Experiment: R2A_PIPES pH 4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt creD and tsaB overlap by 4 nucleotides tsaB and OKGIIK_02225 are separated by 170 nucleotides OKGIIK_02225 and lysR are separated by 87 nucleotides
OKGIIK_02215: creD - cell envelope integrity protein CreD, at 520,791 to 522,140
creD
OKGIIK_02220: tsaB - tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB, at 522,137 to 522,829
tsaB
OKGIIK_02225: OKGIIK_02225 - Conserved hypothetical integral membrane protein, at 523,000 to 524,037
_02225
OKGIIK_02230: lysR - LysR family transcriptional regulator, at 524,125 to 525,000
lysR
Position (kb) 522
523
524
525 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 522.006 kb on + strand at 522.006 kb on + strand at 522.006 kb on + strand at 522.006 kb on + strand at 522.006 kb on + strand at 522.006 kb on + strand at 522.007 kb on - strand at 522.007 kb on - strand at 522.007 kb on - strand at 522.007 kb on - strand at 522.060 kb on + strand at 522.060 kb on + strand at 522.060 kb on + strand at 522.060 kb on + strand at 522.060 kb on + strand at 522.060 kb on + strand at 522.060 kb on + strand at 522.060 kb on + strand at 522.060 kb on + strand at 522.060 kb on + strand at 522.060 kb on + strand at 522.060 kb on + strand at 522.060 kb on + strand at 522.060 kb on + strand at 522.060 kb on + strand at 522.060 kb on + strand at 522.060 kb on + strand at 522.060 kb on + strand at 522.060 kb on + strand at 522.060 kb on + strand at 522.060 kb on + strand at 522.060 kb on + strand at 522.060 kb on + strand at 522.060 kb on + strand at 522.060 kb on + strand at 522.060 kb on + strand at 522.061 kb on - strand at 522.061 kb on - strand at 522.061 kb on - strand at 522.061 kb on - strand at 522.061 kb on - strand at 522.061 kb on - strand at 522.061 kb on - strand at 522.061 kb on - strand at 522.061 kb on - strand at 522.061 kb on - strand at 522.061 kb on - strand at 522.061 kb on - strand at 522.061 kb on - strand at 522.061 kb on - strand at 522.061 kb on - strand at 522.061 kb on - strand at 522.061 kb on - strand at 522.061 kb on - strand at 522.061 kb on - strand at 522.061 kb on - strand at 522.061 kb on - strand at 522.061 kb on - strand at 522.061 kb on - strand at 522.061 kb on - strand at 522.061 kb on - strand at 522.061 kb on - strand at 522.061 kb on - strand at 522.061 kb on - strand at 522.061 kb on - strand at 522.061 kb on - strand at 522.061 kb on - strand at 522.061 kb on - strand at 522.061 kb on - strand at 522.061 kb on - strand at 522.084 kb on + strand at 522.084 kb on + strand at 522.084 kb on + strand at 522.084 kb on + strand at 522.084 kb on + strand at 522.085 kb on - strand at 522.085 kb on - strand at 522.085 kb on - strand at 522.085 kb on - strand at 522.085 kb on - strand at 522.090 kb on + strand at 522.090 kb on + strand at 522.090 kb on + strand at 522.090 kb on + strand at 522.090 kb on + strand at 522.091 kb on - strand at 522.091 kb on - strand at 522.091 kb on - strand at 522.091 kb on - strand at 522.091 kb on - strand at 522.091 kb on - strand at 522.091 kb on - strand at 522.091 kb on - strand at 522.091 kb on - strand at 522.122 kb on + strand at 522.123 kb on - strand at 522.801 kb on + strand at 522.832 kb on + strand at 522.832 kb on + strand at 522.832 kb on + strand at 522.832 kb on + strand at 522.832 kb on + strand at 522.832 kb on + strand at 522.833 kb on - strand at 522.833 kb on - strand at 522.833 kb on - strand at 522.833 kb on - strand at 522.833 kb on - strand at 522.833 kb on - strand at 522.833 kb on - strand at 522.833 kb on - strand at 522.833 kb on - strand at 522.833 kb on - strand at 522.833 kb on - strand at 522.833 kb on - strand at 522.833 kb on - strand at 522.955 kb on + strand at 522.955 kb on + strand at 522.955 kb on + strand at 522.955 kb on + strand at 522.955 kb on + strand at 522.955 kb on + strand at 522.955 kb on + strand at 522.955 kb on + strand at 522.955 kb on + strand at 522.955 kb on + strand at 522.955 kb on + strand at 522.955 kb on + strand at 522.955 kb on + strand at 522.955 kb on + strand at 522.955 kb on + strand at 522.955 kb on + strand at 522.955 kb on + strand at 522.956 kb on - strand at 522.956 kb on - strand at 522.956 kb on - strand at 522.956 kb on - strand at 522.956 kb on - strand at 522.956 kb on - strand at 522.956 kb on - strand at 522.956 kb on - strand at 522.956 kb on - strand at 522.956 kb on - strand at 522.956 kb on - strand at 522.956 kb on - strand at 522.956 kb on - strand at 522.956 kb on - strand at 522.956 kb on - strand at 522.956 kb on - strand at 522.956 kb on - strand at 522.956 kb on - strand at 522.956 kb on - strand at 523.392 kb on - strand, within OKGIIK_02225 at 523.412 kb on + strand, within OKGIIK_02225 at 523.413 kb on - strand, within OKGIIK_02225 at 523.413 kb on - strand, within OKGIIK_02225 at 523.413 kb on - strand, within OKGIIK_02225 at 523.413 kb on - strand, within OKGIIK_02225 at 523.413 kb on - strand, within OKGIIK_02225 at 523.413 kb on - strand, within OKGIIK_02225 at 523.413 kb on - strand, within OKGIIK_02225 at 523.850 kb on + strand, within OKGIIK_02225 at 523.850 kb on + strand, within OKGIIK_02225 at 524.017 kb on + strand at 524.017 kb on + strand at 524.017 kb on + strand at 524.017 kb on + strand at 524.018 kb on - strand at 524.097 kb on + strand at 524.097 kb on + strand at 524.098 kb on - strand at 524.098 kb on - strand at 524.098 kb on - strand at 524.099 kb on + strand at 524.099 kb on + strand at 524.099 kb on + strand at 524.100 kb on - strand at 524.100 kb on - strand at 524.100 kb on - strand at 524.100 kb on - strand at 524.100 kb on - strand at 524.100 kb on - strand at 524.100 kb on - strand at 524.100 kb on - strand at 524.100 kb on - strand at 524.111 kb on + strand at 524.111 kb on + strand at 524.112 kb on - strand at 524.112 kb on - strand at 524.112 kb on - strand at 524.112 kb on - strand at 524.112 kb on - strand at 524.112 kb on - strand at 524.112 kb on - strand at 524.113 kb on + strand at 524.113 kb on + strand at 524.114 kb on - strand at 524.114 kb on - strand at 524.114 kb on - strand at 524.114 kb on - strand at 524.114 kb on - strand at 524.114 kb on - strand at 524.114 kb on - strand at 524.114 kb on - strand at 524.114 kb on - strand at 524.118 kb on + strand at 524.118 kb on + strand at 524.119 kb on - strand at 524.119 kb on - strand at 524.119 kb on - strand at 524.119 kb on - strand at 524.119 kb on - strand at 524.119 kb on - strand at 524.430 kb on + strand, within lysR at 524.430 kb on + strand, within lysR at 524.430 kb on + strand, within lysR at 524.430 kb on + strand, within lysR at 524.430 kb on + strand, within lysR at 524.430 kb on + strand, within lysR at 524.430 kb on + strand, within lysR at 524.430 kb on + strand, within lysR at 524.430 kb on + strand, within lysR at 524.430 kb on + strand, within lysR at 524.430 kb on + strand, within lysR at 524.431 kb on - strand, within lysR at 524.431 kb on - strand, within lysR at 524.431 kb on - strand, within lysR at 524.431 kb on - strand, within lysR at 524.431 kb on - strand, within lysR at 524.431 kb on - strand, within lysR at 524.431 kb on - strand, within lysR at 524.431 kb on - strand, within lysR at 524.431 kb on - strand, within lysR at 524.431 kb on - strand, within lysR at 524.431 kb on - strand, within lysR at 524.431 kb on - strand, within lysR at 524.431 kb on - strand, within lysR at 524.443 kb on + strand, within lysR at 524.443 kb on + strand, within lysR at 524.443 kb on + strand, within lysR at 524.443 kb on + strand, within lysR at 524.443 kb on + strand, within lysR at 524.443 kb on + strand, within lysR at 524.443 kb on + strand, within lysR at 524.443 kb on + strand, within lysR at 524.443 kb on + strand, within lysR at 524.443 kb on + strand, within lysR at 524.444 kb on - strand, within lysR at 524.444 kb on - strand, within lysR at 524.444 kb on - strand, within lysR at 524.444 kb on - strand, within lysR at 524.444 kb on - strand, within lysR at 524.444 kb on - strand, within lysR at 524.444 kb on - strand, within lysR at 524.444 kb on - strand, within lysR at 524.444 kb on - strand, within lysR at 524.444 kb on - strand, within lysR at 524.444 kb on - strand, within lysR at 524.517 kb on + strand, within lysR at 524.959 kb on + strand at 524.959 kb on + strand at 524.959 kb on + strand at 524.959 kb on + strand at 524.960 kb on - strand at 524.960 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES pH 4 remove 522,006 + -1.4 522,006 + -0.4 522,006 + -2.0 522,006 + -0.4 522,006 + -0.3 522,006 + -1.8 522,007 - -1.4 522,007 - +0.6 522,007 - -0.8 522,007 - -0.3 522,060 + +0.6 522,060 + +0.1 522,060 + -1.9 522,060 + -1.4 522,060 + -2.8 522,060 + -1.9 522,060 + +0.6 522,060 + -1.0 522,060 + -0.8 522,060 + -0.4 522,060 + -0.4 522,060 + -1.5 522,060 + -0.8 522,060 + -1.4 522,060 + +1.3 522,060 + -0.4 522,060 + -0.4 522,060 + +1.6 522,060 + -0.4 522,060 + -1.3 522,060 + -2.0 522,060 + -2.8 522,060 + -1.6 522,060 + -2.9 522,060 + +1.6 522,060 + -0.0 522,061 - +0.4 522,061 - +0.6 522,061 - -1.1 522,061 - -1.4 522,061 - +0.6 522,061 - +0.8 522,061 - -0.8 522,061 - -1.8 522,061 - -0.4 522,061 - -1.4 522,061 - -1.1 522,061 - -1.1 522,061 - -0.6 522,061 - -0.8 522,061 - -0.6 522,061 - -1.0 522,061 - -0.4 522,061 - -1.0 522,061 - -1.3 522,061 - -1.9 522,061 - +0.2 522,061 - -1.0 522,061 - -0.8 522,061 - -0.8 522,061 - -1.0 522,061 - -1.6 522,061 - -1.0 522,061 - +0.4 522,061 - -0.4 522,061 - -0.8 522,061 - -1.9 522,061 - -0.4 522,061 - -0.9 522,061 - -1.0 522,084 + -2.8 522,084 + -1.4 522,084 + +1.0 522,084 + -1.4 522,084 + -1.9 522,085 - +0.7 522,085 - +0.8 522,085 - +0.1 522,085 - -0.3 522,085 - +0.2 522,090 + -0.8 522,090 + -0.4 522,090 + +0.3 522,090 + -0.0 522,090 + -2.1 522,091 - +0.7 522,091 - -0.4 522,091 - -0.8 522,091 - -1.2 522,091 - -1.4 522,091 - -1.4 522,091 - -0.1 522,091 - -1.4 522,091 - +1.6 522,122 + +0.1 522,123 - -1.2 522,801 + -1.0 522,832 + -1.4 522,832 + -0.4 522,832 + -0.9 522,832 + -1.4 522,832 + -0.2 522,832 + -2.4 522,833 - -2.0 522,833 - -0.6 522,833 - -1.6 522,833 - -0.9 522,833 - -1.6 522,833 - -1.6 522,833 - -1.0 522,833 - +1.6 522,833 - -1.0 522,833 - -1.6 522,833 - +0.1 522,833 - +0.2 522,833 - -0.5 522,955 + -0.4 522,955 + -2.3 522,955 + -0.4 522,955 + -0.1 522,955 + -1.1 522,955 + -2.5 522,955 + +0.5 522,955 + -0.8 522,955 + -0.4 522,955 + -0.7 522,955 + -2.3 522,955 + -1.8 522,955 + -1.6 522,955 + -0.4 522,955 + -0.8 522,955 + -2.5 522,955 + +0.4 522,956 - -1.6 522,956 - -1.0 522,956 - -0.1 522,956 - -0.4 522,956 - -1.9 522,956 - -0.4 522,956 - +0.6 522,956 - -0.4 522,956 - -0.0 522,956 - -0.7 522,956 - -1.1 522,956 - -1.8 522,956 - -0.9 522,956 - -0.8 522,956 - -0.4 522,956 - -0.4 522,956 - -0.8 522,956 - -3.9 522,956 - -1.3 523,392 - OKGIIK_02225 0.38 -1.1 523,412 + OKGIIK_02225 0.40 -0.0 523,413 - OKGIIK_02225 0.40 -1.6 523,413 - OKGIIK_02225 0.40 -1.0 523,413 - OKGIIK_02225 0.40 -1.1 523,413 - OKGIIK_02225 0.40 -1.8 523,413 - OKGIIK_02225 0.40 -0.8 523,413 - OKGIIK_02225 0.40 -0.4 523,413 - OKGIIK_02225 0.40 -0.4 523,850 + OKGIIK_02225 0.82 -0.8 523,850 + OKGIIK_02225 0.82 +1.2 524,017 + -2.1 524,017 + -0.0 524,017 + -0.6 524,017 + +1.2 524,018 - -0.9 524,097 + -2.3 524,097 + -2.1 524,098 - +0.9 524,098 - -0.6 524,098 - -1.4 524,099 + -1.3 524,099 + -0.4 524,099 + -1.3 524,100 - -1.0 524,100 - -1.0 524,100 - +0.2 524,100 - -1.9 524,100 - -1.7 524,100 - -1.0 524,100 - -2.4 524,100 - +1.1 524,100 - +1.1 524,111 + -1.3 524,111 + -1.6 524,112 - -1.4 524,112 - -0.8 524,112 - -1.1 524,112 - +0.6 524,112 - -1.0 524,112 - +0.6 524,112 - +0.1 524,113 + -1.0 524,113 + -1.4 524,114 - +0.6 524,114 - -1.0 524,114 - -0.6 524,114 - +0.6 524,114 - -0.9 524,114 - -0.1 524,114 - -0.4 524,114 - +0.1 524,114 - +0.6 524,118 + +0.6 524,118 + -0.8 524,119 - -2.1 524,119 - -0.8 524,119 - +0.6 524,119 - -2.8 524,119 - -0.8 524,119 - +0.8 524,430 + lysR OKGIIK_02230 0.35 -0.5 524,430 + lysR OKGIIK_02230 0.35 -0.3 524,430 + lysR OKGIIK_02230 0.35 -0.3 524,430 + lysR OKGIIK_02230 0.35 +0.6 524,430 + lysR OKGIIK_02230 0.35 +1.1 524,430 + lysR OKGIIK_02230 0.35 -0.8 524,430 + lysR OKGIIK_02230 0.35 -0.8 524,430 + lysR OKGIIK_02230 0.35 -1.4 524,430 + lysR OKGIIK_02230 0.35 -0.6 524,430 + lysR OKGIIK_02230 0.35 -1.4 524,430 + lysR OKGIIK_02230 0.35 -0.1 524,431 - lysR OKGIIK_02230 0.35 -1.4 524,431 - lysR OKGIIK_02230 0.35 -0.8 524,431 - lysR OKGIIK_02230 0.35 +0.8 524,431 - lysR OKGIIK_02230 0.35 -2.1 524,431 - lysR OKGIIK_02230 0.35 -2.9 524,431 - lysR OKGIIK_02230 0.35 +0.4 524,431 - lysR OKGIIK_02230 0.35 +0.8 524,431 - lysR OKGIIK_02230 0.35 -2.3 524,431 - lysR OKGIIK_02230 0.35 -0.6 524,431 - lysR OKGIIK_02230 0.35 +1.2 524,431 - lysR OKGIIK_02230 0.35 -0.5 524,431 - lysR OKGIIK_02230 0.35 -0.4 524,431 - lysR OKGIIK_02230 0.35 +0.6 524,443 + lysR OKGIIK_02230 0.36 -1.6 524,443 + lysR OKGIIK_02230 0.36 -0.8 524,443 + lysR OKGIIK_02230 0.36 -1.6 524,443 + lysR OKGIIK_02230 0.36 -2.4 524,443 + lysR OKGIIK_02230 0.36 -0.0 524,443 + lysR OKGIIK_02230 0.36 -1.7 524,443 + lysR OKGIIK_02230 0.36 -1.8 524,443 + lysR OKGIIK_02230 0.36 -0.8 524,443 + lysR OKGIIK_02230 0.36 +0.1 524,443 + lysR OKGIIK_02230 0.36 +1.1 524,444 - lysR OKGIIK_02230 0.36 -1.9 524,444 - lysR OKGIIK_02230 0.36 -1.1 524,444 - lysR OKGIIK_02230 0.36 -0.4 524,444 - lysR OKGIIK_02230 0.36 -1.3 524,444 - lysR OKGIIK_02230 0.36 -0.4 524,444 - lysR OKGIIK_02230 0.36 -0.4 524,444 - lysR OKGIIK_02230 0.36 -0.3 524,444 - lysR OKGIIK_02230 0.36 -1.9 524,444 - lysR OKGIIK_02230 0.36 -0.8 524,444 - lysR OKGIIK_02230 0.36 -1.9 524,444 - lysR OKGIIK_02230 0.36 -0.4 524,517 + lysR OKGIIK_02230 0.45 -0.4 524,959 + -1.4 524,959 + -0.4 524,959 + -1.3 524,959 + +0.8 524,960 - -1.0 524,960 - -2.9
Or see this region's nucleotide sequence