Experiment: Bas61
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fumB and fumC are separated by 141 nucleotides fumC and tus overlap by 4 nucleotides
BNILDI_16940: fumB - class I fumarate hydratase, at 3,392,963 to 3,394,609
fumB
BNILDI_16945: fumC - class II fumarate hydratase, at 3,394,751 to 3,396,154
fumC
BNILDI_16950: tus - DNA replication terminus site-binding protein, at 3,396,151 to 3,397,080
tus
Position (kb)
3394
3395
3396
3397 Strain fitness (log2 ratio)
-1
0
1
2 at 3393.839 kb on + strand, within fumB at 3393.982 kb on + strand, within fumB at 3393.995 kb on + strand, within fumB at 3393.995 kb on + strand, within fumB at 3393.995 kb on + strand, within fumB at 3393.996 kb on - strand, within fumB at 3393.996 kb on - strand, within fumB at 3393.996 kb on - strand, within fumB at 3394.200 kb on - strand, within fumB at 3394.205 kb on - strand, within fumB at 3394.301 kb on + strand, within fumB at 3394.302 kb on - strand, within fumB at 3394.302 kb on - strand, within fumB at 3394.384 kb on + strand, within fumB at 3394.385 kb on - strand, within fumB at 3394.434 kb on + strand, within fumB at 3394.450 kb on + strand at 3394.450 kb on + strand at 3394.451 kb on - strand at 3394.637 kb on + strand at 3394.638 kb on - strand at 3394.756 kb on + strand at 3394.808 kb on - strand at 3394.935 kb on + strand, within fumC at 3395.057 kb on - strand, within fumC at 3395.415 kb on + strand, within fumC at 3395.510 kb on - strand, within fumC at 3395.900 kb on - strand, within fumC at 3396.086 kb on - strand at 3396.164 kb on + strand at 3396.164 kb on + strand at 3396.165 kb on - strand at 3396.435 kb on - strand, within tus at 3396.552 kb on + strand, within tus at 3396.553 kb on - strand, within tus at 3396.558 kb on + strand, within tus at 3396.559 kb on - strand, within tus at 3396.559 kb on - strand, within tus at 3396.559 kb on - strand, within tus at 3396.562 kb on - strand, within tus at 3396.577 kb on + strand, within tus at 3396.742 kb on - strand, within tus at 3396.782 kb on + strand, within tus at 3396.783 kb on - strand, within tus at 3396.851 kb on - strand, within tus at 3396.950 kb on - strand, within tus at 3397.044 kb on + strand at 3397.044 kb on + strand at 3397.061 kb on - strand at 3397.083 kb on + strand at 3397.083 kb on + strand at 3397.083 kb on + strand at 3397.084 kb on - strand at 3397.084 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas61 remove 3,393,839 + fumB BNILDI_16940 0.53 -0.1 3,393,982 + fumB BNILDI_16940 0.62 -0.2 3,393,995 + fumB BNILDI_16940 0.63 -0.4 3,393,995 + fumB BNILDI_16940 0.63 +1.5 3,393,995 + fumB BNILDI_16940 0.63 +0.8 3,393,996 - fumB BNILDI_16940 0.63 +0.6 3,393,996 - fumB BNILDI_16940 0.63 -1.5 3,393,996 - fumB BNILDI_16940 0.63 +1.0 3,394,200 - fumB BNILDI_16940 0.75 -0.2 3,394,205 - fumB BNILDI_16940 0.75 +1.7 3,394,301 + fumB BNILDI_16940 0.81 +0.3 3,394,302 - fumB BNILDI_16940 0.81 -0.3 3,394,302 - fumB BNILDI_16940 0.81 +0.7 3,394,384 + fumB BNILDI_16940 0.86 -0.2 3,394,385 - fumB BNILDI_16940 0.86 +1.0 3,394,434 + fumB BNILDI_16940 0.89 +0.6 3,394,450 + -0.5 3,394,450 + +0.1 3,394,451 - +1.0 3,394,637 + -0.3 3,394,638 - -0.6 3,394,756 + +1.1 3,394,808 - +0.5 3,394,935 + fumC BNILDI_16945 0.13 -0.1 3,395,057 - fumC BNILDI_16945 0.22 -0.6 3,395,415 + fumC BNILDI_16945 0.47 +1.0 3,395,510 - fumC BNILDI_16945 0.54 -0.6 3,395,900 - fumC BNILDI_16945 0.82 +0.7 3,396,086 - -0.6 3,396,164 + -1.0 3,396,164 + +0.7 3,396,165 - -0.2 3,396,435 - tus BNILDI_16950 0.31 +2.3 3,396,552 + tus BNILDI_16950 0.43 +1.1 3,396,553 - tus BNILDI_16950 0.43 -0.3 3,396,558 + tus BNILDI_16950 0.44 +0.1 3,396,559 - tus BNILDI_16950 0.44 -0.3 3,396,559 - tus BNILDI_16950 0.44 -1.3 3,396,559 - tus BNILDI_16950 0.44 +0.0 3,396,562 - tus BNILDI_16950 0.44 +0.7 3,396,577 + tus BNILDI_16950 0.46 -0.1 3,396,742 - tus BNILDI_16950 0.64 -0.9 3,396,782 + tus BNILDI_16950 0.68 -1.0 3,396,783 - tus BNILDI_16950 0.68 +1.0 3,396,851 - tus BNILDI_16950 0.75 +0.0 3,396,950 - tus BNILDI_16950 0.86 +1.3 3,397,044 + -0.3 3,397,044 + +1.1 3,397,061 - -0.1 3,397,083 + +0.1 3,397,083 + +0.1 3,397,083 + +0.1 3,397,084 - -0.0 3,397,084 - +0.0
Or see this region's nucleotide sequence