Experiment: Bas61
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ligB and gmk are separated by 257 nucleotides gmk and rpoZ are separated by 54 nucleotides rpoZ and spoT are separated by 18 nucleotides
BNILDI_04065: ligB - NAD-dependent DNA ligase LigB, at 801,143 to 802,825
ligB
BNILDI_04070: gmk - guanylate kinase, at 803,083 to 803,706
gmk
BNILDI_04075: rpoZ - DNA-directed RNA polymerase subunit omega, at 803,761 to 804,036
rpoZ
BNILDI_04080: spoT - bifunctional GTP diphosphokinase/guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase, at 804,055 to 806,163
spoT
Position (kb)
803
804
805 Strain fitness (log2 ratio)
-2
-1
0
1 at 802.795 kb on + strand at 802.795 kb on + strand at 802.799 kb on + strand at 802.800 kb on - strand at 802.800 kb on - strand at 802.800 kb on - strand at 802.800 kb on - strand at 802.800 kb on - strand at 802.800 kb on - strand at 802.800 kb on - strand at 802.802 kb on + strand at 802.802 kb on + strand at 802.817 kb on + strand at 802.840 kb on + strand at 802.857 kb on - strand at 802.858 kb on + strand at 802.858 kb on + strand at 802.858 kb on + strand at 802.859 kb on - strand at 802.859 kb on - strand at 802.859 kb on - strand at 802.859 kb on - strand at 802.859 kb on - strand at 802.859 kb on - strand at 802.859 kb on - strand at 802.859 kb on - strand at 802.859 kb on - strand at 802.936 kb on + strand at 803.007 kb on + strand at 803.008 kb on - strand at 803.042 kb on + strand at 803.053 kb on + strand at 803.720 kb on - strand at 803.756 kb on + strand at 803.760 kb on + strand at 803.760 kb on + strand at 803.903 kb on + strand, within rpoZ
Per-strain Table
Position Strand Gene LocusTag Fraction Bas61 remove 802,795 + -1.6 802,795 + -0.1 802,799 + -0.9 802,800 - +0.4 802,800 - -0.5 802,800 - -1.5 802,800 - -0.8 802,800 - -0.1 802,800 - +0.9 802,800 - +0.0 802,802 + -0.7 802,802 + -1.2 802,817 + -0.2 802,840 + -2.2 802,857 - +0.0 802,858 + +1.0 802,858 + +0.7 802,858 + -1.9 802,859 - -0.3 802,859 - -0.1 802,859 - +0.4 802,859 - -1.0 802,859 - -0.8 802,859 - +0.1 802,859 - -0.0 802,859 - -0.4 802,859 - +0.6 802,936 + +1.2 803,007 + -0.1 803,008 - +0.6 803,042 + +0.0 803,053 + +0.2 803,720 - -1.8 803,756 + -0.0 803,760 + +1.0 803,760 + -0.4 803,903 + rpoZ BNILDI_04075 0.51 +0.2
Or see this region's nucleotide sequence