Experiment: R2A_PIPES pH 4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pcnB and folK overlap by 4 nucleotides folK and panB are separated by 55 nucleotides panB and panC are separated by 16 nucleotides panC and panD are separated by 102 nucleotides
OKGIIK_03875: pcnB - tRNA nucleotidyltransferase/poly(A) polymerase, at 851,396 to 852,745
pcnB
OKGIIK_03880: folK - 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase, at 852,742 to 853,221
folK
OKGIIK_03885: panB - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 853,277 to 854,107
panB
OKGIIK_03890: panC - pantoate--beta-alanine ligase, at 854,124 to 854,969
panC
OKGIIK_03895: panD - aspartate 1-decarboxylase, at 855,072 to 855,464
panD
Position (kb) 853
854
855 Strain fitness (log2 ratio) -1
0
1
2
3 at 852.688 kb on + strand at 852.688 kb on + strand at 853.280 kb on + strand at 853.280 kb on + strand at 853.280 kb on + strand at 853.281 kb on - strand at 853.281 kb on - strand at 853.380 kb on - strand, within panB at 853.498 kb on + strand, within panB at 853.499 kb on - strand, within panB at 853.499 kb on - strand, within panB at 853.499 kb on - strand, within panB at 853.499 kb on - strand, within panB at 854.134 kb on + strand at 854.134 kb on + strand at 854.134 kb on + strand at 854.135 kb on - strand at 854.135 kb on - strand at 854.135 kb on - strand at 854.135 kb on - strand at 854.135 kb on - strand at 854.325 kb on + strand, within panC at 854.325 kb on + strand, within panC at 854.326 kb on - strand, within panC at 854.418 kb on + strand, within panC at 854.418 kb on + strand, within panC at 854.698 kb on - strand, within panC at 854.980 kb on + strand at 854.981 kb on - strand at 854.995 kb on - strand at 855.065 kb on + strand at 855.065 kb on + strand at 855.066 kb on - strand at 855.066 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES pH 4 remove 852,688 + -0.9 852,688 + +1.4 853,280 + +2.1 853,280 + +0.8 853,280 + -0.5 853,281 - +1.4 853,281 - +2.3 853,380 - panB OKGIIK_03885 0.12 +2.4 853,498 + panB OKGIIK_03885 0.27 +1.4 853,499 - panB OKGIIK_03885 0.27 +0.1 853,499 - panB OKGIIK_03885 0.27 +2.8 853,499 - panB OKGIIK_03885 0.27 +0.4 853,499 - panB OKGIIK_03885 0.27 +2.0 854,134 + +1.2 854,134 + +0.4 854,134 + +0.4 854,135 - +0.9 854,135 - +0.1 854,135 - -0.8 854,135 - -0.2 854,135 - +1.4 854,325 + panC OKGIIK_03890 0.24 +1.4 854,325 + panC OKGIIK_03890 0.24 +1.0 854,326 - panC OKGIIK_03890 0.24 -0.4 854,418 + panC OKGIIK_03890 0.35 +0.4 854,418 + panC OKGIIK_03890 0.35 +1.3 854,698 - panC OKGIIK_03890 0.68 +0.6 854,980 + -0.4 854,981 - +0.4 854,995 - -1.3 855,065 + +3.1 855,065 + -0.2 855,066 - +0.9 855,066 - +0.8
Or see this region's nucleotide sequence