Experiment: R2A_PIPES pH 4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt trxC and OKGIIK_03475 are separated by 150 nucleotides OKGIIK_03475 and msrB are separated by 10 nucleotides msrB and ccdA are separated by 100 nucleotides
OKGIIK_03470: trxC - thioredoxin TrxC, at 772,882 to 773,325
trxC
OKGIIK_03475: OKGIIK_03475 - hypothetical protein, at 773,476 to 773,889
_03475
OKGIIK_03480: msrB - peptide-methionine (R)-S-oxide reductase, at 773,900 to 774,499
msrB
OKGIIK_03485: ccdA - cytochrome C biogenesis protein, at 774,600 to 776,321
ccdA
Position (kb) 773
774
775 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 773.319 kb on + strand at 773.319 kb on + strand at 773.319 kb on + strand at 773.319 kb on + strand at 773.319 kb on + strand at 773.319 kb on + strand at 773.319 kb on + strand at 773.319 kb on + strand at 773.319 kb on + strand at 773.319 kb on + strand at 773.319 kb on + strand at 773.319 kb on + strand at 773.320 kb on - strand at 773.320 kb on - strand at 773.320 kb on - strand at 773.320 kb on - strand at 773.320 kb on - strand at 773.320 kb on - strand at 773.320 kb on - strand at 773.320 kb on - strand at 773.320 kb on - strand at 773.320 kb on - strand at 773.320 kb on - strand at 773.320 kb on - strand at 773.320 kb on - strand at 773.320 kb on - strand at 773.320 kb on - strand at 773.502 kb on - strand at 773.502 kb on - strand at 773.502 kb on - strand at 773.502 kb on - strand at 773.502 kb on - strand at 773.502 kb on - strand at 773.502 kb on - strand at 773.674 kb on + strand, within OKGIIK_03475 at 773.674 kb on + strand, within OKGIIK_03475 at 773.674 kb on + strand, within OKGIIK_03475 at 773.674 kb on + strand, within OKGIIK_03475 at 773.674 kb on + strand, within OKGIIK_03475 at 773.674 kb on + strand, within OKGIIK_03475 at 773.675 kb on - strand, within OKGIIK_03475 at 773.675 kb on - strand, within OKGIIK_03475 at 773.743 kb on + strand, within OKGIIK_03475 at 773.743 kb on + strand, within OKGIIK_03475 at 773.744 kb on - strand, within OKGIIK_03475 at 773.749 kb on + strand, within OKGIIK_03475 at 773.749 kb on + strand, within OKGIIK_03475 at 773.749 kb on + strand, within OKGIIK_03475 at 773.749 kb on + strand, within OKGIIK_03475 at 773.749 kb on + strand, within OKGIIK_03475 at 773.749 kb on + strand, within OKGIIK_03475 at 773.749 kb on + strand, within OKGIIK_03475 at 773.749 kb on + strand, within OKGIIK_03475 at 773.749 kb on + strand, within OKGIIK_03475 at 773.749 kb on + strand, within OKGIIK_03475 at 773.749 kb on + strand, within OKGIIK_03475 at 773.749 kb on + strand, within OKGIIK_03475 at 773.749 kb on + strand, within OKGIIK_03475 at 773.749 kb on + strand, within OKGIIK_03475 at 773.749 kb on + strand, within OKGIIK_03475 at 773.749 kb on + strand, within OKGIIK_03475 at 773.749 kb on + strand, within OKGIIK_03475 at 773.749 kb on + strand, within OKGIIK_03475 at 773.749 kb on + strand, within OKGIIK_03475 at 773.749 kb on + strand, within OKGIIK_03475 at 773.749 kb on + strand, within OKGIIK_03475 at 773.749 kb on + strand, within OKGIIK_03475 at 773.749 kb on + strand, within OKGIIK_03475 at 773.750 kb on - strand, within OKGIIK_03475 at 773.750 kb on - strand, within OKGIIK_03475 at 773.750 kb on - strand, within OKGIIK_03475 at 773.750 kb on - strand, within OKGIIK_03475 at 773.750 kb on - strand, within OKGIIK_03475 at 773.750 kb on - strand, within OKGIIK_03475 at 773.750 kb on - strand, within OKGIIK_03475 at 773.750 kb on - strand, within OKGIIK_03475 at 773.750 kb on - strand, within OKGIIK_03475 at 773.750 kb on - strand, within OKGIIK_03475 at 773.750 kb on - strand, within OKGIIK_03475 at 773.750 kb on - strand, within OKGIIK_03475 at 773.750 kb on - strand, within OKGIIK_03475 at 773.750 kb on - strand, within OKGIIK_03475 at 773.750 kb on - strand, within OKGIIK_03475 at 773.750 kb on - strand, within OKGIIK_03475 at 773.750 kb on - strand, within OKGIIK_03475 at 773.750 kb on - strand, within OKGIIK_03475 at 773.750 kb on - strand, within OKGIIK_03475 at 773.750 kb on - strand, within OKGIIK_03475 at 773.750 kb on - strand, within OKGIIK_03475 at 773.750 kb on - strand, within OKGIIK_03475 at 773.750 kb on - strand, within OKGIIK_03475 at 773.750 kb on - strand, within OKGIIK_03475 at 773.750 kb on - strand, within OKGIIK_03475 at 773.750 kb on - strand, within OKGIIK_03475 at 773.782 kb on + strand, within OKGIIK_03475 at 773.782 kb on + strand, within OKGIIK_03475 at 773.782 kb on + strand, within OKGIIK_03475 at 773.782 kb on + strand, within OKGIIK_03475 at 773.782 kb on + strand, within OKGIIK_03475 at 773.782 kb on + strand, within OKGIIK_03475 at 773.782 kb on + strand, within OKGIIK_03475 at 773.782 kb on + strand, within OKGIIK_03475 at 773.782 kb on + strand, within OKGIIK_03475 at 773.782 kb on + strand, within OKGIIK_03475 at 773.782 kb on + strand, within OKGIIK_03475 at 773.782 kb on + strand, within OKGIIK_03475 at 773.783 kb on - strand, within OKGIIK_03475 at 773.783 kb on - strand, within OKGIIK_03475 at 773.783 kb on - strand, within OKGIIK_03475 at 773.783 kb on - strand, within OKGIIK_03475 at 773.783 kb on - strand, within OKGIIK_03475 at 773.840 kb on - strand, within OKGIIK_03475 at 773.946 kb on + strand at 773.947 kb on - strand at 774.135 kb on + strand, within msrB at 774.135 kb on + strand, within msrB at 774.135 kb on + strand, within msrB at 774.135 kb on + strand, within msrB at 774.135 kb on + strand, within msrB at 774.136 kb on - strand, within msrB at 774.136 kb on - strand, within msrB at 774.136 kb on - strand, within msrB at 774.136 kb on - strand, within msrB at 774.136 kb on - strand, within msrB at 774.136 kb on - strand, within msrB at 774.240 kb on + strand, within msrB at 774.240 kb on + strand, within msrB at 774.240 kb on + strand, within msrB at 774.240 kb on + strand, within msrB at 774.240 kb on + strand, within msrB at 774.241 kb on - strand, within msrB at 774.241 kb on - strand, within msrB at 774.241 kb on - strand, within msrB at 774.588 kb on + strand at 774.588 kb on + strand at 774.619 kb on + strand at 774.619 kb on + strand at 774.619 kb on + strand at 774.619 kb on + strand at 774.620 kb on - strand at 774.697 kb on + strand at 774.697 kb on + strand at 774.697 kb on + strand at 774.697 kb on + strand at 774.856 kb on + strand, within ccdA at 774.961 kb on + strand, within ccdA at 774.961 kb on + strand, within ccdA at 774.962 kb on - strand, within ccdA at 775.027 kb on + strand, within ccdA at 775.027 kb on + strand, within ccdA at 775.027 kb on + strand, within ccdA at 775.027 kb on + strand, within ccdA at 775.027 kb on + strand, within ccdA at 775.027 kb on + strand, within ccdA at 775.028 kb on - strand, within ccdA at 775.028 kb on - strand, within ccdA at 775.028 kb on - strand, within ccdA at 775.028 kb on - strand, within ccdA at 775.028 kb on - strand, within ccdA at 775.028 kb on - strand, within ccdA at 775.096 kb on + strand, within ccdA at 775.096 kb on + strand, within ccdA at 775.096 kb on + strand, within ccdA at 775.219 kb on + strand, within ccdA at 775.219 kb on + strand, within ccdA at 775.219 kb on + strand, within ccdA at 775.219 kb on + strand, within ccdA at 775.219 kb on + strand, within ccdA at 775.219 kb on + strand, within ccdA at 775.219 kb on + strand, within ccdA at 775.219 kb on + strand, within ccdA at 775.219 kb on + strand, within ccdA at 775.219 kb on + strand, within ccdA at 775.220 kb on - strand, within ccdA at 775.220 kb on - strand, within ccdA at 775.220 kb on - strand, within ccdA at 775.220 kb on - strand, within ccdA at 775.220 kb on - strand, within ccdA at 775.234 kb on + strand, within ccdA at 775.234 kb on + strand, within ccdA at 775.235 kb on - strand, within ccdA at 775.264 kb on + strand, within ccdA at 775.264 kb on + strand, within ccdA at 775.265 kb on - strand, within ccdA at 775.393 kb on + strand, within ccdA at 775.393 kb on + strand, within ccdA at 775.393 kb on + strand, within ccdA at 775.393 kb on + strand, within ccdA at 775.393 kb on + strand, within ccdA at 775.393 kb on + strand, within ccdA at 775.393 kb on + strand, within ccdA at 775.393 kb on + strand, within ccdA at 775.393 kb on + strand, within ccdA at 775.393 kb on + strand, within ccdA at 775.393 kb on + strand, within ccdA at 775.393 kb on + strand, within ccdA at 775.393 kb on + strand, within ccdA at 775.394 kb on - strand, within ccdA at 775.394 kb on - strand, within ccdA at 775.394 kb on - strand, within ccdA at 775.394 kb on - strand, within ccdA at 775.394 kb on - strand, within ccdA at 775.394 kb on - strand, within ccdA at 775.394 kb on - strand, within ccdA at 775.394 kb on - strand, within ccdA at 775.394 kb on - strand, within ccdA at 775.394 kb on - strand, within ccdA at 775.394 kb on - strand, within ccdA at 775.417 kb on + strand, within ccdA at 775.417 kb on + strand, within ccdA at 775.417 kb on + strand, within ccdA at 775.417 kb on + strand, within ccdA at 775.417 kb on + strand, within ccdA at 775.418 kb on - strand, within ccdA at 775.418 kb on - strand, within ccdA at 775.450 kb on + strand, within ccdA at 775.450 kb on + strand, within ccdA at 775.450 kb on + strand, within ccdA at 775.451 kb on - strand, within ccdA at 775.451 kb on - strand, within ccdA at 775.451 kb on - strand, within ccdA at 775.451 kb on - strand, within ccdA at 775.451 kb on - strand, within ccdA at 775.451 kb on - strand, within ccdA at 775.451 kb on - strand, within ccdA
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES pH 4 remove 773,319 + -2.9 773,319 + +0.4 773,319 + -1.1 773,319 + +0.4 773,319 + +1.2 773,319 + -0.5 773,319 + +0.4 773,319 + -1.0 773,319 + -1.0 773,319 + +0.4 773,319 + -0.5 773,319 + -0.4 773,320 - -0.1 773,320 - +0.4 773,320 - -1.0 773,320 - -1.4 773,320 - -0.5 773,320 - -1.0 773,320 - +0.5 773,320 - -0.2 773,320 - +0.9 773,320 - -0.6 773,320 - +0.4 773,320 - -1.3 773,320 - -0.3 773,320 - -0.1 773,320 - -0.5 773,502 - -1.0 773,502 - -0.6 773,502 - +2.4 773,502 - +0.0 773,502 - +1.7 773,502 - +0.9 773,502 - -1.2 773,674 + OKGIIK_03475 0.48 +0.5 773,674 + OKGIIK_03475 0.48 +0.2 773,674 + OKGIIK_03475 0.48 +0.4 773,674 + OKGIIK_03475 0.48 -1.2 773,674 + OKGIIK_03475 0.48 -0.9 773,674 + OKGIIK_03475 0.48 -0.5 773,675 - OKGIIK_03475 0.48 -0.6 773,675 - OKGIIK_03475 0.48 -0.6 773,743 + OKGIIK_03475 0.64 +1.2 773,743 + OKGIIK_03475 0.64 +0.8 773,744 - OKGIIK_03475 0.65 -0.3 773,749 + OKGIIK_03475 0.66 +1.6 773,749 + OKGIIK_03475 0.66 -0.2 773,749 + OKGIIK_03475 0.66 +0.4 773,749 + OKGIIK_03475 0.66 -1.4 773,749 + OKGIIK_03475 0.66 -0.2 773,749 + OKGIIK_03475 0.66 -1.6 773,749 + OKGIIK_03475 0.66 +0.9 773,749 + OKGIIK_03475 0.66 -2.6 773,749 + OKGIIK_03475 0.66 -0.6 773,749 + OKGIIK_03475 0.66 +0.8 773,749 + OKGIIK_03475 0.66 -0.2 773,749 + OKGIIK_03475 0.66 -1.0 773,749 + OKGIIK_03475 0.66 +1.5 773,749 + OKGIIK_03475 0.66 +0.0 773,749 + OKGIIK_03475 0.66 -1.2 773,749 + OKGIIK_03475 0.66 -0.7 773,749 + OKGIIK_03475 0.66 +0.5 773,749 + OKGIIK_03475 0.66 +0.8 773,749 + OKGIIK_03475 0.66 -0.6 773,749 + OKGIIK_03475 0.66 -1.5 773,749 + OKGIIK_03475 0.66 +0.4 773,749 + OKGIIK_03475 0.66 -1.8 773,749 + OKGIIK_03475 0.66 -0.2 773,750 - OKGIIK_03475 0.66 -0.8 773,750 - OKGIIK_03475 0.66 -1.0 773,750 - OKGIIK_03475 0.66 +1.0 773,750 - OKGIIK_03475 0.66 +0.0 773,750 - OKGIIK_03475 0.66 +0.4 773,750 - OKGIIK_03475 0.66 +0.3 773,750 - OKGIIK_03475 0.66 -0.6 773,750 - OKGIIK_03475 0.66 +0.3 773,750 - OKGIIK_03475 0.66 -1.5 773,750 - OKGIIK_03475 0.66 -0.8 773,750 - OKGIIK_03475 0.66 -0.5 773,750 - OKGIIK_03475 0.66 -1.6 773,750 - OKGIIK_03475 0.66 -0.2 773,750 - OKGIIK_03475 0.66 -2.1 773,750 - OKGIIK_03475 0.66 -3.2 773,750 - OKGIIK_03475 0.66 +0.4 773,750 - OKGIIK_03475 0.66 -0.2 773,750 - OKGIIK_03475 0.66 +0.0 773,750 - OKGIIK_03475 0.66 +0.2 773,750 - OKGIIK_03475 0.66 +1.2 773,750 - OKGIIK_03475 0.66 +0.5 773,750 - OKGIIK_03475 0.66 -0.7 773,750 - OKGIIK_03475 0.66 -0.6 773,750 - OKGIIK_03475 0.66 -0.5 773,750 - OKGIIK_03475 0.66 +0.5 773,750 - OKGIIK_03475 0.66 -0.7 773,782 + OKGIIK_03475 0.74 -0.1 773,782 + OKGIIK_03475 0.74 -2.5 773,782 + OKGIIK_03475 0.74 +0.2 773,782 + OKGIIK_03475 0.74 -1.1 773,782 + OKGIIK_03475 0.74 +1.4 773,782 + OKGIIK_03475 0.74 -1.1 773,782 + OKGIIK_03475 0.74 -0.3 773,782 + OKGIIK_03475 0.74 -0.5 773,782 + OKGIIK_03475 0.74 -0.2 773,782 + OKGIIK_03475 0.74 +1.0 773,782 + OKGIIK_03475 0.74 -0.2 773,782 + OKGIIK_03475 0.74 -0.6 773,783 - OKGIIK_03475 0.74 +0.5 773,783 - OKGIIK_03475 0.74 -0.6 773,783 - OKGIIK_03475 0.74 -0.7 773,783 - OKGIIK_03475 0.74 +0.0 773,783 - OKGIIK_03475 0.74 -0.5 773,840 - OKGIIK_03475 0.88 -0.5 773,946 + -0.1 773,947 - -0.8 774,135 + msrB OKGIIK_03480 0.39 +0.0 774,135 + msrB OKGIIK_03480 0.39 +0.8 774,135 + msrB OKGIIK_03480 0.39 +0.4 774,135 + msrB OKGIIK_03480 0.39 -1.0 774,135 + msrB OKGIIK_03480 0.39 +1.0 774,136 - msrB OKGIIK_03480 0.39 +1.0 774,136 - msrB OKGIIK_03480 0.39 -0.6 774,136 - msrB OKGIIK_03480 0.39 +1.0 774,136 - msrB OKGIIK_03480 0.39 -1.0 774,136 - msrB OKGIIK_03480 0.39 +0.2 774,136 - msrB OKGIIK_03480 0.39 +1.5 774,240 + msrB OKGIIK_03480 0.57 +0.4 774,240 + msrB OKGIIK_03480 0.57 -0.8 774,240 + msrB OKGIIK_03480 0.57 -2.0 774,240 + msrB OKGIIK_03480 0.57 +0.1 774,240 + msrB OKGIIK_03480 0.57 +1.0 774,241 - msrB OKGIIK_03480 0.57 +0.9 774,241 - msrB OKGIIK_03480 0.57 +1.0 774,241 - msrB OKGIIK_03480 0.57 +1.2 774,588 + +1.7 774,588 + -0.5 774,619 + +0.4 774,619 + -1.0 774,619 + -0.5 774,619 + +0.6 774,620 - -0.0 774,697 + +1.2 774,697 + +1.9 774,697 + -0.8 774,697 + +1.4 774,856 + ccdA OKGIIK_03485 0.15 -0.1 774,961 + ccdA OKGIIK_03485 0.21 -1.1 774,961 + ccdA OKGIIK_03485 0.21 -0.6 774,962 - ccdA OKGIIK_03485 0.21 -0.6 775,027 + ccdA OKGIIK_03485 0.25 -1.0 775,027 + ccdA OKGIIK_03485 0.25 +0.8 775,027 + ccdA OKGIIK_03485 0.25 +1.0 775,027 + ccdA OKGIIK_03485 0.25 -1.8 775,027 + ccdA OKGIIK_03485 0.25 -0.4 775,027 + ccdA OKGIIK_03485 0.25 -0.7 775,028 - ccdA OKGIIK_03485 0.25 -0.2 775,028 - ccdA OKGIIK_03485 0.25 +0.4 775,028 - ccdA OKGIIK_03485 0.25 -2.8 775,028 - ccdA OKGIIK_03485 0.25 +0.5 775,028 - ccdA OKGIIK_03485 0.25 +0.2 775,028 - ccdA OKGIIK_03485 0.25 +0.4 775,096 + ccdA OKGIIK_03485 0.29 +0.4 775,096 + ccdA OKGIIK_03485 0.29 -1.1 775,096 + ccdA OKGIIK_03485 0.29 +1.2 775,219 + ccdA OKGIIK_03485 0.36 -0.5 775,219 + ccdA OKGIIK_03485 0.36 +0.8 775,219 + ccdA OKGIIK_03485 0.36 +0.2 775,219 + ccdA OKGIIK_03485 0.36 -1.5 775,219 + ccdA OKGIIK_03485 0.36 +0.9 775,219 + ccdA OKGIIK_03485 0.36 -1.2 775,219 + ccdA OKGIIK_03485 0.36 -0.5 775,219 + ccdA OKGIIK_03485 0.36 -1.1 775,219 + ccdA OKGIIK_03485 0.36 -0.5 775,219 + ccdA OKGIIK_03485 0.36 +1.0 775,220 - ccdA OKGIIK_03485 0.36 -0.8 775,220 - ccdA OKGIIK_03485 0.36 +0.8 775,220 - ccdA OKGIIK_03485 0.36 +0.5 775,220 - ccdA OKGIIK_03485 0.36 +1.4 775,220 - ccdA OKGIIK_03485 0.36 +1.4 775,234 + ccdA OKGIIK_03485 0.37 -0.6 775,234 + ccdA OKGIIK_03485 0.37 -1.6 775,235 - ccdA OKGIIK_03485 0.37 -2.0 775,264 + ccdA OKGIIK_03485 0.39 +1.0 775,264 + ccdA OKGIIK_03485 0.39 +1.9 775,265 - ccdA OKGIIK_03485 0.39 -0.5 775,393 + ccdA OKGIIK_03485 0.46 +0.0 775,393 + ccdA OKGIIK_03485 0.46 -2.2 775,393 + ccdA OKGIIK_03485 0.46 +0.4 775,393 + ccdA OKGIIK_03485 0.46 -0.6 775,393 + ccdA OKGIIK_03485 0.46 -1.2 775,393 + ccdA OKGIIK_03485 0.46 +0.0 775,393 + ccdA OKGIIK_03485 0.46 -3.1 775,393 + ccdA OKGIIK_03485 0.46 +0.0 775,393 + ccdA OKGIIK_03485 0.46 -0.2 775,393 + ccdA OKGIIK_03485 0.46 +1.6 775,393 + ccdA OKGIIK_03485 0.46 -0.6 775,393 + ccdA OKGIIK_03485 0.46 +1.3 775,393 + ccdA OKGIIK_03485 0.46 -0.5 775,394 - ccdA OKGIIK_03485 0.46 -0.5 775,394 - ccdA OKGIIK_03485 0.46 -1.0 775,394 - ccdA OKGIIK_03485 0.46 +0.0 775,394 - ccdA OKGIIK_03485 0.46 -0.2 775,394 - ccdA OKGIIK_03485 0.46 -0.1 775,394 - ccdA OKGIIK_03485 0.46 -0.3 775,394 - ccdA OKGIIK_03485 0.46 +1.8 775,394 - ccdA OKGIIK_03485 0.46 +0.0 775,394 - ccdA OKGIIK_03485 0.46 +0.4 775,394 - ccdA OKGIIK_03485 0.46 -1.2 775,394 - ccdA OKGIIK_03485 0.46 -1.1 775,417 + ccdA OKGIIK_03485 0.47 +0.0 775,417 + ccdA OKGIIK_03485 0.47 +1.0 775,417 + ccdA OKGIIK_03485 0.47 -1.0 775,417 + ccdA OKGIIK_03485 0.47 +0.4 775,417 + ccdA OKGIIK_03485 0.47 -0.5 775,418 - ccdA OKGIIK_03485 0.48 -0.6 775,418 - ccdA OKGIIK_03485 0.48 +2.4 775,450 + ccdA OKGIIK_03485 0.49 -1.1 775,450 + ccdA OKGIIK_03485 0.49 +0.8 775,450 + ccdA OKGIIK_03485 0.49 -0.1 775,451 - ccdA OKGIIK_03485 0.49 -1.0 775,451 - ccdA OKGIIK_03485 0.49 -1.3 775,451 - ccdA OKGIIK_03485 0.49 -0.2 775,451 - ccdA OKGIIK_03485 0.49 +0.2 775,451 - ccdA OKGIIK_03485 0.49 -0.8 775,451 - ccdA OKGIIK_03485 0.49 -1.7 775,451 - ccdA OKGIIK_03485 0.49 +0.5
Or see this region's nucleotide sequence