Experiment: Bas61
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt astA and astD overlap by 4 nucleotides astD and astB overlap by 4 nucleotides
BNILDI_16210: astA - arginine N-succinyltransferase, at 3,251,252 to 3,252,286
astA
BNILDI_16215: astD - succinylglutamate-semialdehyde dehydrogenase, at 3,252,283 to 3,253,761
astD
BNILDI_16220: astB - N-succinylarginine dihydrolase, at 3,253,758 to 3,255,101
astB
Position (kb)
3252
3253
3254 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3251.517 kb on - strand, within astA at 3251.663 kb on + strand, within astA at 3251.686 kb on + strand, within astA at 3251.858 kb on - strand, within astA at 3251.888 kb on + strand, within astA at 3251.888 kb on + strand, within astA at 3251.889 kb on - strand, within astA at 3251.934 kb on + strand, within astA at 3251.996 kb on + strand, within astA at 3252.365 kb on + strand at 3252.529 kb on - strand, within astD at 3252.714 kb on + strand, within astD at 3252.715 kb on - strand, within astD at 3252.745 kb on - strand, within astD at 3253.130 kb on + strand, within astD at 3253.335 kb on - strand, within astD at 3253.335 kb on - strand, within astD at 3253.473 kb on + strand, within astD at 3253.473 kb on + strand, within astD at 3253.810 kb on - strand at 3253.865 kb on + strand at 3253.894 kb on + strand, within astB at 3253.964 kb on + strand, within astB at 3254.160 kb on - strand, within astB at 3254.664 kb on + strand, within astB at 3254.664 kb on + strand, within astB
Per-strain Table
Position Strand Gene LocusTag Fraction Bas61 remove 3,251,517 - astA BNILDI_16210 0.26 -1.3 3,251,663 + astA BNILDI_16210 0.40 +0.4 3,251,686 + astA BNILDI_16210 0.42 +0.4 3,251,858 - astA BNILDI_16210 0.59 +1.1 3,251,888 + astA BNILDI_16210 0.61 -0.3 3,251,888 + astA BNILDI_16210 0.61 -0.6 3,251,889 - astA BNILDI_16210 0.62 -0.6 3,251,934 + astA BNILDI_16210 0.66 -0.4 3,251,996 + astA BNILDI_16210 0.72 -0.6 3,252,365 + -2.4 3,252,529 - astD BNILDI_16215 0.17 -0.8 3,252,714 + astD BNILDI_16215 0.29 +0.4 3,252,715 - astD BNILDI_16215 0.29 -0.6 3,252,745 - astD BNILDI_16215 0.31 +0.9 3,253,130 + astD BNILDI_16215 0.57 +0.6 3,253,335 - astD BNILDI_16215 0.71 +0.8 3,253,335 - astD BNILDI_16215 0.71 +0.5 3,253,473 + astD BNILDI_16215 0.80 +1.3 3,253,473 + astD BNILDI_16215 0.80 -1.0 3,253,810 - -0.3 3,253,865 + -1.3 3,253,894 + astB BNILDI_16220 0.10 +0.7 3,253,964 + astB BNILDI_16220 0.15 -1.3 3,254,160 - astB BNILDI_16220 0.30 +2.1 3,254,664 + astB BNILDI_16220 0.67 -0.6 3,254,664 + astB BNILDI_16220 0.67 +0.7
Or see this region's nucleotide sequence