Experiment: R2A_PIPES pH 4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt argC and argB are separated by 61 nucleotides argB and OKGIIK_09445 are separated by 20 nucleotides
OKGIIK_09435: argC - N-acetyl-gamma-glutamyl-phosphate reductase, at 2,047,026 to 2,047,985
argC
OKGIIK_09440: argB - acetylglutamate kinase, at 2,048,047 to 2,049,381
argB
OKGIIK_09445: OKGIIK_09445 - acetylornithine deacetylase, at 2,049,402 to 2,050,535
_09445
Position (kb)
2048
2049
2050 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2047.051 kb on + strand at 2047.051 kb on + strand at 2047.051 kb on + strand at 2047.051 kb on + strand at 2047.051 kb on + strand at 2047.052 kb on - strand at 2047.052 kb on - strand at 2047.230 kb on + strand, within argC at 2047.231 kb on - strand, within argC at 2047.255 kb on + strand, within argC at 2047.255 kb on + strand, within argC at 2047.255 kb on + strand, within argC at 2047.255 kb on + strand, within argC at 2047.255 kb on + strand, within argC at 2047.256 kb on - strand, within argC at 2047.309 kb on + strand, within argC at 2047.309 kb on + strand, within argC at 2047.309 kb on + strand, within argC at 2047.309 kb on + strand, within argC at 2047.309 kb on + strand, within argC at 2047.309 kb on + strand, within argC at 2047.309 kb on + strand, within argC at 2047.309 kb on + strand, within argC at 2047.309 kb on + strand, within argC at 2047.310 kb on - strand, within argC at 2047.310 kb on - strand, within argC at 2047.310 kb on - strand, within argC at 2047.576 kb on + strand, within argC at 2047.577 kb on - strand, within argC at 2047.577 kb on - strand, within argC at 2047.577 kb on - strand, within argC at 2047.577 kb on - strand, within argC at 2047.642 kb on + strand, within argC at 2047.643 kb on - strand, within argC at 2047.643 kb on - strand, within argC at 2047.645 kb on + strand, within argC at 2047.645 kb on + strand, within argC at 2047.645 kb on + strand, within argC at 2047.646 kb on - strand, within argC at 2047.646 kb on - strand, within argC at 2047.646 kb on - strand, within argC at 2047.646 kb on - strand, within argC at 2047.646 kb on - strand, within argC at 2047.646 kb on - strand, within argC at 2047.646 kb on - strand, within argC at 2047.666 kb on + strand, within argC at 2048.025 kb on + strand at 2048.025 kb on + strand at 2048.025 kb on + strand at 2048.025 kb on + strand at 2048.026 kb on - strand at 2048.026 kb on - strand at 2048.177 kb on + strand at 2048.177 kb on + strand at 2048.177 kb on + strand at 2048.177 kb on + strand at 2048.177 kb on + strand at 2048.177 kb on + strand at 2048.178 kb on - strand at 2048.178 kb on - strand at 2048.178 kb on - strand at 2048.178 kb on - strand at 2048.180 kb on + strand at 2048.180 kb on + strand at 2048.180 kb on + strand at 2048.181 kb on - strand, within argB at 2048.181 kb on - strand, within argB at 2048.181 kb on - strand, within argB at 2048.181 kb on - strand, within argB at 2048.312 kb on + strand, within argB at 2048.312 kb on + strand, within argB at 2048.312 kb on + strand, within argB at 2048.313 kb on - strand, within argB at 2048.369 kb on + strand, within argB at 2048.369 kb on + strand, within argB at 2048.370 kb on - strand, within argB at 2048.370 kb on - strand, within argB at 2048.370 kb on - strand, within argB at 2048.370 kb on - strand, within argB at 2048.384 kb on + strand, within argB at 2048.384 kb on + strand, within argB at 2048.384 kb on + strand, within argB at 2048.384 kb on + strand, within argB at 2048.384 kb on + strand, within argB at 2048.384 kb on + strand, within argB at 2048.384 kb on + strand, within argB at 2048.385 kb on - strand, within argB at 2048.385 kb on - strand, within argB at 2048.414 kb on + strand, within argB at 2048.414 kb on + strand, within argB at 2048.415 kb on - strand, within argB at 2048.415 kb on - strand, within argB at 2048.771 kb on + strand, within argB at 2048.771 kb on + strand, within argB at 2048.771 kb on + strand, within argB at 2048.771 kb on + strand, within argB at 2048.772 kb on - strand, within argB at 2048.772 kb on - strand, within argB at 2048.772 kb on - strand, within argB at 2048.957 kb on + strand, within argB at 2048.958 kb on - strand, within argB at 2048.958 kb on - strand, within argB at 2048.958 kb on - strand, within argB at 2048.958 kb on - strand, within argB at 2048.958 kb on - strand, within argB at 2048.958 kb on - strand, within argB at 2048.958 kb on - strand, within argB at 2048.958 kb on - strand, within argB at 2048.958 kb on - strand, within argB at 2049.302 kb on + strand at 2049.302 kb on + strand at 2049.302 kb on + strand at 2049.303 kb on - strand at 2049.303 kb on - strand at 2049.303 kb on - strand at 2049.303 kb on - strand at 2049.303 kb on - strand at 2049.303 kb on - strand at 2049.303 kb on - strand at 2049.303 kb on - strand at 2049.385 kb on + strand at 2049.385 kb on + strand at 2049.385 kb on + strand at 2049.385 kb on + strand at 2049.386 kb on - strand at 2049.386 kb on - strand at 2049.386 kb on - strand at 2049.478 kb on + strand at 2049.479 kb on - strand at 2049.479 kb on - strand at 2049.548 kb on - strand, within OKGIIK_09445 at 2050.096 kb on + strand, within OKGIIK_09445 at 2050.096 kb on + strand, within OKGIIK_09445 at 2050.096 kb on + strand, within OKGIIK_09445 at 2050.097 kb on - strand, within OKGIIK_09445 at 2050.097 kb on - strand, within OKGIIK_09445 at 2050.097 kb on - strand, within OKGIIK_09445 at 2050.097 kb on - strand, within OKGIIK_09445 at 2050.097 kb on - strand, within OKGIIK_09445 at 2050.097 kb on - strand, within OKGIIK_09445 at 2050.097 kb on - strand, within OKGIIK_09445
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES pH 4 remove 2,047,051 + +1.5 2,047,051 + +0.5 2,047,051 + +0.7 2,047,051 + -0.7 2,047,051 + -0.4 2,047,052 - +0.4 2,047,052 - +0.4 2,047,230 + argC OKGIIK_09435 0.21 +0.5 2,047,231 - argC OKGIIK_09435 0.21 +0.1 2,047,255 + argC OKGIIK_09435 0.24 -1.4 2,047,255 + argC OKGIIK_09435 0.24 -0.2 2,047,255 + argC OKGIIK_09435 0.24 +1.6 2,047,255 + argC OKGIIK_09435 0.24 +1.3 2,047,255 + argC OKGIIK_09435 0.24 +0.8 2,047,256 - argC OKGIIK_09435 0.24 -0.4 2,047,309 + argC OKGIIK_09435 0.29 +0.4 2,047,309 + argC OKGIIK_09435 0.29 -0.3 2,047,309 + argC OKGIIK_09435 0.29 +0.6 2,047,309 + argC OKGIIK_09435 0.29 -0.4 2,047,309 + argC OKGIIK_09435 0.29 +0.5 2,047,309 + argC OKGIIK_09435 0.29 -0.2 2,047,309 + argC OKGIIK_09435 0.29 -1.3 2,047,309 + argC OKGIIK_09435 0.29 +0.2 2,047,309 + argC OKGIIK_09435 0.29 -0.4 2,047,310 - argC OKGIIK_09435 0.30 -1.4 2,047,310 - argC OKGIIK_09435 0.30 -0.2 2,047,310 - argC OKGIIK_09435 0.30 +0.4 2,047,576 + argC OKGIIK_09435 0.57 +1.8 2,047,577 - argC OKGIIK_09435 0.57 +2.2 2,047,577 - argC OKGIIK_09435 0.57 -1.1 2,047,577 - argC OKGIIK_09435 0.57 -1.1 2,047,577 - argC OKGIIK_09435 0.57 +0.1 2,047,642 + argC OKGIIK_09435 0.64 -1.7 2,047,643 - argC OKGIIK_09435 0.64 +0.4 2,047,643 - argC OKGIIK_09435 0.64 +0.8 2,047,645 + argC OKGIIK_09435 0.64 -0.2 2,047,645 + argC OKGIIK_09435 0.64 +0.4 2,047,645 + argC OKGIIK_09435 0.64 -1.8 2,047,646 - argC OKGIIK_09435 0.65 +0.5 2,047,646 - argC OKGIIK_09435 0.65 +0.4 2,047,646 - argC OKGIIK_09435 0.65 +0.1 2,047,646 - argC OKGIIK_09435 0.65 +0.8 2,047,646 - argC OKGIIK_09435 0.65 -0.2 2,047,646 - argC OKGIIK_09435 0.65 +0.1 2,047,646 - argC OKGIIK_09435 0.65 +0.7 2,047,666 + argC OKGIIK_09435 0.67 -1.1 2,048,025 + -1.5 2,048,025 + -1.2 2,048,025 + -0.6 2,048,025 + -0.6 2,048,026 - -1.4 2,048,026 - +1.0 2,048,177 + +1.0 2,048,177 + +1.1 2,048,177 + -0.2 2,048,177 + -0.6 2,048,177 + -0.6 2,048,177 + -1.5 2,048,178 - +0.1 2,048,178 - -1.9 2,048,178 - -0.7 2,048,178 - +1.0 2,048,180 + -1.1 2,048,180 + +0.7 2,048,180 + +1.3 2,048,181 - argB OKGIIK_09440 0.10 -0.2 2,048,181 - argB OKGIIK_09440 0.10 -1.3 2,048,181 - argB OKGIIK_09440 0.10 +1.3 2,048,181 - argB OKGIIK_09440 0.10 +1.0 2,048,312 + argB OKGIIK_09440 0.20 +2.1 2,048,312 + argB OKGIIK_09440 0.20 -0.9 2,048,312 + argB OKGIIK_09440 0.20 +1.1 2,048,313 - argB OKGIIK_09440 0.20 +1.4 2,048,369 + argB OKGIIK_09440 0.24 -0.7 2,048,369 + argB OKGIIK_09440 0.24 +0.1 2,048,370 - argB OKGIIK_09440 0.24 -2.5 2,048,370 - argB OKGIIK_09440 0.24 -0.2 2,048,370 - argB OKGIIK_09440 0.24 -0.3 2,048,370 - argB OKGIIK_09440 0.24 +0.2 2,048,384 + argB OKGIIK_09440 0.25 -2.2 2,048,384 + argB OKGIIK_09440 0.25 +0.1 2,048,384 + argB OKGIIK_09440 0.25 -0.4 2,048,384 + argB OKGIIK_09440 0.25 -2.3 2,048,384 + argB OKGIIK_09440 0.25 +1.4 2,048,384 + argB OKGIIK_09440 0.25 -0.4 2,048,384 + argB OKGIIK_09440 0.25 -0.6 2,048,385 - argB OKGIIK_09440 0.25 +0.8 2,048,385 - argB OKGIIK_09440 0.25 +0.4 2,048,414 + argB OKGIIK_09440 0.27 +1.4 2,048,414 + argB OKGIIK_09440 0.27 +0.4 2,048,415 - argB OKGIIK_09440 0.28 +0.4 2,048,415 - argB OKGIIK_09440 0.28 +1.3 2,048,771 + argB OKGIIK_09440 0.54 -0.2 2,048,771 + argB OKGIIK_09440 0.54 +1.2 2,048,771 + argB OKGIIK_09440 0.54 -0.2 2,048,771 + argB OKGIIK_09440 0.54 +0.1 2,048,772 - argB OKGIIK_09440 0.54 +1.2 2,048,772 - argB OKGIIK_09440 0.54 +1.2 2,048,772 - argB OKGIIK_09440 0.54 +2.6 2,048,957 + argB OKGIIK_09440 0.68 +1.4 2,048,958 - argB OKGIIK_09440 0.68 -0.1 2,048,958 - argB OKGIIK_09440 0.68 +0.4 2,048,958 - argB OKGIIK_09440 0.68 +0.4 2,048,958 - argB OKGIIK_09440 0.68 -1.6 2,048,958 - argB OKGIIK_09440 0.68 -0.5 2,048,958 - argB OKGIIK_09440 0.68 +0.7 2,048,958 - argB OKGIIK_09440 0.68 +0.4 2,048,958 - argB OKGIIK_09440 0.68 -1.2 2,048,958 - argB OKGIIK_09440 0.68 -0.1 2,049,302 + -0.2 2,049,302 + +0.1 2,049,302 + -2.0 2,049,303 - -0.8 2,049,303 - +1.4 2,049,303 - +0.1 2,049,303 - -0.8 2,049,303 - +0.4 2,049,303 - -3.1 2,049,303 - +1.0 2,049,303 - +0.1 2,049,385 + +1.4 2,049,385 + -0.6 2,049,385 + +1.0 2,049,385 + -0.4 2,049,386 - -0.4 2,049,386 - +1.4 2,049,386 - -1.2 2,049,478 + -0.1 2,049,479 - +1.1 2,049,479 - +2.0 2,049,548 - OKGIIK_09445 0.13 -0.2 2,050,096 + OKGIIK_09445 0.61 -1.1 2,050,096 + OKGIIK_09445 0.61 -0.6 2,050,096 + OKGIIK_09445 0.61 +0.4 2,050,097 - OKGIIK_09445 0.61 -1.1 2,050,097 - OKGIIK_09445 0.61 -1.7 2,050,097 - OKGIIK_09445 0.61 -0.4 2,050,097 - OKGIIK_09445 0.61 -0.4 2,050,097 - OKGIIK_09445 0.61 +0.1 2,050,097 - OKGIIK_09445 0.61 -0.4 2,050,097 - OKGIIK_09445 0.61 -1.5
Or see this region's nucleotide sequence