Strain Fitness in Escherichia coli ECRC62 around BNILDI_13300

Experiment: Bas61

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrpnB and yfcH are separated by 47 nucleotidesyfcH and folX are separated by 20 nucleotidesfolX and yfcG are separated by 56 nucleotidesyfcG and yfcF are separated by 135 nucleotides BNILDI_13290: rpnB - recombination-promoting nuclease RpnB, at 2,661,526 to 2,662,416 rpnB BNILDI_13295: yfcH - Epimerase family protein YfcH, at 2,662,464 to 2,663,357 yfcH BNILDI_13300: folX - dihydroneopterin triphosphate 2'-epimerase, at 2,663,378 to 2,663,740 folX BNILDI_13305: yfcG - GSH-dependent disulfide bond oxidoreductase, at 2,663,797 to 2,664,444 yfcG BNILDI_13310: yfcF - glutathione transferase, at 2,664,580 to 2,665,224 yfcF Position (kb) 2663 2664Strain fitness (log2 ratio) -2 -1 0 1 2at 2662.415 kb on - strandat 2662.422 kb on + strandat 2662.423 kb on - strandat 2662.423 kb on - strandat 2662.456 kb on - strandat 2662.563 kb on + strand, within yfcHat 2662.563 kb on + strand, within yfcHat 2662.587 kb on + strand, within yfcHat 2662.697 kb on + strand, within yfcHat 2662.996 kb on - strand, within yfcHat 2662.996 kb on - strand, within yfcHat 2662.996 kb on - strand, within yfcHat 2663.009 kb on - strand, within yfcHat 2663.326 kb on - strandat 2663.350 kb on - strandat 2663.457 kb on + strand, within folXat 2663.549 kb on - strand, within folXat 2663.641 kb on + strand, within folXat 2663.693 kb on - strand, within folXat 2663.711 kb on + strandat 2663.760 kb on + strandat 2663.761 kb on - strandat 2663.765 kb on - strandat 2663.765 kb on - strandat 2663.765 kb on - strandat 2663.774 kb on + strandat 2663.775 kb on - strandat 2663.798 kb on + strandat 2663.799 kb on - strandat 2663.897 kb on + strand, within yfcGat 2663.898 kb on - strand, within yfcGat 2663.898 kb on - strand, within yfcGat 2663.935 kb on - strand, within yfcGat 2663.969 kb on + strand, within yfcGat 2664.131 kb on - strand, within yfcGat 2664.212 kb on + strand, within yfcGat 2664.352 kb on + strand, within yfcGat 2664.431 kb on + strandat 2664.461 kb on + strandat 2664.462 kb on - strandat 2664.482 kb on + strandat 2664.510 kb on - strandat 2664.541 kb on - strandat 2664.542 kb on + strandat 2664.543 kb on - strandat 2664.543 kb on - strandat 2664.579 kb on - strandat 2664.631 kb on + strandat 2664.631 kb on + strandat 2664.632 kb on - strandat 2664.632 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas61
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2,662,415 - +1.1
2,662,422 + +0.1
2,662,423 - +1.2
2,662,423 - -1.0
2,662,456 - +0.5
2,662,563 + yfcH BNILDI_13295 0.11 +0.7
2,662,563 + yfcH BNILDI_13295 0.11 +0.2
2,662,587 + yfcH BNILDI_13295 0.14 -0.5
2,662,697 + yfcH BNILDI_13295 0.26 +0.5
2,662,996 - yfcH BNILDI_13295 0.60 -0.6
2,662,996 - yfcH BNILDI_13295 0.60 +0.7
2,662,996 - yfcH BNILDI_13295 0.60 +1.1
2,663,009 - yfcH BNILDI_13295 0.61 -0.8
2,663,326 - -2.0
2,663,350 - +0.2
2,663,457 + folX BNILDI_13300 0.22 -0.2
2,663,549 - folX BNILDI_13300 0.47 +1.5
2,663,641 + folX BNILDI_13300 0.72 +0.5
2,663,693 - folX BNILDI_13300 0.87 +0.8
2,663,711 + -1.6
2,663,760 + -0.0
2,663,761 - -0.5
2,663,765 - +1.3
2,663,765 - +1.1
2,663,765 - -0.2
2,663,774 + +0.8
2,663,775 - +0.7
2,663,798 + +1.8
2,663,799 - -0.9
2,663,897 + yfcG BNILDI_13305 0.15 +0.7
2,663,898 - yfcG BNILDI_13305 0.16 -0.1
2,663,898 - yfcG BNILDI_13305 0.16 +0.4
2,663,935 - yfcG BNILDI_13305 0.21 +1.0
2,663,969 + yfcG BNILDI_13305 0.27 -0.2
2,664,131 - yfcG BNILDI_13305 0.52 -1.3
2,664,212 + yfcG BNILDI_13305 0.64 -0.4
2,664,352 + yfcG BNILDI_13305 0.86 +0.4
2,664,431 + +0.8
2,664,461 + -0.8
2,664,462 - -0.2
2,664,482 + -0.2
2,664,510 - +0.2
2,664,541 - -0.5
2,664,542 + -0.4
2,664,543 - +0.2
2,664,543 - +0.1
2,664,579 - -0.5
2,664,631 + +0.0
2,664,631 + -0.4
2,664,632 - -0.4
2,664,632 - +0.9

Or see this region's nucleotide sequence