Strain Fitness in Escherichia coli ECRC62 around BNILDI_15650

Experiment: Bas61

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntedd and kdgA are separated by 36 nucleotideskdgA and purT are separated by 55 nucleotides BNILDI_15645: edd - phosphogluconate dehydratase, at 3,145,436 to 3,147,247 edd BNILDI_15650: kdgA - bifunctional 4-hydroxy-2-oxoglutarate aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase, at 3,147,284 to 3,147,925 kdgA BNILDI_15655: purT - formate-dependent phosphoribosylglycinamide formyltransferase, at 3,147,981 to 3,149,159 purT Position (kb) 3147 3148Strain fitness (log2 ratio) -2 -1 0 1at 3146.577 kb on - strand, within eddat 3146.895 kb on + strand, within eddat 3146.895 kb on + strand, within eddat 3146.899 kb on + strand, within eddat 3146.987 kb on - strand, within eddat 3147.607 kb on + strand, within kdgAat 3147.607 kb on + strand, within kdgAat 3147.615 kb on + strand, within kdgAat 3147.773 kb on - strand, within kdgAat 3147.773 kb on - strand, within kdgAat 3147.773 kb on - strand, within kdgAat 3147.788 kb on + strand, within kdgAat 3147.789 kb on - strand, within kdgAat 3147.797 kb on + strand, within kdgAat 3147.886 kb on + strandat 3147.906 kb on + strandat 3147.906 kb on + strandat 3147.944 kb on + strandat 3147.944 kb on + strandat 3147.983 kb on - strandat 3148.249 kb on - strand, within purTat 3148.249 kb on - strand, within purTat 3148.249 kb on - strand, within purTat 3148.249 kb on - strand, within purTat 3148.249 kb on - strand, within purTat 3148.498 kb on - strand, within purTat 3148.658 kb on + strand, within purTat 3148.905 kb on - strand, within purT

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas61
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3,146,577 - edd BNILDI_15645 0.63 -0.3
3,146,895 + edd BNILDI_15645 0.81 -0.4
3,146,895 + edd BNILDI_15645 0.81 +0.7
3,146,899 + edd BNILDI_15645 0.81 +0.7
3,146,987 - edd BNILDI_15645 0.86 -0.7
3,147,607 + kdgA BNILDI_15650 0.50 -1.1
3,147,607 + kdgA BNILDI_15650 0.50 +0.7
3,147,615 + kdgA BNILDI_15650 0.52 -2.7
3,147,773 - kdgA BNILDI_15650 0.76 -1.0
3,147,773 - kdgA BNILDI_15650 0.76 -0.1
3,147,773 - kdgA BNILDI_15650 0.76 -1.2
3,147,788 + kdgA BNILDI_15650 0.79 +0.4
3,147,789 - kdgA BNILDI_15650 0.79 +0.1
3,147,797 + kdgA BNILDI_15650 0.80 -0.3
3,147,886 + -0.9
3,147,906 + -0.6
3,147,906 + -1.1
3,147,944 + -0.1
3,147,944 + +1.7
3,147,983 - +0.5
3,148,249 - purT BNILDI_15655 0.23 +0.3
3,148,249 - purT BNILDI_15655 0.23 +0.4
3,148,249 - purT BNILDI_15655 0.23 +1.7
3,148,249 - purT BNILDI_15655 0.23 -0.9
3,148,249 - purT BNILDI_15655 0.23 -0.1
3,148,498 - purT BNILDI_15655 0.44 +1.5
3,148,658 + purT BNILDI_15655 0.57 -1.4
3,148,905 - purT BNILDI_15655 0.78 +0.0

Or see this region's nucleotide sequence