Strain Fitness in Escherichia coli ECRC62 around BNILDI_21000

Experiment: Bas61

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmoaE and moaD are separated by 1 nucleotidesmoaD and moaC overlap by 8 nucleotidesmoaC and moaB are separated by 2 nucleotidesmoaB and moaA are separated by 21 nucleotides BNILDI_20985: moaE - molybdopterin synthase catalytic subunit MoaE, at 4,224,591 to 4,225,043 moaE BNILDI_20990: moaD - molybdopterin synthase sulfur carrier subunit, at 4,225,045 to 4,225,290 moaD BNILDI_20995: moaC - cyclic pyranopterin monophosphate synthase MoaC, at 4,225,283 to 4,225,768 moaC BNILDI_21000: moaB - molybdenum cofactor biosynthesis protein B, at 4,225,771 to 4,226,283 moaB BNILDI_21005: moaA - GTP 3',8-cyclase MoaA, at 4,226,305 to 4,227,294 moaA Position (kb) 4225 4226 4227Strain fitness (log2 ratio) -1 0 1 2at 4225.361 kb on - strand, within moaCat 4225.390 kb on - strand, within moaCat 4225.464 kb on - strand, within moaCat 4225.464 kb on - strand, within moaCat 4225.607 kb on + strand, within moaCat 4225.608 kb on - strand, within moaCat 4225.636 kb on + strand, within moaCat 4225.769 kb on - strandat 4225.769 kb on - strandat 4225.769 kb on - strandat 4225.772 kb on + strandat 4225.772 kb on + strandat 4225.773 kb on - strandat 4225.773 kb on - strandat 4225.773 kb on - strandat 4226.065 kb on + strand, within moaBat 4226.074 kb on + strand, within moaBat 4226.101 kb on + strand, within moaBat 4226.146 kb on - strand, within moaBat 4226.272 kb on + strandat 4226.273 kb on - strandat 4226.318 kb on + strandat 4226.318 kb on + strandat 4226.338 kb on - strandat 4226.550 kb on + strand, within moaAat 4226.617 kb on + strand, within moaAat 4226.644 kb on + strand, within moaAat 4226.877 kb on + strand, within moaAat 4226.878 kb on - strand, within moaAat 4226.934 kb on + strand, within moaAat 4227.153 kb on + strand, within moaAat 4227.252 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas61
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4,225,361 - moaC BNILDI_20995 0.16 +0.3
4,225,390 - moaC BNILDI_20995 0.22 -0.5
4,225,464 - moaC BNILDI_20995 0.37 +1.0
4,225,464 - moaC BNILDI_20995 0.37 +0.3
4,225,607 + moaC BNILDI_20995 0.67 -1.5
4,225,608 - moaC BNILDI_20995 0.67 -0.1
4,225,636 + moaC BNILDI_20995 0.73 -1.2
4,225,769 - +2.1
4,225,769 - -0.7
4,225,769 - -0.9
4,225,772 + -0.0
4,225,772 + -1.4
4,225,773 - -0.4
4,225,773 - -1.3
4,225,773 - -0.5
4,226,065 + moaB BNILDI_21000 0.57 +1.4
4,226,074 + moaB BNILDI_21000 0.59 +0.1
4,226,101 + moaB BNILDI_21000 0.64 +1.7
4,226,146 - moaB BNILDI_21000 0.73 -0.3
4,226,272 + +0.3
4,226,273 - -0.7
4,226,318 + -1.5
4,226,318 + -0.5
4,226,338 - -1.3
4,226,550 + moaA BNILDI_21005 0.25 +0.4
4,226,617 + moaA BNILDI_21005 0.32 -0.7
4,226,644 + moaA BNILDI_21005 0.34 -1.3
4,226,877 + moaA BNILDI_21005 0.58 +0.3
4,226,878 - moaA BNILDI_21005 0.58 -0.4
4,226,934 + moaA BNILDI_21005 0.64 -0.3
4,227,153 + moaA BNILDI_21005 0.86 -0.1
4,227,252 - +0.1

Or see this region's nucleotide sequence