Strain Fitness in Escherichia coli ECRC62 around BNILDI_22235

Experiment: Bas61

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBNILDI_22225 and ybcJ are separated by 1 nucleotidesybcJ and ybcI are separated by 107 nucleotidesybcI and cysS are separated by 35 nucleotides BNILDI_22225: BNILDI_22225 - bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD, at 4,466,559 to 4,467,425 _22225 BNILDI_22230: ybcJ - ribosome-associated protein YbcJ, at 4,467,427 to 4,467,639 ybcJ BNILDI_22235: ybcI - Inner membrane protein YbcI, at 4,467,747 to 4,468,268 ybcI BNILDI_22240: cysS - cysteine--tRNA ligase, at 4,468,304 to 4,469,689 cysS Position (kb) 4467 4468 4469Strain fitness (log2 ratio) -1 0 1 2at 4467.623 kb on + strandat 4467.624 kb on - strandat 4467.717 kb on + strandat 4467.717 kb on + strandat 4467.717 kb on + strandat 4467.718 kb on - strandat 4467.718 kb on - strandat 4467.762 kb on - strandat 4467.781 kb on + strandat 4467.781 kb on + strandat 4467.782 kb on - strandat 4467.829 kb on + strand, within ybcIat 4467.830 kb on - strand, within ybcIat 4467.871 kb on + strand, within ybcIat 4467.871 kb on + strand, within ybcIat 4467.872 kb on - strand, within ybcIat 4467.872 kb on - strand, within ybcIat 4467.965 kb on - strand, within ybcI

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas61
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4,467,623 + +1.4
4,467,624 - +2.1
4,467,717 + -0.6
4,467,717 + -0.2
4,467,717 + -0.9
4,467,718 - +0.1
4,467,718 - +1.1
4,467,762 - -0.7
4,467,781 + -0.5
4,467,781 + -0.4
4,467,782 - -0.6
4,467,829 + ybcI BNILDI_22235 0.16 +0.5
4,467,830 - ybcI BNILDI_22235 0.16 +1.1
4,467,871 + ybcI BNILDI_22235 0.24 +1.0
4,467,871 + ybcI BNILDI_22235 0.24 +1.9
4,467,872 - ybcI BNILDI_22235 0.24 +0.9
4,467,872 - ybcI BNILDI_22235 0.24 -0.0
4,467,965 - ybcI BNILDI_22235 0.42 +0.3

Or see this region's nucleotide sequence