Strain Fitness in Escherichia coli ECRC62 around BNILDI_19370

Experiment: Bas61

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntflgD and flgC are separated by 11 nucleotidesflgC and flgB are separated by 3 nucleotidesflgB and flgA are separated by 154 nucleotidesflgA and flgM are separated by 75 nucleotidesflgM and flgN are separated by 4 nucleotidesflgN and murJ are separated by 39 nucleotides BNILDI_19355: flgD - flagellar hook assembly protein FlgD, at 3,892,905 to 3,893,600 flgD BNILDI_19360: flgC - flagellar basal body rod protein FlgC, at 3,893,612 to 3,894,016 flgC BNILDI_19365: flgB - flagellar basal body rod protein FlgB, at 3,894,020 to 3,894,436 flgB BNILDI_19370: flgA - flagellar basal body P-ring formation protein FlgA, at 3,894,591 to 3,895,250 flgA BNILDI_19375: flgM - anti-sigma-28 factor FlgM, at 3,895,326 to 3,895,619 flgM BNILDI_19380: flgN - flagella biosynthesis chaperone FlgN, at 3,895,624 to 3,896,040 flgN BNILDI_19385: murJ - murein biosynthesis integral membrane protein MurJ, at 3,896,080 to 3,897,615 murJ Position (kb) 3894 3895 3896Strain fitness (log2 ratio) -2 -1 0 1 2 3at 3893.600 kb on + strandat 3893.601 kb on - strandat 3893.614 kb on - strandat 3893.745 kb on - strand, within flgCat 3893.745 kb on - strand, within flgCat 3893.813 kb on + strand, within flgCat 3893.814 kb on - strand, within flgCat 3893.831 kb on + strand, within flgCat 3893.873 kb on + strand, within flgCat 3893.874 kb on - strand, within flgCat 3894.001 kb on + strandat 3894.143 kb on - strand, within flgBat 3894.411 kb on - strandat 3894.436 kb on + strandat 3894.436 kb on + strandat 3894.474 kb on - strandat 3894.528 kb on + strandat 3894.602 kb on - strandat 3894.644 kb on - strandat 3894.647 kb on + strandat 3894.728 kb on + strand, within flgAat 3894.729 kb on - strand, within flgAat 3894.734 kb on + strand, within flgAat 3894.748 kb on + strand, within flgAat 3894.748 kb on + strand, within flgAat 3894.748 kb on + strand, within flgAat 3894.751 kb on + strand, within flgAat 3894.789 kb on - strand, within flgAat 3894.789 kb on - strand, within flgAat 3894.846 kb on + strand, within flgAat 3894.850 kb on + strand, within flgAat 3894.879 kb on + strand, within flgAat 3894.879 kb on + strand, within flgAat 3895.024 kb on + strand, within flgAat 3895.025 kb on - strand, within flgAat 3895.025 kb on - strand, within flgAat 3895.040 kb on + strand, within flgAat 3895.201 kb on + strandat 3895.233 kb on - strandat 3895.233 kb on - strandat 3895.239 kb on - strandat 3895.240 kb on + strandat 3895.249 kb on - strandat 3895.284 kb on + strandat 3895.284 kb on + strandat 3895.285 kb on - strandat 3895.285 kb on - strandat 3895.320 kb on + strandat 3895.331 kb on + strandat 3895.332 kb on - strandat 3895.523 kb on - strand, within flgMat 3895.549 kb on + strand, within flgMat 3895.614 kb on - strandat 3895.614 kb on - strandat 3895.623 kb on + strandat 3895.669 kb on - strand, within flgNat 3895.837 kb on - strand, within flgNat 3895.900 kb on - strand, within flgNat 3895.900 kb on - strand, within flgNat 3895.979 kb on + strand, within flgNat 3895.979 kb on + strand, within flgNat 3895.979 kb on + strand, within flgNat 3895.980 kb on - strand, within flgNat 3896.045 kb on + strandat 3896.081 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas61
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3,893,600 + +0.9
3,893,601 - +0.5
3,893,614 - +0.2
3,893,745 - flgC BNILDI_19360 0.33 -0.8
3,893,745 - flgC BNILDI_19360 0.33 +1.0
3,893,813 + flgC BNILDI_19360 0.50 -0.0
3,893,814 - flgC BNILDI_19360 0.50 +0.8
3,893,831 + flgC BNILDI_19360 0.54 +3.7
3,893,873 + flgC BNILDI_19360 0.64 +1.6
3,893,874 - flgC BNILDI_19360 0.65 +1.1
3,894,001 + -2.1
3,894,143 - flgB BNILDI_19365 0.29 +1.0
3,894,411 - +3.0
3,894,436 + +3.1
3,894,436 + +0.7
3,894,474 - -1.4
3,894,528 + +0.4
3,894,602 - +1.8
3,894,644 - -0.4
3,894,647 + -1.2
3,894,728 + flgA BNILDI_19370 0.21 +1.6
3,894,729 - flgA BNILDI_19370 0.21 -0.5
3,894,734 + flgA BNILDI_19370 0.22 +2.9
3,894,748 + flgA BNILDI_19370 0.24 +1.2
3,894,748 + flgA BNILDI_19370 0.24 +2.8
3,894,748 + flgA BNILDI_19370 0.24 +1.3
3,894,751 + flgA BNILDI_19370 0.24 +1.8
3,894,789 - flgA BNILDI_19370 0.30 +1.8
3,894,789 - flgA BNILDI_19370 0.30 -0.5
3,894,846 + flgA BNILDI_19370 0.39 +2.5
3,894,850 + flgA BNILDI_19370 0.39 -0.8
3,894,879 + flgA BNILDI_19370 0.44 +0.2
3,894,879 + flgA BNILDI_19370 0.44 +0.8
3,895,024 + flgA BNILDI_19370 0.66 +2.5
3,895,025 - flgA BNILDI_19370 0.66 -1.5
3,895,025 - flgA BNILDI_19370 0.66 +1.8
3,895,040 + flgA BNILDI_19370 0.68 -0.8
3,895,201 + +1.4
3,895,233 - -1.1
3,895,233 - +1.2
3,895,239 - +0.5
3,895,240 + -1.4
3,895,249 - +1.8
3,895,284 + -0.5
3,895,284 + -0.2
3,895,285 - +0.2
3,895,285 - -0.7
3,895,320 + +0.3
3,895,331 + -1.0
3,895,332 - -0.2
3,895,523 - flgM BNILDI_19375 0.67 +0.3
3,895,549 + flgM BNILDI_19375 0.76 -0.6
3,895,614 - +2.8
3,895,614 - +0.0
3,895,623 + +0.5
3,895,669 - flgN BNILDI_19380 0.11 -1.2
3,895,837 - flgN BNILDI_19380 0.51 +1.0
3,895,900 - flgN BNILDI_19380 0.66 +0.6
3,895,900 - flgN BNILDI_19380 0.66 +0.2
3,895,979 + flgN BNILDI_19380 0.85 +0.1
3,895,979 + flgN BNILDI_19380 0.85 -0.6
3,895,979 + flgN BNILDI_19380 0.85 -1.5
3,895,980 - flgN BNILDI_19380 0.85 +0.8
3,896,045 + +0.0
3,896,081 + -0.2

Or see this region's nucleotide sequence