Experiment: R2A_PIPES pH 4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cysK and OKGIIK_01505 are separated by 146 nucleotides OKGIIK_01505 and mET2 are separated by 348 nucleotides
OKGIIK_01500: cysK - cysteine synthase A, at 337,630 to 338,646
cysK
OKGIIK_01505: OKGIIK_01505 - peroxiredoxin, at 338,793 to 339,428
_01505
OKGIIK_01510: mET2 - homoserine O-succinyltransferase, at 339,777 to 340,808
mET2
Position (kb) 338
339
340 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 337.991 kb on + strand, within cysK at 337.991 kb on + strand, within cysK at 337.991 kb on + strand, within cysK at 337.991 kb on + strand, within cysK at 337.991 kb on + strand, within cysK at 337.991 kb on + strand, within cysK at 337.991 kb on + strand, within cysK at 337.991 kb on + strand, within cysK at 337.991 kb on + strand, within cysK at 337.991 kb on + strand, within cysK at 337.991 kb on + strand, within cysK at 337.991 kb on + strand, within cysK at 337.992 kb on - strand, within cysK at 337.992 kb on - strand, within cysK at 337.992 kb on - strand, within cysK at 337.992 kb on - strand, within cysK at 337.992 kb on - strand, within cysK at 337.992 kb on - strand, within cysK at 337.992 kb on - strand, within cysK at 337.992 kb on - strand, within cysK at 338.267 kb on + strand, within cysK at 338.285 kb on + strand, within cysK at 338.285 kb on + strand, within cysK at 338.285 kb on + strand, within cysK at 338.285 kb on + strand, within cysK at 338.285 kb on + strand, within cysK at 338.285 kb on + strand, within cysK at 338.285 kb on + strand, within cysK at 338.285 kb on + strand, within cysK at 338.285 kb on + strand, within cysK at 338.286 kb on - strand, within cysK at 338.286 kb on - strand, within cysK at 338.286 kb on - strand, within cysK at 338.286 kb on - strand, within cysK at 338.286 kb on - strand, within cysK at 338.286 kb on - strand, within cysK at 338.286 kb on - strand, within cysK at 338.286 kb on - strand, within cysK at 338.824 kb on + strand at 338.824 kb on + strand at 338.825 kb on - strand at 338.825 kb on - strand at 338.854 kb on + strand at 338.854 kb on + strand at 338.855 kb on - strand at 339.013 kb on + strand, within OKGIIK_01505 at 339.014 kb on - strand, within OKGIIK_01505 at 339.014 kb on - strand, within OKGIIK_01505 at 339.106 kb on + strand, within OKGIIK_01505 at 339.106 kb on + strand, within OKGIIK_01505 at 339.107 kb on - strand, within OKGIIK_01505 at 339.107 kb on - strand, within OKGIIK_01505 at 339.107 kb on - strand, within OKGIIK_01505 at 339.148 kb on + strand, within OKGIIK_01505 at 339.149 kb on - strand, within OKGIIK_01505 at 339.149 kb on - strand, within OKGIIK_01505 at 339.149 kb on - strand, within OKGIIK_01505 at 339.149 kb on - strand, within OKGIIK_01505 at 339.149 kb on - strand, within OKGIIK_01505 at 339.149 kb on - strand, within OKGIIK_01505 at 339.168 kb on + strand, within OKGIIK_01505 at 339.168 kb on + strand, within OKGIIK_01505 at 339.169 kb on - strand, within OKGIIK_01505 at 339.353 kb on - strand, within OKGIIK_01505 at 339.353 kb on - strand, within OKGIIK_01505 at 339.380 kb on + strand at 339.507 kb on - strand at 339.512 kb on + strand at 339.512 kb on + strand at 339.512 kb on + strand at 339.512 kb on + strand at 339.512 kb on + strand at 339.512 kb on + strand at 339.512 kb on + strand at 339.512 kb on + strand at 339.512 kb on + strand at 339.512 kb on + strand at 339.512 kb on + strand at 339.512 kb on + strand at 339.512 kb on + strand at 339.512 kb on + strand at 339.512 kb on + strand at 339.512 kb on + strand at 339.513 kb on - strand at 339.513 kb on - strand at 339.513 kb on - strand at 339.513 kb on - strand at 339.513 kb on - strand at 339.513 kb on - strand at 339.513 kb on - strand at 339.513 kb on - strand at 339.514 kb on + strand at 339.514 kb on + strand at 339.514 kb on + strand at 339.514 kb on + strand at 339.514 kb on + strand at 339.514 kb on + strand at 339.515 kb on - strand at 339.515 kb on - strand at 339.515 kb on - strand at 339.515 kb on - strand at 339.515 kb on - strand at 339.515 kb on - strand at 339.515 kb on - strand at 339.515 kb on - strand at 339.515 kb on - strand at 339.515 kb on - strand at 339.522 kb on + strand at 339.522 kb on + strand at 339.523 kb on - strand at 339.538 kb on + strand at 339.538 kb on + strand at 339.538 kb on + strand at 339.539 kb on - strand at 339.539 kb on - strand at 339.539 kb on - strand at 339.549 kb on + strand at 339.549 kb on + strand at 339.549 kb on + strand at 339.549 kb on + strand at 339.549 kb on + strand at 339.549 kb on + strand at 339.549 kb on + strand at 339.549 kb on + strand at 339.550 kb on - strand at 339.550 kb on - strand at 339.550 kb on - strand at 339.550 kb on - strand at 339.550 kb on - strand at 339.550 kb on - strand at 339.550 kb on - strand at 339.550 kb on - strand at 339.550 kb on - strand at 339.551 kb on + strand at 339.552 kb on - strand at 339.552 kb on - strand at 339.552 kb on - strand at 339.552 kb on - strand at 339.803 kb on + strand at 339.804 kb on - strand at 339.804 kb on - strand at 339.812 kb on + strand at 339.905 kb on + strand, within mET2 at 339.905 kb on + strand, within mET2 at 339.905 kb on + strand, within mET2 at 339.906 kb on - strand, within mET2 at 339.906 kb on - strand, within mET2 at 339.906 kb on - strand, within mET2 at 339.906 kb on - strand, within mET2 at 339.906 kb on - strand, within mET2 at 339.906 kb on - strand, within mET2 at 339.906 kb on - strand, within mET2 at 339.909 kb on + strand, within mET2 at 339.909 kb on + strand, within mET2 at 339.910 kb on - strand, within mET2 at 339.910 kb on - strand, within mET2 at 339.910 kb on - strand, within mET2 at 339.910 kb on - strand, within mET2 at 339.942 kb on + strand, within mET2 at 339.942 kb on + strand, within mET2 at 339.942 kb on + strand, within mET2 at 339.942 kb on + strand, within mET2 at 339.942 kb on + strand, within mET2 at 339.942 kb on + strand, within mET2 at 339.942 kb on + strand, within mET2 at 339.942 kb on + strand, within mET2 at 339.942 kb on + strand, within mET2 at 339.942 kb on + strand, within mET2 at 339.943 kb on - strand, within mET2 at 339.943 kb on - strand, within mET2 at 339.943 kb on - strand, within mET2 at 339.943 kb on - strand, within mET2 at 339.943 kb on - strand, within mET2 at 339.943 kb on - strand, within mET2 at 339.943 kb on - strand, within mET2 at 339.943 kb on - strand, within mET2 at 339.943 kb on - strand, within mET2 at 339.943 kb on - strand, within mET2 at 339.943 kb on - strand, within mET2 at 339.943 kb on - strand, within mET2 at 339.943 kb on - strand, within mET2 at 340.233 kb on + strand, within mET2 at 340.233 kb on + strand, within mET2 at 340.234 kb on - strand, within mET2 at 340.234 kb on - strand, within mET2
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES pH 4 remove 337,991 + cysK OKGIIK_01500 0.35 -1.9 337,991 + cysK OKGIIK_01500 0.35 -3.7 337,991 + cysK OKGIIK_01500 0.35 +0.5 337,991 + cysK OKGIIK_01500 0.35 -0.0 337,991 + cysK OKGIIK_01500 0.35 -1.9 337,991 + cysK OKGIIK_01500 0.35 -2.3 337,991 + cysK OKGIIK_01500 0.35 +1.1 337,991 + cysK OKGIIK_01500 0.35 +0.8 337,991 + cysK OKGIIK_01500 0.35 -1.8 337,991 + cysK OKGIIK_01500 0.35 -2.2 337,991 + cysK OKGIIK_01500 0.35 -1.7 337,991 + cysK OKGIIK_01500 0.35 +0.0 337,992 - cysK OKGIIK_01500 0.36 +1.5 337,992 - cysK OKGIIK_01500 0.36 +1.0 337,992 - cysK OKGIIK_01500 0.36 +0.5 337,992 - cysK OKGIIK_01500 0.36 -0.5 337,992 - cysK OKGIIK_01500 0.36 -2.7 337,992 - cysK OKGIIK_01500 0.36 -0.4 337,992 - cysK OKGIIK_01500 0.36 -0.0 337,992 - cysK OKGIIK_01500 0.36 -1.6 338,267 + cysK OKGIIK_01500 0.63 -1.0 338,285 + cysK OKGIIK_01500 0.64 +0.2 338,285 + cysK OKGIIK_01500 0.64 -0.8 338,285 + cysK OKGIIK_01500 0.64 -0.8 338,285 + cysK OKGIIK_01500 0.64 +0.2 338,285 + cysK OKGIIK_01500 0.64 -1.3 338,285 + cysK OKGIIK_01500 0.64 -0.3 338,285 + cysK OKGIIK_01500 0.64 -0.8 338,285 + cysK OKGIIK_01500 0.64 -0.6 338,285 + cysK OKGIIK_01500 0.64 -1.3 338,286 - cysK OKGIIK_01500 0.65 -0.8 338,286 - cysK OKGIIK_01500 0.65 -2.0 338,286 - cysK OKGIIK_01500 0.65 -1.5 338,286 - cysK OKGIIK_01500 0.65 -1.3 338,286 - cysK OKGIIK_01500 0.65 -0.3 338,286 - cysK OKGIIK_01500 0.65 +1.5 338,286 - cysK OKGIIK_01500 0.65 -0.3 338,286 - cysK OKGIIK_01500 0.65 -0.5 338,824 + -0.0 338,824 + -1.3 338,825 - -0.8 338,825 - -0.5 338,854 + +1.9 338,854 + -1.3 338,855 - -0.2 339,013 + OKGIIK_01505 0.35 -1.5 339,014 - OKGIIK_01505 0.35 -1.5 339,014 - OKGIIK_01505 0.35 -0.7 339,106 + OKGIIK_01505 0.49 +0.7 339,106 + OKGIIK_01505 0.49 +1.5 339,107 - OKGIIK_01505 0.49 -1.5 339,107 - OKGIIK_01505 0.49 -1.4 339,107 - OKGIIK_01505 0.49 -0.5 339,148 + OKGIIK_01505 0.56 -0.8 339,149 - OKGIIK_01505 0.56 +1.1 339,149 - OKGIIK_01505 0.56 -1.0 339,149 - OKGIIK_01505 0.56 -1.6 339,149 - OKGIIK_01505 0.56 -2.4 339,149 - OKGIIK_01505 0.56 -1.5 339,149 - OKGIIK_01505 0.56 -2.5 339,168 + OKGIIK_01505 0.59 -2.7 339,168 + OKGIIK_01505 0.59 -0.5 339,169 - OKGIIK_01505 0.59 -0.2 339,353 - OKGIIK_01505 0.88 +0.8 339,353 - OKGIIK_01505 0.88 -1.3 339,380 + -1.0 339,507 - -0.0 339,512 + -0.5 339,512 + -2.3 339,512 + -0.8 339,512 + -0.8 339,512 + -0.0 339,512 + -1.4 339,512 + -2.6 339,512 + -0.6 339,512 + -1.8 339,512 + +0.3 339,512 + -2.4 339,512 + -1.8 339,512 + -1.3 339,512 + -2.0 339,512 + -0.1 339,512 + -0.7 339,513 - -0.5 339,513 - -0.5 339,513 - -0.4 339,513 - -1.0 339,513 - -2.0 339,513 - -0.9 339,513 - -0.7 339,513 - -1.8 339,514 + +0.2 339,514 + -3.0 339,514 + -0.6 339,514 + +0.5 339,514 + -1.6 339,514 + -1.6 339,515 - -1.6 339,515 - -0.2 339,515 - -0.3 339,515 - -2.0 339,515 - +0.5 339,515 - -0.0 339,515 - -0.8 339,515 - -0.9 339,515 - -0.0 339,515 - -1.0 339,522 + -1.0 339,522 + -1.0 339,523 - -0.3 339,538 + -0.7 339,538 + -1.3 339,538 + -0.8 339,539 - +1.0 339,539 - -0.5 339,539 - -1.0 339,549 + -1.3 339,549 + -0.5 339,549 + -1.5 339,549 + -0.5 339,549 + -1.0 339,549 + -1.3 339,549 + -1.6 339,549 + -1.0 339,550 - +0.5 339,550 - +1.5 339,550 - -2.0 339,550 - -1.6 339,550 - +0.5 339,550 - -0.2 339,550 - -0.5 339,550 - -1.3 339,550 - +0.5 339,551 + -2.3 339,552 - -1.2 339,552 - -2.2 339,552 - -2.4 339,552 - -2.2 339,803 + -1.0 339,804 - -1.5 339,804 - -0.8 339,812 + -1.3 339,905 + mET2 OKGIIK_01510 0.12 -1.7 339,905 + mET2 OKGIIK_01510 0.12 -0.5 339,905 + mET2 OKGIIK_01510 0.12 +1.1 339,906 - mET2 OKGIIK_01510 0.12 -2.6 339,906 - mET2 OKGIIK_01510 0.12 -1.6 339,906 - mET2 OKGIIK_01510 0.12 -0.0 339,906 - mET2 OKGIIK_01510 0.12 -1.9 339,906 - mET2 OKGIIK_01510 0.12 +0.4 339,906 - mET2 OKGIIK_01510 0.12 +1.5 339,906 - mET2 OKGIIK_01510 0.12 +0.2 339,909 + mET2 OKGIIK_01510 0.13 -1.2 339,909 + mET2 OKGIIK_01510 0.13 -0.3 339,910 - mET2 OKGIIK_01510 0.13 -1.3 339,910 - mET2 OKGIIK_01510 0.13 -2.4 339,910 - mET2 OKGIIK_01510 0.13 -0.0 339,910 - mET2 OKGIIK_01510 0.13 -0.5 339,942 + mET2 OKGIIK_01510 0.16 -0.5 339,942 + mET2 OKGIIK_01510 0.16 -0.6 339,942 + mET2 OKGIIK_01510 0.16 +0.2 339,942 + mET2 OKGIIK_01510 0.16 -0.8 339,942 + mET2 OKGIIK_01510 0.16 -0.0 339,942 + mET2 OKGIIK_01510 0.16 -2.0 339,942 + mET2 OKGIIK_01510 0.16 -2.4 339,942 + mET2 OKGIIK_01510 0.16 +1.0 339,942 + mET2 OKGIIK_01510 0.16 +0.3 339,942 + mET2 OKGIIK_01510 0.16 +0.5 339,943 - mET2 OKGIIK_01510 0.16 +1.1 339,943 - mET2 OKGIIK_01510 0.16 -2.0 339,943 - mET2 OKGIIK_01510 0.16 -1.0 339,943 - mET2 OKGIIK_01510 0.16 -1.3 339,943 - mET2 OKGIIK_01510 0.16 -1.3 339,943 - mET2 OKGIIK_01510 0.16 -1.0 339,943 - mET2 OKGIIK_01510 0.16 -1.7 339,943 - mET2 OKGIIK_01510 0.16 -1.1 339,943 - mET2 OKGIIK_01510 0.16 +0.7 339,943 - mET2 OKGIIK_01510 0.16 -1.5 339,943 - mET2 OKGIIK_01510 0.16 -1.5 339,943 - mET2 OKGIIK_01510 0.16 -3.0 339,943 - mET2 OKGIIK_01510 0.16 -1.3 340,233 + mET2 OKGIIK_01510 0.44 -0.0 340,233 + mET2 OKGIIK_01510 0.44 -0.8 340,234 - mET2 OKGIIK_01510 0.44 -0.0 340,234 - mET2 OKGIIK_01510 0.44 -1.0
Or see this region's nucleotide sequence