Experiment: Experiment:TE, Melon 2 Main Stem (TE-S-M2-MS)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS00405 and LU632_RS00410 are separated by 121 nucleotides LU632_RS00410 and LU632_RS00415 overlap by 20 nucleotides LU632_RS00415 and LU632_RS00420 overlap by 14 nucleotides LU632_RS00420 and sctJ overlap by 4 nucleotides
LU632_RS00405: LU632_RS00405 - IS5 family transposase, at 85,570 to 86,375
_RS00405
LU632_RS00410: LU632_RS00410 - hypothetical protein, at 86,497 to 86,919
_RS00410
LU632_RS00415: LU632_RS00415 - type III secretion protein, at 86,900 to 87,634
_RS00415
LU632_RS00420: LU632_RS00420 - type III secretion system protein, at 87,621 to 88,175
_RS00420
LU632_RS00425: sctJ - type III secretion inner membrane ring lipoprotein SctJ, at 88,172 to 88,942
sctJ
Position (kb) 86
87
88 Strain fitness (log2 ratio) -2
-1
0
1 at 86.503 kb on + strand at 86.569 kb on + strand, within LU632_RS00410 at 86.586 kb on + strand, within LU632_RS00410 at 86.591 kb on + strand, within LU632_RS00410 at 86.616 kb on - strand, within LU632_RS00410 at 87.626 kb on + strand at 87.770 kb on + strand, within LU632_RS00420 at 87.770 kb on + strand, within LU632_RS00420 at 87.960 kb on + strand, within LU632_RS00420 at 88.556 kb on - strand, within sctJ at 88.619 kb on - strand, within sctJ
Per-strain Table
Position Strand Gene LocusTag Fraction Experiment:TE, Melon 2 Main Stem (TE-S-M2-MS) remove 86,503 + +0.8 86,569 + LU632_RS00410 0.17 -0.6 86,586 + LU632_RS00410 0.21 +1.4 86,591 + LU632_RS00410 0.22 -1.9 86,616 - LU632_RS00410 0.28 -2.0 87,626 + +0.8 87,770 + LU632_RS00420 0.27 -0.5 87,770 + LU632_RS00420 0.27 -1.3 87,960 + LU632_RS00420 0.61 +0.2 88,556 - sctJ LU632_RS00425 0.50 -0.2 88,619 - sctJ LU632_RS00425 0.58 -2.4
Or see this region's nucleotide sequence