Strain Fitness in Escherichia coli ECRC62 around BNILDI_19930

Experiment: Bas61

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyccT and mgsA are separated by 95 nucleotidesmgsA and helD are separated by 31 nucleotideshelD and yccF are separated by 122 nucleotidesyccF and yccS are separated by 9 nucleotides BNILDI_19920: yccT - DUF2057 domain-containing protein, at 3,996,427 to 3,997,089 yccT BNILDI_19925: mgsA - methylglyoxal synthase, at 3,997,185 to 3,997,643 mgsA BNILDI_19930: helD - DNA helicase IV, at 3,997,675 to 3,999,729 helD BNILDI_19935: yccF - YccF domain-containing protein, at 3,999,852 to 4,000,298 yccF BNILDI_19940: yccS - Inner membrane protein YccS, at 4,000,308 to 4,002,470 yccS Position (kb) 3997 3998 3999 4000Strain fitness (log2 ratio) -2 -1 0 1 2at 3996.940 kb on - strand, within yccTat 3996.972 kb on + strand, within yccTat 3996.972 kb on + strand, within yccTat 3997.016 kb on - strand, within yccTat 3997.126 kb on - strandat 3997.138 kb on + strandat 3997.138 kb on + strandat 3997.139 kb on - strandat 3997.146 kb on + strandat 3997.151 kb on + strandat 3997.167 kb on + strandat 3997.168 kb on - strandat 3997.168 kb on - strandat 3997.180 kb on - strandat 3997.306 kb on + strand, within mgsAat 3997.306 kb on + strand, within mgsAat 3997.307 kb on - strand, within mgsAat 3997.311 kb on + strand, within mgsAat 3997.463 kb on - strand, within mgsAat 3997.621 kb on - strandat 3997.621 kb on - strandat 3997.676 kb on + strandat 3997.790 kb on + strandat 3997.790 kb on + strandat 3997.790 kb on + strandat 3997.791 kb on - strandat 3997.791 kb on - strandat 3997.791 kb on - strandat 3997.957 kb on - strand, within helDat 3998.024 kb on + strand, within helDat 3998.225 kb on + strand, within helDat 3998.225 kb on + strand, within helDat 3998.236 kb on + strand, within helDat 3998.430 kb on + strand, within helDat 3998.565 kb on + strand, within helDat 3998.565 kb on + strand, within helDat 3998.651 kb on - strand, within helDat 3998.710 kb on - strand, within helDat 3998.710 kb on - strand, within helDat 3998.808 kb on + strand, within helDat 3998.884 kb on - strand, within helDat 3998.886 kb on + strand, within helDat 3998.887 kb on - strand, within helDat 3998.932 kb on - strand, within helDat 3998.932 kb on - strand, within helDat 3999.201 kb on - strand, within helDat 3999.201 kb on - strand, within helDat 3999.201 kb on - strand, within helDat 3999.210 kb on + strand, within helDat 3999.258 kb on + strand, within helDat 3999.324 kb on + strand, within helDat 3999.325 kb on - strand, within helDat 3999.325 kb on - strand, within helDat 3999.738 kb on - strandat 3999.769 kb on - strandat 3999.839 kb on - strandat 3999.851 kb on + strandat 3999.852 kb on - strandat 3999.936 kb on - strand, within yccFat 3999.938 kb on + strand, within yccFat 3999.938 kb on + strand, within yccFat 3999.939 kb on - strand, within yccFat 3999.939 kb on - strand, within yccFat 3999.939 kb on - strand, within yccFat 4000.081 kb on + strand, within yccFat 4000.322 kb on - strandat 4000.322 kb on - strandat 4000.365 kb on + strandat 4000.429 kb on + strandat 4000.429 kb on + strandat 4000.558 kb on + strand, within yccSat 4000.650 kb on + strand, within yccSat 4000.689 kb on + strand, within yccSat 4000.689 kb on + strand, within yccSat 4000.689 kb on + strand, within yccSat 4000.690 kb on - strand, within yccSat 4000.694 kb on + strand, within yccSat 4000.694 kb on + strand, within yccSat 4000.694 kb on + strand, within yccSat 4000.694 kb on + strand, within yccSat 4000.695 kb on - strand, within yccSat 4000.713 kb on + strand, within yccSat 4000.714 kb on - strand, within yccSat 4000.714 kb on - strand, within yccS

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas61
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3,996,940 - yccT BNILDI_19920 0.77 -0.2
3,996,972 + yccT BNILDI_19920 0.82 +0.8
3,996,972 + yccT BNILDI_19920 0.82 -0.2
3,997,016 - yccT BNILDI_19920 0.89 -0.8
3,997,126 - +0.2
3,997,138 + -0.2
3,997,138 + -0.4
3,997,139 - +0.8
3,997,146 + +0.6
3,997,151 + -0.6
3,997,167 + +0.8
3,997,168 - -0.4
3,997,168 - -0.6
3,997,180 - -0.1
3,997,306 + mgsA BNILDI_19925 0.26 -0.4
3,997,306 + mgsA BNILDI_19925 0.26 -0.6
3,997,307 - mgsA BNILDI_19925 0.27 +0.1
3,997,311 + mgsA BNILDI_19925 0.27 +0.4
3,997,463 - mgsA BNILDI_19925 0.61 -0.0
3,997,621 - -0.1
3,997,621 - -0.7
3,997,676 + +0.0
3,997,790 + +1.8
3,997,790 + -1.0
3,997,790 + +0.1
3,997,791 - -0.7
3,997,791 - -0.3
3,997,791 - -0.3
3,997,957 - helD BNILDI_19930 0.14 +1.8
3,998,024 + helD BNILDI_19930 0.17 -0.4
3,998,225 + helD BNILDI_19930 0.27 -0.4
3,998,225 + helD BNILDI_19930 0.27 +0.4
3,998,236 + helD BNILDI_19930 0.27 -0.5
3,998,430 + helD BNILDI_19930 0.37 +0.3
3,998,565 + helD BNILDI_19930 0.43 +0.1
3,998,565 + helD BNILDI_19930 0.43 -0.3
3,998,651 - helD BNILDI_19930 0.47 +0.0
3,998,710 - helD BNILDI_19930 0.50 -0.6
3,998,710 - helD BNILDI_19930 0.50 +0.3
3,998,808 + helD BNILDI_19930 0.55 +0.0
3,998,884 - helD BNILDI_19930 0.59 -1.8
3,998,886 + helD BNILDI_19930 0.59 +0.8
3,998,887 - helD BNILDI_19930 0.59 +0.1
3,998,932 - helD BNILDI_19930 0.61 -0.1
3,998,932 - helD BNILDI_19930 0.61 +1.8
3,999,201 - helD BNILDI_19930 0.74 +0.8
3,999,201 - helD BNILDI_19930 0.74 +0.8
3,999,201 - helD BNILDI_19930 0.74 -0.1
3,999,210 + helD BNILDI_19930 0.75 -1.0
3,999,258 + helD BNILDI_19930 0.77 -1.0
3,999,324 + helD BNILDI_19930 0.80 -0.5
3,999,325 - helD BNILDI_19930 0.80 -2.0
3,999,325 - helD BNILDI_19930 0.80 -0.4
3,999,738 - +0.6
3,999,769 - -0.8
3,999,839 - -1.5
3,999,851 + -1.2
3,999,852 - +0.0
3,999,936 - yccF BNILDI_19935 0.19 -0.0
3,999,938 + yccF BNILDI_19935 0.19 +0.2
3,999,938 + yccF BNILDI_19935 0.19 -0.8
3,999,939 - yccF BNILDI_19935 0.19 +0.6
3,999,939 - yccF BNILDI_19935 0.19 -0.4
3,999,939 - yccF BNILDI_19935 0.19 +0.6
4,000,081 + yccF BNILDI_19935 0.51 -0.4
4,000,322 - +0.2
4,000,322 - -2.1
4,000,365 + +0.8
4,000,429 + -1.1
4,000,429 + -0.4
4,000,558 + yccS BNILDI_19940 0.12 -0.6
4,000,650 + yccS BNILDI_19940 0.16 -0.9
4,000,689 + yccS BNILDI_19940 0.18 +1.1
4,000,689 + yccS BNILDI_19940 0.18 -0.6
4,000,689 + yccS BNILDI_19940 0.18 +1.8
4,000,690 - yccS BNILDI_19940 0.18 +0.4
4,000,694 + yccS BNILDI_19940 0.18 -0.3
4,000,694 + yccS BNILDI_19940 0.18 -0.2
4,000,694 + yccS BNILDI_19940 0.18 +0.5
4,000,694 + yccS BNILDI_19940 0.18 +0.5
4,000,695 - yccS BNILDI_19940 0.18 +0.8
4,000,713 + yccS BNILDI_19940 0.19 +0.6
4,000,714 - yccS BNILDI_19940 0.19 -1.4
4,000,714 - yccS BNILDI_19940 0.19 +0.0

Or see this region's nucleotide sequence