Experiment: Bas61
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt manZ and manY are separated by 12 nucleotides manY and manX are separated by 62 nucleotides manX and BNILDI_15840 are separated by 265 nucleotides BNILDI_15840 and yoaE overlap by 13 nucleotides
BNILDI_15825: manZ - PTS mannose transporter subunit IID, at 3,175,294 to 3,176,145
manZ
BNILDI_15830: manY - PTS mannose transporter subunit IIC, at 3,176,158 to 3,176,958
manY
BNILDI_15835: manX - PTS mannose transporter subunit IIAB, at 3,177,021 to 3,177,992
manX
BNILDI_15840: BNILDI_15840 - Cytoplasmic protein, at 3,178,258 to 3,178,467
_15840
BNILDI_15845: yoaE - CNNM family cation transport protein YoaE, at 3,178,455 to 3,180,011
yoaE
Position (kb)
3177
3178 Strain fitness (log2 ratio)
-2
-1
0
1 at 3176.037 kb on + strand, within manZ at 3176.037 kb on + strand, within manZ at 3176.037 kb on + strand, within manZ at 3176.070 kb on + strand at 3176.136 kb on + strand at 3176.378 kb on + strand, within manY at 3176.459 kb on - strand, within manY at 3176.715 kb on + strand, within manY at 3176.725 kb on - strand, within manY at 3176.809 kb on - strand, within manY at 3176.850 kb on + strand, within manY at 3176.851 kb on - strand, within manY at 3176.926 kb on + strand at 3176.926 kb on + strand at 3176.961 kb on + strand at 3176.978 kb on + strand at 3177.022 kb on + strand at 3177.243 kb on + strand, within manX at 3177.260 kb on + strand, within manX at 3177.261 kb on - strand, within manX at 3177.290 kb on - strand, within manX at 3177.304 kb on + strand, within manX at 3177.333 kb on + strand, within manX at 3177.333 kb on + strand, within manX at 3177.334 kb on - strand, within manX at 3177.389 kb on + strand, within manX at 3177.791 kb on + strand, within manX at 3177.816 kb on - strand, within manX at 3177.973 kb on - strand at 3178.007 kb on - strand at 3178.412 kb on + strand, within BNILDI_15840 at 3178.412 kb on + strand, within BNILDI_15840 at 3178.428 kb on + strand, within BNILDI_15840 at 3178.432 kb on + strand, within BNILDI_15840 at 3178.721 kb on + strand, within yoaE at 3178.721 kb on + strand, within yoaE at 3178.721 kb on + strand, within yoaE
Per-strain Table
Position Strand Gene LocusTag Fraction Bas61 remove 3,176,037 + manZ BNILDI_15825 0.87 +0.1 3,176,037 + manZ BNILDI_15825 0.87 -1.4 3,176,037 + manZ BNILDI_15825 0.87 +0.2 3,176,070 + +0.7 3,176,136 + +1.5 3,176,378 + manY BNILDI_15830 0.27 +1.1 3,176,459 - manY BNILDI_15830 0.38 -0.2 3,176,715 + manY BNILDI_15830 0.70 +0.2 3,176,725 - manY BNILDI_15830 0.71 -0.5 3,176,809 - manY BNILDI_15830 0.81 -1.2 3,176,850 + manY BNILDI_15830 0.86 -0.6 3,176,851 - manY BNILDI_15830 0.87 -0.1 3,176,926 + +0.4 3,176,926 + +1.4 3,176,961 + -1.2 3,176,978 + +0.8 3,177,022 + -1.1 3,177,243 + manX BNILDI_15835 0.23 +1.3 3,177,260 + manX BNILDI_15835 0.25 -0.0 3,177,261 - manX BNILDI_15835 0.25 -0.0 3,177,290 - manX BNILDI_15835 0.28 +0.2 3,177,304 + manX BNILDI_15835 0.29 +0.5 3,177,333 + manX BNILDI_15835 0.32 -0.7 3,177,333 + manX BNILDI_15835 0.32 +0.7 3,177,334 - manX BNILDI_15835 0.32 +0.6 3,177,389 + manX BNILDI_15835 0.38 +0.6 3,177,791 + manX BNILDI_15835 0.79 -0.0 3,177,816 - manX BNILDI_15835 0.82 +0.2 3,177,973 - -1.2 3,178,007 - +0.1 3,178,412 + BNILDI_15840 0.73 +0.5 3,178,412 + BNILDI_15840 0.73 -2.1 3,178,428 + BNILDI_15840 0.81 -0.6 3,178,432 + BNILDI_15840 0.83 -0.0 3,178,721 + yoaE BNILDI_15845 0.17 +0.6 3,178,721 + yoaE BNILDI_15845 0.17 -0.3 3,178,721 + yoaE BNILDI_15845 0.17 +0.4
Or see this region's nucleotide sequence