Experiment: Bas61
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gutM and srlD are separated by 104 nucleotides srlD and srlB are separated by 4 nucleotides srlB and srlE are separated by 10 nucleotides srlE and srlA overlap by 4 nucleotides
BNILDI_11070: gutM - transcriptional regulator GutM, at 2,238,412 to 2,238,771
gutM
BNILDI_11075: srlD - sorbitol-6-phosphate dehydrogenase, at 2,238,876 to 2,239,655
srlD
BNILDI_11080: srlB - PTS glucitol/sorbitol transporter subunit IIA, at 2,239,660 to 2,240,031
srlB
BNILDI_11085: srlE - PTS glucitol/sorbitol transporter subunit IIB, at 2,240,042 to 2,241,001
srlE
BNILDI_11090: srlA - PTS glucitol/sorbitol transporter subunit IIC, at 2,240,998 to 2,241,561
srlA
Position (kb)
2239
2240
2241 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2238.670 kb on + strand, within gutM at 2238.670 kb on + strand, within gutM at 2238.670 kb on + strand, within gutM at 2238.671 kb on - strand, within gutM at 2238.671 kb on - strand, within gutM at 2238.671 kb on - strand, within gutM at 2238.690 kb on + strand, within gutM at 2238.772 kb on - strand at 2238.788 kb on - strand at 2238.805 kb on - strand at 2238.836 kb on + strand at 2238.837 kb on - strand at 2238.839 kb on - strand at 2238.925 kb on + strand at 2238.959 kb on + strand, within srlD at 2238.960 kb on - strand, within srlD at 2239.012 kb on + strand, within srlD at 2239.012 kb on + strand, within srlD at 2239.012 kb on + strand, within srlD at 2239.013 kb on - strand, within srlD at 2239.016 kb on - strand, within srlD at 2239.310 kb on - strand, within srlD at 2239.330 kb on - strand, within srlD at 2239.383 kb on + strand, within srlD at 2239.432 kb on - strand, within srlD at 2239.508 kb on - strand, within srlD at 2239.858 kb on - strand, within srlB at 2240.023 kb on - strand at 2240.044 kb on - strand at 2240.100 kb on - strand at 2240.225 kb on + strand, within srlE at 2240.226 kb on - strand, within srlE at 2240.428 kb on + strand, within srlE at 2240.484 kb on - strand, within srlE at 2240.602 kb on - strand, within srlE at 2240.679 kb on + strand, within srlE at 2240.679 kb on + strand, within srlE at 2240.735 kb on - strand, within srlE at 2240.737 kb on + strand, within srlE at 2240.738 kb on - strand, within srlE at 2240.866 kb on + strand, within srlE at 2240.916 kb on + strand at 2240.917 kb on - strand at 2240.917 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas61 remove 2,238,670 + gutM BNILDI_11070 0.72 +0.1 2,238,670 + gutM BNILDI_11070 0.72 +0.4 2,238,670 + gutM BNILDI_11070 0.72 +0.2 2,238,671 - gutM BNILDI_11070 0.72 -0.0 2,238,671 - gutM BNILDI_11070 0.72 +1.2 2,238,671 - gutM BNILDI_11070 0.72 -0.4 2,238,690 + gutM BNILDI_11070 0.77 -1.6 2,238,772 - -1.5 2,238,788 - +0.7 2,238,805 - -0.8 2,238,836 + -0.8 2,238,837 - +0.5 2,238,839 - -0.3 2,238,925 + +0.6 2,238,959 + srlD BNILDI_11075 0.11 +0.9 2,238,960 - srlD BNILDI_11075 0.11 -0.9 2,239,012 + srlD BNILDI_11075 0.17 -0.2 2,239,012 + srlD BNILDI_11075 0.17 -1.2 2,239,012 + srlD BNILDI_11075 0.17 +0.7 2,239,013 - srlD BNILDI_11075 0.18 -0.2 2,239,016 - srlD BNILDI_11075 0.18 +0.8 2,239,310 - srlD BNILDI_11075 0.56 +0.2 2,239,330 - srlD BNILDI_11075 0.58 -1.0 2,239,383 + srlD BNILDI_11075 0.65 +1.3 2,239,432 - srlD BNILDI_11075 0.71 -0.8 2,239,508 - srlD BNILDI_11075 0.81 +2.8 2,239,858 - srlB BNILDI_11080 0.53 +0.0 2,240,023 - +0.7 2,240,044 - +0.0 2,240,100 - +0.0 2,240,225 + srlE BNILDI_11085 0.19 +0.2 2,240,226 - srlE BNILDI_11085 0.19 -0.2 2,240,428 + srlE BNILDI_11085 0.40 +0.0 2,240,484 - srlE BNILDI_11085 0.46 +1.1 2,240,602 - srlE BNILDI_11085 0.58 +1.8 2,240,679 + srlE BNILDI_11085 0.66 -0.4 2,240,679 + srlE BNILDI_11085 0.66 -1.9 2,240,735 - srlE BNILDI_11085 0.72 +0.0 2,240,737 + srlE BNILDI_11085 0.72 +0.1 2,240,738 - srlE BNILDI_11085 0.72 +0.4 2,240,866 + srlE BNILDI_11085 0.86 -1.6 2,240,916 + -0.3 2,240,917 - +1.2 2,240,917 - -0.0
Or see this region's nucleotide sequence