Experiment: R2A_PIPES pH 4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cpsB and rfbD are separated by 16 nucleotides rfbD and rfbC are separated by 143 nucleotides rfbC and rfbA overlap by 4 nucleotides
OKGIIK_01155: cpsB - mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase, at 248,823 to 250,235
cpsB
OKGIIK_01160: rfbD - dTDP-4-dehydrorhamnose reductase, at 250,252 to 251,187
rfbD
OKGIIK_01165: rfbC - dTDP-4-dehydrorhamnose 3,5-epimerase, at 251,331 to 251,888
rfbC
OKGIIK_01170: rfbA - glucose-1-phosphate thymidylyltransferase RfbA, at 251,885 to 252,769
rfbA
Position (kb) 250
251
252 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 249.487 kb on + strand, within cpsB at 249.568 kb on + strand, within cpsB at 249.568 kb on + strand, within cpsB at 249.568 kb on + strand, within cpsB at 249.569 kb on - strand, within cpsB at 249.626 kb on - strand, within cpsB at 249.626 kb on - strand, within cpsB at 249.667 kb on + strand, within cpsB at 249.685 kb on + strand, within cpsB at 249.685 kb on + strand, within cpsB at 249.686 kb on - strand, within cpsB at 249.686 kb on - strand, within cpsB at 249.686 kb on - strand, within cpsB at 249.772 kb on + strand, within cpsB at 249.772 kb on + strand, within cpsB at 249.772 kb on + strand, within cpsB at 249.772 kb on + strand, within cpsB at 249.772 kb on + strand, within cpsB at 249.772 kb on + strand, within cpsB at 249.772 kb on + strand, within cpsB at 249.772 kb on + strand, within cpsB at 249.772 kb on + strand, within cpsB at 249.773 kb on - strand, within cpsB at 249.773 kb on - strand, within cpsB at 249.773 kb on - strand, within cpsB at 249.773 kb on - strand, within cpsB at 249.773 kb on - strand, within cpsB at 249.773 kb on - strand, within cpsB at 249.906 kb on + strand, within cpsB at 249.907 kb on - strand, within cpsB at 249.907 kb on - strand, within cpsB at 249.907 kb on - strand, within cpsB at 249.907 kb on - strand, within cpsB at 250.016 kb on + strand, within cpsB at 250.016 kb on + strand, within cpsB at 250.017 kb on - strand, within cpsB at 250.199 kb on + strand at 250.268 kb on - strand at 250.348 kb on - strand, within rfbD at 250.700 kb on + strand, within rfbD at 250.701 kb on - strand, within rfbD at 250.781 kb on + strand, within rfbD at 250.817 kb on + strand, within rfbD at 250.817 kb on + strand, within rfbD at 250.817 kb on + strand, within rfbD at 250.847 kb on + strand, within rfbD at 250.847 kb on + strand, within rfbD at 250.847 kb on + strand, within rfbD at 250.847 kb on + strand, within rfbD at 250.848 kb on - strand, within rfbD at 250.848 kb on - strand, within rfbD at 250.848 kb on - strand, within rfbD at 250.848 kb on - strand, within rfbD at 250.848 kb on - strand, within rfbD at 250.859 kb on + strand, within rfbD at 250.859 kb on + strand, within rfbD at 250.859 kb on + strand, within rfbD at 250.859 kb on + strand, within rfbD at 250.859 kb on + strand, within rfbD at 250.860 kb on - strand, within rfbD at 250.860 kb on - strand, within rfbD at 250.860 kb on - strand, within rfbD at 250.860 kb on - strand, within rfbD at 250.860 kb on - strand, within rfbD at 250.973 kb on + strand, within rfbD at 250.973 kb on + strand, within rfbD at 250.974 kb on - strand, within rfbD at 250.974 kb on - strand, within rfbD at 250.974 kb on - strand, within rfbD at 251.176 kb on + strand at 251.176 kb on + strand at 251.176 kb on + strand at 251.176 kb on + strand at 251.176 kb on + strand at 251.176 kb on + strand at 251.177 kb on - strand at 251.177 kb on - strand at 251.177 kb on - strand at 251.177 kb on - strand at 251.177 kb on - strand at 251.177 kb on - strand at 251.177 kb on - strand at 251.177 kb on - strand at 251.177 kb on - strand at 251.203 kb on + strand at 251.203 kb on + strand at 251.203 kb on + strand at 251.203 kb on + strand at 251.203 kb on + strand at 251.203 kb on + strand at 251.332 kb on + strand at 251.333 kb on - strand at 251.333 kb on - strand at 251.333 kb on - strand at 251.341 kb on + strand at 251.341 kb on + strand at 251.341 kb on + strand at 251.341 kb on + strand at 251.342 kb on - strand at 251.342 kb on - strand at 251.482 kb on + strand, within rfbC at 251.482 kb on + strand, within rfbC at 251.483 kb on - strand, within rfbC at 251.483 kb on - strand, within rfbC at 251.483 kb on - strand, within rfbC at 251.483 kb on - strand, within rfbC at 251.483 kb on - strand, within rfbC at 251.483 kb on - strand, within rfbC at 251.500 kb on + strand, within rfbC at 251.500 kb on + strand, within rfbC at 251.500 kb on + strand, within rfbC at 251.500 kb on + strand, within rfbC at 251.500 kb on + strand, within rfbC at 251.500 kb on + strand, within rfbC at 251.501 kb on - strand, within rfbC at 251.501 kb on - strand, within rfbC at 251.501 kb on - strand, within rfbC at 251.633 kb on + strand, within rfbC at 251.650 kb on + strand, within rfbC at 251.651 kb on - strand, within rfbC at 251.651 kb on - strand, within rfbC at 251.651 kb on - strand, within rfbC at 251.651 kb on - strand, within rfbC at 251.705 kb on - strand, within rfbC at 251.705 kb on - strand, within rfbC at 251.754 kb on + strand, within rfbC at 251.754 kb on + strand, within rfbC at 251.755 kb on - strand, within rfbC at 251.800 kb on + strand, within rfbC at 251.800 kb on + strand, within rfbC at 251.800 kb on + strand, within rfbC at 251.800 kb on + strand, within rfbC at 251.801 kb on - strand, within rfbC at 251.801 kb on - strand, within rfbC at 251.801 kb on - strand, within rfbC at 251.801 kb on - strand, within rfbC at 251.809 kb on + strand, within rfbC at 251.809 kb on + strand, within rfbC at 251.809 kb on + strand, within rfbC at 251.810 kb on - strand, within rfbC at 251.810 kb on - strand, within rfbC at 251.922 kb on + strand at 251.922 kb on + strand at 251.923 kb on - strand at 251.923 kb on - strand at 251.923 kb on - strand at 251.998 kb on - strand, within rfbA at 252.057 kb on + strand, within rfbA at 252.057 kb on + strand, within rfbA at 252.057 kb on + strand, within rfbA at 252.057 kb on + strand, within rfbA at 252.057 kb on + strand, within rfbA at 252.057 kb on + strand, within rfbA at 252.057 kb on + strand, within rfbA at 252.057 kb on + strand, within rfbA at 252.058 kb on - strand, within rfbA at 252.058 kb on - strand, within rfbA at 252.058 kb on - strand, within rfbA at 252.058 kb on - strand, within rfbA at 252.058 kb on - strand, within rfbA at 252.058 kb on - strand, within rfbA at 252.058 kb on - strand, within rfbA at 252.058 kb on - strand, within rfbA at 252.058 kb on - strand, within rfbA at 252.153 kb on + strand, within rfbA at 252.153 kb on + strand, within rfbA at 252.153 kb on + strand, within rfbA at 252.153 kb on + strand, within rfbA at 252.153 kb on + strand, within rfbA at 252.154 kb on - strand, within rfbA at 252.154 kb on - strand, within rfbA at 252.154 kb on - strand, within rfbA
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES pH 4 remove 249,487 + cpsB OKGIIK_01155 0.47 -0.8 249,568 + cpsB OKGIIK_01155 0.53 -0.8 249,568 + cpsB OKGIIK_01155 0.53 -2.7 249,568 + cpsB OKGIIK_01155 0.53 -0.8 249,569 - cpsB OKGIIK_01155 0.53 -0.5 249,626 - cpsB OKGIIK_01155 0.57 +0.4 249,626 - cpsB OKGIIK_01155 0.57 +0.2 249,667 + cpsB OKGIIK_01155 0.60 -1.1 249,685 + cpsB OKGIIK_01155 0.61 -0.8 249,685 + cpsB OKGIIK_01155 0.61 -1.3 249,686 - cpsB OKGIIK_01155 0.61 -1.5 249,686 - cpsB OKGIIK_01155 0.61 -0.8 249,686 - cpsB OKGIIK_01155 0.61 -0.8 249,772 + cpsB OKGIIK_01155 0.67 -1.5 249,772 + cpsB OKGIIK_01155 0.67 -0.5 249,772 + cpsB OKGIIK_01155 0.67 -0.5 249,772 + cpsB OKGIIK_01155 0.67 -0.5 249,772 + cpsB OKGIIK_01155 0.67 +1.1 249,772 + cpsB OKGIIK_01155 0.67 -2.1 249,772 + cpsB OKGIIK_01155 0.67 -1.5 249,772 + cpsB OKGIIK_01155 0.67 +0.5 249,772 + cpsB OKGIIK_01155 0.67 -0.5 249,773 - cpsB OKGIIK_01155 0.67 -0.3 249,773 - cpsB OKGIIK_01155 0.67 -1.1 249,773 - cpsB OKGIIK_01155 0.67 -0.5 249,773 - cpsB OKGIIK_01155 0.67 -1.1 249,773 - cpsB OKGIIK_01155 0.67 -0.5 249,773 - cpsB OKGIIK_01155 0.67 -1.7 249,906 + cpsB OKGIIK_01155 0.77 -1.5 249,907 - cpsB OKGIIK_01155 0.77 -1.1 249,907 - cpsB OKGIIK_01155 0.77 -0.8 249,907 - cpsB OKGIIK_01155 0.77 -1.1 249,907 - cpsB OKGIIK_01155 0.77 -1.8 250,016 + cpsB OKGIIK_01155 0.84 -0.5 250,016 + cpsB OKGIIK_01155 0.84 -0.5 250,017 - cpsB OKGIIK_01155 0.85 -1.1 250,199 + +1.5 250,268 - -0.9 250,348 - rfbD OKGIIK_01160 0.10 -1.5 250,700 + rfbD OKGIIK_01160 0.48 -0.5 250,701 - rfbD OKGIIK_01160 0.48 -1.8 250,781 + rfbD OKGIIK_01160 0.57 -0.5 250,817 + rfbD OKGIIK_01160 0.60 -2.2 250,817 + rfbD OKGIIK_01160 0.60 -0.5 250,817 + rfbD OKGIIK_01160 0.60 -0.7 250,847 + rfbD OKGIIK_01160 0.64 -0.1 250,847 + rfbD OKGIIK_01160 0.64 -0.8 250,847 + rfbD OKGIIK_01160 0.64 -0.5 250,847 + rfbD OKGIIK_01160 0.64 -0.5 250,848 - rfbD OKGIIK_01160 0.64 -0.8 250,848 - rfbD OKGIIK_01160 0.64 +1.5 250,848 - rfbD OKGIIK_01160 0.64 -0.7 250,848 - rfbD OKGIIK_01160 0.64 -1.3 250,848 - rfbD OKGIIK_01160 0.64 -1.1 250,859 + rfbD OKGIIK_01160 0.65 -2.4 250,859 + rfbD OKGIIK_01160 0.65 -1.3 250,859 + rfbD OKGIIK_01160 0.65 -2.2 250,859 + rfbD OKGIIK_01160 0.65 -0.5 250,859 + rfbD OKGIIK_01160 0.65 -0.2 250,860 - rfbD OKGIIK_01160 0.65 -0.5 250,860 - rfbD OKGIIK_01160 0.65 -1.8 250,860 - rfbD OKGIIK_01160 0.65 -0.5 250,860 - rfbD OKGIIK_01160 0.65 -1.7 250,860 - rfbD OKGIIK_01160 0.65 -1.3 250,973 + rfbD OKGIIK_01160 0.77 -0.8 250,973 + rfbD OKGIIK_01160 0.77 -0.5 250,974 - rfbD OKGIIK_01160 0.77 -0.5 250,974 - rfbD OKGIIK_01160 0.77 +1.0 250,974 - rfbD OKGIIK_01160 0.77 -0.5 251,176 + -2.5 251,176 + -1.2 251,176 + -0.8 251,176 + +0.4 251,176 + -0.8 251,176 + -1.3 251,177 - -1.1 251,177 - +0.6 251,177 - +0.5 251,177 - -1.8 251,177 - -1.1 251,177 - -0.7 251,177 - -0.5 251,177 - +1.3 251,177 - +1.2 251,203 + -0.5 251,203 + -0.8 251,203 + -1.3 251,203 + +0.1 251,203 + -1.3 251,203 + -1.7 251,332 + -1.1 251,333 - -1.1 251,333 - -0.1 251,333 - -1.8 251,341 + -0.3 251,341 + -0.5 251,341 + -0.0 251,341 + +2.5 251,342 - -1.3 251,342 - -1.0 251,482 + rfbC OKGIIK_01165 0.27 -1.1 251,482 + rfbC OKGIIK_01165 0.27 -1.1 251,483 - rfbC OKGIIK_01165 0.27 -0.5 251,483 - rfbC OKGIIK_01165 0.27 +0.0 251,483 - rfbC OKGIIK_01165 0.27 +0.5 251,483 - rfbC OKGIIK_01165 0.27 +1.1 251,483 - rfbC OKGIIK_01165 0.27 -0.5 251,483 - rfbC OKGIIK_01165 0.27 -1.1 251,500 + rfbC OKGIIK_01165 0.30 -0.5 251,500 + rfbC OKGIIK_01165 0.30 -0.6 251,500 + rfbC OKGIIK_01165 0.30 -0.8 251,500 + rfbC OKGIIK_01165 0.30 -0.7 251,500 + rfbC OKGIIK_01165 0.30 -0.8 251,500 + rfbC OKGIIK_01165 0.30 -1.5 251,501 - rfbC OKGIIK_01165 0.30 -0.1 251,501 - rfbC OKGIIK_01165 0.30 +0.5 251,501 - rfbC OKGIIK_01165 0.30 -0.6 251,633 + rfbC OKGIIK_01165 0.54 -1.0 251,650 + rfbC OKGIIK_01165 0.57 -1.3 251,651 - rfbC OKGIIK_01165 0.57 -1.5 251,651 - rfbC OKGIIK_01165 0.57 -0.5 251,651 - rfbC OKGIIK_01165 0.57 -0.8 251,651 - rfbC OKGIIK_01165 0.57 +0.2 251,705 - rfbC OKGIIK_01165 0.67 -0.8 251,705 - rfbC OKGIIK_01165 0.67 -0.5 251,754 + rfbC OKGIIK_01165 0.76 -0.5 251,754 + rfbC OKGIIK_01165 0.76 -2.4 251,755 - rfbC OKGIIK_01165 0.76 +0.5 251,800 + rfbC OKGIIK_01165 0.84 -0.6 251,800 + rfbC OKGIIK_01165 0.84 -0.8 251,800 + rfbC OKGIIK_01165 0.84 -1.6 251,800 + rfbC OKGIIK_01165 0.84 -1.3 251,801 - rfbC OKGIIK_01165 0.84 -0.5 251,801 - rfbC OKGIIK_01165 0.84 -0.5 251,801 - rfbC OKGIIK_01165 0.84 -0.8 251,801 - rfbC OKGIIK_01165 0.84 -2.9 251,809 + rfbC OKGIIK_01165 0.86 -0.5 251,809 + rfbC OKGIIK_01165 0.86 -1.3 251,809 + rfbC OKGIIK_01165 0.86 -0.5 251,810 - rfbC OKGIIK_01165 0.86 -0.3 251,810 - rfbC OKGIIK_01165 0.86 -1.8 251,922 + -1.7 251,922 + -1.3 251,923 - -1.3 251,923 - -2.7 251,923 - -2.0 251,998 - rfbA OKGIIK_01170 0.13 -1.1 252,057 + rfbA OKGIIK_01170 0.19 -1.1 252,057 + rfbA OKGIIK_01170 0.19 -1.3 252,057 + rfbA OKGIIK_01170 0.19 -1.1 252,057 + rfbA OKGIIK_01170 0.19 -0.5 252,057 + rfbA OKGIIK_01170 0.19 -0.5 252,057 + rfbA OKGIIK_01170 0.19 -2.7 252,057 + rfbA OKGIIK_01170 0.19 -0.8 252,057 + rfbA OKGIIK_01170 0.19 -0.1 252,058 - rfbA OKGIIK_01170 0.20 -1.8 252,058 - rfbA OKGIIK_01170 0.20 -1.1 252,058 - rfbA OKGIIK_01170 0.20 -2.1 252,058 - rfbA OKGIIK_01170 0.20 -0.5 252,058 - rfbA OKGIIK_01170 0.20 -1.7 252,058 - rfbA OKGIIK_01170 0.20 -0.8 252,058 - rfbA OKGIIK_01170 0.20 -0.5 252,058 - rfbA OKGIIK_01170 0.20 -0.8 252,058 - rfbA OKGIIK_01170 0.20 -1.1 252,153 + rfbA OKGIIK_01170 0.30 -0.5 252,153 + rfbA OKGIIK_01170 0.30 -1.7 252,153 + rfbA OKGIIK_01170 0.30 -1.0 252,153 + rfbA OKGIIK_01170 0.30 -0.5 252,153 + rfbA OKGIIK_01170 0.30 -2.1 252,154 - rfbA OKGIIK_01170 0.30 -1.7 252,154 - rfbA OKGIIK_01170 0.30 -1.0 252,154 - rfbA OKGIIK_01170 0.30 -2.7
Or see this region's nucleotide sequence