Experiment: R2A_PIPES pH 4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nusG and secE are separated by 7 nucleotides secE and Trp_trna are separated by 82 nucleotides Trp_trna and tuf are separated by 192 nucleotides tuf and Thr_trna are separated by 77 nucleotides Thr_trna and Gly_trna are separated by 136 nucleotides Gly_trna and Tyr_trna are separated by 18 nucleotides Tyr_trna and yciA are separated by 93 nucleotides
OKGIIK_10525: nusG - transcription termination/antitermination protein NusG, at 2,264,152 to 2,264,712
nusG
OKGIIK_10530: secE - preprotein translocase subunit SecE, at 2,264,720 to 2,265,115
secE
OKGIIK_10535: Trp_trna - tRNA-Trp, at 2,265,198 to 2,265,273
_trna
OKGIIK_10540: tuf - elongation factor Tu, at 2,265,466 to 2,266,656
tuf
OKGIIK_10545: Thr_trna - tRNA-Thr, at 2,266,734 to 2,266,809
_trna
OKGIIK_10550: Gly_trna - tRNA-Gly, at 2,266,946 to 2,267,019
_trna
OKGIIK_10555: Tyr_trna - tRNA-Tyr, at 2,267,038 to 2,267,123
_trna
OKGIIK_10560: yciA - acyl-CoA thioesterase, at 2,267,217 to 2,267,705
yciA
Position (kb)
2265
2266
2267 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2265.335 kb on - strand at 2265.335 kb on - strand at 2265.335 kb on - strand at 2265.335 kb on - strand at 2265.335 kb on - strand at 2265.399 kb on - strand at 2265.399 kb on - strand at 2265.399 kb on - strand at 2265.399 kb on - strand at 2265.399 kb on - strand at 2265.468 kb on - strand at 2265.468 kb on - strand at 2265.468 kb on - strand at 2265.468 kb on - strand at 2265.468 kb on - strand at 2265.468 kb on - strand at 2265.468 kb on - strand at 2265.468 kb on - strand at 2265.468 kb on - strand at 2265.468 kb on - strand at 2265.468 kb on - strand at 2265.468 kb on - strand at 2265.468 kb on - strand at 2265.468 kb on - strand at 2265.468 kb on - strand at 2265.468 kb on - strand at 2265.468 kb on - strand at 2265.468 kb on - strand at 2265.468 kb on - strand at 2265.468 kb on - strand at 2265.468 kb on - strand at 2265.468 kb on - strand at 2265.468 kb on - strand at 2265.468 kb on - strand at 2265.468 kb on - strand at 2265.468 kb on - strand at 2265.468 kb on - strand at 2265.468 kb on - strand at 2265.468 kb on - strand at 2265.468 kb on - strand at 2265.657 kb on - strand, within tuf at 2265.657 kb on - strand, within tuf at 2265.657 kb on - strand, within tuf at 2265.657 kb on - strand, within tuf at 2265.691 kb on - strand, within tuf at 2265.691 kb on - strand, within tuf at 2265.691 kb on - strand, within tuf at 2265.691 kb on - strand, within tuf at 2265.691 kb on - strand, within tuf at 2266.814 kb on - strand at 2266.814 kb on - strand at 2266.814 kb on - strand at 2266.814 kb on - strand at 2266.814 kb on - strand at 2266.814 kb on - strand at 2266.814 kb on - strand at 2266.814 kb on - strand at 2266.814 kb on - strand at 2266.814 kb on - strand at 2266.814 kb on - strand at 2266.814 kb on - strand at 2266.814 kb on - strand at 2266.939 kb on - strand at 2266.939 kb on - strand at 2266.939 kb on - strand at 2266.939 kb on - strand at 2266.939 kb on - strand at 2266.939 kb on - strand at 2266.939 kb on - strand at 2266.939 kb on - strand at 2267.293 kb on - strand, within yciA at 2267.293 kb on - strand, within yciA at 2267.293 kb on - strand, within yciA at 2267.293 kb on - strand, within yciA at 2267.293 kb on - strand, within yciA at 2267.293 kb on - strand, within yciA at 2267.293 kb on - strand, within yciA at 2267.293 kb on - strand, within yciA at 2267.293 kb on - strand, within yciA at 2267.293 kb on - strand, within yciA at 2267.560 kb on + strand, within yciA at 2267.560 kb on + strand, within yciA at 2267.560 kb on + strand, within yciA at 2267.560 kb on + strand, within yciA at 2267.560 kb on + strand, within yciA at 2267.560 kb on + strand, within yciA at 2267.560 kb on + strand, within yciA at 2267.560 kb on + strand, within yciA at 2267.561 kb on - strand, within yciA at 2267.561 kb on - strand, within yciA at 2267.561 kb on - strand, within yciA at 2267.561 kb on - strand, within yciA at 2267.561 kb on - strand, within yciA at 2267.561 kb on - strand, within yciA at 2267.561 kb on - strand, within yciA at 2267.561 kb on - strand, within yciA
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES pH 4 remove 2,265,335 - -0.4 2,265,335 - -2.1 2,265,335 - -0.4 2,265,335 - -0.6 2,265,335 - -1.2 2,265,399 - -2.4 2,265,399 - +0.8 2,265,399 - -1.4 2,265,399 - +0.3 2,265,399 - +0.4 2,265,468 - -0.6 2,265,468 - -1.8 2,265,468 - +0.6 2,265,468 - +1.0 2,265,468 - -1.0 2,265,468 - +0.4 2,265,468 - -1.2 2,265,468 - -0.1 2,265,468 - +0.0 2,265,468 - -1.4 2,265,468 - -0.4 2,265,468 - -1.7 2,265,468 - -1.4 2,265,468 - -1.2 2,265,468 - +0.9 2,265,468 - -0.7 2,265,468 - -0.5 2,265,468 - -1.8 2,265,468 - -1.0 2,265,468 - -1.6 2,265,468 - -1.4 2,265,468 - -0.7 2,265,468 - -0.4 2,265,468 - -0.4 2,265,468 - +0.6 2,265,468 - -1.5 2,265,468 - -0.7 2,265,468 - -2.1 2,265,468 - -1.0 2,265,468 - -0.4 2,265,657 - tuf OKGIIK_10540 0.16 -1.7 2,265,657 - tuf OKGIIK_10540 0.16 -1.4 2,265,657 - tuf OKGIIK_10540 0.16 -0.7 2,265,657 - tuf OKGIIK_10540 0.16 -0.4 2,265,691 - tuf OKGIIK_10540 0.19 +0.4 2,265,691 - tuf OKGIIK_10540 0.19 -1.8 2,265,691 - tuf OKGIIK_10540 0.19 -1.6 2,265,691 - tuf OKGIIK_10540 0.19 -1.7 2,265,691 - tuf OKGIIK_10540 0.19 -0.7 2,266,814 - +0.8 2,266,814 - -0.4 2,266,814 - -1.5 2,266,814 - +0.4 2,266,814 - -2.4 2,266,814 - -2.1 2,266,814 - -0.4 2,266,814 - -0.5 2,266,814 - -1.0 2,266,814 - -1.7 2,266,814 - -1.7 2,266,814 - -1.6 2,266,814 - -1.7 2,266,939 - -0.3 2,266,939 - -1.0 2,266,939 - -0.4 2,266,939 - -0.7 2,266,939 - -0.7 2,266,939 - -1.5 2,266,939 - -0.4 2,266,939 - +0.0 2,267,293 - yciA OKGIIK_10560 0.16 -0.7 2,267,293 - yciA OKGIIK_10560 0.16 -0.4 2,267,293 - yciA OKGIIK_10560 0.16 -1.7 2,267,293 - yciA OKGIIK_10560 0.16 +0.8 2,267,293 - yciA OKGIIK_10560 0.16 -1.0 2,267,293 - yciA OKGIIK_10560 0.16 -0.2 2,267,293 - yciA OKGIIK_10560 0.16 +0.3 2,267,293 - yciA OKGIIK_10560 0.16 -0.5 2,267,293 - yciA OKGIIK_10560 0.16 +1.0 2,267,293 - yciA OKGIIK_10560 0.16 -3.0 2,267,560 + yciA OKGIIK_10560 0.70 -0.4 2,267,560 + yciA OKGIIK_10560 0.70 -0.1 2,267,560 + yciA OKGIIK_10560 0.70 +0.3 2,267,560 + yciA OKGIIK_10560 0.70 -0.2 2,267,560 + yciA OKGIIK_10560 0.70 -1.8 2,267,560 + yciA OKGIIK_10560 0.70 -1.0 2,267,560 + yciA OKGIIK_10560 0.70 -1.0 2,267,560 + yciA OKGIIK_10560 0.70 -1.0 2,267,561 - yciA OKGIIK_10560 0.70 +1.3 2,267,561 - yciA OKGIIK_10560 0.70 -1.2 2,267,561 - yciA OKGIIK_10560 0.70 -1.0 2,267,561 - yciA OKGIIK_10560 0.70 -1.8 2,267,561 - yciA OKGIIK_10560 0.70 +0.0 2,267,561 - yciA OKGIIK_10560 0.70 -0.4 2,267,561 - yciA OKGIIK_10560 0.70 +0.9 2,267,561 - yciA OKGIIK_10560 0.70 -1.5
Or see this region's nucleotide sequence