Experiment: Bas61
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt oppA and BNILDI_18570 are separated by 373 nucleotides BNILDI_18570 and BNILDI_18575 overlap by 25 nucleotides BNILDI_18575 and ychE are separated by 26 nucleotides ychE and ychT are separated by 30 nucleotides
BNILDI_18565: oppA - oligopeptide ABC transporter substrate-binding protein OppA, at 3,737,980 to 3,739,656
oppA
BNILDI_18570: BNILDI_18570 - hypothetical protein, at 3,740,030 to 3,740,191
_18570
BNILDI_18575: BNILDI_18575 - hypothetical protein, at 3,740,167 to 3,740,322
_18575
BNILDI_18580: ychE - NAAT family transporter YchE, at 3,740,349 to 3,740,996
ychE
BNILDI_18585: ychT - Protein YchT, at 3,741,027 to 3,741,080
ychT
Position (kb)
3740
3741 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3739.248 kb on + strand, within oppA at 3739.249 kb on - strand, within oppA at 3739.249 kb on - strand, within oppA at 3739.249 kb on - strand, within oppA at 3739.250 kb on + strand, within oppA at 3739.250 kb on + strand, within oppA at 3739.251 kb on - strand, within oppA at 3739.335 kb on + strand, within oppA at 3739.393 kb on + strand, within oppA at 3739.422 kb on + strand, within oppA at 3739.483 kb on - strand, within oppA at 3739.526 kb on - strand at 3739.583 kb on - strand at 3739.586 kb on - strand at 3739.634 kb on + strand at 3739.634 kb on + strand at 3739.635 kb on - strand at 3739.635 kb on - strand at 3739.635 kb on - strand at 3739.711 kb on + strand at 3739.726 kb on + strand at 3739.726 kb on + strand at 3739.733 kb on - strand at 3739.744 kb on + strand at 3739.749 kb on - strand at 3739.774 kb on + strand at 3739.775 kb on - strand at 3740.093 kb on + strand, within BNILDI_18570 at 3740.300 kb on + strand, within BNILDI_18575 at 3740.351 kb on - strand at 3740.354 kb on + strand at 3740.354 kb on + strand at 3740.387 kb on + strand at 3740.394 kb on - strand at 3740.432 kb on + strand, within ychE at 3740.463 kb on + strand, within ychE at 3740.511 kb on + strand, within ychE at 3740.555 kb on + strand, within ychE at 3740.556 kb on - strand, within ychE at 3740.777 kb on + strand, within ychE at 3740.778 kb on - strand, within ychE at 3740.828 kb on + strand, within ychE at 3740.859 kb on - strand, within ychE at 3740.916 kb on - strand, within ychE at 3741.034 kb on + strand, within ychT at 3741.034 kb on + strand, within ychT at 3741.035 kb on - strand, within ychT at 3741.064 kb on - strand, within ychT at 3741.097 kb on - strand at 3741.097 kb on - strand at 3741.100 kb on + strand at 3741.109 kb on + strand at 3741.191 kb on - strand at 3741.283 kb on - strand at 3741.311 kb on + strand at 3741.312 kb on - strand at 3741.314 kb on + strand at 3741.314 kb on + strand at 3741.315 kb on - strand at 3741.320 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas61 remove 3,739,248 + oppA BNILDI_18565 0.76 +1.0 3,739,249 - oppA BNILDI_18565 0.76 -0.5 3,739,249 - oppA BNILDI_18565 0.76 +0.4 3,739,249 - oppA BNILDI_18565 0.76 -0.4 3,739,250 + oppA BNILDI_18565 0.76 +0.2 3,739,250 + oppA BNILDI_18565 0.76 +2.4 3,739,251 - oppA BNILDI_18565 0.76 +0.3 3,739,335 + oppA BNILDI_18565 0.81 +1.6 3,739,393 + oppA BNILDI_18565 0.84 +1.1 3,739,422 + oppA BNILDI_18565 0.86 +1.8 3,739,483 - oppA BNILDI_18565 0.90 -0.8 3,739,526 - +2.8 3,739,583 - +0.8 3,739,586 - +0.3 3,739,634 + +0.5 3,739,634 + -1.0 3,739,635 - -0.2 3,739,635 - +1.4 3,739,635 - -0.2 3,739,711 + -0.6 3,739,726 + -0.6 3,739,726 + -0.3 3,739,733 - +0.7 3,739,744 + +0.8 3,739,749 - -0.2 3,739,774 + -0.4 3,739,775 - -0.3 3,740,093 + BNILDI_18570 0.39 -0.1 3,740,300 + BNILDI_18575 0.85 +0.9 3,740,351 - +1.0 3,740,354 + -0.2 3,740,354 + +0.5 3,740,387 + +0.4 3,740,394 - +0.4 3,740,432 + ychE BNILDI_18580 0.13 +0.1 3,740,463 + ychE BNILDI_18580 0.18 +0.7 3,740,511 + ychE BNILDI_18580 0.25 +0.8 3,740,555 + ychE BNILDI_18580 0.32 -2.0 3,740,556 - ychE BNILDI_18580 0.32 -1.0 3,740,777 + ychE BNILDI_18580 0.66 -2.3 3,740,778 - ychE BNILDI_18580 0.66 -0.5 3,740,828 + ychE BNILDI_18580 0.74 +0.8 3,740,859 - ychE BNILDI_18580 0.79 +0.4 3,740,916 - ychE BNILDI_18580 0.88 -1.7 3,741,034 + ychT BNILDI_18585 0.13 +0.1 3,741,034 + ychT BNILDI_18585 0.13 -1.2 3,741,035 - ychT BNILDI_18585 0.15 +1.1 3,741,064 - ychT BNILDI_18585 0.69 -0.2 3,741,097 - +1.8 3,741,097 - +0.8 3,741,100 + -0.5 3,741,109 + -0.4 3,741,191 - +0.6 3,741,283 - +0.8 3,741,311 + -0.5 3,741,312 - -1.7 3,741,314 + +2.3 3,741,314 + -0.8 3,741,315 - +0.3 3,741,320 + +1.5
Or see this region's nucleotide sequence