Experiment: R2A_PIPES pH 4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt prs and Gln_trna are separated by 33 nucleotides Gln_trna and ispE are separated by 63 nucleotides ispE and lolB are separated by 50 nucleotides lolB and OKGIIK_10600 overlap by 4 nucleotides
OKGIIK_10580: prs - ribose-phosphate diphosphokinase, at 2,270,475 to 2,271,434
prs
OKGIIK_10585: Gln_trna - tRNA-Gln, at 2,271,468 to 2,271,544
_trna
OKGIIK_10590: ispE - 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase, at 2,271,608 to 2,273,008
ispE
OKGIIK_10595: lolB - lipoprotein insertase outer membrane protein LolB, at 2,273,059 to 2,273,670
lolB
OKGIIK_10600: OKGIIK_10600 - Beta-barrel assembly-enhancing protease, at 2,273,667 to 2,275,424
_10600
Position (kb)
2271
2272
2273
2274 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2271.442 kb on - strand at 2271.442 kb on - strand at 2271.442 kb on - strand at 2271.442 kb on - strand at 2271.442 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.550 kb on - strand at 2271.574 kb on - strand at 2271.574 kb on - strand at 2271.574 kb on - strand at 2271.574 kb on - strand at 2271.574 kb on - strand at 2271.574 kb on - strand at 2271.574 kb on - strand at 2271.574 kb on - strand at 2271.574 kb on - strand at 2271.574 kb on - strand at 2271.574 kb on - strand at 2271.574 kb on - strand at 2271.574 kb on - strand at 2271.574 kb on - strand at 2271.574 kb on - strand at 2271.574 kb on - strand at 2271.574 kb on - strand at 2271.574 kb on - strand at 2271.574 kb on - strand at 2271.574 kb on - strand at 2271.574 kb on - strand at 2271.574 kb on - strand at 2271.621 kb on - strand at 2271.621 kb on - strand at 2271.621 kb on - strand at 2271.621 kb on - strand at 2272.469 kb on + strand, within ispE at 2272.470 kb on - strand, within ispE at 2272.470 kb on - strand, within ispE at 2272.618 kb on - strand, within ispE at 2272.618 kb on - strand, within ispE at 2272.618 kb on - strand, within ispE at 2272.638 kb on - strand, within ispE at 2272.638 kb on - strand, within ispE at 2272.638 kb on - strand, within ispE at 2272.638 kb on - strand, within ispE at 2272.638 kb on - strand, within ispE at 2272.638 kb on - strand, within ispE at 2272.638 kb on - strand, within ispE at 2272.638 kb on - strand, within ispE at 2272.638 kb on - strand, within ispE at 2272.638 kb on - strand, within ispE at 2272.638 kb on - strand, within ispE at 2272.738 kb on - strand, within ispE at 2272.738 kb on - strand, within ispE at 2272.738 kb on - strand, within ispE at 2272.903 kb on - strand at 2272.903 kb on - strand at 2272.903 kb on - strand at 2272.903 kb on - strand at 2272.903 kb on - strand at 2272.903 kb on - strand at 2272.903 kb on - strand at 2272.903 kb on - strand at 2273.052 kb on + strand at 2273.052 kb on + strand at 2273.052 kb on + strand at 2273.052 kb on + strand at 2273.052 kb on + strand at 2273.053 kb on - strand at 2273.053 kb on - strand at 2273.872 kb on + strand, within OKGIIK_10600 at 2273.873 kb on - strand, within OKGIIK_10600 at 2273.987 kb on - strand, within OKGIIK_10600 at 2273.987 kb on - strand, within OKGIIK_10600 at 2273.987 kb on - strand, within OKGIIK_10600 at 2273.987 kb on - strand, within OKGIIK_10600 at 2273.987 kb on - strand, within OKGIIK_10600 at 2273.987 kb on - strand, within OKGIIK_10600 at 2273.987 kb on - strand, within OKGIIK_10600 at 2273.987 kb on - strand, within OKGIIK_10600 at 2273.987 kb on - strand, within OKGIIK_10600 at 2273.987 kb on - strand, within OKGIIK_10600 at 2273.987 kb on - strand, within OKGIIK_10600
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES pH 4 remove 2,271,442 - -1.7 2,271,442 - +0.2 2,271,442 - -1.0 2,271,442 - -1.8 2,271,442 - +1.3 2,271,550 - -3.6 2,271,550 - -1.6 2,271,550 - -0.8 2,271,550 - -0.7 2,271,550 - -1.7 2,271,550 - -1.7 2,271,550 - -0.4 2,271,550 - -0.7 2,271,550 - +0.0 2,271,550 - -1.6 2,271,550 - -3.4 2,271,550 - +0.0 2,271,550 - -1.4 2,271,550 - -2.2 2,271,550 - -1.6 2,271,550 - -1.4 2,271,550 - -2.9 2,271,550 - +0.0 2,271,550 - +0.3 2,271,550 - -0.4 2,271,550 - -0.4 2,271,550 - -1.2 2,271,550 - -1.6 2,271,550 - -2.8 2,271,550 - -1.0 2,271,550 - -0.2 2,271,550 - -1.6 2,271,550 - -0.4 2,271,550 - -1.2 2,271,550 - -2.1 2,271,550 - -1.5 2,271,550 - -1.2 2,271,550 - -0.7 2,271,550 - -0.4 2,271,550 - -2.2 2,271,550 - -1.6 2,271,550 - -1.0 2,271,550 - -1.0 2,271,550 - -2.0 2,271,550 - +0.6 2,271,550 - -1.3 2,271,550 - -1.0 2,271,550 - -1.0 2,271,550 - -0.6 2,271,550 - -3.2 2,271,550 - -1.2 2,271,550 - -2.7 2,271,550 - -1.0 2,271,550 - +1.6 2,271,550 - -0.8 2,271,550 - -0.4 2,271,574 - +0.4 2,271,574 - +1.4 2,271,574 - -0.2 2,271,574 - +0.0 2,271,574 - -0.6 2,271,574 - -0.4 2,271,574 - -0.7 2,271,574 - -0.5 2,271,574 - +0.3 2,271,574 - -1.0 2,271,574 - -0.6 2,271,574 - -1.0 2,271,574 - +0.9 2,271,574 - -1.0 2,271,574 - -1.5 2,271,574 - -1.6 2,271,574 - -1.3 2,271,574 - -1.7 2,271,574 - -0.5 2,271,574 - -0.6 2,271,574 - -2.2 2,271,574 - -1.7 2,271,621 - -0.4 2,271,621 - -1.2 2,271,621 - -0.4 2,271,621 - -1.0 2,272,469 + ispE OKGIIK_10590 0.61 -1.0 2,272,470 - ispE OKGIIK_10590 0.62 -2.7 2,272,470 - ispE OKGIIK_10590 0.62 -1.0 2,272,618 - ispE OKGIIK_10590 0.72 -2.4 2,272,618 - ispE OKGIIK_10590 0.72 -0.8 2,272,618 - ispE OKGIIK_10590 0.72 -0.1 2,272,638 - ispE OKGIIK_10590 0.74 -2.4 2,272,638 - ispE OKGIIK_10590 0.74 -2.6 2,272,638 - ispE OKGIIK_10590 0.74 -2.7 2,272,638 - ispE OKGIIK_10590 0.74 -0.7 2,272,638 - ispE OKGIIK_10590 0.74 -1.4 2,272,638 - ispE OKGIIK_10590 0.74 -1.0 2,272,638 - ispE OKGIIK_10590 0.74 +0.3 2,272,638 - ispE OKGIIK_10590 0.74 -0.4 2,272,638 - ispE OKGIIK_10590 0.74 -0.4 2,272,638 - ispE OKGIIK_10590 0.74 -0.4 2,272,638 - ispE OKGIIK_10590 0.74 -2.2 2,272,738 - ispE OKGIIK_10590 0.81 -1.3 2,272,738 - ispE OKGIIK_10590 0.81 -0.6 2,272,738 - ispE OKGIIK_10590 0.81 -1.7 2,272,903 - +0.0 2,272,903 - -0.7 2,272,903 - -1.0 2,272,903 - -1.4 2,272,903 - -0.7 2,272,903 - -0.7 2,272,903 - -2.0 2,272,903 - -1.0 2,273,052 + -0.4 2,273,052 + +0.6 2,273,052 + -2.5 2,273,052 + -0.8 2,273,052 + +1.3 2,273,053 - -1.6 2,273,053 - -0.8 2,273,872 + OKGIIK_10600 0.12 -1.8 2,273,873 - OKGIIK_10600 0.12 -1.0 2,273,987 - OKGIIK_10600 0.18 -1.0 2,273,987 - OKGIIK_10600 0.18 -1.7 2,273,987 - OKGIIK_10600 0.18 -0.7 2,273,987 - OKGIIK_10600 0.18 -0.4 2,273,987 - OKGIIK_10600 0.18 +0.2 2,273,987 - OKGIIK_10600 0.18 -0.4 2,273,987 - OKGIIK_10600 0.18 -1.4 2,273,987 - OKGIIK_10600 0.18 -1.6 2,273,987 - OKGIIK_10600 0.18 -0.4 2,273,987 - OKGIIK_10600 0.18 -0.7 2,273,987 - OKGIIK_10600 0.18 -1.2
Or see this region's nucleotide sequence