Strain Fitness in Escherichia coli ECRC62 around BNILDI_18105

Experiment: Bas61

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntuspE and ynaJ are separated by 49 nucleotidesynaJ and ynaI are separated by 243 nucleotidesynaI and mppA are separated by 50 nucleotides BNILDI_18095: uspE - universal stress protein UspE, at 3,643,864 to 3,644,814 uspE BNILDI_18100: ynaJ - Uncharacterized protein YnaJ, at 3,644,864 to 3,645,121 ynaJ BNILDI_18105: ynaI - low conductance mechanosensitive channel YnaI, at 3,645,365 to 3,646,396 ynaI BNILDI_18110: mppA - murein tripeptide ABC transporter substrate-binding protein MppA, at 3,646,447 to 3,648,060 mppA Position (kb) 3645 3646 3647Strain fitness (log2 ratio) -2 -1 0 1 2at 3644.370 kb on + strand, within uspEat 3644.472 kb on + strand, within uspEat 3644.515 kb on - strand, within uspEat 3644.646 kb on - strand, within uspEat 3644.689 kb on - strand, within uspEat 3644.760 kb on + strandat 3644.760 kb on + strandat 3644.862 kb on - strandat 3644.866 kb on - strandat 3644.866 kb on - strandat 3644.890 kb on - strand, within ynaJat 3644.920 kb on + strand, within ynaJat 3645.000 kb on + strand, within ynaJat 3645.242 kb on - strandat 3645.258 kb on + strandat 3645.389 kb on - strandat 3645.548 kb on - strand, within ynaIat 3645.551 kb on + strand, within ynaIat 3645.558 kb on + strand, within ynaIat 3645.946 kb on + strand, within ynaIat 3645.946 kb on + strand, within ynaIat 3646.007 kb on + strand, within ynaIat 3646.194 kb on - strand, within ynaIat 3646.358 kb on + strandat 3646.358 kb on + strandat 3646.359 kb on - strandat 3646.394 kb on + strandat 3646.463 kb on + strandat 3646.464 kb on - strandat 3646.464 kb on - strandat 3646.467 kb on + strandat 3646.500 kb on + strandat 3646.501 kb on - strandat 3646.546 kb on - strandat 3646.548 kb on - strandat 3646.740 kb on + strand, within mppAat 3647.050 kb on - strand, within mppAat 3647.110 kb on + strand, within mppAat 3647.110 kb on + strand, within mppAat 3647.123 kb on - strand, within mppAat 3647.123 kb on - strand, within mppAat 3647.181 kb on - strand, within mppAat 3647.243 kb on + strand, within mppAat 3647.244 kb on - strand, within mppAat 3647.244 kb on - strand, within mppAat 3647.291 kb on + strand, within mppA

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas61
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3,644,370 + uspE BNILDI_18095 0.53 +1.1
3,644,472 + uspE BNILDI_18095 0.64 +0.6
3,644,515 - uspE BNILDI_18095 0.68 -0.3
3,644,646 - uspE BNILDI_18095 0.82 +0.2
3,644,689 - uspE BNILDI_18095 0.87 +1.8
3,644,760 + +0.6
3,644,760 + +1.5
3,644,862 - -2.0
3,644,866 - +1.8
3,644,866 - -1.9
3,644,890 - ynaJ BNILDI_18100 0.10 -0.0
3,644,920 + ynaJ BNILDI_18100 0.22 -0.4
3,645,000 + ynaJ BNILDI_18100 0.53 +0.5
3,645,242 - +0.9
3,645,258 + -0.8
3,645,389 - -0.4
3,645,548 - ynaI BNILDI_18105 0.18 -0.6
3,645,551 + ynaI BNILDI_18105 0.18 +0.8
3,645,558 + ynaI BNILDI_18105 0.19 -0.5
3,645,946 + ynaI BNILDI_18105 0.56 +0.4
3,645,946 + ynaI BNILDI_18105 0.56 +1.6
3,646,007 + ynaI BNILDI_18105 0.62 +1.1
3,646,194 - ynaI BNILDI_18105 0.80 +2.4
3,646,358 + -0.1
3,646,358 + +1.8
3,646,359 - +0.4
3,646,394 + -0.7
3,646,463 + +1.8
3,646,464 - +0.3
3,646,464 - +0.8
3,646,467 + -0.7
3,646,500 + -0.2
3,646,501 - +0.2
3,646,546 - +2.2
3,646,548 - -1.4
3,646,740 + mppA BNILDI_18110 0.18 -0.9
3,647,050 - mppA BNILDI_18110 0.37 +0.4
3,647,110 + mppA BNILDI_18110 0.41 +0.4
3,647,110 + mppA BNILDI_18110 0.41 -0.5
3,647,123 - mppA BNILDI_18110 0.42 +1.3
3,647,123 - mppA BNILDI_18110 0.42 -0.3
3,647,181 - mppA BNILDI_18110 0.45 +0.8
3,647,243 + mppA BNILDI_18110 0.49 +0.5
3,647,244 - mppA BNILDI_18110 0.49 -0.5
3,647,244 - mppA BNILDI_18110 0.49 -0.5
3,647,291 + mppA BNILDI_18110 0.52 -1.1

Or see this region's nucleotide sequence