGIIK_10615 0.63 +7.3 2,278,543 + ppk2 OKGIIK_10615 0.63 -1.7 2,278,585 + ppk2 OKGIIK_10615 0.68 -1.2 2,278,585 + ppk2 OKGIIK_10615 0.68 +1.5 2,278,585 + ppk2 OKGIIK_10615 0.68 +0.3 2,278,586 - ppk2 OKGIIK_10615 0.68 -0.2 2,278,586 - ppk2 OKGIIK_10615 0.68 -0.9 2,278,586 - ppk2 OKGIIK_10615 0.68 +1.3 2,278,748 + ppk2 OKGIIK_10615 0.90 -0.8 2,278,749 - ppk2 OKGIIK_10615 0.90 -0.1 2,278,818 + -0.5 2,278,818 + -0.2 2,278,818 + -0.8 2,278,818 + -0.7 2,278,819 - +0.2 2,278,819 - -0.7 2,278,826 - -1.6 2,279,041 + OKGIIK_10620 0.81 +0.0 2,279,042 - OKGIIK_10620 0.82 +0.3 2,279,098 + -0.2 2,279,098 + +0.3 2,279,099 - +0.6 2,279,099 - -1.1 2,279,099 - +0.1 2,279,099 - +6.0 2,279,157 + OKGIIK_10625 0.11 -0.5 2,279,157 + OKGIIK_10625 0.11 -1.2 2,279,157 + OKGIIK_10625 0.11 -0.8 2,279,157 + OKGIIK_10625 0.11 -0.8 2,279,157 + OKGIIK_10625 0.11 -1.0 2,279,157 + OKGIIK_10625 0.11 -1.0 2,279,157 + OKGIIK_10625 0.11 -0.2 2,279,157 + OKGIIK_10625 0.11 +1.0 2,279,158 - OKGIIK_10625 0.11 +0.3 2,279,158 - OKGIIK_10625 0.11 -1.5 2,279,158 - OKGIIK_10625 0.11 +0.3 2,279,158 - OKGIIK_10625 0.11 -0.8 2,279,158 - OKGIIK_10625 0.11 -0.7 2,279,158 - OKGIIK_10625 0.11 -0.2 2,279,158 - OKGIIK_10625 0.11 +2.0 2,279,158 - OKGIIK_10625 0.11 -1.8 2,279,158 - OKGIIK_10625 0.11 -1.2 2,279,158 - OKGIIK_10625 0.11 -2.5 2,279,158 - OKGIIK_10625 0.11 -0.2 2,279,158 - OKGIIK_10625 0.11 -2.0 2,279,192 + OKGIIK_10625 0.18 -0.5 2,279,192 + OKGIIK_10625 0.18 -0.8 2,279,192 + OKGIIK_10625 0.18 -1.1 2,279,192 + OKGIIK_10625 0.18 +0.1 2,279,192 + OKGIIK_10625 0.18 -1.6 2,279,192 + OKGIIK_10625 0.18 -0.2 2,279,192 + OKGIIK_10625 0.18 +2.3 2,279,193 - OKGIIK_10625 0.18 -1.1 2,279,193 - OKGIIK_10625 0.18 -1.7 2,279,193 - OKGIIK_10625 0.18 +0.3 2,279,193 - OKGIIK_10625 0.18 -1.7 2,279,193 - OKGIIK_10625 0.18 +0.3 2,279,193 - OKGIIK_10625 0.18 +0.3 2,279,193 - OKGIIK_10625 0.18 -0.2 2,279,193 - OKGIIK_10625 0.18 -0.5 2,279,321 + OKGIIK_10625 0.45 +0.3 2,279,321 + OKGIIK_10625 0.45 -0.2 2,279,417 + OKGIIK_10625 0.64 -1.0 2,279,417 + OKGIIK_10625 0.64 +0.0 2,279,417 + OKGIIK_10625 0.64 -2.1 2,279,417 + OKGIIK_10625 0.64 +0.3 2,279,417 + OKGIIK_10625 0.64 -1.2 2,279,417 + OKGIIK_10625 0.64 -1.2 2,279,417 + OKGIIK_10625 0.64 +0.8 2,279,418 - OKGIIK_10625 0.65 -0.4 2,279,418 - OKGIIK_10625 0.65 -1.8 2,279,418 - OKGIIK_10625 0.65 -1.7 2,279,418 - OKGIIK_10625 0.65 +0.3 2,279,418 - OKGIIK_10625 0.65 +1.1 2,279,635 + +0.3 2,279,635 + -1.7 2,279,635 + +0.3 2,279,635 + -0.2 2,279,635 + -0.2 2,279,636 - -0.4 2,279,636 - +0.0 2,279,636 - -0.4 2,279,782 + OKGIIK_10630 0.15 +0.0 2,279,782 + OKGIIK_10630 0.15 +0.0 2,279,782 + OKGIIK_10630 0.15 -0.8
Or see this region's nucleotide sequence