Experiment: R2A_PIPES pH 4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OKGIIK_01130 and ydcH are separated by 363 nucleotides ydcH and aF2118 are separated by 160 nucleotides aF2118 and glmS are separated by 133 nucleotides
OKGIIK_01130: OKGIIK_01130 - DUF4398 domain-containing protein, at 242,407 to 243,048
_01130
OKGIIK_01135: ydcH - DUF465 domain-containing protein, at 243,412 to 243,639
ydcH
OKGIIK_01140: aF2118 - cystathionine beta-synthase, at 243,800 to 245,170
aF2118
OKGIIK_01145: glmS - glutamine--fructose-6-phosphate transaminase (isomerizing), at 245,304 to 247,133
glmS
Position (kb) 243
244
245
246 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 242.800 kb on + strand, within OKGIIK_01130 at 242.800 kb on + strand, within OKGIIK_01130 at 242.801 kb on - strand, within OKGIIK_01130 at 242.801 kb on - strand, within OKGIIK_01130 at 242.801 kb on - strand, within OKGIIK_01130 at 242.801 kb on - strand, within OKGIIK_01130 at 242.851 kb on + strand, within OKGIIK_01130 at 242.851 kb on + strand, within OKGIIK_01130 at 242.851 kb on + strand, within OKGIIK_01130 at 242.851 kb on + strand, within OKGIIK_01130 at 242.852 kb on - strand, within OKGIIK_01130 at 242.852 kb on - strand, within OKGIIK_01130 at 242.852 kb on - strand, within OKGIIK_01130 at 242.852 kb on - strand, within OKGIIK_01130 at 242.852 kb on - strand, within OKGIIK_01130 at 243.130 kb on + strand at 243.130 kb on + strand at 243.225 kb on + strand at 243.225 kb on + strand at 243.225 kb on + strand at 243.225 kb on + strand at 243.225 kb on + strand at 243.225 kb on + strand at 243.225 kb on + strand at 243.225 kb on + strand at 243.225 kb on + strand at 243.225 kb on + strand at 243.226 kb on - strand at 243.226 kb on - strand at 243.226 kb on - strand at 243.226 kb on - strand at 243.226 kb on - strand at 243.226 kb on - strand at 243.226 kb on - strand at 243.226 kb on - strand at 243.226 kb on - strand at 243.226 kb on - strand at 243.226 kb on - strand at 243.285 kb on + strand at 243.285 kb on + strand at 243.285 kb on + strand at 243.285 kb on + strand at 243.285 kb on + strand at 243.285 kb on + strand at 243.286 kb on - strand at 243.286 kb on - strand at 243.286 kb on - strand at 243.286 kb on - strand at 243.286 kb on - strand at 243.286 kb on - strand at 243.286 kb on - strand at 243.286 kb on - strand at 243.286 kb on - strand at 243.286 kb on - strand at 243.286 kb on - strand at 243.286 kb on - strand at 243.286 kb on - strand at 243.286 kb on - strand at 243.481 kb on + strand, within ydcH at 243.481 kb on + strand, within ydcH at 243.481 kb on + strand, within ydcH at 243.481 kb on + strand, within ydcH at 243.481 kb on + strand, within ydcH at 243.481 kb on + strand, within ydcH at 243.481 kb on + strand, within ydcH at 243.482 kb on - strand, within ydcH at 243.482 kb on - strand, within ydcH at 243.482 kb on - strand, within ydcH at 243.685 kb on - strand at 243.685 kb on - strand at 243.740 kb on + strand at 243.740 kb on + strand at 243.741 kb on - strand at 243.838 kb on + strand at 243.838 kb on + strand at 244.175 kb on + strand, within aF2118 at 244.175 kb on + strand, within aF2118 at 244.175 kb on + strand, within aF2118 at 244.175 kb on + strand, within aF2118 at 244.176 kb on - strand, within aF2118 at 244.176 kb on - strand, within aF2118 at 244.176 kb on - strand, within aF2118 at 244.176 kb on - strand, within aF2118 at 244.176 kb on - strand, within aF2118 at 244.178 kb on + strand, within aF2118 at 244.178 kb on + strand, within aF2118 at 244.178 kb on + strand, within aF2118 at 244.178 kb on + strand, within aF2118 at 244.179 kb on - strand, within aF2118 at 244.179 kb on - strand, within aF2118 at 244.179 kb on - strand, within aF2118 at 244.179 kb on - strand, within aF2118 at 244.179 kb on - strand, within aF2118 at 244.223 kb on + strand, within aF2118 at 244.223 kb on + strand, within aF2118 at 244.224 kb on - strand, within aF2118 at 244.224 kb on - strand, within aF2118 at 244.224 kb on - strand, within aF2118 at 244.224 kb on - strand, within aF2118 at 244.224 kb on - strand, within aF2118 at 244.439 kb on + strand, within aF2118 at 244.439 kb on + strand, within aF2118 at 244.439 kb on + strand, within aF2118 at 244.439 kb on + strand, within aF2118 at 244.439 kb on + strand, within aF2118 at 244.439 kb on + strand, within aF2118 at 244.439 kb on + strand, within aF2118 at 244.439 kb on + strand, within aF2118 at 244.439 kb on + strand, within aF2118 at 244.439 kb on + strand, within aF2118 at 244.439 kb on + strand, within aF2118 at 244.439 kb on + strand, within aF2118 at 244.440 kb on - strand, within aF2118 at 244.440 kb on - strand, within aF2118 at 244.440 kb on - strand, within aF2118 at 244.440 kb on - strand, within aF2118 at 244.440 kb on - strand, within aF2118 at 244.440 kb on - strand, within aF2118 at 244.440 kb on - strand, within aF2118 at 244.440 kb on - strand, within aF2118 at 244.440 kb on - strand, within aF2118 at 244.440 kb on - strand, within aF2118 at 244.440 kb on - strand, within aF2118 at 244.440 kb on - strand, within aF2118 at 244.440 kb on - strand, within aF2118 at 244.440 kb on - strand, within aF2118 at 244.547 kb on + strand, within aF2118 at 244.547 kb on + strand, within aF2118 at 244.547 kb on + strand, within aF2118 at 244.547 kb on + strand, within aF2118 at 244.547 kb on + strand, within aF2118 at 244.548 kb on - strand, within aF2118 at 244.548 kb on - strand, within aF2118 at 244.548 kb on - strand, within aF2118 at 244.548 kb on - strand, within aF2118 at 244.548 kb on - strand, within aF2118 at 244.548 kb on - strand, within aF2118 at 244.548 kb on - strand, within aF2118 at 244.655 kb on + strand, within aF2118 at 244.656 kb on - strand, within aF2118 at 244.656 kb on - strand, within aF2118 at 244.718 kb on + strand, within aF2118 at 244.718 kb on + strand, within aF2118 at 244.718 kb on + strand, within aF2118 at 244.718 kb on + strand, within aF2118 at 244.718 kb on + strand, within aF2118 at 244.718 kb on + strand, within aF2118 at 244.718 kb on + strand, within aF2118 at 244.718 kb on + strand, within aF2118 at 244.718 kb on + strand, within aF2118 at 244.718 kb on + strand, within aF2118 at 244.718 kb on + strand, within aF2118 at 244.718 kb on + strand, within aF2118 at 244.718 kb on + strand, within aF2118 at 244.719 kb on - strand, within aF2118 at 244.719 kb on - strand, within aF2118 at 244.719 kb on - strand, within aF2118 at 244.719 kb on - strand, within aF2118 at 244.719 kb on - strand, within aF2118 at 244.719 kb on - strand, within aF2118 at 244.719 kb on - strand, within aF2118 at 244.719 kb on - strand, within aF2118 at 244.719 kb on - strand, within aF2118 at 244.719 kb on - strand, within aF2118 at 244.719 kb on - strand, within aF2118 at 244.719 kb on - strand, within aF2118 at 244.733 kb on + strand, within aF2118 at 244.733 kb on + strand, within aF2118 at 244.733 kb on + strand, within aF2118 at 244.733 kb on + strand, within aF2118 at 244.733 kb on + strand, within aF2118 at 244.733 kb on + strand, within aF2118 at 244.733 kb on + strand, within aF2118 at 244.733 kb on + strand, within aF2118 at 244.734 kb on - strand, within aF2118 at 244.734 kb on - strand, within aF2118 at 244.734 kb on - strand, within aF2118 at 244.734 kb on - strand, within aF2118 at 244.734 kb on - strand, within aF2118 at 244.734 kb on - strand, within aF2118 at 244.742 kb on + strand, within aF2118 at 244.742 kb on + strand, within aF2118 at 244.743 kb on - strand, within aF2118 at 244.743 kb on - strand, within aF2118 at 244.868 kb on + strand, within aF2118 at 244.868 kb on + strand, within aF2118 at 244.868 kb on + strand, within aF2118 at 244.868 kb on + strand, within aF2118 at 244.868 kb on + strand, within aF2118 at 244.868 kb on + strand, within aF2118 at 244.869 kb on - strand, within aF2118 at 244.869 kb on - strand, within aF2118 at 244.869 kb on - strand, within aF2118 at 244.869 kb on - strand, within aF2118 at 244.869 kb on - strand, within aF2118 at 244.869 kb on - strand, within aF2118 at 244.886 kb on + strand, within aF2118 at 244.886 kb on + strand, within aF2118 at 244.886 kb on + strand, within aF2118 at 244.886 kb on + strand, within aF2118 at 244.886 kb on + strand, within aF2118 at 244.886 kb on + strand, within aF2118 at 244.886 kb on + strand, within aF2118 at 244.887 kb on - strand, within aF2118 at 244.887 kb on - strand, within aF2118 at 244.887 kb on - strand, within aF2118 at 244.887 kb on - strand, within aF2118 at 244.887 kb on - strand, within aF2118 at 245.147 kb on + strand at 245.147 kb on + strand at 245.147 kb on + strand at 245.147 kb on + strand at 245.147 kb on + strand at 245.147 kb on + strand at 245.148 kb on - strand at 245.148 kb on - strand at 245.148 kb on - strand at 245.148 kb on - strand at 245.148 kb on - strand at 245.148 kb on - strand at 245.148 kb on - strand at 245.148 kb on - strand at 245.148 kb on - strand at 245.148 kb on - strand at 245.148 kb on - strand at 245.148 kb on - strand at 245.168 kb on + strand at 245.168 kb on + strand at 245.168 kb on + strand at 245.168 kb on + strand at 245.168 kb on + strand at 245.168 kb on + strand at 245.168 kb on + strand at 245.168 kb on + strand at 245.169 kb on - strand at 245.169 kb on - strand at 245.169 kb on - strand at 245.169 kb on - strand at 245.169 kb on - strand at 245.169 kb on - strand at 245.169 kb on - strand at 245.169 kb on - strand at 245.169 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES pH 4 remove 242,800 + OKGIIK_01130 0.61 -2.9 242,800 + OKGIIK_01130 0.61 -1.8 242,801 - OKGIIK_01130 0.61 -0.4 242,801 - OKGIIK_01130 0.61 -0.9 242,801 - OKGIIK_01130 0.61 -0.6 242,801 - OKGIIK_01130 0.61 -0.9 242,851 + OKGIIK_01130 0.69 -0.5 242,851 + OKGIIK_01130 0.69 -1.6 242,851 + OKGIIK_01130 0.69 -0.2 242,851 + OKGIIK_01130 0.69 -1.1 242,852 - OKGIIK_01130 0.69 -1.0 242,852 - OKGIIK_01130 0.69 -0.4 242,852 - OKGIIK_01130 0.69 -1.2 242,852 - OKGIIK_01130 0.69 +0.1 242,852 - OKGIIK_01130 0.69 -0.4 243,130 + -1.8 243,130 + -0.8 243,225 + -1.3 243,225 + -1.9 243,225 + -1.1 243,225 + +1.8 243,225 + -1.1 243,225 + +0.8 243,225 + +0.6 243,225 + +0.4 243,225 + -0.2 243,225 + -0.5 243,226 - +0.4 243,226 - +0.8 243,226 - -1.0 243,226 - +1.5 243,226 - -1.2 243,226 - -0.7 243,226 - -1.3 243,226 - +0.6 243,226 - -0.2 243,226 - -0.7 243,226 - -2.0 243,285 + -0.6 243,285 + -0.9 243,285 + -0.6 243,285 + -0.4 243,285 + +1.9 243,285 + -0.6 243,286 - +0.2 243,286 - +0.2 243,286 - +0.8 243,286 - -1.3 243,286 - -1.5 243,286 - -0.8 243,286 - -1.1 243,286 - -0.1 243,286 - -0.5 243,286 - -0.8 243,286 - +1.2 243,286 - -1.9 243,286 - +0.6 243,286 - +0.1 243,481 + ydcH OKGIIK_01135 0.30 +1.2 243,481 + ydcH OKGIIK_01135 0.30 +1.0 243,481 + ydcH OKGIIK_01135 0.30 -0.2 243,481 + ydcH OKGIIK_01135 0.30 +0.5 243,481 + ydcH OKGIIK_01135 0.30 +0.1 243,481 + ydcH OKGIIK_01135 0.30 -1.1 243,481 + ydcH OKGIIK_01135 0.30 +0.4 243,482 - ydcH OKGIIK_01135 0.31 +1.2 243,482 - ydcH OKGIIK_01135 0.31 -0.8 243,482 - ydcH OKGIIK_01135 0.31 -1.2 243,685 - +0.2 243,685 - -0.6 243,740 + +0.1 243,740 + -1.0 243,741 - -0.1 243,838 + -0.6 243,838 + +0.2 244,175 + aF2118 OKGIIK_01140 0.27 -0.0 244,175 + aF2118 OKGIIK_01140 0.27 -0.9 244,175 + aF2118 OKGIIK_01140 0.27 +0.8 244,175 + aF2118 OKGIIK_01140 0.27 -0.1 244,176 - aF2118 OKGIIK_01140 0.27 -0.6 244,176 - aF2118 OKGIIK_01140 0.27 -0.8 244,176 - aF2118 OKGIIK_01140 0.27 +0.1 244,176 - aF2118 OKGIIK_01140 0.27 -0.8 244,176 - aF2118 OKGIIK_01140 0.27 -0.6 244,178 + aF2118 OKGIIK_01140 0.28 -1.6 244,178 + aF2118 OKGIIK_01140 0.28 +1.4 244,178 + aF2118 OKGIIK_01140 0.28 -0.1 244,178 + aF2118 OKGIIK_01140 0.28 -1.6 244,179 - aF2118 OKGIIK_01140 0.28 -0.4 244,179 - aF2118 OKGIIK_01140 0.28 +0.4 244,179 - aF2118 OKGIIK_01140 0.28 +0.2 244,179 - aF2118 OKGIIK_01140 0.28 +1.1 244,179 - aF2118 OKGIIK_01140 0.28 -0.5 244,223 + aF2118 OKGIIK_01140 0.31 -0.6 244,223 + aF2118 OKGIIK_01140 0.31 -0.9 244,224 - aF2118 OKGIIK_01140 0.31 +0.2 244,224 - aF2118 OKGIIK_01140 0.31 -0.6 244,224 - aF2118 OKGIIK_01140 0.31 -0.1 244,224 - aF2118 OKGIIK_01140 0.31 -0.1 244,224 - aF2118 OKGIIK_01140 0.31 -0.4 244,439 + aF2118 OKGIIK_01140 0.47 -0.5 244,439 + aF2118 OKGIIK_01140 0.47 +0.6 244,439 + aF2118 OKGIIK_01140 0.47 -0.6 244,439 + aF2118 OKGIIK_01140 0.47 +0.7 244,439 + aF2118 OKGIIK_01140 0.47 -1.3 244,439 + aF2118 OKGIIK_01140 0.47 -1.9 244,439 + aF2118 OKGIIK_01140 0.47 +0.8 244,439 + aF2118 OKGIIK_01140 0.47 +0.2 244,439 + aF2118 OKGIIK_01140 0.47 +1.2 244,439 + aF2118 OKGIIK_01140 0.47 +0.0 244,439 + aF2118 OKGIIK_01140 0.47 +1.2 244,439 + aF2118 OKGIIK_01140 0.47 +1.4 244,440 - aF2118 OKGIIK_01140 0.47 +1.0 244,440 - aF2118 OKGIIK_01140 0.47 +0.3 244,440 - aF2118 OKGIIK_01140 0.47 +0.8 244,440 - aF2118 OKGIIK_01140 0.47 +0.6 244,440 - aF2118 OKGIIK_01140 0.47 +0.1 244,440 - aF2118 OKGIIK_01140 0.47 -0.1 244,440 - aF2118 OKGIIK_01140 0.47 +0.9 244,440 - aF2118 OKGIIK_01140 0.47 +0.9 244,440 - aF2118 OKGIIK_01140 0.47 +0.4 244,440 - aF2118 OKGIIK_01140 0.47 +0.2 244,440 - aF2118 OKGIIK_01140 0.47 +0.6 244,440 - aF2118 OKGIIK_01140 0.47 +1.9 244,440 - aF2118 OKGIIK_01140 0.47 +1.4 244,440 - aF2118 OKGIIK_01140 0.47 +2.6 244,547 + aF2118 OKGIIK_01140 0.54 -0.4 244,547 + aF2118 OKGIIK_01140 0.54 -0.2 244,547 + aF2118 OKGIIK_01140 0.54 +0.8 244,547 + aF2118 OKGIIK_01140 0.54 -0.4 244,547 + aF2118 OKGIIK_01140 0.54 +0.7 244,548 - aF2118 OKGIIK_01140 0.55 +0.4 244,548 - aF2118 OKGIIK_01140 0.55 +2.7 244,548 - aF2118 OKGIIK_01140 0.55 +1.5 244,548 - aF2118 OKGIIK_01140 0.55 -0.6 244,548 - aF2118 OKGIIK_01140 0.55 +0.7 244,548 - aF2118 OKGIIK_01140 0.55 -1.3 244,548 - aF2118 OKGIIK_01140 0.55 -0.4 244,655 + aF2118 OKGIIK_01140 0.62 -1.4 244,656 - aF2118 OKGIIK_01140 0.62 +0.4 244,656 - aF2118 OKGIIK_01140 0.62 +1.0 244,718 + aF2118 OKGIIK_01140 0.67 +0.7 244,718 + aF2118 OKGIIK_01140 0.67 +1.2 244,718 + aF2118 OKGIIK_01140 0.67 -1.6 244,718 + aF2118 OKGIIK_01140 0.67 -1.9 244,718 + aF2118 OKGIIK_01140 0.67 -0.2 244,718 + aF2118 OKGIIK_01140 0.67 -0.5 244,718 + aF2118 OKGIIK_01140 0.67 +0.2 244,718 + aF2118 OKGIIK_01140 0.67 +0.8 244,718 + aF2118 OKGIIK_01140 0.67 +0.5 244,718 + aF2118 OKGIIK_01140 0.67 -0.4 244,718 + aF2118 OKGIIK_01140 0.67 -0.3 244,718 + aF2118 OKGIIK_01140 0.67 +1.1 244,718 + aF2118 OKGIIK_01140 0.67 +0.2 244,719 - aF2118 OKGIIK_01140 0.67 +3.9 244,719 - aF2118 OKGIIK_01140 0.67 +0.1 244,719 - aF2118 OKGIIK_01140 0.67 -1.1 244,719 - aF2118 OKGIIK_01140 0.67 -1.0 244,719 - aF2118 OKGIIK_01140 0.67 -0.9 244,719 - aF2118 OKGIIK_01140 0.67 +0.4 244,719 - aF2118 OKGIIK_01140 0.67 +0.6 244,719 - aF2118 OKGIIK_01140 0.67 +0.2 244,719 - aF2118 OKGIIK_01140 0.67 +0.2 244,719 - aF2118 OKGIIK_01140 0.67 +0.2 244,719 - aF2118 OKGIIK_01140 0.67 +1.2 244,719 - aF2118 OKGIIK_01140 0.67 +0.9 244,733 + aF2118 OKGIIK_01140 0.68 -0.8 244,733 + aF2118 OKGIIK_01140 0.68 -0.5 244,733 + aF2118 OKGIIK_01140 0.68 +0.9 244,733 + aF2118 OKGIIK_01140 0.68 -1.8 244,733 + aF2118 OKGIIK_01140 0.68 +1.5 244,733 + aF2118 OKGIIK_01140 0.68 +0.2 244,733 + aF2118 OKGIIK_01140 0.68 +0.2 244,733 + aF2118 OKGIIK_01140 0.68 +1.2 244,734 - aF2118 OKGIIK_01140 0.68 +0.8 244,734 - aF2118 OKGIIK_01140 0.68 +0.2 244,734 - aF2118 OKGIIK_01140 0.68 +0.8 244,734 - aF2118 OKGIIK_01140 0.68 +0.9 244,734 - aF2118 OKGIIK_01140 0.68 -1.1 244,734 - aF2118 OKGIIK_01140 0.68 +1.0 244,742 + aF2118 OKGIIK_01140 0.69 -0.1 244,742 + aF2118 OKGIIK_01140 0.69 -1.2 244,743 - aF2118 OKGIIK_01140 0.69 +0.1 244,743 - aF2118 OKGIIK_01140 0.69 +0.2 244,868 + aF2118 OKGIIK_01140 0.78 -0.1 244,868 + aF2118 OKGIIK_01140 0.78 -1.4 244,868 + aF2118 OKGIIK_01140 0.78 +0.5 244,868 + aF2118 OKGIIK_01140 0.78 -0.4 244,868 + aF2118 OKGIIK_01140 0.78 +0.3 244,868 + aF2118 OKGIIK_01140 0.78 +0.1 244,869 - aF2118 OKGIIK_01140 0.78 +1.0 244,869 - aF2118 OKGIIK_01140 0.78 -1.4 244,869 - aF2118 OKGIIK_01140 0.78 -1.1 244,869 - aF2118 OKGIIK_01140 0.78 +0.7 244,869 - aF2118 OKGIIK_01140 0.78 -0.7 244,869 - aF2118 OKGIIK_01140 0.78 -0.4 244,886 + aF2118 OKGIIK_01140 0.79 +2.0 244,886 + aF2118 OKGIIK_01140 0.79 +0.6 244,886 + aF2118 OKGIIK_01140 0.79 +0.4 244,886 + aF2118 OKGIIK_01140 0.79 +0.2 244,886 + aF2118 OKGIIK_01140 0.79 -0.4 244,886 + aF2118 OKGIIK_01140 0.79 +0.7 244,886 + aF2118 OKGIIK_01140 0.79 +0.6 244,887 - aF2118 OKGIIK_01140 0.79 +1.4 244,887 - aF2118 OKGIIK_01140 0.79 +0.2 244,887 - aF2118 OKGIIK_01140 0.79 +1.0 244,887 - aF2118 OKGIIK_01140 0.79 -2.0 244,887 - aF2118 OKGIIK_01140 0.79 +0.5 245,147 + -0.4 245,147 + -1.0 245,147 + -0.6 245,147 + +0.3 245,147 + +0.5 245,147 + +0.4 245,148 - -1.1 245,148 - +0.0 245,148 - +1.6 245,148 - -0.4 245,148 - +0.8 245,148 - -0.8 245,148 - +1.0 245,148 - -0.8 245,148 - +1.6 245,148 - +1.5 245,148 - -0.1 245,148 - -0.4 245,168 + -1.1 245,168 + -1.5 245,168 + +0.2 245,168 + -1.6 245,168 + -0.8 245,168 + +0.5 245,168 + -2.5 245,168 + -0.1 245,169 - -0.2 245,169 - -0.5 245,169 - -0.4 245,169 - +1.0 245,169 - +0.6 245,169 - -0.8 245,169 - -0.2 245,169 - -1.0 245,169 - +0.2
Or see this region's nucleotide sequence