Strain Fitness in Escherichia coli ECRC62 around BNILDI_08600

Experiment: Bas61

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrsmH and BNILDI_08595 overlap by 4 nucleotidesBNILDI_08595 and ftsI are separated by 15 nucleotidesftsI and murE overlap by 14 nucleotides BNILDI_08590: rsmH - 16S rRNA (cytosine(1402)-N(4))-methyltransferase RsmH, at 1,719,302 to 1,720,243 rsmH BNILDI_08595: BNILDI_08595 - penicillin-binding protein PBP3 FtsI, at 1,720,240 to 1,720,605 _08595 BNILDI_08600: ftsI - peptidoglycan glycosyltransferase FtsI, at 1,720,621 to 1,722,387 ftsI BNILDI_08605: murE - UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-di aminopimelate ligase, at 1,722,374 to 1,723,861 murE Position (kb) 1720 1721 1722 1723Strain fitness (log2 ratio) -1 0 1at 1719.682 kb on + strand, within rsmHat 1719.886 kb on + strand, within rsmHat 1720.057 kb on + strand, within rsmHat 1720.057 kb on + strand, within rsmHat 1720.603 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas61
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1,719,682 + rsmH BNILDI_08590 0.40 +0.3
1,719,886 + rsmH BNILDI_08590 0.62 -0.1
1,720,057 + rsmH BNILDI_08590 0.80 -0.5
1,720,057 + rsmH BNILDI_08590 0.80 -0.2
1,720,603 + +0.8

Or see this region's nucleotide sequence