Strain Fitness in Escherichia coli ECRC62 around BNILDI_22495

Experiment: Bas61

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthemH and adk are separated by 131 nucleotidesadk and htpG are separated by 120 nucleotides BNILDI_22490: hemH - ferrochelatase, at 4,523,794 to 4,524,756 hemH BNILDI_22495: adk - adenylate kinase, at 4,524,888 to 4,525,592 adk BNILDI_22500: htpG - molecular chaperone HtpG, at 4,525,713 to 4,527,587 htpG Position (kb) 4524 4525 4526Strain fitness (log2 ratio) -1 0 1at 4524.817 kb on + strandat 4524.833 kb on + strandat 4526.135 kb on + strand, within htpGat 4526.136 kb on - strand, within htpGat 4526.136 kb on - strand, within htpGat 4526.177 kb on - strand, within htpGat 4526.177 kb on - strand, within htpGat 4526.234 kb on + strand, within htpGat 4526.276 kb on - strand, within htpGat 4526.276 kb on - strand, within htpGat 4526.293 kb on + strand, within htpGat 4526.293 kb on + strand, within htpGat 4526.297 kb on - strand, within htpGat 4526.332 kb on + strand, within htpGat 4526.333 kb on - strand, within htpGat 4526.333 kb on - strand, within htpGat 4526.375 kb on + strand, within htpGat 4526.376 kb on - strand, within htpGat 4526.445 kb on + strand, within htpGat 4526.477 kb on - strand, within htpG

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas61
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4,524,817 + +0.5
4,524,833 + +0.1
4,526,135 + htpG BNILDI_22500 0.23 +0.5
4,526,136 - htpG BNILDI_22500 0.23 -0.4
4,526,136 - htpG BNILDI_22500 0.23 -0.0
4,526,177 - htpG BNILDI_22500 0.25 -0.1
4,526,177 - htpG BNILDI_22500 0.25 +0.6
4,526,234 + htpG BNILDI_22500 0.28 -0.4
4,526,276 - htpG BNILDI_22500 0.30 -1.1
4,526,276 - htpG BNILDI_22500 0.30 -0.1
4,526,293 + htpG BNILDI_22500 0.31 +0.9
4,526,293 + htpG BNILDI_22500 0.31 +0.6
4,526,297 - htpG BNILDI_22500 0.31 -0.1
4,526,332 + htpG BNILDI_22500 0.33 -0.3
4,526,333 - htpG BNILDI_22500 0.33 -0.7
4,526,333 - htpG BNILDI_22500 0.33 +0.3
4,526,375 + htpG BNILDI_22500 0.35 +0.7
4,526,376 - htpG BNILDI_22500 0.35 -0.4
4,526,445 + htpG BNILDI_22500 0.39 -0.1
4,526,477 - htpG BNILDI_22500 0.41 -0.3

Or see this region's nucleotide sequence