Strain Fitness in Escherichia coli ECRC62 around BNILDI_20850

Experiment: Bas61

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntglnH and glnP are separated by 138 nucleotidesglnP and glnQ overlap by 4 nucleotidesglnQ and ybiO are separated by 116 nucleotides BNILDI_20840: glnH - glutamine ABC transporter substrate-binding protein GlnH, at 4,196,479 to 4,197,225 glnH BNILDI_20845: glnP - glutamine ABC transporter permease GlnP, at 4,197,364 to 4,198,023 glnP BNILDI_20850: glnQ - glutamine ABC transporter ATP-binding protein GlnQ, at 4,198,020 to 4,198,742 glnQ BNILDI_20855: ybiO - mechanosensitive channel protein, at 4,198,859 to 4,201,084 ybiO Position (kb) 4198 4199Strain fitness (log2 ratio) -2 -1 0 1 2at 4197.181 kb on + strandat 4197.232 kb on + strandat 4197.240 kb on + strandat 4197.241 kb on - strandat 4197.282 kb on + strandat 4197.283 kb on - strandat 4197.283 kb on - strandat 4197.317 kb on - strandat 4197.321 kb on - strandat 4197.339 kb on + strandat 4197.339 kb on + strandat 4197.363 kb on + strandat 4197.364 kb on - strandat 4197.364 kb on - strandat 4197.364 kb on - strandat 4197.562 kb on - strand, within glnPat 4197.562 kb on - strand, within glnPat 4197.582 kb on + strand, within glnPat 4197.582 kb on + strand, within glnPat 4197.583 kb on - strand, within glnPat 4197.586 kb on + strand, within glnPat 4197.587 kb on - strand, within glnPat 4197.609 kb on + strand, within glnPat 4197.618 kb on - strand, within glnPat 4197.618 kb on - strand, within glnPat 4197.634 kb on - strand, within glnPat 4197.673 kb on + strand, within glnPat 4197.773 kb on - strand, within glnPat 4197.801 kb on + strand, within glnPat 4197.963 kb on + strandat 4197.985 kb on - strandat 4198.278 kb on + strand, within glnQat 4198.740 kb on + strandat 4198.740 kb on + strandat 4198.819 kb on + strandat 4198.820 kb on - strandat 4198.820 kb on - strandat 4198.822 kb on - strandat 4198.822 kb on - strandat 4198.822 kb on - strandat 4198.826 kb on - strandat 4198.931 kb on - strandat 4199.210 kb on + strand, within ybiOat 4199.236 kb on + strand, within ybiOat 4199.245 kb on + strand, within ybiOat 4199.298 kb on + strand, within ybiOat 4199.298 kb on + strand, within ybiOat 4199.298 kb on + strand, within ybiOat 4199.298 kb on + strand, within ybiOat 4199.432 kb on - strand, within ybiOat 4199.432 kb on - strand, within ybiOat 4199.463 kb on + strand, within ybiOat 4199.463 kb on + strand, within ybiOat 4199.464 kb on - strand, within ybiOat 4199.464 kb on - strand, within ybiOat 4199.479 kb on - strand, within ybiOat 4199.719 kb on + strand, within ybiOat 4199.733 kb on + strand, within ybiOat 4199.734 kb on - strand, within ybiOat 4199.740 kb on - strand, within ybiO

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas61
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4,197,181 + -0.5
4,197,232 + +0.1
4,197,240 + -0.7
4,197,241 - -0.3
4,197,282 + -0.5
4,197,283 - -0.5
4,197,283 - -1.9
4,197,317 - +0.7
4,197,321 - -0.7
4,197,339 + +1.2
4,197,339 + -0.7
4,197,363 + -0.1
4,197,364 - +0.9
4,197,364 - +1.9
4,197,364 - -0.5
4,197,562 - glnP BNILDI_20845 0.30 -0.1
4,197,562 - glnP BNILDI_20845 0.30 +0.6
4,197,582 + glnP BNILDI_20845 0.33 -1.8
4,197,582 + glnP BNILDI_20845 0.33 -0.1
4,197,583 - glnP BNILDI_20845 0.33 -0.3
4,197,586 + glnP BNILDI_20845 0.34 +0.7
4,197,587 - glnP BNILDI_20845 0.34 -1.3
4,197,609 + glnP BNILDI_20845 0.37 -0.4
4,197,618 - glnP BNILDI_20845 0.38 -0.1
4,197,618 - glnP BNILDI_20845 0.38 +2.3
4,197,634 - glnP BNILDI_20845 0.41 +1.1
4,197,673 + glnP BNILDI_20845 0.47 +1.1
4,197,773 - glnP BNILDI_20845 0.62 +0.2
4,197,801 + glnP BNILDI_20845 0.66 -0.4
4,197,963 + +0.1
4,197,985 - -0.4
4,198,278 + glnQ BNILDI_20850 0.36 +0.4
4,198,740 + +0.1
4,198,740 + -0.1
4,198,819 + -0.0
4,198,820 - -0.3
4,198,820 - -0.3
4,198,822 - -0.4
4,198,822 - +1.1
4,198,822 - +1.1
4,198,826 - -0.8
4,198,931 - -0.6
4,199,210 + ybiO BNILDI_20855 0.16 +1.2
4,199,236 + ybiO BNILDI_20855 0.17 -0.1
4,199,245 + ybiO BNILDI_20855 0.17 -1.9
4,199,298 + ybiO BNILDI_20855 0.20 -0.1
4,199,298 + ybiO BNILDI_20855 0.20 -0.0
4,199,298 + ybiO BNILDI_20855 0.20 -1.3
4,199,298 + ybiO BNILDI_20855 0.20 -0.7
4,199,432 - ybiO BNILDI_20855 0.26 -0.5
4,199,432 - ybiO BNILDI_20855 0.26 +0.9
4,199,463 + ybiO BNILDI_20855 0.27 -0.3
4,199,463 + ybiO BNILDI_20855 0.27 -0.2
4,199,464 - ybiO BNILDI_20855 0.27 +0.4
4,199,464 - ybiO BNILDI_20855 0.27 +1.0
4,199,479 - ybiO BNILDI_20855 0.28 +0.4
4,199,719 + ybiO BNILDI_20855 0.39 -0.5
4,199,733 + ybiO BNILDI_20855 0.39 -0.4
4,199,734 - ybiO BNILDI_20855 0.39 -1.0
4,199,740 - ybiO BNILDI_20855 0.40 +0.7

Or see this region's nucleotide sequence