Experiment: Bas61
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt kbaZ and agaV are separated by 22 nucleotides agaV and agaW are separated by 10 nucleotides agaW and agaE overlap by 11 nucleotides agaE and agaF are separated by 17 nucleotides
BNILDI_01415: kbaZ - tagatose-bisphosphate aldolase subunit KbaZ, at 279,934 to 281,214
kbaZ
BNILDI_01420: agaV - PTS N-acetylgalactosamine transporter subunit IIB, at 281,237 to 281,710
agaV
BNILDI_01425: agaW - PTS N-acetylgalactosamine transporter subunit IIC, at 281,721 to 282,500
agaW
BNILDI_01430: agaE - PTS N-acetylgalactosamine transporter subunit IID, at 282,490 to 283,368
agaE
BNILDI_01435: agaF - PTS galactosamine/N-acetylgalactosamine transporter subunit IIA, at 283,386 to 283,820
agaF
Position (kb) 281
282
283 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 280.730 kb on - strand, within kbaZ at 280.977 kb on + strand, within kbaZ at 280.977 kb on + strand, within kbaZ at 280.978 kb on - strand, within kbaZ at 280.978 kb on - strand, within kbaZ at 280.978 kb on - strand, within kbaZ at 280.978 kb on - strand, within kbaZ at 280.981 kb on + strand, within kbaZ at 281.166 kb on + strand at 281.167 kb on - strand at 281.167 kb on - strand at 281.189 kb on - strand at 281.213 kb on - strand at 281.222 kb on - strand at 281.236 kb on + strand at 281.245 kb on + strand at 281.246 kb on - strand at 281.246 kb on - strand at 281.554 kb on + strand, within agaV at 281.627 kb on - strand, within agaV at 281.804 kb on + strand, within agaW at 281.845 kb on + strand, within agaW at 281.846 kb on - strand, within agaW at 281.954 kb on + strand, within agaW at 281.978 kb on + strand, within agaW at 282.007 kb on + strand, within agaW at 282.050 kb on + strand, within agaW at 282.141 kb on + strand, within agaW at 282.142 kb on - strand, within agaW at 282.142 kb on - strand, within agaW at 282.172 kb on - strand, within agaW at 282.195 kb on - strand, within agaW at 282.198 kb on + strand, within agaW at 282.198 kb on + strand, within agaW at 282.199 kb on - strand, within agaW at 282.333 kb on + strand, within agaW at 282.333 kb on + strand, within agaW at 282.334 kb on - strand, within agaW at 282.334 kb on - strand, within agaW at 282.440 kb on + strand at 282.440 kb on + strand at 282.498 kb on + strand at 282.498 kb on + strand at 282.499 kb on - strand at 282.568 kb on + strand at 282.641 kb on + strand, within agaE at 282.641 kb on + strand, within agaE at 282.641 kb on + strand, within agaE at 282.654 kb on + strand, within agaE at 282.654 kb on + strand, within agaE at 282.658 kb on + strand, within agaE at 282.658 kb on + strand, within agaE at 282.658 kb on + strand, within agaE at 282.658 kb on + strand, within agaE at 282.667 kb on - strand, within agaE at 282.688 kb on + strand, within agaE at 282.850 kb on - strand, within agaE at 282.970 kb on - strand, within agaE at 282.990 kb on + strand, within agaE at 282.991 kb on - strand, within agaE at 282.991 kb on - strand, within agaE at 283.011 kb on - strand, within agaE at 283.042 kb on + strand, within agaE at 283.156 kb on + strand, within agaE at 283.156 kb on + strand, within agaE at 283.156 kb on + strand, within agaE at 283.156 kb on + strand, within agaE at 283.157 kb on - strand, within agaE at 283.157 kb on - strand, within agaE at 283.207 kb on - strand, within agaE at 283.207 kb on - strand, within agaE at 283.211 kb on + strand, within agaE at 283.212 kb on - strand, within agaE at 283.224 kb on + strand, within agaE at 283.224 kb on + strand, within agaE at 283.384 kb on - strand at 283.394 kb on + strand at 283.395 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas61 remove 280,730 - kbaZ BNILDI_01415 0.62 -0.5 280,977 + kbaZ BNILDI_01415 0.81 +0.1 280,977 + kbaZ BNILDI_01415 0.81 +1.3 280,978 - kbaZ BNILDI_01415 0.81 +0.9 280,978 - kbaZ BNILDI_01415 0.81 -0.9 280,978 - kbaZ BNILDI_01415 0.81 -0.1 280,978 - kbaZ BNILDI_01415 0.81 -0.2 280,981 + kbaZ BNILDI_01415 0.82 -0.1 281,166 + +0.1 281,167 - -0.7 281,167 - +0.6 281,189 - +0.1 281,213 - -1.7 281,222 - +0.6 281,236 + -0.9 281,245 + -0.1 281,246 - -0.2 281,246 - +1.4 281,554 + agaV BNILDI_01420 0.67 -0.3 281,627 - agaV BNILDI_01420 0.82 +0.6 281,804 + agaW BNILDI_01425 0.11 +1.2 281,845 + agaW BNILDI_01425 0.16 +1.2 281,846 - agaW BNILDI_01425 0.16 +1.9 281,954 + agaW BNILDI_01425 0.30 -1.4 281,978 + agaW BNILDI_01425 0.33 +0.1 282,007 + agaW BNILDI_01425 0.37 +0.9 282,050 + agaW BNILDI_01425 0.42 +0.1 282,141 + agaW BNILDI_01425 0.54 +2.9 282,142 - agaW BNILDI_01425 0.54 -0.1 282,142 - agaW BNILDI_01425 0.54 -0.1 282,172 - agaW BNILDI_01425 0.58 -0.5 282,195 - agaW BNILDI_01425 0.61 -0.1 282,198 + agaW BNILDI_01425 0.61 -0.4 282,198 + agaW BNILDI_01425 0.61 -0.1 282,199 - agaW BNILDI_01425 0.61 -0.2 282,333 + agaW BNILDI_01425 0.78 +0.1 282,333 + agaW BNILDI_01425 0.78 -0.4 282,334 - agaW BNILDI_01425 0.79 -0.2 282,334 - agaW BNILDI_01425 0.79 -0.1 282,440 + +0.0 282,440 + -0.4 282,498 + -0.4 282,498 + -0.2 282,499 - +0.8 282,568 + +0.8 282,641 + agaE BNILDI_01430 0.17 +0.3 282,641 + agaE BNILDI_01430 0.17 +0.5 282,641 + agaE BNILDI_01430 0.17 +0.6 282,654 + agaE BNILDI_01430 0.19 +1.4 282,654 + agaE BNILDI_01430 0.19 -1.2 282,658 + agaE BNILDI_01430 0.19 +0.3 282,658 + agaE BNILDI_01430 0.19 +0.2 282,658 + agaE BNILDI_01430 0.19 -0.8 282,658 + agaE BNILDI_01430 0.19 -0.3 282,667 - agaE BNILDI_01430 0.20 +0.6 282,688 + agaE BNILDI_01430 0.23 +0.3 282,850 - agaE BNILDI_01430 0.41 +0.1 282,970 - agaE BNILDI_01430 0.55 -0.1 282,990 + agaE BNILDI_01430 0.57 +0.7 282,991 - agaE BNILDI_01430 0.57 +1.0 282,991 - agaE BNILDI_01430 0.57 +0.8 283,011 - agaE BNILDI_01430 0.59 -1.0 283,042 + agaE BNILDI_01430 0.63 +0.1 283,156 + agaE BNILDI_01430 0.76 +1.1 283,156 + agaE BNILDI_01430 0.76 +0.1 283,156 + agaE BNILDI_01430 0.76 +0.4 283,156 + agaE BNILDI_01430 0.76 -0.4 283,157 - agaE BNILDI_01430 0.76 -0.0 283,157 - agaE BNILDI_01430 0.76 -0.3 283,207 - agaE BNILDI_01430 0.82 -0.9 283,207 - agaE BNILDI_01430 0.82 -2.6 283,211 + agaE BNILDI_01430 0.82 +0.2 283,212 - agaE BNILDI_01430 0.82 -0.3 283,224 + agaE BNILDI_01430 0.84 -0.6 283,224 + agaE BNILDI_01430 0.84 +0.8 283,384 - +0.1 283,394 + -0.2 283,395 - -0.5
Or see this region's nucleotide sequence