Experiment: Bas61
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fliM and fliL are separated by 4 nucleotides fliL and fliK are separated by 104 nucleotides fliK and fliJ overlap by 4 nucleotides fliJ and fliI are separated by 18 nucleotides
BNILDI_15100: fliM - flagellar motor switch protein FliM, at 3,043,606 to 3,044,610
fliM
BNILDI_15105: fliL - flagellar basal body-associated protein FliL, at 3,044,615 to 3,045,079
fliL
BNILDI_15110: fliK - flagellar hook length control protein FliK, at 3,045,184 to 3,046,311
fliK
BNILDI_15115: fliJ - flagella biosynthesis chaperone FliJ, at 3,046,308 to 3,046,751
fliJ
BNILDI_15120: fliI - flagellum-specific ATP synthase FliI, at 3,046,770 to 3,048,143
fliI
Position (kb)
3045
3046
3047 Strain fitness (log2 ratio)
-1
0
1
2 at 3044.375 kb on - strand, within fliM at 3044.599 kb on - strand at 3044.637 kb on + strand at 3044.803 kb on - strand, within fliL at 3044.859 kb on + strand, within fliL at 3044.910 kb on + strand, within fliL at 3044.980 kb on - strand, within fliL at 3045.165 kb on + strand at 3045.173 kb on + strand at 3045.229 kb on + strand at 3045.230 kb on - strand at 3045.230 kb on - strand at 3045.230 kb on - strand at 3045.232 kb on - strand at 3045.253 kb on - strand at 3045.336 kb on + strand, within fliK at 3045.396 kb on + strand, within fliK at 3045.434 kb on - strand, within fliK at 3045.475 kb on - strand, within fliK at 3045.508 kb on + strand, within fliK at 3045.571 kb on - strand, within fliK at 3045.581 kb on - strand, within fliK at 3045.666 kb on + strand, within fliK at 3045.670 kb on + strand, within fliK at 3045.740 kb on - strand, within fliK at 3046.147 kb on - strand, within fliK at 3046.291 kb on + strand at 3046.584 kb on - strand, within fliJ at 3046.618 kb on + strand, within fliJ at 3046.771 kb on + strand at 3046.852 kb on + strand at 3046.915 kb on + strand, within fliI
Per-strain Table
Position Strand Gene LocusTag Fraction Bas61 remove 3,044,375 - fliM BNILDI_15100 0.77 +0.5 3,044,599 - +0.3 3,044,637 + +2.3 3,044,803 - fliL BNILDI_15105 0.40 +0.4 3,044,859 + fliL BNILDI_15105 0.52 -0.6 3,044,910 + fliL BNILDI_15105 0.63 -0.2 3,044,980 - fliL BNILDI_15105 0.78 -0.0 3,045,165 + +2.7 3,045,173 + +0.0 3,045,229 + +0.5 3,045,230 - +2.1 3,045,230 - -0.0 3,045,230 - -0.0 3,045,232 - -0.6 3,045,253 - -0.2 3,045,336 + fliK BNILDI_15110 0.13 +0.3 3,045,396 + fliK BNILDI_15110 0.19 +1.6 3,045,434 - fliK BNILDI_15110 0.22 +0.6 3,045,475 - fliK BNILDI_15110 0.26 +0.8 3,045,508 + fliK BNILDI_15110 0.29 +0.8 3,045,571 - fliK BNILDI_15110 0.34 +0.9 3,045,581 - fliK BNILDI_15110 0.35 +0.2 3,045,666 + fliK BNILDI_15110 0.43 +2.3 3,045,670 + fliK BNILDI_15110 0.43 -0.1 3,045,740 - fliK BNILDI_15110 0.49 -0.7 3,046,147 - fliK BNILDI_15110 0.85 +1.7 3,046,291 + -1.6 3,046,584 - fliJ BNILDI_15115 0.62 +1.5 3,046,618 + fliJ BNILDI_15115 0.70 +1.3 3,046,771 + +2.0 3,046,852 + +1.6 3,046,915 + fliI BNILDI_15120 0.11 +0.9
Or see this region's nucleotide sequence