Experiment: Bas61
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt potE and pgm are separated by 55 nucleotides pgm and seqA are separated by 25 nucleotides seqA and ybfF are separated by 184 nucleotides
BNILDI_21480: potE - putrescine-ornithine antiporter, at 4,322,785 to 4,324,104
potE
BNILDI_21485: pgm - phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent), at 4,324,160 to 4,325,800
pgm
BNILDI_21490: seqA - replication initiation negative regulator SeqA, at 4,325,826 to 4,326,371
seqA
BNILDI_21495: ybfF - esterase, at 4,326,556 to 4,327,320
ybfF
Position (kb)
4324
4325
4326 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4323.199 kb on - strand, within potE at 4323.240 kb on + strand, within potE at 4323.241 kb on - strand, within potE at 4323.313 kb on + strand, within potE at 4323.314 kb on - strand, within potE at 4323.360 kb on - strand, within potE at 4323.360 kb on - strand, within potE at 4323.376 kb on - strand, within potE at 4323.428 kb on + strand, within potE at 4323.429 kb on - strand, within potE at 4323.447 kb on + strand, within potE at 4323.447 kb on + strand, within potE at 4323.447 kb on + strand, within potE at 4323.448 kb on - strand, within potE at 4323.448 kb on - strand, within potE at 4323.480 kb on - strand, within potE at 4323.480 kb on - strand, within potE at 4323.497 kb on - strand, within potE at 4323.497 kb on - strand, within potE at 4323.534 kb on + strand, within potE at 4323.537 kb on + strand, within potE at 4323.538 kb on - strand, within potE at 4323.684 kb on + strand, within potE at 4323.778 kb on - strand, within potE at 4323.778 kb on - strand, within potE at 4323.875 kb on - strand, within potE at 4323.875 kb on - strand, within potE at 4323.875 kb on - strand, within potE at 4323.876 kb on + strand, within potE at 4323.896 kb on + strand, within potE at 4323.896 kb on + strand, within potE at 4323.976 kb on + strand at 4323.976 kb on + strand at 4323.977 kb on - strand at 4323.985 kb on + strand at 4323.994 kb on + strand at 4324.022 kb on - strand at 4324.057 kb on + strand at 4324.058 kb on - strand at 4324.162 kb on - strand at 4324.255 kb on + strand at 4324.255 kb on + strand at 4324.255 kb on + strand at 4324.255 kb on + strand at 4324.503 kb on + strand, within pgm at 4324.561 kb on + strand, within pgm at 4324.562 kb on - strand, within pgm at 4324.916 kb on - strand, within pgm at 4325.050 kb on - strand, within pgm at 4325.371 kb on + strand, within pgm at 4325.371 kb on + strand, within pgm at 4325.557 kb on - strand, within pgm at 4325.721 kb on + strand at 4325.721 kb on + strand at 4325.827 kb on + strand at 4326.076 kb on + strand, within seqA at 4326.078 kb on + strand, within seqA at 4326.131 kb on - strand, within seqA at 4326.279 kb on - strand, within seqA at 4326.279 kb on - strand, within seqA at 4326.334 kb on + strand at 4326.334 kb on + strand at 4326.335 kb on - strand at 4326.400 kb on + strand at 4326.401 kb on - strand at 4326.417 kb on - strand at 4326.417 kb on - strand at 4326.418 kb on + strand at 4326.418 kb on + strand at 4326.418 kb on + strand at 4326.425 kb on - strand at 4326.483 kb on - strand at 4326.516 kb on + strand at 4326.517 kb on - strand at 4326.517 kb on - strand at 4326.517 kb on - strand at 4326.519 kb on + strand at 4326.520 kb on - strand at 4326.688 kb on - strand, within ybfF at 4326.703 kb on - strand, within ybfF at 4326.775 kb on - strand, within ybfF
Per-strain Table
Position Strand Gene LocusTag Fraction Bas61 remove 4,323,199 - potE BNILDI_21480 0.31 -0.2 4,323,240 + potE BNILDI_21480 0.34 -1.9 4,323,241 - potE BNILDI_21480 0.35 -0.9 4,323,313 + potE BNILDI_21480 0.40 +0.2 4,323,314 - potE BNILDI_21480 0.40 -0.2 4,323,360 - potE BNILDI_21480 0.44 -0.2 4,323,360 - potE BNILDI_21480 0.44 +0.8 4,323,376 - potE BNILDI_21480 0.45 -0.2 4,323,428 + potE BNILDI_21480 0.49 -0.1 4,323,429 - potE BNILDI_21480 0.49 -0.1 4,323,447 + potE BNILDI_21480 0.50 -0.6 4,323,447 + potE BNILDI_21480 0.50 +0.5 4,323,447 + potE BNILDI_21480 0.50 +0.9 4,323,448 - potE BNILDI_21480 0.50 +1.6 4,323,448 - potE BNILDI_21480 0.50 -0.9 4,323,480 - potE BNILDI_21480 0.53 -0.3 4,323,480 - potE BNILDI_21480 0.53 -0.2 4,323,497 - potE BNILDI_21480 0.54 -0.9 4,323,497 - potE BNILDI_21480 0.54 -0.3 4,323,534 + potE BNILDI_21480 0.57 -0.9 4,323,537 + potE BNILDI_21480 0.57 -0.2 4,323,538 - potE BNILDI_21480 0.57 -0.4 4,323,684 + potE BNILDI_21480 0.68 +0.2 4,323,778 - potE BNILDI_21480 0.75 +1.1 4,323,778 - potE BNILDI_21480 0.75 +0.6 4,323,875 - potE BNILDI_21480 0.83 -0.3 4,323,875 - potE BNILDI_21480 0.83 -0.3 4,323,875 - potE BNILDI_21480 0.83 -1.1 4,323,876 + potE BNILDI_21480 0.83 -0.4 4,323,896 + potE BNILDI_21480 0.84 -0.3 4,323,896 + potE BNILDI_21480 0.84 -0.6 4,323,976 + -0.5 4,323,976 + +0.9 4,323,977 - +0.0 4,323,985 + +0.3 4,323,994 + +0.4 4,324,022 - -1.1 4,324,057 + +0.6 4,324,058 - +0.7 4,324,162 - -0.4 4,324,255 + +0.3 4,324,255 + -1.4 4,324,255 + -0.1 4,324,255 + +0.9 4,324,503 + pgm BNILDI_21485 0.21 -1.4 4,324,561 + pgm BNILDI_21485 0.24 +0.9 4,324,562 - pgm BNILDI_21485 0.24 +0.9 4,324,916 - pgm BNILDI_21485 0.46 -0.9 4,325,050 - pgm BNILDI_21485 0.54 +1.9 4,325,371 + pgm BNILDI_21485 0.74 +0.9 4,325,371 + pgm BNILDI_21485 0.74 +0.9 4,325,557 - pgm BNILDI_21485 0.85 +1.9 4,325,721 + -0.1 4,325,721 + -0.4 4,325,827 + -0.2 4,326,076 + seqA BNILDI_21490 0.46 +1.9 4,326,078 + seqA BNILDI_21490 0.46 +0.0 4,326,131 - seqA BNILDI_21490 0.56 +2.5 4,326,279 - seqA BNILDI_21490 0.83 -1.1 4,326,279 - seqA BNILDI_21490 0.83 -1.3 4,326,334 + +0.6 4,326,334 + -0.4 4,326,335 - -2.0 4,326,400 + +1.9 4,326,401 - -2.2 4,326,417 - +1.1 4,326,417 - -0.4 4,326,418 + +0.9 4,326,418 + -0.4 4,326,418 + +2.1 4,326,425 - +1.3 4,326,483 - -1.7 4,326,516 + +0.1 4,326,517 - +0.6 4,326,517 - -0.4 4,326,517 - +0.1 4,326,519 + +0.9 4,326,520 - -0.6 4,326,688 - ybfF BNILDI_21495 0.17 +1.9 4,326,703 - ybfF BNILDI_21495 0.19 -0.9 4,326,775 - ybfF BNILDI_21495 0.29 -0.1
Or see this region's nucleotide sequence