Experiment: Bas61
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt flgD and flgC are separated by 11 nucleotides flgC and flgB are separated by 3 nucleotides flgB and flgA are separated by 154 nucleotides flgA and flgM are separated by 75 nucleotides flgM and flgN are separated by 4 nucleotides flgN and murJ are separated by 39 nucleotides
BNILDI_19355: flgD - flagellar hook assembly protein FlgD, at 3,892,905 to 3,893,600
flgD
BNILDI_19360: flgC - flagellar basal body rod protein FlgC, at 3,893,612 to 3,894,016
flgC
BNILDI_19365: flgB - flagellar basal body rod protein FlgB, at 3,894,020 to 3,894,436
flgB
BNILDI_19370: flgA - flagellar basal body P-ring formation protein FlgA, at 3,894,591 to 3,895,250
flgA
BNILDI_19375: flgM - anti-sigma-28 factor FlgM, at 3,895,326 to 3,895,619
flgM
BNILDI_19380: flgN - flagella biosynthesis chaperone FlgN, at 3,895,624 to 3,896,040
flgN
BNILDI_19385: murJ - murein biosynthesis integral membrane protein MurJ, at 3,896,080 to 3,897,615
murJ
Position (kb)
3894
3895
3896 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3893.600 kb on + strand at 3893.601 kb on - strand at 3893.614 kb on - strand at 3893.745 kb on - strand, within flgC at 3893.745 kb on - strand, within flgC at 3893.813 kb on + strand, within flgC at 3893.814 kb on - strand, within flgC at 3893.831 kb on + strand, within flgC at 3893.873 kb on + strand, within flgC at 3893.874 kb on - strand, within flgC at 3894.001 kb on + strand at 3894.143 kb on - strand, within flgB at 3894.411 kb on - strand at 3894.436 kb on + strand at 3894.436 kb on + strand at 3894.474 kb on - strand at 3894.528 kb on + strand at 3894.602 kb on - strand at 3894.644 kb on - strand at 3894.647 kb on + strand at 3894.728 kb on + strand, within flgA at 3894.729 kb on - strand, within flgA at 3894.734 kb on + strand, within flgA at 3894.748 kb on + strand, within flgA at 3894.748 kb on + strand, within flgA at 3894.748 kb on + strand, within flgA at 3894.751 kb on + strand, within flgA at 3894.789 kb on - strand, within flgA at 3894.789 kb on - strand, within flgA at 3894.846 kb on + strand, within flgA at 3894.850 kb on + strand, within flgA at 3894.879 kb on + strand, within flgA at 3894.879 kb on + strand, within flgA at 3895.024 kb on + strand, within flgA at 3895.025 kb on - strand, within flgA at 3895.025 kb on - strand, within flgA at 3895.040 kb on + strand, within flgA at 3895.201 kb on + strand at 3895.233 kb on - strand at 3895.233 kb on - strand at 3895.239 kb on - strand at 3895.240 kb on + strand at 3895.249 kb on - strand at 3895.284 kb on + strand at 3895.284 kb on + strand at 3895.285 kb on - strand at 3895.285 kb on - strand at 3895.320 kb on + strand at 3895.331 kb on + strand at 3895.332 kb on - strand at 3895.523 kb on - strand, within flgM at 3895.549 kb on + strand, within flgM at 3895.614 kb on - strand at 3895.614 kb on - strand at 3895.623 kb on + strand at 3895.669 kb on - strand, within flgN at 3895.837 kb on - strand, within flgN at 3895.900 kb on - strand, within flgN at 3895.900 kb on - strand, within flgN at 3895.979 kb on + strand, within flgN at 3895.979 kb on + strand, within flgN at 3895.979 kb on + strand, within flgN at 3895.980 kb on - strand, within flgN at 3896.045 kb on + strand at 3896.081 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas61 remove 3,893,600 + +0.9 3,893,601 - +0.5 3,893,614 - +0.2 3,893,745 - flgC BNILDI_19360 0.33 -0.8 3,893,745 - flgC BNILDI_19360 0.33 +1.0 3,893,813 + flgC BNILDI_19360 0.50 -0.0 3,893,814 - flgC BNILDI_19360 0.50 +0.8 3,893,831 + flgC BNILDI_19360 0.54 +3.7 3,893,873 + flgC BNILDI_19360 0.64 +1.6 3,893,874 - flgC BNILDI_19360 0.65 +1.1 3,894,001 + -2.1 3,894,143 - flgB BNILDI_19365 0.29 +1.0 3,894,411 - +3.0 3,894,436 + +3.1 3,894,436 + +0.7 3,894,474 - -1.4 3,894,528 + +0.4 3,894,602 - +1.8 3,894,644 - -0.4 3,894,647 + -1.2 3,894,728 + flgA BNILDI_19370 0.21 +1.6 3,894,729 - flgA BNILDI_19370 0.21 -0.5 3,894,734 + flgA BNILDI_19370 0.22 +2.9 3,894,748 + flgA BNILDI_19370 0.24 +1.2 3,894,748 + flgA BNILDI_19370 0.24 +2.8 3,894,748 + flgA BNILDI_19370 0.24 +1.3 3,894,751 + flgA BNILDI_19370 0.24 +1.8 3,894,789 - flgA BNILDI_19370 0.30 +1.8 3,894,789 - flgA BNILDI_19370 0.30 -0.5 3,894,846 + flgA BNILDI_19370 0.39 +2.5 3,894,850 + flgA BNILDI_19370 0.39 -0.8 3,894,879 + flgA BNILDI_19370 0.44 +0.2 3,894,879 + flgA BNILDI_19370 0.44 +0.8 3,895,024 + flgA BNILDI_19370 0.66 +2.5 3,895,025 - flgA BNILDI_19370 0.66 -1.5 3,895,025 - flgA BNILDI_19370 0.66 +1.8 3,895,040 + flgA BNILDI_19370 0.68 -0.8 3,895,201 + +1.4 3,895,233 - -1.1 3,895,233 - +1.2 3,895,239 - +0.5 3,895,240 + -1.4 3,895,249 - +1.8 3,895,284 + -0.5 3,895,284 + -0.2 3,895,285 - +0.2 3,895,285 - -0.7 3,895,320 + +0.3 3,895,331 + -1.0 3,895,332 - -0.2 3,895,523 - flgM BNILDI_19375 0.67 +0.3 3,895,549 + flgM BNILDI_19375 0.76 -0.6 3,895,614 - +2.8 3,895,614 - +0.0 3,895,623 + +0.5 3,895,669 - flgN BNILDI_19380 0.11 -1.2 3,895,837 - flgN BNILDI_19380 0.51 +1.0 3,895,900 - flgN BNILDI_19380 0.66 +0.6 3,895,900 - flgN BNILDI_19380 0.66 +0.2 3,895,979 + flgN BNILDI_19380 0.85 +0.1 3,895,979 + flgN BNILDI_19380 0.85 -0.6 3,895,979 + flgN BNILDI_19380 0.85 -1.5 3,895,980 - flgN BNILDI_19380 0.85 +0.8 3,896,045 + +0.0 3,896,081 + -0.2
Or see this region's nucleotide sequence