Experiment: Bas61
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BNILDI_22225 and ybcJ are separated by 1 nucleotides ybcJ and ybcI are separated by 107 nucleotides ybcI and cysS are separated by 35 nucleotides
BNILDI_22225: BNILDI_22225 - bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD, at 4,466,559 to 4,467,425
_22225
BNILDI_22230: ybcJ - ribosome-associated protein YbcJ, at 4,467,427 to 4,467,639
ybcJ
BNILDI_22235: ybcI - Inner membrane protein YbcI, at 4,467,747 to 4,468,268
ybcI
BNILDI_22240: cysS - cysteine--tRNA ligase, at 4,468,304 to 4,469,689
cysS
Position (kb)
4467
4468
4469 Strain fitness (log2 ratio)
-1
0
1
2 at 4467.623 kb on + strand at 4467.624 kb on - strand at 4467.717 kb on + strand at 4467.717 kb on + strand at 4467.717 kb on + strand at 4467.718 kb on - strand at 4467.718 kb on - strand at 4467.762 kb on - strand at 4467.781 kb on + strand at 4467.781 kb on + strand at 4467.782 kb on - strand at 4467.829 kb on + strand, within ybcI at 4467.830 kb on - strand, within ybcI at 4467.871 kb on + strand, within ybcI at 4467.871 kb on + strand, within ybcI at 4467.872 kb on - strand, within ybcI at 4467.872 kb on - strand, within ybcI at 4467.965 kb on - strand, within ybcI
Per-strain Table
Position Strand Gene LocusTag Fraction Bas61 remove 4,467,623 + +1.4 4,467,624 - +2.1 4,467,717 + -0.6 4,467,717 + -0.2 4,467,717 + -0.9 4,467,718 - +0.1 4,467,718 - +1.1 4,467,762 - -0.7 4,467,781 + -0.5 4,467,781 + -0.4 4,467,782 - -0.6 4,467,829 + ybcI BNILDI_22235 0.16 +0.5 4,467,830 - ybcI BNILDI_22235 0.16 +1.1 4,467,871 + ybcI BNILDI_22235 0.24 +1.0 4,467,871 + ybcI BNILDI_22235 0.24 +1.9 4,467,872 - ybcI BNILDI_22235 0.24 +0.9 4,467,872 - ybcI BNILDI_22235 0.24 -0.0 4,467,965 - ybcI BNILDI_22235 0.42 +0.3
Or see this region's nucleotide sequence