Experiment: R2A_PIPES pH 4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OKGIIK_04935 and yerC are separated by 289 nucleotides yerC and hisG overlap by 4 nucleotides hisG and hisD are separated by 28 nucleotides
OKGIIK_04935: OKGIIK_04935 - hypothetical protein, at 1,071,696 to 1,072,709
_04935
OKGIIK_04940: yerC - TrpR , YerC/YecD, at 1,072,999 to 1,073,334
yerC
OKGIIK_04945: hisG - ATP phosphoribosyltransferase, at 1,073,331 to 1,074,224
hisG
OKGIIK_04950: hisD - histidinol dehydrogenase, at 1,074,253 to 1,075,551
hisD
Position (kb)
1072
1073
1074 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1072.036 kb on + strand, within OKGIIK_04935 at 1072.037 kb on - strand, within OKGIIK_04935 at 1072.037 kb on - strand, within OKGIIK_04935 at 1072.037 kb on - strand, within OKGIIK_04935 at 1072.037 kb on - strand, within OKGIIK_04935 at 1072.037 kb on - strand, within OKGIIK_04935 at 1072.037 kb on - strand, within OKGIIK_04935 at 1072.058 kb on - strand, within OKGIIK_04935 at 1072.058 kb on - strand, within OKGIIK_04935 at 1072.070 kb on - strand, within OKGIIK_04935 at 1072.070 kb on - strand, within OKGIIK_04935 at 1072.327 kb on + strand, within OKGIIK_04935 at 1072.327 kb on + strand, within OKGIIK_04935 at 1072.328 kb on - strand, within OKGIIK_04935 at 1072.328 kb on - strand, within OKGIIK_04935 at 1072.328 kb on - strand, within OKGIIK_04935 at 1072.354 kb on + strand, within OKGIIK_04935 at 1072.369 kb on + strand, within OKGIIK_04935 at 1072.370 kb on - strand, within OKGIIK_04935 at 1072.370 kb on - strand, within OKGIIK_04935 at 1072.370 kb on - strand, within OKGIIK_04935 at 1072.432 kb on + strand, within OKGIIK_04935 at 1072.433 kb on - strand, within OKGIIK_04935 at 1072.457 kb on + strand, within OKGIIK_04935 at 1072.492 kb on + strand, within OKGIIK_04935 at 1072.492 kb on + strand, within OKGIIK_04935 at 1072.492 kb on + strand, within OKGIIK_04935 at 1072.492 kb on + strand, within OKGIIK_04935 at 1072.492 kb on + strand, within OKGIIK_04935 at 1072.492 kb on + strand, within OKGIIK_04935 at 1072.493 kb on - strand, within OKGIIK_04935 at 1072.493 kb on - strand, within OKGIIK_04935 at 1072.493 kb on - strand, within OKGIIK_04935 at 1072.493 kb on - strand, within OKGIIK_04935 at 1072.493 kb on - strand, within OKGIIK_04935 at 1072.493 kb on - strand, within OKGIIK_04935 at 1072.493 kb on - strand, within OKGIIK_04935 at 1072.493 kb on - strand, within OKGIIK_04935 at 1072.534 kb on + strand, within OKGIIK_04935 at 1072.535 kb on - strand, within OKGIIK_04935 at 1072.535 kb on - strand, within OKGIIK_04935 at 1072.535 kb on - strand, within OKGIIK_04935 at 1072.691 kb on - strand at 1072.691 kb on - strand at 1072.691 kb on - strand at 1072.691 kb on - strand at 1072.691 kb on - strand at 1072.691 kb on - strand at 1072.691 kb on - strand at 1072.691 kb on - strand at 1072.835 kb on + strand at 1072.835 kb on + strand at 1072.912 kb on + strand at 1072.912 kb on + strand at 1072.912 kb on + strand at 1072.913 kb on - strand at 1072.913 kb on - strand at 1072.913 kb on - strand at 1072.962 kb on + strand at 1072.962 kb on + strand at 1072.962 kb on + strand at 1072.963 kb on - strand at 1072.963 kb on - strand at 1072.963 kb on - strand at 1072.963 kb on - strand at 1072.963 kb on - strand at 1073.248 kb on + strand, within yerC at 1073.248 kb on + strand, within yerC at 1073.248 kb on + strand, within yerC at 1073.249 kb on - strand, within yerC at 1073.249 kb on - strand, within yerC at 1073.273 kb on - strand, within yerC at 1073.716 kb on - strand, within hisG at 1073.716 kb on - strand, within hisG
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES pH 4 remove 1,072,036 + OKGIIK_04935 0.34 -1.4 1,072,037 - OKGIIK_04935 0.34 +0.3 1,072,037 - OKGIIK_04935 0.34 +0.5 1,072,037 - OKGIIK_04935 0.34 +0.3 1,072,037 - OKGIIK_04935 0.34 +0.6 1,072,037 - OKGIIK_04935 0.34 +0.3 1,072,037 - OKGIIK_04935 0.34 -0.5 1,072,058 - OKGIIK_04935 0.36 -0.3 1,072,058 - OKGIIK_04935 0.36 -0.4 1,072,070 - OKGIIK_04935 0.37 -1.1 1,072,070 - OKGIIK_04935 0.37 +1.3 1,072,327 + OKGIIK_04935 0.62 -0.1 1,072,327 + OKGIIK_04935 0.62 -0.3 1,072,328 - OKGIIK_04935 0.62 +0.6 1,072,328 - OKGIIK_04935 0.62 +0.3 1,072,328 - OKGIIK_04935 0.62 +2.0 1,072,354 + OKGIIK_04935 0.65 -1.3 1,072,369 + OKGIIK_04935 0.66 -1.5 1,072,370 - OKGIIK_04935 0.66 +0.3 1,072,370 - OKGIIK_04935 0.66 -2.6 1,072,370 - OKGIIK_04935 0.66 -1.4 1,072,432 + OKGIIK_04935 0.73 -0.8 1,072,433 - OKGIIK_04935 0.73 -0.7 1,072,457 + OKGIIK_04935 0.75 -0.4 1,072,492 + OKGIIK_04935 0.79 +0.0 1,072,492 + OKGIIK_04935 0.79 -2.1 1,072,492 + OKGIIK_04935 0.79 +0.5 1,072,492 + OKGIIK_04935 0.79 +0.6 1,072,492 + OKGIIK_04935 0.79 -0.7 1,072,492 + OKGIIK_04935 0.79 +0.2 1,072,493 - OKGIIK_04935 0.79 +0.5 1,072,493 - OKGIIK_04935 0.79 -2.7 1,072,493 - OKGIIK_04935 0.79 +0.2 1,072,493 - OKGIIK_04935 0.79 -0.5 1,072,493 - OKGIIK_04935 0.79 +1.3 1,072,493 - OKGIIK_04935 0.79 -0.1 1,072,493 - OKGIIK_04935 0.79 -0.4 1,072,493 - OKGIIK_04935 0.79 +0.3 1,072,534 + OKGIIK_04935 0.83 +1.3 1,072,535 - OKGIIK_04935 0.83 +0.6 1,072,535 - OKGIIK_04935 0.83 -0.4 1,072,535 - OKGIIK_04935 0.83 -0.8 1,072,691 - +1.2 1,072,691 - +0.2 1,072,691 - -0.8 1,072,691 - -0.1 1,072,691 - +0.3 1,072,691 - -0.7 1,072,691 - -0.8 1,072,691 - -2.1 1,072,835 + +0.0 1,072,835 + +0.3 1,072,912 + -0.3 1,072,912 + -1.8 1,072,912 + -1.3 1,072,913 - -0.8 1,072,913 - -0.5 1,072,913 - -1.8 1,072,962 + -0.4 1,072,962 + +0.0 1,072,962 + -2.2 1,072,963 - +0.7 1,072,963 - -0.1 1,072,963 - +0.1 1,072,963 - -0.5 1,072,963 - +1.6 1,073,248 + yerC OKGIIK_04940 0.74 +0.6 1,073,248 + yerC OKGIIK_04940 0.74 +2.5 1,073,248 + yerC OKGIIK_04940 0.74 +0.3 1,073,249 - yerC OKGIIK_04940 0.74 +1.8 1,073,249 - yerC OKGIIK_04940 0.74 +0.0 1,073,273 - yerC OKGIIK_04940 0.82 -1.8 1,073,716 - hisG OKGIIK_04945 0.43 +0.0 1,073,716 - hisG OKGIIK_04945 0.43 -0.7
Or see this region's nucleotide sequence