Experiment: Bas61
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sdaA and nudL are separated by 183 nucleotides nudL and pabB are separated by 3 nucleotides pabB and yoaH are separated by 73 nucleotides yoaH and BNILDI_15875 are separated by 119 nucleotides
BNILDI_15855: sdaA - L-serine ammonia-lyase, at 3,181,744 to 3,183,108
sdaA
BNILDI_15860: nudL - CoA pyrophosphatase, at 3,183,292 to 3,183,870
nudL
BNILDI_15865: pabB - aminodeoxychorismate synthase component 1, at 3,183,874 to 3,185,235
pabB
BNILDI_15870: yoaH - YoaH family protein, at 3,185,309 to 3,185,488
yoaH
BNILDI_15875: BNILDI_15875 - DUF1889 domain-containing protein, at 3,185,608 to 3,185,907
_15875
Position (kb)
3183
3184
3185
3186 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3182.911 kb on - strand, within sdaA at 3183.102 kb on + strand at 3183.103 kb on - strand at 3183.115 kb on - strand at 3183.115 kb on - strand at 3183.151 kb on + strand at 3183.152 kb on - strand at 3183.152 kb on - strand at 3183.152 kb on - strand at 3183.164 kb on + strand at 3183.165 kb on - strand at 3183.165 kb on - strand at 3183.277 kb on + strand at 3183.284 kb on - strand at 3183.287 kb on + strand at 3183.287 kb on + strand at 3183.287 kb on + strand at 3183.288 kb on - strand at 3183.290 kb on - strand at 3183.411 kb on + strand, within nudL at 3183.412 kb on - strand, within nudL at 3183.412 kb on - strand, within nudL at 3183.415 kb on + strand, within nudL at 3183.416 kb on - strand, within nudL at 3183.416 kb on - strand, within nudL at 3183.416 kb on - strand, within nudL at 3183.440 kb on - strand, within nudL at 3183.516 kb on + strand, within nudL at 3183.550 kb on + strand, within nudL at 3183.550 kb on + strand, within nudL at 3183.550 kb on + strand, within nudL at 3183.703 kb on - strand, within nudL at 3183.790 kb on + strand, within nudL at 3183.876 kb on - strand at 3183.896 kb on - strand at 3183.896 kb on - strand at 3183.901 kb on - strand at 3183.903 kb on + strand at 3183.980 kb on - strand at 3183.980 kb on - strand at 3184.014 kb on + strand, within pabB at 3184.015 kb on - strand, within pabB at 3184.015 kb on - strand, within pabB at 3184.015 kb on - strand, within pabB at 3184.075 kb on - strand, within pabB at 3184.181 kb on - strand, within pabB at 3184.206 kb on + strand, within pabB at 3184.207 kb on - strand, within pabB at 3184.207 kb on - strand, within pabB at 3184.261 kb on + strand, within pabB at 3184.262 kb on - strand, within pabB at 3184.536 kb on + strand, within pabB at 3184.628 kb on + strand, within pabB at 3184.629 kb on - strand, within pabB at 3184.636 kb on + strand, within pabB at 3184.658 kb on + strand, within pabB at 3184.659 kb on - strand, within pabB at 3184.764 kb on + strand, within pabB at 3184.764 kb on + strand, within pabB at 3184.764 kb on + strand, within pabB at 3184.785 kb on + strand, within pabB at 3184.851 kb on + strand, within pabB at 3185.209 kb on - strand at 3185.305 kb on - strand at 3185.495 kb on - strand at 3185.516 kb on - strand at 3185.516 kb on - strand at 3185.561 kb on - strand at 3185.588 kb on + strand at 3185.637 kb on + strand at 3185.637 kb on + strand at 3185.638 kb on - strand, within BNILDI_15875 at 3185.638 kb on - strand, within BNILDI_15875 at 3185.639 kb on + strand, within BNILDI_15875 at 3185.639 kb on + strand, within BNILDI_15875 at 3185.640 kb on - strand, within BNILDI_15875 at 3185.640 kb on - strand, within BNILDI_15875 at 3185.797 kb on + strand, within BNILDI_15875 at 3185.806 kb on - strand, within BNILDI_15875 at 3185.857 kb on + strand, within BNILDI_15875 at 3185.857 kb on + strand, within BNILDI_15875 at 3185.889 kb on + strand at 3185.894 kb on - strand at 3185.894 kb on - strand at 3185.894 kb on - strand at 3185.896 kb on + strand at 3185.947 kb on - strand at 3185.948 kb on + strand at 3185.951 kb on - strand at 3185.951 kb on - strand at 3186.170 kb on - strand at 3186.173 kb on + strand at 3186.173 kb on + strand at 3186.176 kb on - strand at 3186.178 kb on - strand at 3186.181 kb on + strand at 3186.181 kb on + strand at 3186.182 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas61 remove 3,182,911 - sdaA BNILDI_15855 0.85 +0.5 3,183,102 + +0.2 3,183,103 - -0.3 3,183,115 - -0.1 3,183,115 - +0.4 3,183,151 + +0.2 3,183,152 - +0.3 3,183,152 - +0.5 3,183,152 - -0.3 3,183,164 + +1.0 3,183,165 - +0.8 3,183,165 - -0.6 3,183,277 + -0.1 3,183,284 - -0.3 3,183,287 + -0.6 3,183,287 + +1.2 3,183,287 + -0.9 3,183,288 - +0.3 3,183,290 - +1.0 3,183,411 + nudL BNILDI_15860 0.21 +0.6 3,183,412 - nudL BNILDI_15860 0.21 +0.3 3,183,412 - nudL BNILDI_15860 0.21 +0.1 3,183,415 + nudL BNILDI_15860 0.21 +0.3 3,183,416 - nudL BNILDI_15860 0.21 +0.7 3,183,416 - nudL BNILDI_15860 0.21 -1.1 3,183,416 - nudL BNILDI_15860 0.21 +0.3 3,183,440 - nudL BNILDI_15860 0.26 -0.4 3,183,516 + nudL BNILDI_15860 0.39 -1.7 3,183,550 + nudL BNILDI_15860 0.45 -1.0 3,183,550 + nudL BNILDI_15860 0.45 +1.7 3,183,550 + nudL BNILDI_15860 0.45 -0.6 3,183,703 - nudL BNILDI_15860 0.71 -1.1 3,183,790 + nudL BNILDI_15860 0.86 -0.6 3,183,876 - +0.7 3,183,896 - +0.7 3,183,896 - -1.0 3,183,901 - -0.5 3,183,903 + -0.5 3,183,980 - -0.6 3,183,980 - -0.3 3,184,014 + pabB BNILDI_15865 0.10 -0.1 3,184,015 - pabB BNILDI_15865 0.10 -1.3 3,184,015 - pabB BNILDI_15865 0.10 -1.6 3,184,015 - pabB BNILDI_15865 0.10 -0.1 3,184,075 - pabB BNILDI_15865 0.15 -1.3 3,184,181 - pabB BNILDI_15865 0.23 +0.1 3,184,206 + pabB BNILDI_15865 0.24 -0.8 3,184,207 - pabB BNILDI_15865 0.24 +1.7 3,184,207 - pabB BNILDI_15865 0.24 -0.3 3,184,261 + pabB BNILDI_15865 0.28 -0.2 3,184,262 - pabB BNILDI_15865 0.28 -0.3 3,184,536 + pabB BNILDI_15865 0.49 -0.3 3,184,628 + pabB BNILDI_15865 0.55 -0.5 3,184,629 - pabB BNILDI_15865 0.55 -1.1 3,184,636 + pabB BNILDI_15865 0.56 -0.8 3,184,658 + pabB BNILDI_15865 0.58 -0.6 3,184,659 - pabB BNILDI_15865 0.58 +0.5 3,184,764 + pabB BNILDI_15865 0.65 +0.2 3,184,764 + pabB BNILDI_15865 0.65 +1.2 3,184,764 + pabB BNILDI_15865 0.65 +0.2 3,184,785 + pabB BNILDI_15865 0.67 -1.3 3,184,851 + pabB BNILDI_15865 0.72 +0.7 3,185,209 - +0.7 3,185,305 - +0.1 3,185,495 - +0.7 3,185,516 - -0.3 3,185,516 - +1.3 3,185,561 - +0.7 3,185,588 + +0.9 3,185,637 + -0.2 3,185,637 + +0.0 3,185,638 - BNILDI_15875 0.10 -0.2 3,185,638 - BNILDI_15875 0.10 -0.4 3,185,639 + BNILDI_15875 0.10 -0.4 3,185,639 + BNILDI_15875 0.10 -1.3 3,185,640 - BNILDI_15875 0.11 -1.0 3,185,640 - BNILDI_15875 0.11 -0.3 3,185,797 + BNILDI_15875 0.63 -0.6 3,185,806 - BNILDI_15875 0.66 -1.3 3,185,857 + BNILDI_15875 0.83 -0.5 3,185,857 + BNILDI_15875 0.83 +0.2 3,185,889 + +0.2 3,185,894 - -0.5 3,185,894 - +1.0 3,185,894 - +0.2 3,185,896 + -0.1 3,185,947 - +3.1 3,185,948 + +0.0 3,185,951 - +0.7 3,185,951 - +0.2 3,186,170 - +0.5 3,186,173 + +1.3 3,186,173 + -0.7 3,186,176 - +0.1 3,186,178 - +0.2 3,186,181 + -0.8 3,186,181 + -0.1 3,186,182 - -0.8
Or see this region's nucleotide sequence