Strain Fitness in Escherichia coli ECRC62 around BNILDI_20860

Experiment: Bas61

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntybiO and rlmF overlap by 4 nucleotidesrlmF and mcbA are separated by 275 nucleotidesmcbA and fiu are separated by 263 nucleotides BNILDI_20855: ybiO - mechanosensitive channel protein, at 4,198,859 to 4,201,084 ybiO BNILDI_20860: rlmF - 23S rRNA (adenine(1618)-N(6))-methyltransferase RlmF, at 4,201,081 to 4,202,007 rlmF BNILDI_20865: mcbA - DUF1471 domain-containing protein, at 4,202,283 to 4,202,543 mcbA BNILDI_20870: fiu - catecholate siderophore receptor Fiu, at 4,202,807 to 4,205,089 fiu Position (kb) 4201 4202 4203Strain fitness (log2 ratio) -2 -1 0 1 2at 4200.088 kb on + strand, within ybiOat 4200.151 kb on + strand, within ybiOat 4200.249 kb on + strand, within ybiOat 4200.249 kb on + strand, within ybiOat 4200.249 kb on + strand, within ybiOat 4200.250 kb on - strand, within ybiOat 4200.250 kb on - strand, within ybiOat 4200.255 kb on - strand, within ybiOat 4200.424 kb on + strand, within ybiOat 4200.498 kb on + strand, within ybiOat 4200.498 kb on + strand, within ybiOat 4200.499 kb on - strand, within ybiOat 4200.749 kb on + strand, within ybiOat 4200.778 kb on + strand, within ybiOat 4200.883 kb on - strandat 4200.883 kb on - strandat 4201.016 kb on + strandat 4201.017 kb on - strandat 4201.017 kb on - strandat 4201.017 kb on - strandat 4201.218 kb on - strand, within rlmFat 4201.247 kb on - strand, within rlmFat 4201.258 kb on - strand, within rlmFat 4201.414 kb on - strand, within rlmFat 4201.414 kb on - strand, within rlmFat 4201.631 kb on + strand, within rlmFat 4201.632 kb on - strand, within rlmFat 4201.639 kb on + strand, within rlmFat 4201.640 kb on - strand, within rlmFat 4201.640 kb on - strand, within rlmFat 4201.640 kb on - strand, within rlmFat 4201.660 kb on - strand, within rlmFat 4201.660 kb on - strand, within rlmFat 4201.660 kb on - strand, within rlmFat 4201.750 kb on - strand, within rlmFat 4201.751 kb on + strand, within rlmFat 4201.752 kb on - strand, within rlmFat 4201.752 kb on - strand, within rlmFat 4201.840 kb on - strand, within rlmFat 4201.840 kb on - strand, within rlmFat 4202.008 kb on - strandat 4202.008 kb on - strandat 4202.020 kb on + strandat 4202.020 kb on + strandat 4202.032 kb on + strandat 4202.063 kb on - strandat 4202.240 kb on - strandat 4202.240 kb on - strandat 4202.240 kb on - strandat 4202.240 kb on - strandat 4202.245 kb on + strandat 4202.246 kb on - strandat 4202.246 kb on - strandat 4202.264 kb on + strandat 4202.264 kb on + strandat 4202.265 kb on - strandat 4202.265 kb on - strandat 4202.268 kb on + strandat 4202.268 kb on + strandat 4202.364 kb on - strand, within mcbAat 4202.490 kb on - strand, within mcbAat 4202.555 kb on + strandat 4202.719 kb on - strandat 4202.730 kb on - strandat 4202.730 kb on - strandat 4202.785 kb on + strandat 4202.872 kb on + strandat 4202.872 kb on + strandat 4202.873 kb on - strandat 4202.966 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas61
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4,200,088 + ybiO BNILDI_20855 0.55 -0.3
4,200,151 + ybiO BNILDI_20855 0.58 +0.1
4,200,249 + ybiO BNILDI_20855 0.62 -2.1
4,200,249 + ybiO BNILDI_20855 0.62 -0.2
4,200,249 + ybiO BNILDI_20855 0.62 +0.4
4,200,250 - ybiO BNILDI_20855 0.62 -1.3
4,200,250 - ybiO BNILDI_20855 0.62 +0.6
4,200,255 - ybiO BNILDI_20855 0.63 -0.3
4,200,424 + ybiO BNILDI_20855 0.70 -1.6
4,200,498 + ybiO BNILDI_20855 0.74 -1.9
4,200,498 + ybiO BNILDI_20855 0.74 -0.1
4,200,499 - ybiO BNILDI_20855 0.74 -1.3
4,200,749 + ybiO BNILDI_20855 0.85 +1.1
4,200,778 + ybiO BNILDI_20855 0.86 +0.4
4,200,883 - +1.3
4,200,883 - -1.1
4,201,016 + -0.8
4,201,017 - +0.2
4,201,017 - -0.5
4,201,017 - +0.1
4,201,218 - rlmF BNILDI_20860 0.15 -1.5
4,201,247 - rlmF BNILDI_20860 0.18 +0.3
4,201,258 - rlmF BNILDI_20860 0.19 +0.4
4,201,414 - rlmF BNILDI_20860 0.36 +0.3
4,201,414 - rlmF BNILDI_20860 0.36 +1.3
4,201,631 + rlmF BNILDI_20860 0.59 -0.7
4,201,632 - rlmF BNILDI_20860 0.59 +0.7
4,201,639 + rlmF BNILDI_20860 0.60 -0.3
4,201,640 - rlmF BNILDI_20860 0.60 +0.3
4,201,640 - rlmF BNILDI_20860 0.60 +0.0
4,201,640 - rlmF BNILDI_20860 0.60 +0.7
4,201,660 - rlmF BNILDI_20860 0.62 +0.9
4,201,660 - rlmF BNILDI_20860 0.62 -0.3
4,201,660 - rlmF BNILDI_20860 0.62 -0.6
4,201,750 - rlmF BNILDI_20860 0.72 +0.3
4,201,751 + rlmF BNILDI_20860 0.72 -0.3
4,201,752 - rlmF BNILDI_20860 0.72 +1.0
4,201,752 - rlmF BNILDI_20860 0.72 +0.4
4,201,840 - rlmF BNILDI_20860 0.82 -1.1
4,201,840 - rlmF BNILDI_20860 0.82 -1.1
4,202,008 - +1.9
4,202,008 - -0.7
4,202,020 + -0.7
4,202,020 + -0.1
4,202,032 + -0.1
4,202,063 - -0.5
4,202,240 - -0.3
4,202,240 - +0.5
4,202,240 - +0.7
4,202,240 - +1.3
4,202,245 + +0.3
4,202,246 - -0.2
4,202,246 - -0.8
4,202,264 + +1.4
4,202,264 + -0.4
4,202,265 - -0.1
4,202,265 - +0.0
4,202,268 + -1.8
4,202,268 + -0.4
4,202,364 - mcbA BNILDI_20865 0.31 -0.5
4,202,490 - mcbA BNILDI_20865 0.79 +0.4
4,202,555 + -0.3
4,202,719 - +0.2
4,202,730 - -0.6
4,202,730 - -1.1
4,202,785 + -0.3
4,202,872 + -0.7
4,202,872 + +1.6
4,202,873 - -0.5
4,202,966 + +0.1

Or see this region's nucleotide sequence