Experiment: Bas61
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt purA and nsrR are separated by 204 nucleotides nsrR and rnr are separated by 38 nucleotides
BNILDI_06995: purA - adenylosuccinate synthase, at 1,399,430 to 1,400,728
purA
BNILDI_07000: nsrR - nitric oxide-sensing transcriptional repressor NsrR, at 1,400,933 to 1,401,358
nsrR
BNILDI_07005: rnr - ribonuclease R, at 1,401,397 to 1,403,838
rnr
Position (kb)
1400
1401
1402 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1399.958 kb on + strand, within purA at 1399.999 kb on + strand, within purA at 1400.029 kb on + strand, within purA at 1400.131 kb on + strand, within purA at 1400.135 kb on + strand, within purA at 1400.146 kb on - strand, within purA at 1400.350 kb on + strand, within purA at 1400.727 kb on - strand at 1400.736 kb on - strand at 1400.748 kb on + strand at 1400.776 kb on + strand at 1400.885 kb on - strand at 1400.895 kb on + strand at 1400.901 kb on + strand at 1400.941 kb on - strand at 1400.957 kb on + strand at 1400.978 kb on + strand, within nsrR at 1400.978 kb on + strand, within nsrR at 1400.979 kb on - strand, within nsrR at 1400.979 kb on - strand, within nsrR at 1401.014 kb on - strand, within nsrR at 1401.014 kb on - strand, within nsrR at 1401.079 kb on + strand, within nsrR at 1401.080 kb on - strand, within nsrR at 1401.080 kb on - strand, within nsrR at 1401.136 kb on + strand, within nsrR at 1401.157 kb on + strand, within nsrR at 1401.157 kb on + strand, within nsrR at 1401.157 kb on + strand, within nsrR at 1401.158 kb on - strand, within nsrR at 1401.226 kb on + strand, within nsrR at 1401.296 kb on + strand, within nsrR at 1401.335 kb on + strand at 1401.337 kb on + strand at 1401.337 kb on + strand at 1401.342 kb on + strand at 1401.453 kb on + strand at 1401.708 kb on + strand, within rnr at 1401.816 kb on + strand, within rnr at 1401.862 kb on + strand, within rnr at 1401.922 kb on - strand, within rnr at 1401.927 kb on - strand, within rnr at 1402.100 kb on - strand, within rnr at 1402.307 kb on - strand, within rnr at 1402.309 kb on + strand, within rnr at 1402.309 kb on + strand, within rnr at 1402.309 kb on + strand, within rnr at 1402.309 kb on + strand, within rnr at 1402.310 kb on - strand, within rnr at 1402.310 kb on - strand, within rnr at 1402.310 kb on - strand, within rnr at 1402.310 kb on - strand, within rnr at 1402.310 kb on - strand, within rnr
Per-strain Table
Position Strand Gene LocusTag Fraction Bas61 remove 1,399,958 + purA BNILDI_06995 0.41 +0.9 1,399,999 + purA BNILDI_06995 0.44 +0.9 1,400,029 + purA BNILDI_06995 0.46 -1.4 1,400,131 + purA BNILDI_06995 0.54 -0.0 1,400,135 + purA BNILDI_06995 0.54 +0.9 1,400,146 - purA BNILDI_06995 0.55 -1.2 1,400,350 + purA BNILDI_06995 0.71 -0.1 1,400,727 - -0.8 1,400,736 - +1.6 1,400,748 + -0.3 1,400,776 + +0.8 1,400,885 - +0.3 1,400,895 + +0.3 1,400,901 + +0.2 1,400,941 - +0.8 1,400,957 + -0.7 1,400,978 + nsrR BNILDI_07000 0.11 -1.2 1,400,978 + nsrR BNILDI_07000 0.11 -1.0 1,400,979 - nsrR BNILDI_07000 0.11 -0.6 1,400,979 - nsrR BNILDI_07000 0.11 -1.0 1,401,014 - nsrR BNILDI_07000 0.19 +0.3 1,401,014 - nsrR BNILDI_07000 0.19 -1.9 1,401,079 + nsrR BNILDI_07000 0.34 -0.1 1,401,080 - nsrR BNILDI_07000 0.35 -0.7 1,401,080 - nsrR BNILDI_07000 0.35 -0.1 1,401,136 + nsrR BNILDI_07000 0.48 +0.9 1,401,157 + nsrR BNILDI_07000 0.53 +0.3 1,401,157 + nsrR BNILDI_07000 0.53 -1.1 1,401,157 + nsrR BNILDI_07000 0.53 -0.7 1,401,158 - nsrR BNILDI_07000 0.53 -2.0 1,401,226 + nsrR BNILDI_07000 0.69 -0.4 1,401,296 + nsrR BNILDI_07000 0.85 +0.3 1,401,335 + -0.4 1,401,337 + -0.3 1,401,337 + +0.3 1,401,342 + +1.5 1,401,453 + -0.4 1,401,708 + rnr BNILDI_07005 0.13 -0.9 1,401,816 + rnr BNILDI_07005 0.17 +0.3 1,401,862 + rnr BNILDI_07005 0.19 +0.7 1,401,922 - rnr BNILDI_07005 0.21 -0.4 1,401,927 - rnr BNILDI_07005 0.22 +0.1 1,402,100 - rnr BNILDI_07005 0.29 -0.7 1,402,307 - rnr BNILDI_07005 0.37 -0.8 1,402,309 + rnr BNILDI_07005 0.37 +2.9 1,402,309 + rnr BNILDI_07005 0.37 -1.4 1,402,309 + rnr BNILDI_07005 0.37 +0.3 1,402,309 + rnr BNILDI_07005 0.37 +0.2 1,402,310 - rnr BNILDI_07005 0.37 +0.3 1,402,310 - rnr BNILDI_07005 0.37 +0.5 1,402,310 - rnr BNILDI_07005 0.37 -0.8 1,402,310 - rnr BNILDI_07005 0.37 -0.6 1,402,310 - rnr BNILDI_07005 0.37 -1.4
Or see this region's nucleotide sequence