Experiment: Bas61
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt asd and rsgA are separated by 96 nucleotides rsgA and orn are separated by 94 nucleotides orn and Gly_trna are separated by 210 nucleotides Gly_trna and Gly_trna are separated by 36 nucleotides
BNILDI_06905: asd - archaetidylserine decarboxylase, at 1,384,134 to 1,385,102
asd
BNILDI_06910: rsgA - small ribosomal subunit biogenesis GTPase RsgA, at 1,385,199 to 1,386,251
rsgA
BNILDI_06915: orn - oligoribonuclease, at 1,386,346 to 1,386,891
orn
BNILDI_06920: Gly_trna - tRNA-Gly(gcc), at 1,387,102 to 1,387,177
_trna
BNILDI_06925: Gly_trna - tRNA-Gly(gcc), at 1,387,214 to 1,387,289
_trna
Position (kb)
1385
1386
1387 Strain fitness (log2 ratio)
-1
0
1
2 at 1384.249 kb on - strand, within asd at 1385.145 kb on - strand at 1385.145 kb on - strand at 1385.189 kb on - strand at 1385.194 kb on - strand at 1385.194 kb on - strand at 1386.292 kb on - strand at 1386.297 kb on - strand at 1386.318 kb on + strand at 1386.318 kb on + strand at 1386.909 kb on + strand at 1386.909 kb on + strand at 1386.910 kb on - strand at 1387.078 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas61 remove 1,384,249 - asd BNILDI_06905 0.12 +0.1 1,385,145 - +1.9 1,385,145 - +0.2 1,385,189 - +0.3 1,385,194 - -0.1 1,385,194 - +0.2 1,386,292 - -0.2 1,386,297 - +0.3 1,386,318 + -0.3 1,386,318 + +0.7 1,386,909 + -1.3 1,386,909 + +0.2 1,386,910 - -0.1 1,387,078 + -1.1
Or see this region's nucleotide sequence