Experiment: R2A_PIPES pH 4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt aspS and OKGIIK_01825 are separated by 77 nucleotides OKGIIK_01825 and tACO1 are separated by 98 nucleotides tACO1 and ruvC are separated by 34 nucleotides ruvC and ruvA are separated by 25 nucleotides
OKGIIK_01820: aspS - aspartate--tRNA ligase, at 423,093 to 424,865
aspS
OKGIIK_01825: OKGIIK_01825 - AB hydrolase-1 domain-containing protein, at 424,943 to 425,578
_01825
OKGIIK_01830: tACO1 - YebC/PmpR family DNA-binding transcriptional regulator, at 425,677 to 426,411
tACO1
OKGIIK_01835: ruvC - crossover junction endodeoxyribonuclease RuvC, at 426,446 to 426,967
ruvC
OKGIIK_01840: ruvA - Holliday junction branch migration protein RuvA, at 426,993 to 427,589
ruvA
Position (kb) 425
426
427 Strain fitness (log2 ratio) -2
-1
0
1 at 425.051 kb on + strand, within OKGIIK_01825 at 425.051 kb on + strand, within OKGIIK_01825 at 425.051 kb on + strand, within OKGIIK_01825 at 425.051 kb on + strand, within OKGIIK_01825 at 425.051 kb on + strand, within OKGIIK_01825 at 425.052 kb on - strand, within OKGIIK_01825 at 425.052 kb on - strand, within OKGIIK_01825 at 425.052 kb on - strand, within OKGIIK_01825 at 425.682 kb on + strand at 425.682 kb on + strand at 425.683 kb on - strand at 425.926 kb on + strand, within tACO1 at 427.098 kb on + strand, within ruvA at 427.173 kb on + strand, within ruvA
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES pH 4 remove 425,051 + OKGIIK_01825 0.17 -1.1 425,051 + OKGIIK_01825 0.17 -0.3 425,051 + OKGIIK_01825 0.17 +1.3 425,051 + OKGIIK_01825 0.17 +0.2 425,051 + OKGIIK_01825 0.17 +0.7 425,052 - OKGIIK_01825 0.17 -1.9 425,052 - OKGIIK_01825 0.17 -1.6 425,052 - OKGIIK_01825 0.17 -0.6 425,682 + +1.3 425,682 + +1.1 425,683 - +1.2 425,926 + tACO1 OKGIIK_01830 0.34 +0.6 427,098 + ruvA OKGIIK_01840 0.18 -1.1 427,173 + ruvA OKGIIK_01840 0.30 -0.0
Or see this region's nucleotide sequence